Cell Micropatterning by photolithography or CO_2 Laser

细胞微图型化技术是一个多学科高度交叉的领域，它在许多方面有着重要的潜在应用：第一，可应用于大规模的药物发现与筛选；第二，是细胞传感器上用于固定细胞的一个重要手段；第三，是组织工程学的研究基础；第四，为分子细胞基础生物学的研究提供另外一种方法。构建细胞微图案也是我课题组进行细胞相关研究的基础。目前已经发展了许多种细胞微图案的制作方法。主要有光刻法、软光刻法（包括微印章技术及微流体技术）、“墨水”喷印技术、模板辅助图案技术、激光诱导直写技术、电化学图案化技术等。这些方法各有各的优缺点，但它们的制作均较为复杂，操作较为困难。本论文主要是运用光刻法及二氧化碳激光诱导法制作细胞微图案，主要的研究内容及获...Cell micropatterning is an interdisciplinary technology and has great potential applications on many fields. First, it is a valuable approach towards fundamental cell-biology studies. Second, it can be applied to the cell-based drug discovery and selection on large scale. Third, it is an important tool for organizing cells on transducers for cell-based sensing. The last one, the ability to produce...学位：理学硕士院系专业：化学化工学院化学系_物理化学(含化学物理)学号：20032505

CO_2-laser micromachining for production of PMMA-based microarrayer

提出利用CO2激光器快速制作微阵列点样芯片。通过测量激光在不同功率下加工小孔或是对同一小孔加工不同次数时所得小孔孔径和深度,以此来确定激光器的各项性能参数。并且测得不同功率的激光穿透小孔以及穿透次数不同所得小孔孔径。最后利用直径约为79μm的10×10阵列式孔道制作微阵列点样芯片,于尼龙膜上点样,所得样点平均直径为200μm,并测得小孔孔径与样点两者相对标准偏差分别为4.3%和7.4%。A new method to produce microarrayer rapidly by CO_2-laser system was presented. The system parameter was assessed of the depth and diameter of laser-ablated hole on the laser beam power and on the number of marks of the beam in the same hole. And the diameters of penetrated holes on the laser beam power and on the number of marks of the beam in the same hole were measured. The microarrayer was produced by the 10×10 array of holes. The diameters of holes were about 79μm. The diameters of spots which were printed on nylon membrane were about 200μm. And the CV of holes and the spots were 4.3% and 7.4%国家自然科学基金(No.20475047)资助项

An in-situ focusing electrophoresis concentration device for fluorescence detection

应用聚焦电泳原理,设计了一种用于荧光检测的现场浓缩装置。本装置是一种改进型的比色池,可作为荧光计的标准配件。以检测溴酚蓝、荧光素钠为例,装置的浓缩效率达6 7% ,使荧光强度增强32 .5倍。Based on the principle of focusing electrophoresis, an in-situ concentration device for fluorescence detection was developed. The principles and construction of the device are described. The device is actually an improved type of colour comparison cuvette. Taking bromphenol blue and Na-fluorexcein for examples, the concetration efficiency of the device is 67%, and the fluorescence intensity has increased 32.5 times. The device can be used as a standard equipment of fluorescence spectrophotometer

An Electro-driving High Efficiency,Continuous,No-damage Focusing Electrophoresis Concentration Device

提出使用膜聚焦浓缩方法并建立电驱动高倍率、连续、无损伤装置.分别以溴酚蓝、荧光素钠溶液为浓缩对象进行测试.该实验装置达近2个数量级浓缩效果,并可根据需要放大,成为有规模产量的生产设备,也可微型化,成为结构简单小巧、可以装配到电泳、色谱、质谱或光谱等分离分析仪器的零部件,或集成在芯片上,成为芯片实验室的预浓缩元件.We presented a membrane focusing concentration method and fabricatedan electro-driving high efficiency,continuous,no-damage focusing electrophoresis concentration device.In the concentration experiments of bromophenol blue and Na-fluorescein,the concentrated efficiencyof the device reached two orders of magnitude.This device can be magnified to industrial equipment with large output.It also can be miniaturized to small and simple attachment for separated and analytical instrument such as electrophoresis,chromatogram, mass spectrum and optical spectrum.When integrated into chips,it would be a pre-concentrated unit of the lab-on-chip.作者联系地址：厦门大学化学化工学院化学系固体表面物理化学国家重点实验室,厦门大学化学化工学院化学系固体表面物理化学国家重点实验室,厦门大学化学化工学院化学系固体表面物理化学国家重点实验室,厦门大学化学化工学院化学系固体表面物理化学国家重点实验室,厦门大学化学化工学院化学系固体表面物理化学国家重点实验室,厦门大学化学化工学院化学系固体表面物理化学国家重点实验室 福建厦门361005,福建厦门361005,福建厦门361005,福建厦门361005,福建厦门361005,福建厦门361005Author's Address: Department of Chemistry and State Key Laboratory for Physical Chemistry of Solid Surfaces,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,Fujian,Chin