尿细胞角蛋白19在膀胱移行细胞癌术后监测中的应用

目的　评价尿细胞角蛋白19片段(CK19,又称CYFRA2 1 1)在膀胱移行细胞癌(BTCC)术后监测中的作用。方法　对67例保留膀胱的BTCC术后患者定期行膀胱镜检查发现2 1例复发,采用电化学发光免疫分析(ECLIA)技术测定复发组(2 1例)、未复发组(4 6例)和正常对照组(2 4例)的尿CKl9含量。结果　复发组尿CKl9的平均值为(19.60±18.5 7) μg/L ,明显高于未复发组(2 .3 3±1.64 ) μg/L和正常对照组(1.82±0 .83 ) μg/L。复发组与未复发组及正常对照组比较,差异有统计学意义(P <0 .0 1)。尿CKl9对BTCC复发的敏感性为90 .5 % ,特异性为76.0 %。结论　尿CKl9是监测BTCC术后复发较为敏感、特异而且快速无创的指

Expression and Localization of hnRNP A2/B1 during Differentiation of Human Osteosarcoma MG-63 Cells Induced by HMBA

背景与目的:核基质蛋白的差异表达与细胞癌变和增殖分化调控关系密切。本研究观察了hnRNP A2/B1在诱导分化处理前后人成骨肉瘤MG-63细胞核基质中的存在和分布,及其与Actin、Prohibitin的共定位关系。方法:选择性抽提经环六亚甲基双乙酰胺(hexamethylene bisacetamide,HMBA)诱导处理前后的MG-63细胞核基质,并运用双向电泳、质谱分析、蛋白质印记杂交、免疫荧光、激光共聚焦等技术检测hnRNP A2/B1在核基质中的表达与定位变化,及其与相关蛋白的共定位关系。结果:双向电泳及蛋白质印迹杂交结果证实了hnRNP A2/B1存在于MG-63细胞核基质蛋白组分中,并在HMBA处理后细胞核基质中表达下调;免疫荧光显微镜观察显示hnRNP A2/B1定位在核基质上,经HMBA处理后hnRNP A2/B1表达减弱。激光共聚焦显微镜观察结果显示,hnRNP A2/B1与细胞核基质蛋白组分Actin、细胞增殖相关调控因子Prohibitin具有共定位关系,但在诱导处理后细胞内的共定位关系减弱。结论:hnRNP A2/B1在MG-63细胞诱导分化过程中的表达分布,及其与Actin、Prohibitin的共定位关系的改变对MG-63细胞分化具有重要影响,值得进一步探索和研究。BACKGROUND & OBJECTIVE: The differentially expressed nuclear matrix proteins have great effects on canceration and regulation of cell differentiation. This study was to explore the existence and distribution of ribonucleoprotein hnRNP A2/B1 in nuclear matrix and its co-localization with Actin and Prohibitin in human osteosarcoma MG-63 cells before and after hexamethylene bisacetamide (HMBA) treatment. METHODS: The nuclear matrix of MG-63 cells before and after treatment of HMBA were selectively extracted. The expression and localization of hnRNP A2/B1 in nuclear matrix were detected by 2-D PAGE, MALDI-TOF-MS, Western blot, and immunofluorescent staining. The co-localization of hnRNP A2/B1 with Actin and Prohibitin was observed under laser scanning confocal microscope (LSCM). RESULTS: hnRNP A2/B1 was detected in the component of nuclear matrix proteins of MG-63 cells by Western blot and immunogold staining and its expression was decreased after treatment of HMBA. hnRNP A2/B1 was located in the nuclear matrix, and its expression was weakened after HMBA treatment. hnRNP A2/B1 was co-localized with Actin or Prohibitin in MG-63 cells, while the co-localization relationship was weakened during differentiation of MG-63 cells. CONCLUSIONS: hnRNP A2/B1 is a kind of nuclear matrix protein, and localizes in the nuclear matrix. The distribution and expression of hnRNP A2/B1 and its co-localization with Actin and Prohibitin play important roles during the differentiation of MG-63 cells.国家自然科学基金项目(No.30470877)~

深度闭合性肾裂伤的非手术治疗

总结25例深度闭合性肾裂伤的救治经验。认为对深度闭合性肾裂伤,可根据具体情况,在严密监护下行非手术治疗,效果满意

纪念五四宪法颁布暨全国人民代表大会成立五十周年理论研讨会纪要

今年 9月是五四宪法颁布、全国人民代表大会成立五十周年。五四宪法是新中国的第一部宪法 ,为我国的社会主义民主法制奠定了基础 ,具有划时代的意义。坚持和完善人民代表大会制度 ,是新的历史条件下推进政治体制改革和社会主义政治文明建设的重要内容。基于“以学术表达纪念、以纪念促进学术”的目的 ,中国社会科学院法学研究所于 2 0 0 4年 9月 5日举办了“纪念五四宪法颁布暨全国人民代表大会成立五十周年理论研讨会”。来自全国各地的 70余位代表莅临会议 ,研讨了诸多热点和前沿问题

结构试验全方位通用加载系统研制与应用

近年来随着大跨空间和高层建筑的建设规模增大,使得目前的结构试验装置已经不能满足较高的试验要求。在总结国内外现有结构试验装置特点的基础上,研制了一种新型全方位通用加载系统(General Purpose Loading System,GPLS)。该球形系统由箱形圆弧梁焊接组装而成,可对空间尺度在6.1m内的任意形式构件或结构进行任意方位的静力单调或滞回加载,最大提供荷载达30 000kN。另外,为上半球反力架的提升和滑移操作设计了一套提升滑移装置。为了验证加载系统在极限试验荷载下的强度和刚度,采用有限元分析验证了反力架的强度和变形。最后介绍了采用该加载系统已经完成的系列大型空间复杂节点和子结构试验,典型试验结果表明加载系统具有优越的通用性和加载性能,能提高结构试验效率和减少试验成本