The Construction of a Vector for Endosperm-specific Expression of IPT Gene and It's Transformation in Rice

为研究细胞分裂素在水稻籽粒发育中的作用,构建了P1300-P Pg-5α-IPT-nOS植物表达载体,并对水稻进行遗传转化。以水稻品种9311为材料,采用PCr法获得种子中特异表达的Pg-5α基因启动子,并将此启动子与P1300相连接,构建P1300-P Pg-5α载体,用nCOⅠ和SPEⅠ双酶切P Sg516,获得IPT-nOS核酸片段,然后将此核酸片段插入到P1300-P Pg-5α中,构建P1300-P Pg-5α-IPT-nOS植物表达载体。以水稻品种日本晴愈伤组织为材料,采用农杆菌介导的方法进行遗传转化。成功构建了植物表达载体P1300-P Pg-5α-IPT-nOS,获得了阳性率为81.3%转IPT基因群体。获得转基因株系后,将为进一步筛选高效表达株系及观察其籽粒生长发育过程提供基础。In order to specifically express IPT gene in the endosperm of developing rice seed,the binary vector p1300-p PG-5α-IPT-Nos was constructed,firstly,the promoter of PG-5α was obtained by PCR amplification from rice genomic DNA( variety: 9311) and p1300-p PG-5α was constructed by inserting p PG-5α into p1300,then the IPT-Nos fragment was obtained by cutting p SG516 with Nco Ⅰ and Spe Ⅰ.After IPT-Nos inserted into p1300-p PG-5α,p1300-p PG-5α-IPT-Nos was constructed.p PG-5α-IPT-Nos was transformated into Oryza sativa( subsp.japonicacv.Nipponbare) cells by agrobacterium mediated method and regenerative seedlings were obtained.The results of PCR showed that about 81.3% seedlings were positive.唐山师范学院博士基金项目(09A01); 农业部“948”项目(2011-G1(3)-07); 河北省科技计划项目(13396401D

Self-assembly, thermal stability and photoluminescence of two mixed-ligand silver(I) networks via 2D -> 2D and 2D -> 3D parallel interpenetration of (4,4) nets

通讯作者地址: Huang, RB (通讯作者), Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 2. Xiamen Univ, State key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] mixed-ligand silver(I) coordination polymers (CPs) [Ag-2(dmapym)(2)(suc)center dot H2O](n) (1) and [Ag-2(dmapym)(2)(bdc)center dot 2H(2)O](n) (2), (dmapym = 2-amino-4,6-dimethylpyrimidine, H(2)suc = succinic acid, H(2)bdc = 1,3-benzenedicarboxylic acid), were synthesized and characterized. The structure of 1 presents an argentophilic interaction enhanced 2-crossing [2]-catenane motif formed between pairs of parallel interpenetrating 2D 4(4)-sql nets. For 2, as the dicarboxylate changes from flexible H(2)suc to rigid V-shaped H(2)bdc, each highly undulated 2D 4(4)-sql net was penetrated in parallel by the two nearest neighbouring ones and thus the overall 2D -> 3D network is formed. The geometries and flexibility of dicarboxylates influence the coordination environments of metal ions and arrangement of ligands and thus determine the structures of the CPs. The photoluminescent and thermal properties of 1 and 2 have also been investigated.National Natural Science Foundation of China 20721001 MSTC 2007CB81530

Preparation and self-assembly of copper nanoparticles via discharge of copper rod electrodes in a surfactant solution: a combination of physical and chemical processes

地址: 1. Xiamen Univ, Dept Chem, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] nanoparticles with a mean diameter of 10-15 nm were prepared and self-assembled via discharge of bulk copper rods in a cetyltrimethylammonium bromide (CTAB)/ascorbic acid solution. Ascorbic acid was used as a protective agent to prevent the nascent Cu nanoparticles from oxidation in the solution;, otherwise spindle-like Cu2O/CuO structures, with a lateral dimension of 30-50nm and length of up to 100nm, were formed in pure deionized water. The surfactant CTAB had a critical influence on self-assembly of spherical Cu nanostructures (with diameter of 700 nm-1mum). Such a low-temperature and non-vacuum method, exhibiting the characters of both physical and chemical processes, provides a versatile choice for economical preparation and assembly of various metal nanostructures. (C) 2004 Elsevier Inc. All rights reserved

From Graphene Sheets to Boron Nitride Nanotubes via a Carbon-Thermal Substitution Reaction

通讯作者地址: Xie,SY(通讯作者),Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, 422 Si Ming Rd, Xiamen 361005, Peoples R ChinaNSFC,21031004,21021061,973 Projects 2007CB815301 2011CB93590

Determination of hydrogen peroxide in food

利用猪血红蛋白(PHb)的催化特性,以3,3',5,5'-四甲基联苯胺(TMb)为底物,建立了一种催化光度终止法测定食品中残留过氧化氢(H2O2)的方法。实验考察了终止剂的类型和浓度、反应时间、PH、温度、TMb浓度对H2O2检测的影响。在选定的反应条件下,体系吸光值与H2O2浓度在1.46x10-6~2.91x10-5MOl/l范围内呈良好的线性关系,线性相关系数为0.9958,方法对H2O2的检出限为5x10-7MOl/l,测定标准偏差为4.9%,样品的加标回收率为92.9%~103%,利用该方法进行了实际样品中的H2O2分析,结果与国标法无显著性差异。A new spectrophotometric method was developed for the determination of hydrogen peroxide(H2O2) in foods using 3,3′,5,5′-tetramethylbenzidine(TMB) as a terminating agent in the catalytic reaction between hemoglobin porcine(pHb) and H2O2.The influence of terminating agents,reaction time,pH,temperature and the concentration of TMB was discussed.Under the optimal experimental conditions,the absorbance value was linearly correlated to H2O2 concentration in the range of 1.46×10-6～2.91×10-5 mol/L with the correlation coefficient of 0.9958.The detection limit was found to be 5×10-7 mol/L and RSD was 4.9 %.The recoveries in the range of 92.9 %～103 % were obtained.The determination results corresponded well with those by the national standard method

一种碳纳米管阵列的生长方法

【申请号】CN200810071868.5【申请日】2008-09-26 【公开号】CN101372327【公开日】2009-02-25 【申请人】厦门大学【地址】361005福建省厦门市思明南路422号 【发明人】谢素原;马春印;林水潮;江智渊;黄荣彬;郑兰荪 【专利代理机构】厦门南强之路专利事务所【代理人】 马应森 【国省代码】35一种碳纳米管阵列的生长方法,涉及一种碳纳米管,尤其是涉及一种低能耗、无炉生长超长碳纳米管阵列的方法。提供一种碳纳米管阵列的生长方法。提供一基底,在该基底上表面依次溅射一层缓冲层和一层催化剂层,得上表面溅射有一层缓冲层和一层催化剂的基底；将上表面溅射有一层缓冲层和一层催化剂的基底放在加热片上；启动微区加热控制电源,直接以加热片为加热装置对基底加热；通入载气和碳源气；关闭微区加热控制器电源,将基底取出,得到样品

Al及Al合金粉末颗粒表面氧化膜厚度对爆炸烧结体质量的影响

本文借助光学显微镜、扫描电镜分析研究了水雾化、氮气雾化al粉和超声氩气雾化Al-Li合金粉爆炸烧结体的显微结构, 结果表明: 只有Al及Al合金粉的表面氧化膜厚度小于30nm时, 才可以得到性质优异的爆炸烧结体

重庆中坝遗址地层Na-Ca元素含量揭示的制盐业兴衰史

在对中坝和玉溪遗址考古断代和AMS14C测年基础上,根据对中坝遗址201个地层样品ICP等离子发射光谱的Na、Ca、Mg元素测定及玉溪遗址47个地层样品的Na、Ca元素测定,发现中坝制盐遗址地层中存在明显的Na、Ca元素含量反向相关期有35处之多,即:当Ca元素为峰值时,Na元素为谷值,反之亦然。其中,Ca元素为峰值、Na元素为谷值的反向相关期有21次,揭示出中坝遗址近5ka来制盐业有过近21个兴盛期。另有14个Na元素为峰值、Ca元素为谷值的反向相关期,揭示中坝遗址近5ka来制盐业也有过近14个衰落期。考古发掘得出的"中坝制盐业萌发于新石器时代、夏商发展、西周至汉代为鼎盛期、唐宋时代维持稳定发展、宋以后由于海盐进入川江地区导致中坝制盐业逐渐衰落、但20世纪70-80年代仍有生产"的结论证明本文Na、Ca元素含量反向相关性得出的研究结果是正确的。以上研究结果表明,中坝制盐遗址中Na、Ca元素含量有明显的反向相关规律,这一规律可以用于揭示盐业遗址的制盐业兴衰过程历史