Cloning, expression and proteolytic activity analysis of apoptosis-related abCaspase gene in Haliotis diversicolor

鲍的味道鲜美、营养丰富，具有很高的经济价值，是我国重要的海水养殖产品之一。近些年来规模化养殖带来的鲍流行性疾病时有发生，但迄今没有可行的免疫预防措施，因此深入研究鲍的免疫机制是鲍养殖业健康发展的迫切需求。本实验室在前期研究中，利用抑制性差减杂交技术(SSH)从杂色鲍血淋巴细胞中筛选发现了一个与细胞凋亡调控蛋白酶相关的基因片段，与哺乳动物的caspase基因家族具有同源性。已知哺乳动物caspase是细胞凋亡与炎症反应过程中的重要功能蛋白酶，参与清除异物包括病原微生物等的免疫反应。为深入研究caspase在鲍抗细菌免疫中的作用，本论文较系统地研究了该基因的完整基因序列、基因结构组成、组织器官特异...Abalone is one of the most economically important species in aquaculture. However, increasing prevalence of bacterial diseases has become an important impairment for the sustainable development of cultured abalone stocks. Thus, investigation into the abalone immune system is becoming very important as only little is known about the cellular immune response of marine gastropods. In our previous stu...学位：理学博士院系专业：海洋与环境学院环境科学与工程系_环境科学学号：2122005140319

Development of the viviparous hypocotyl of mangrove and its adaptation to inter-tidal habitats:A review

作者简介: 闫中正( 1978～) , 男, 河南南阳人, 硕士生, 主要从事红树植物生理生态学研究。E-mail : nytv@ sohu. com 通讯作者Author for correspondence. E-mail: wenqing2001@ hotmail. com[中文文摘]热带亚热带海岸潮间带的高盐、淹水、土壤缺氧和潮水冲击等不良环境因子导致了红树植物在形态、生理和生态方面的特异性 ,尤其是其独特的胎生现象。从红树植物胎生繁殖体在母体的发育过程和繁殖体落地后对潮间带生境的适应两个角度探讨了红树植物胎生现象的适应意义。红树植物的胎生现象是对潮间带盐胁迫、淹水、土壤缺氧和潮水冲击等综合生境的适应 ,而不是单独其中一种胁迫因子的适应。红树植物胎生繁殖体在母体的发育过程中通过能量积累、渗透调节、形态完善等过程为掉落以后适应潮间生境做准备 ,掉落的胎生繁殖体依靠从母体获得的能量和营养克服潮间带的盐胁迫、淹水、土壤缺氧和潮水冲击等多重胁迫。红树植物胎生繁殖体落地后的能量收支平衡是决定繁殖体定居成功与否的关键 ,而胎生现象的意义正是通过胎生繁殖体在母体发育过程中积累足够的能量和营养来适应潮间带生境。红树植物胎生繁殖体落地后苗木在潮间带滩涂的生长发育过程中存在一个能量“阈限”,能阈的高低决定于潮间带生境的恶劣程度 ,而突破能阈能力决定于胎生繁殖体所携带的能量与营养的数量。这为红树林湿地恢复提供了理论基础 ,但还需要更多的证据来支持该理论.[英文文摘]Mangroves are a diverse group of predominantly tropical trees and shrubs growing in the marine intertidal zone where conditions are usually harsh , restrictive and dynamic. The peculiarities of the mangrove intertidal habitat causes the peculiarity of its structure, physiology and ecology . The vivipary of mangroves is one particular feature which is the result of long-term adaptation to the inter-tidal environment. The adaptive significa nce of vivipary to mang rove plants was reviewed from two aspects: propagule development while still attached on the mat ernal plants and adaptation to inter-tidal environments of propagules after release . Vivipary in mangrove plants is an adaptation to the integ rat ed stresses of salt, water logging and tide,but not any single stress. During t he development of vivipar ous propag ules while still attached to maternal plants, they accumulate energy , nutrients and osmotica and perfect their structure, adaptating them to t he intertidal environment after release. The viviparous propagules depend on the energy and nutrients accumulated from maternal plants to cope with salt and waterlogging stresses. The success or not of the ecesis of vivipar ous propagules depends on the balance of energy income and expenditure . The significance of vivipary in mang rove plants is to accumulate enough energy and nutrients to cope with the intertidal environment. A “t hr eshold”hypothesis is put forw ard in this paper . According to this hypothesis, there is a threshold of energy during growth and development of the viviparous propagules in the intertidal environment . The height of the threshold depends on t he degree of environmental stress, such as water salinity, w aterlogging duration and photo synthetic illuminat ion. The ability to climb above the threshold depends on storing sufficient quantity of energy and nutrients in viviparous propagules. This threshold hypothesis calls for more direct evidence .国家自然科学基金资助项目 (3 0 2 0 0 0 3 1

Effects of salinity on the growth and proline,soluble sugar and protein contents of Spartina alterniflora

研究了互花米草在不同培养盐度下,株高、茎粗、叶长、叶宽、叶面积、鲜重等生长指标以及脯氨酸、可溶性糖和蛋白质含量的变化情况。结果表明,随着盐度增加,米草株高呈下降趋势,在高盐度(5 0‰)下,米草叶面积、叶长等指标与对照组相比明显下降,鲜重与低盐度组比较显著下降。米草叶片中可溶性糖、脯氨酸含量随盐度增加总体上呈上升趋势;可溶性蛋白质含量随盐度增加亦呈上升趋势,表明蛋白质合成增强。在盐胁迫下,渗透调节物质的积累作用是互花米草对盐胁迫的主要响应过程,可以作为其抗盐性的生理参数。 【英文摘要】 Under different salinity conditions,variations of height,stem diameter,leaf length and width,leaf area,fresh weight,proline,soluble sugar and protein contents of Spartina alterniflora seedlings were investigated.The results showed that the height tended to decrease with the increaseing salinity in the medium.In high salinity (50‰) treatment,the leaf area and length decreased significantly compared with control,and single plant fresh weight decreased more than those of low salinity treatments.The sugar ...国家自然科学基金项目 (3 0 2 710 65 ,3 9970 43 8,3 9870 63 0 );; 福建省自然科学基金资助项目 (D0 2 10 0 0 1

α-羟丁酸脱氢酶与乳酸脱氢酶比值的临床意义

目的探讨α-羟丁酸脱氢酶与乳酸脱氢酶比值对心肌梗死的诊断价值。方法采用470例血清HBDH升高患者血清,分为心肌梗死组、脑部疾病组、肺部疾病组、肝脏疾病组、肾脏疾病组,测定血清中α-羟丁酸脱氢酶与乳酸脱氢酶比值。同时,设正常对照组。结果心肌梗塞组及脑部疾病组HBDH/LDH比值明显升高,与正常对照组有着显著性差异(P0.05)。结论以HBDH/LDH的比值诊断心肌梗塞较之单纯用HBDH或LDH诊断特异性高

Construction of SSH library from haemocyte of variously colored abalone challenged with bacteria and differential expression analysis of macrophage expressed protein

以雌性杂色鲍为对象,以大肠杆菌、副溶血弧菌、溶壁微球菌、表皮葡萄球菌、金黄色葡萄球菌的混悬液做为攻毒菌,利用抑制性差减杂交(SSH)技术构建细菌攻毒的杂色鲍血淋巴细胞SSHcDNA文库。随机挑取生长菌落110个克隆子,进行菌液PCR鉴定,计算文库重组率为98.18%,文库容量为1.37×106pfu。将重组子测序,经BLAST一致性搜索比对分析,有一重组片段含有穿孔素(Perforin)保守结构域,为巨噬细胞表达蛋白(MEP)类穿孔素部分cDNA序列,片段大小为1551bp,连续编码517个氨基酸残基,申请GenBank登录号为EU272049。经半定量PCR和荧光定量PCR差异显示分析,发现在细菌感染状态下MEP基因在血淋巴细胞中存在明显的上调表达现象。Abalones are considered to be the most precious delicacy from the sea, and become very important commercial seafood in aquaculture worldwide. Variously colored abalone (Haliotis diversicolor Reeve, 1846) has been widely cultured on the southeast coast for more than twenty years. However, abalone culture frequently suffers from bacterial infection and mass mortality of reared abalones causes serious economic losses. Unfortunately, knowledge of the defense mechanism in this animal is still lacking. In this study, using suppression subtractive hybridization (SSH) technology, a forward SSH li-brary was constructed from haemocytes of H. diversicolor, with the content of 1.37×106 pfu and the recombinant rate of98.18%. After the recombinant plasmids were sequenced, partial cDNA of macrophage expressed protein (MEP) was recognized based on BLAST searches in NCBI, with the size of 1 551 bp, and continuously encoding 517 amino acids. Semi-quantitative PCR and quantitative real-time PCR results showed that MEP cDNA was distinctly up-regulated in haemocytes of the bacterial-challenged group compared to the unchallenged group. The gene information obtained from this library will provide new insights into the immune mechanism of H. diversicolor and facilitate future study of target genes involved in the response to invading microorganisms.国家高技术研究发展计划项目(863计划)(编号:2007AA091406)资助~

Effects of Salinity on Oxidative and Antioxidative System of Spartina alterniflora

研究了互花米草幼苗在不同盐度培养下,其叶片超氧阴离子(O2-)、过氧化氢(H2O2)、丙二醛(MDA)、谷胱苷肽(GSH)、抗坏血酸(AsA)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(AsA-POD)、过氧化氢酶(CAT)等氧化和抗氧化指标的变化.结果表明,各指标在不同盐度间都有显著差异(p<0.05).经研究发现,随着盐度的升高,O2-含量有所下降,H2O2含量稍有上升,膜脂过氧化产物MDA含量虽有上升,但幅度很小且保持在一个较低的水平,而各抗氧化保护酶的活性和小分子抗氧化剂GSH和AsA的含量都升高了,且都保持在较高的水平,说明互花米草是一种耐盐性较高的滨海盐沼植物,在盐胁迫下,其体内的抗氧化保护酶和抗氧化剂在清除O2-的过程中发挥了重要的作用. 【英文摘要】 In this paper,the changes of H_2O_2,O~-_2,MDA,GSH,AsA contents and SOD,POD,AsA-POD,CAT activities for Spartina alterniflora seedlings under different salinity treatments were studied.The results showed that all indices changed significantly among various salinities (p<0.05).It was also found that the content of O~-_2 declined with the increase of salinity,while that of H_2O_2 increased slightly.The content of MDA,a product of lipid peroxidation,also went up slightly and remained a relatively-low level,while...福建省自然科学基金(D0210001);; 国家自然科学基金(30271065,39970438,39870630)资

Effects of salinity on the growth and proline,soluble sugar and protein contents of Spartina alterniflora

研究了互花米草在不同培养盐度下,株高、茎粗、叶长、叶宽、叶面积、鲜重等生长指标以及脯氨酸、可溶性糖和蛋白质含量的变化情况。结果表明,随着盐度增加,米草株高呈下降趋势,在高盐度(5 0‰)下,米草叶面积、叶长等指标与对照组相比明显下降,鲜重与低盐度组比较显著下降。米草叶片中可溶性糖、脯氨酸含量随盐度增加总体上呈上升趋势;可溶性蛋白质含量随盐度增加亦呈上升趋势,表明蛋白质合成增强。在盐胁迫下,渗透调节物质的积累作用是互花米草对盐胁迫的主要响应过程,可以作为其抗盐性的生理参数。Under different salinity conditions,variations of height,stem diameter,leaf length and width,leaf area,fresh weight,proline,soluble sugar and protein contents of Spartina alterniflora seedlings were investigated.The results showed that the height tended to decrease with the increaseing salinity in the medium.In high salinity (50‰) treatment,the leaf area and length decreased significantly compared with control,and single plant fresh weight decreased more than those of low salinity treatments.The sugar and proline contents in leaves generally increased with salinity.The content of protein also increased with salinity,which implied the increase in protein synthesis.Accumulation of osmoprotectants was the key response of Spartina alterniflora under salinity stress,and it could be characterized as an indicator of salt tolerance.国家自然科学基金项目 (3 0 2 710 65 ,3 9970 43 8,3 9870 63 0 );; 福建省自然科学基金资助项目 (D0 2 10 0 0 1