630nm和514.5nm两种波长激光束对人体胃、肺癌细胞株的HpD光敏化光剂量的研究

本工作使用630nm和514.5nm两种波长激光束对不同分化程度的人胃、肺癌细胞株进行HpD光敏化光剂量的比较研究。实验数据显示,514.5nm波长光对体外培养单层癌细胞的光敏杀伤力是630nm光的2—3倍。分析结果还表明,分化程度越低的癌细胞受光敏化的效率越高

从预防事故和消除隐患的角度改进实验室安全工作

从预防实验室事故和消除安全隐患的角度,分析归纳出安全意识淡薄、安全知识缺乏、安全条例执行不到位、工作人员心理疲劳等导致实验室事故和安全隐患的深层次原因,以及这些原因之间的相互影响,并从安全教育教学、人事考核、实验室团队建设、信息化建设等方面对实验室安全工作提出相应的改进对策与建议

Studied the difference of genetic feature between tissue cultured and cultivated paddy rice of Jia He Zao Zan via microsatellite(SSR) markers

株高是水稻重要的农艺形状之一,植株过高将导致倒伏和减产,目前,很多新的技术究被用来鉴定,图位克隆与水稻株高相关的基因及机理的研究,本实验选择优质早籼稻佳禾早占种植苗和经过组培获得的矮化突变水稻为材料,为研究比较它们间的遗传物质上差异,根据康耐尔大学的资料设计了311对SSR引物对佳禾早占种植材料和组培材料进行分析,对两种材料进行PCR多态性扩增,结果发现两者间存在多态性的引物有88对,多态性比例达到30.3%。在矮杆材料中不但验证了已报道的11个与调控株高性状基因相连锁的标记连锁群,同时在第3号染色体和第9号染色体上还获得了两个以前基本未有报道的标记集中分布区域。结果说明,该培养基培育出的佳禾早占水稻后代所表现出的矮杆性状与亲本在遗传物质上确有明显差别。该结果有助于挖掘和定位新的矮杆基因,并有利于今后在水稻育种中进行水稻株高性状的控制,同时也为开展矮化性状机理的研究提供有利的实验材料。Plant height is one of the important agronomic traits of rice.Overhigh plant easily led to lodge and reduce output.Recently,great advances have been made in the identification,mapping,cloning and action mechanism of plant height genes in rice.This present paper selected the tissue cultured and cultivated paddy rice of Jia He Zao Zan(J.H.Z.Z) as experimental object,which were significant different on the height,the tissue cultured plant was only less than half height of the cultivated plant.In order to compare their differences of genetic and determine whether there are relationship between the dwarf gene and the dwarf character of tissue cultured plant,Polymorphism analysis was conducted between them by 311 pairs of rice SSR primers,designed by Cornell University,the result indicated that 88 pairs of primers had polymorphism between two kinds of materials,the ration in total primers was about 30.3%,the experimental result not only verified 11 multiple groups which regulate the height gene,but also obtained two group regions which control the dwarf character on chromosome 3 and chromosome 9,which have not been reported previously,these results will help to explore and determine the situation of new dwarf gene,and will be of benefit to act genetic manipulation of plant height of in rice breeding.These results also provided the evidence for studying the mechanism of dwarf.国家863项目(2002AA211091),(20001AA11091);; 厦门大学细胞生物学和肿瘤细胞工程教育部重点实验室开放基金资助(NO:2005108)资

Linux平台下基于Intel千兆网卡的零拷贝技术的研究与实现

千兆网络时代的到来,使得在通用操作系统下远达不到线速捕获和发送数据包。本文实现了一种在用户空间直接控制网卡收发数据包的零拷贝平台,旁路了内核,减少了数据包在内核和用户空间传递过程中的拷贝,缓存,以及系统调用等的开销,使得采用轮询机制基本上达到了千兆网卡的线速。中山市科技项目(2006A157);; 厦门大学院士基金(0630-E23011);; 厦门大学新世纪优秀人才基金(0000-X07116

Study on Rhizodegradation of Soluble Protein by Sesuvium portulacastrum Linn.

采用水培法研究了海马齿(SESuVIuM POrTulACASTruM lInn.)与其根际微生物对可溶性蛋白质的联合降解作用.分别设置抑菌和不抑菌的植物组、细菌组和对照组并连续添加蛋白质,测定了降解过程中蛋白质的浓度、细菌密度和亮氨酸氨基肽酶活性的变化.结果显示,在不抑菌实验中,植物组的蛋白质浓度下降最快.36H后,植物组、细菌组和对照组的降解率分别是97%、41%和26%.蛋白质的降解过程伴随着细菌数量的增加和亮氨酸氨基肽酶活性的升高,植物组的亮氨酸氨基肽酶活性始终高于细菌组.24H后,植物组的亮氨酸氨基肽酶活性达到最高值0.75μMOl/(l·H),此后酶活性下降至0.22μMOl/(l·H).细菌组的亮氨酸氨基肽酶活性最高值出现在60H,达到0.43μMOl/(l·H),至结束时降低为0.说明海马齿与根际细菌联合作用下,胞外酶活性升高,蛋白质降解效率最快.在抑菌实验中,抗生素有效抑制了细菌的生长,3个处理组的蛋白质变化趋势基本一致,实验过程中检测不到亮氨酸氨基肽酶,说明单纯的海马齿根系不分泌该胞外酶.研究表明,在蛋白质的根际降解过程中,根际细菌是亮氨酸氨基肽酶的分泌者和蛋白质降解的主要执行者,但根际细菌对蛋白质的高效降解必须以海马齿根系为依托.本研究结果为更好地利用海马齿生态浮床修复海水环境中的有机氮提供了理论依据.Phytoremediation of soluble protein by marine halophyte Sesuvium portulacastrum which has been supposed to use in ecofloating bed systems was studied through a hydroponic culture approach.The plant group,the bacteria group and the control group were treated with and without antibiotic addition.The results showed that,without antibiotic addition,each time after bovine serum albumin(BSA)was added,the BSA concentration of the plant group dropped fastest.After 36hincubation,the degradation rate of the plant group,the bacteria group and the control group was 97%,41%and 26%respectively,i.e.the degradation rate of the plant group was significantly higher than the bacteria group.Protein degradation associated with the increasing of both leucine amino peptidase activity(LAPA)and bacterial density,and LAPA of the plant group was always higher than that of the bacteria group.Soon after the experiment started,LAPA of the plant group increased rapidly and reached its maximum value of 0.75μmol/(L·h)at 24h.Then LAPA started to decline to 0.22μmol/(L·h)till the experiment ended.The maximum LAPA value of the bacteria group was 0.43μmol/(L·h)at 60h,and till the experiment ended,the LAPA was 0μmol/(L·h).With antibiotic addition,the bacterial density of both plant group and bacteria group showed downtrend,and it showed that the antibiotic inhibited the growth of bacteria effectively.The degradation rates of 3groups were almost the same,and LAPA was not detected in the procession.It was suggested that in the procession of rhizodegradation of BSA by S.portulacastrum,the extracellular enzyme was produced by rhizosphere bacteria,and the rhizoshpere microbe was the main performer of BSA degradation.Besides,the high degradation rates of rhizosphere bacteria relied on the root of S.portulacastrum.The results provide meaningful insights into the interrelationship between microbes and plant as well as the practical significance of soluble protein rhizodegradation in seawater environments by eco-floating bed system.国家海洋公益性行业科研专项(201205009); 深圳市科技研发资金(JCYJ20120830153030587

厦门会展业竞争力分析

会展业是近年来迅速发展的第三产业，从原始社会的物物交换开始萌芽，随着科学技术和现代信息技术的进步和推广，逐渐发展成为现代会展；不仅能带来实际的经济效益，还能推动有关产业的发展进步，使会展业逐渐受到了更广泛的关注。会展业的发展能为厦门经济注入新的活力，也带来可观的经济效益。本文概括厦门会展业四十年的发展进程，探讨厦门会展与厦门经济发展的关系，并用波特钻石模型对厦门会展业的竞争力进行分析，从厦门会展业的现状，提出适合厦门提高会展竞争力的建议，以增强厦门会展的吸引力并促进厦门经济更好发展。</jats:p

基于Pro/E和ANSYS的摆线齿轮泵模态分析

运用三维CAD软件Pro/E建立了某型摆线齿轮泵三维几何模型,然后用Hypermesh对其进行网格划分,简化并导入ANSYS软件中,建立了有限元模型。利用ANSYS软件对摆线齿轮泵进行了模态分析,获得摆线齿轮泵的振型、固有频率和振型参与系数,确保在设计摆线齿轮泵时能够避免模型发生共振。分析结果表明摆线齿轮泵的结构设计合理,而且模态分析的结果可以为新产品的开发和结构优化设计提供重要的参考依据

Novel Electrochemical Method for Synthesis of Nanostructured Materials of Noble Metals Based on Poly(N-Vinylpyrrolidone) (PVP)

以不同聚合度的聚乙烯吡咯烷酮(PVP)作为金纳米团簇的稳定剂和形状控制剂,应用电化学还原方法制备尺寸可控的金纳米晶体.借助PVP聚合物的动态伸缩和卷曲特性将电化学还原得到的金纳米粒子前驱体组装成线状和环状的纳米粒子聚集体,再由不稳定前驱体粒子的定向聚集制备厚度为几十纳米的金纳米棱柱.并用分步电化学还原法合成核壳结构的金银纳米复合粒子.本文为制备不同形状和结构的贵金属纳米结构材料提供了一种可行的电化学合成新方法.Gold nanocrystals with controllable size were synthesized through a simple electroreduction method using poly(N-vinylpyrrolidone)(PVP) of different polymerization degree as both stabilizer and shape-controller for gold nanoclusters.Individual gold nanoparticles spontaneously assembled into the wire-and ring-shaped nanoparticle aggregates depending on the PVP's dynamic stretching and curling,and precursor nanoparticles grew into flat,single-crystalline nanoprisms with thickness of dozens of nanometers based on the oriented aggregation between particles.Besides,core-shell Au/Ag nanoparticles were conveniently synthesized through two-step electroreduction of noble metal ions in the presence of PVPK30.The electrochemical synthetic method based on PVP provides a new path for preparation of noble metal nanostructured materials with different shape and structure.作者联系地址：山东大学化学与化工学院,山东师范大学理化分析测试中心,哈尔滨工业大学(威海)海洋学院,山东大学化学与化工学院,山东大学化学与化工学院,山东大学化学与化工学院 山东济南250100,山东济南250014,山东威海264209,山东济南250100,山东济南250100,山东济南250100Author's Address: 1,ZHANG Xiao-kai~2,HUANG Ming-hu~3,HUANG Shao-xin~1,FENG Xing-li~1,MA Hou-yi~*11.School of Chemistry and Chemical Engineering,Shandong University,Jinan 250100,Shandong,China,2.Analysis and Testing Center,Shandong Normal University,Jinan 250014,Shandong,China,3.School of the Ocean,Harbin Institute of Technology at Weihai,Weihai 264209, Shandong,Chin