Research on the Construction of Public Interest Litigation Trust System in China

诉讼信托是兼具当事人和信托形式的一种制度，对公益诉讼领域的当事人适格扩张具有重要意义。我国尚未建立体系化的诉讼信托制度，仅在少数公益保护领域突破诉讼信托的禁止性规定。随着我国市场经济的迅猛发展，群体性、公益性的纠纷频繁发生，引起社会各界的广泛关注。公益诉讼信托制度具有独特的制度优势，将在纠纷解决中发挥重要的作用。本文通过界定诉讼信托及其相关制度的内涵，分析诉讼信托的理论基础，考察不同国家和地区对诉讼信托制度的态度，以寻求构建我国公益诉讼信托制度的具体路径，完善公益诉讼当事人制度。除引言和结语外，全文分为四章。 第一章辨析诉讼信托及与之相关的概念。第一节介绍诉讼信托的定义及特征，并引出公益诉讼...Litigation Trust is a kind of trust system as well as a special form of litigant, which is important to the expansion of standing in Public Interest Litigation area. There hasn’t established systematic Litigation Trust system in our country; it’s just applicable to part of Public Interest Litigation areas. With the rapid development of market economy in our country, the group disputes and public i...学位：法学硕士院系专业：法学院_诉讼法学学号：1362014115020

Identification of several fish eggs and larvae by DNA barcoding in Xiamen Water

以厦门海域采集到的鱼卵、仔稚鱼为研究对象。首先根据形态学特征将其分为2种类型鱼卵和3种类型仔稚鱼,然后应用dnA条形码技术分析鉴定得到3个鱼卵种类和4个仔稚鱼种类。其中,6种鉴定到种的水平,分别为汉氏棱鳀(THrySSA HAMIlTOnII)鱼卵、颈带鲾(nuCHEQuulA nuCHAlIS)鱼卵、纹缟鰕虎鱼(TrIdEnTIgEr TrIgOnOCEPHAluS)仔鱼、髭缟鰕虎鱼(TrIdEnTIgEr bArbATuS)仔鱼、斑鰶(kOnOSIruS PunCTATuS)仔鱼和鲬(PlATyCEPHAluS IndICuS)稚鱼;1种鉴定到属,即双边鱼属(AMbASSIS SP.)鱼卵。研究结果表明,将鱼卵、仔稚鱼鉴定到种的水平,绝大多数都需要借助于形态学之外的手段,尤其是形态特征表现相近的鱼卵。而dnA条形码技术采用自动化和标准化应用系统,能有效地对形态特征相似的鱼卵、仔稚鱼进行种类鉴定,具有准确性高、重复性好、人为误差少等优点,已成为传统形态学物种鉴定的强有力补充,在鱼类生物多样性研究中具有广阔的应用前景。This research focus on identification of fish eggs and larvae collected in Xiamen waters.The samples were distinguished to 2 types of fish eggs and 3 types of fish larvae by morphological characters and were identified as 3species fish eggs and 4 species fish larvae by DNA barcoding technology.In which,there were six species and one genus were identified,including Thryssa kammalensis egg,Nuchequula nuchalis egg,Tridentiger trigonocephalus larva,Tridentiger barbatus larva,Konosirus punctatus larva,Platycephalus indicus larva and Ambassis sp.egg.The results showed that molecular technology was necessary in identification of fish eggs and larvae to species,especially those species without enough morphological characters.By using automation and standardization system,DNA barcoding technology can identify the species of fish eggs and larvae effectively.This technology with the advantages of high reliability,excellent repetition and less error is a powerful complement for traditional identification by morphology and has wide application prospects in the study of ichthyoplankton diversity.福建省自然科学基金资助项目(2011J05116); 广西红树林保护与利用重点实验室系统性研究课题(GKLMC-201307

Isolation of Sperms,Eggs,Synergids and Central Cells of Carrot(Daucus carota L.)

通讯作者(E-mail: [email protected]; Tel: 0592-2186486)。[中文文摘]用两个解剖针挤压胡萝卜花粉使其破裂释放出精细胞。用酶解-解剖方法分离胡萝卜胚囊中的卵细胞、助细胞和中央细胞。胡萝卜胚珠先在酶液中酶解40~50min,然后将其转移到不含酶的分离液中用解剖针解剖胚珠。将胚珠的合点端切破,轻轻挤压胚珠的珠孔,卵细胞、助细胞和中央细胞即可逸出。在最佳条件下,20min可从20个胚珠中分离出5个卵细胞。对分离胚囊细胞的渗透压和酶液成分进行了筛选。分离出的卵细胞用显微操作仪收集。胡萝卜精、卵细胞的成功分离为在双子叶植物中进行离体受精探索创造了条件。[英文文摘]Carrot (Daucus carota L.) two sperms could be released by squeezing pollen grain using two dissecting needles. Viable egg cells, synergids and central cells of carrot were isolated using enzymatic digestion and mechanical dissection. The ovules were digested in enzymatic solution for 40–50 min, and then transported into the isolation solution without enzymes to dissect. When dissected ovules were sliced from their chalazal ends,eggs, synergids and central cells could be released by gently pushing ovule micropyle part. In optimal isolation condition, 5 egg cells could be isolated from 20 ovules during 20 min. The osmolality and enzymes in the enzymatic solutions were screened. The isolated egg cells could be collected using micromanipulator for preparation of molecular biology of egg cell of carrot. The isolation of egg cell of carrot will make a great chance for in vitro fertilization in a dicot plant.公益性行业(农业)科研专项经费(200903016);国家自然科学基金(31170289

化工实验教学体系模块化构建探索与实践

厦门大学化学工程与技术实验教学中心以培养化工专业创新性人才为目标,结合化工学科知识结构分布及实验教学的特点,对化工实验的教学内容、教学模式及师资队伍建设进行了改革,构建了"层次化、模块化"的教学体系,切实有效地提高了实验教学质量,以期培养新型工科人才。福建省本科高校教育教学改革研究项目(FBJG20170300

Characteristics of nitrogen and phosphorus losses from swine production systems in Jiulong River watershed

由于对畜禽粪便的处置和管理不当,氮、磷养分随粪便大量流失,不仅带来日益突出的环境问题,还造成了资源的浪费。目前国际上多采用养分平衡方法研究和评价畜牧养殖场养分流失状况及其潜在的环境风险程度。选取福建省九龙江流域92家养猪场,采用养分平衡分析方法研究了该流域养猪场养分流失状况及主要影响因素。结果表明:大部分养猪场的氮磷养分输入量远远大于养分有效输出量,表明养分大量流失,养殖系统的环境风险程度极高;随着养猪场规模的增大,氮、磷的不平衡程度呈下降趋势;生态型养殖模式有利于促进资源综合利用,减少养分流失;加强对粪肥和饲料管理有利于促进畜禽养殖场的氮、磷养分平衡。Huge losses of nitrogen and phosphorus from the livestock production systems are known as the waste of resources and the sources of the environmental quality problems. Nutrient balance at farm level is an important tool to study the environmental problems associated with livestock production. A nutrient balance was constructed for 92 swine production systems of Jiulong River watershed, Fujian Province. The majority of the farms exhibited substantially greater nutrient inputs than nutrient managed outputs and it was in the status of an overaccumulation of nutrients; the nutrient imbalance decreased with farm scales; ecological production was a preferred mode to make better use of resources and decrease the losses of nutrients; management of feed and manure was helpful to balance the nutrients of livestock farms.福建省重大科技项目(2002H009)资

Optimization of separation methods and culture system of chicken embryonic stem cells in vitro

背景:胚胎干细胞是从动物早期胚胎的内细胞团或原始生殖细胞分离出来的具有发育全能性的一种未分化的无限增殖细胞系。而鸡胚胎干细胞则是从X期鸡胚的胚盘分离而来。目的:优化鸡胚胎干细胞分离方法和离体培养体系。方法:采用滤纸纸环-发环的方法从X期鸡胚分离胚盘细胞,并采用STO细胞作为饲养层和大鼠肝细胞(brl)条件培养基(CM)+细胞因子作为离体培养体系对分离的胚盘细胞进行培养。结果与结论:滤纸纸环-发环法获得的完整胚盘率为75%~85%,克隆形成率约为50%。brl-CM+饲养层培养体系,鸡胚胎干细胞可传至7代,而brl-CM+饲养层+细胞因子培养体系,鸡胚胎干细胞可传至25代。分离到的鸡胚胎干细胞,经碱性磷酸酶染色、SSEA-1染色鉴定,表明鸡胚胎干细胞处于未分化状态。提示,实验不仅优化了鸡胚胎的分离方法,获得完整且杂质少的胚盘,而且进一步优化了鸡胚胎干细胞体外培养体系。BACKGROUND:Embryonic stem cells are undifferentiated permanent cell line derived from inner cell mass cells and primordial germ cells of animal's early embryos.Chicken embryonic stem cells are derived from the blastodermal of a X-stage embryo.OBJECTIVE:To optim the separation method and in vitro cultural system of chicken embryonic stem cells.METHODS:The X-stage chicken embryos were isolated by using a small square of ?lter paper with a hole punched in the center,and the blastodermal cells were isolated by using the hair loop.STO cells were used to make feeder layer;at the same time,BRL-CM and cytokine were also used for chicken embryonic stem cells in vitro cultural system.RESULTS AND CONCLUSION:The filter paper loop and the hair loop could obtain complete the blastoderm,and the successful percentage was 75%-85%.The colony formation rate was about 50%.After culture in the BRL-CM + feeder layer + cytokine culture system,the passage of CES cells is the seventh generation;BRL-CM + feeder layer + cytokines,cultured chicken embryonic stem cells could passage to the 25th generation.Isolated chicken embryonic stem cells were in an undifferentiated state detected by alkaline phosphatase staining and SSEA-1 staining.The findings indicate that this experiment not only optimized the isolation method of chicken embryonic stem cells to obtain complete and pure embryos,but also further improved the in vitro culture system of chicken embryonic stem cells.国家973项目(2009CB941600)资助;国家自然科学基金项目(31072101)资助---

学校流感暴发疫情防控措施动力学模型效果分析

目的探讨学校流感暴发疫情的最佳控制方式,为制定停课标准提供参考。方法通过SEIAR模型对流感暴发疫情隔离和停课效果进行分析。结果本起流感暴发疫情基本再生数R0为7.6180,无干预措施下疫情会持续20天,1 169名学生和教职工（99.74%）会感染流感。第5～10天开始隔离措施,疫情持续时间介于35～44天,第10天开始隔离感染人数是第5天开始隔离的6.9倍。与单独采取隔离措施相比,停课1天、3天、5天、7天可以减少4.51%～10.92%、13.41%～29.31%、19.46%～45.40%和24.82%～57.47%的感染人数,持续时间并没有太大变化;停课14天疫情持续时间都会明显下降,感染人数下降33.43%～65.52%。结论隔离措施越早实施对疫情控制效果越好,显性感染人数达到20%以上时可以停课,出现重症或死亡病例时要将停课时间延长

The Difference of Luminous Performance Between Traditional Phosphor Packaging LED and Remote Phosphor LED

研究了传统白光lEd与蓝光激发的球冠形远程荧光粉白光lEd在不同电流、不同热沉温度下的发光性能,并对其机理差异展开了探讨。实验结果表明:随热沉温度和驱动电流的上升,传统白光lEd的量子效率和电光转换效率急剧下降,并导致其y/b比(yEllOW/bluE rATIO)下降,相关色温上升。而在远程荧光粉白光lEd中,其量子效率、光转换效率和相关色温在相同实验条件下变化幅度都较小。由光强空间分布和y/b比空间分布可知,远程荧光粉白光lEd的光强分布呈类似蝠翼分布,且y/b比空间均匀性远大于传统白光lEd。Under different drive current and heat sink temperature,luminous performances as well as physical mechanisms were studied for both traditional phosphor-dispensing packaging white LED and blue light-converting hemisphere remote phosphor LEDs.With the increase of the heat sink temperature and the drive currents,the quantum efficiency and light conversion efficiency in the traditional phosphor-dispensing packaging white LED drop rapidly,which is responsible for the decrease of Y/B ratio(Yellow/Blue Ratio) and the apparent increase of CCT(correlated color temperature).However,the parameters of quantum efficiency,light conversion efficiency and CCT in blue light-converting hemisphere remote-phosphor packaging LEDs have little change under the same experimental conditions.In addition,the remote phosphor LEDs exhibit a batwing spatial luminous intensity distribution and the spatial Y/B ratio distribution is much more homogeneous compared with the traditional phosphor-dispensing packaging white LED.国家自然科学基金(11104230;61102030); 福建省产学研重大科技项目(2011H6025;2013H6024); 福建省重点科技项目(2012H0039)资

Proteomic Analysis of Rice Cultivar Jiafuzhan in the Responses to Xanthomonas campestris pv.oryzicola Infection

作者简介: 陈芳育(1978-) , 男, 讲师。E-mail : cfy307@ sohu. com * 通讯作者(Corresponding author) : 陈亮( 1963-) , 男, 教授, 博士生导师, 研究方向: 细胞与分子生物学。E-mail: chenlg@ xmu. edu. cn[中文文摘]运用双向电泳分析高抗水稻品种“佳辐占”受强毒力细菌性条斑病病原菌侵染2d后的叶片蛋白质组变化,共发现38个蛋白质发生差异表达,其中32个上调,5个下调,1个新增。用MALDI-TOF-MS分析和数据库检索鉴定出其中的33个差异表达蛋白质,并将它们分为4个功能类群,即信号转导相关蛋白、防卫相关蛋白、代谢相关蛋白和蛋白质稳定相关蛋白。这些蛋白分别参与了信号识别、信号传递、抗氧化、糖代谢、细胞壁加固、植保素合成等抗病生理反应。研究表明,水稻对细菌性条斑病病原菌的侵染存在着一个复杂的抗病信号应答和代谢调控网络,其作用机理可以通过差异表达的蛋白质(酶)反映出来,其中差异表达的8个R蛋白和3个PR蛋白可能与水稻对细菌性条斑病的抗病性密切相关。本研究为进一步揭示水稻对细菌性条斑病的抗性机理及相关抗病基因的功能克隆提供了依据。[英文文摘]Rice bacterial leaf streak( BLS) caused by the pathogen Xanthomonas oryzae pv. oryzicola ( Xooc) is one of the major rice diseases in South China. Here we focus on proteomics as a tool for the discovery of differentially expressed proteins closely related to the disease resistance. The leaves of rice cultivar Jiafuzhan (Oryzae sativa L. ) highly resistant to the disease, were infected by"89773-1- 1" strain of the Xooc with strong pathogenicity. Total proteins were extracted from the leaves sampled at two days after inoculation, and separated by two- dimensional electrophoresis. It was found that there were thirty- eight proteins expressed differentially, of which thirty-two were up-regulated, five down-regulated and one was "new". Of the thirty- eight responsive proteins, thirty-three were identified by MALD-I TOF-MS and database searching.Based on the predicted function, we grouped them into four clusters: signal transduction, defensive responses, substance metabolism and protein stabilization, which were involved in many resistant physiological react ions, including signal recognition and transduction, antioxidant react ion, carbonhydrate metabolism, cel-l wall reinforcement and phytoalexin biosythesis. In turn a complex signal transduct ion and metabolic regulative network in the resistant responses to the infection of Xooc was outlined in this work, and the molecular mechanism was revealed by differentially expressed protein/enzyme patterns during Xooc infection. In this study, eight R proteins and three pathogenesis- related(PR) proteins which might relate closely to the disease-resistance were found. This result provides us the basic information to further reveal the resistant mechanism and conduct functional cloning of the resistan-t related genes in rice to BLS.生物农药与化学生物学教育部重点实验室( 福建农林大学) 开放课题基金项目( KF0411