Preliminary pharmacodynamic study of STS as an adjuvant ofthe chemotherapy drugs in Vitro and in Vivo

目的：研究硫代硫酸钠（STS）是否能在体内、外作为化疗药的辅药。方法：应用MTT法研究STS分别与AdM、MMC和CddP等6种抗癌单药合用时对bEl-7402和MgC80-3细胞的细胞毒性作用；应用小鼠肝癌腹水瘤（H22）模型判断STS与AdM等3种药合用时的抗癌疗效；20例人原发性肝癌病人肝动脉插管化疗（AdM、MMC和CddP）前30MIn静脉推注STS（125~25g/M2）考察STS作为化疗药辅药的作用。结果：除了CddP以外，STS（500μg/Ml）与AdM等5种抗癌单药合用时对抗癌药的抗肿瘤细胞活性无明显影响（P＞005）。当不同剂量STS（350Mg/kg、35Mg/kg）分别与AdM（6Mg/kg）、MMC（14Mg/kg）和CddP（45Mg/kg）合用或仅这3种化疗药合用时，这3组腹水瘤小鼠的存活期比对照组者显著延长（P＜0001），但3组之间无明显区别（P＞005）。人肝癌肝动脉插管化疗时，STS与AdM、MMC和CddP抗癌药合用，肝癌治疗总有效率达60%，且可减少70%病人的恶心、呕吐等副作用。结论：在体内，低浓度的STS（125~25g/M2）可与AdM，MM？Objective: To study whether sodium thiosulfate (STS) can be used as an adjuvant of the chemotherapy drugs. Methods: The cytotoxicity of STS combined with ADM, MMC, CDDP, 5Fu, MTX and VCR (1PPC/ml) respectively on the cells of BEL7402 and MGc803 was studied by MTT test in vitro.The ascitic hepatoma (H22) in mice were adopted to determine the anticancer effect of STS adjuvant of ADMMMC and CDDP.Being treated with STS(12525 g/m2) 30 min before drug administration,20 patients of hepatocellular carcinoma (HCC) were carried on transcatheter arterial chemoembolization with ADMMMC and CDDP to observe the adjuvant effect of STS. Results: The anticancer activity of ADM and the other agents respectively adjuvant with STS (500 g/ml) were not obviously influented (P >005), but that for CDDP was influented .It was proven that the survival time of ascitic hepatoma treated with ADM (6 mg/kg),MMC (14 mg/kg) and CDDP (45 mg/kg) alone or three drugs in addition to STS (350 mg/kg,35 mg/kg) respectively was not significantly different (P >005).The survival time of each groups was significantly longer than that of control group (P< 0001).It have also been proven that STS adjuvant of ADM, MMC and CDDP was not decreased the chemotherapry effect (RR=60%) by transcatheter arterial chemoembolization in HCC, simultaneously it alleviated the nausea and vomiting in 70%patients. Conclusion: The study provides evidence that in vivo, STS (12525 g/m2) can be used as an adjuvant of chemotherapy drugs,such as ADM,MMC, including CDDP, and so on , for decreasing nausea and vomiting effects, espacially for transcatheter chemotherapy .福建省“八五”肝癌攻关课

Sarcomatoid Carcinoma of Hypopharynx： A Report of 5 Cases

目的探讨下咽肉瘤样癌的临床及病理学特点,为下咽肉瘤样癌的诊治提供参考依据,提高对肉瘤样癌的诊断、治疗及其预后的认识。方法分析2010年1月—2014年9月我院治疗的5例下咽肉瘤样癌患者的临床资料,包括诊疗过程、病理特征等。结果 5例患者均经过不同的手术方式,其中咽后壁扩大切除1例,梨状窝扩大切除2例,全后、全下咽、全食管切除2例;肉瘤样癌肉瘤样成分与癌成分共存,且之间有一定的移行,CK、vimentin均表达阳性,P63阳性4例。5例患者均定期随访。结论下咽肉瘤样癌有其特殊的生物学行为及病理学特点,是一种不同于其他类型的恶性肿瘤,具有自身病理特征,预后情况各不相同,外科手术仍是首选治疗方法,术后辅助放化疗。Objective To explore the biological behavior and clinical features of the sarcomatoid carcinoma（SC） of hypopharynx for improving the diagnosis,treatment and judgment of prognosis of the tumor.Methods Clinical data,including treatment process and pathology features of the 5 cases of SC underwent operation in our department from January 2010 to September 2014 was analyzed retrospectively.Results All the five patients underwent different surgical procedures and all survived,one case received pharyngeal extended resection,tow cases received pyriform extended resection and tow cases received total throat、swallow、esophagectomy resection.The biological of sarcomatoid carcinoma of head and neck performed components and cancer components coexisted,and has a certain shift between them,CK and vimentin expressed was positive in all cases,P63 was positive in 4 cases.All patients were followed up regularly.Conclusion Sarcomatoid carcinoma of hypopharynx has its special biological behavior and pathology characteristics,it is different from other types of malignant tumors,and has its own pathological features,surgeryis is still the preferred method,postoperative assistance chemotherapy is needed

Research on T Lymphocyte Subsets and NK Cells Activity in Peripheral Blood of Hypopharyngeal Squamous Cell Carcinoma

目的:分析下咽鳞状细胞癌患者外周血T淋巴细胞亚群和NK细胞活性情况,旨在为相关研究工作提供参考资料。方法:择取2011年6月-2016年5月本院收治的95例下咽鳞状细胞癌病患为病例组,另取此期间内来本院接受健康检查的72例志愿者为正常组。使用流式细胞仪对病患开展相关检查,分析病例组与正常组的淋巴细胞亚群、NK活性情况以及不同临床分期的下咽癌病患外周血T淋巴细胞亚群以及NK细胞活性之间的关系。结果:与正常组相比,病例组的NK细胞、总B淋巴细胞、总T淋巴细胞、辅助/抑制T细胞、辅助T细胞偏低(P0.05)。Ⅰ~Ⅱ期病患辅助T淋巴细胞比Ⅲ~Ⅳ期者略高(P>0.05)。Ⅰ~Ⅱ期病患抑制T淋巴细胞值比Ⅲ~Ⅳ期者低(P0.05)。Ⅰ~Ⅱ期病患外周血NK细胞数比Ⅲ~Ⅳ期者略高(P>0.05)。结论:下咽鳞状细胞癌病患的免疫功能异常,且随着疾病的进展,细胞免疫和体液免疫均有所降低,证实免疫功能紊乱在疾病进展中发挥了一定作用。经流式细胞设备对外周静脉血淋巴细胞亚群以及NK细胞活性情况检测,可实现动态化检测癌症病患某阶段细胞免疫功能变化详情,此法方便简单,在指导治疗方案制定以及监测疾病预后方面,均体现出了一定效果,值得进一步推广

动脉粥样硬化性肾动脉狭窄的早期发现

探讨冠状动脉造影同时行肾动脉造影的必要性及动脉粥样硬化性肾动脉狭窄的相关危险因素分析。共 4 91例临床疑似冠心病患者行冠状动脉造影同时行非选择性或选择性肾动脉造影 ,并对相关的临床因素进行评价 ,筛选出动脉粥样硬化性肾动脉狭窄的独立危险因素。冠心病组患者中动脉粥样硬化性肾动脉狭窄的发病率为2 0 % ,显著高于非冠心病组 (2 .6 % )。冠心病、外周血管疾病是动脉粥样硬化性肾动脉狭窄的独立危险因素 (多元Logistic回归分析示 ,P <0 .0 0 1、P =0 .0 0 3) ,是其早期发现的指标。对怀疑有冠心病的患者 ,在冠状动脉造影同时行肾动脉造影有助于动脉粥样硬化性肾动脉狭窄的早期发现

国产Amplatzer封堵器治疗先心病的初步临床研究

目的:报道用国产Amplatzer 封堵器治疗先天性心脏病(先心病)的临床经验。方法:82例先心病患者经超声和心血管造影诊断后,分别应用3种类型的国产Amplatzer 封堵器封堵动脉导管未闭(PDA)、房间隔缺损(ASD)和室间隔缺损(VSD),观察其安全性、术中和术后近期疗效。结果:PDA28例(漏斗型23例,管型5例), 直径4～11 mm,封堵成功率100%。继发孔型ASD34例,直径8～32 mm,封堵成功率97%。VSD20例,均为膜周部,直径3～10 mm,封堵成功率100%,术后即刻造影均成功,无明显并发症。结论:应用国产Amplatzer 封堵器治疗先心病是安全、有效的

Relieving EFFect of Sodium ThiosulFate on Transarterial Chemotherapic Emeses

报告硫代硫酸钠（STS）对20例肝动脉导管介入化疗病人的止呕辅助作用。通过体外琥珀酸脱氢酶抑制法（MTT）研究STS（500μg/Ml）辅助阿霉素（AdM）、丝裂霉素C（MMC）、顺铂（CddP）等7种抗癌药的抗肿瘤敏感试验，STS辅佐抗癌药的抗肿瘤活性无明显降低（P＞0·05），CddP除外。临床上，对肝癌病人经皮肝动脉插管化疗前30MIn静脉注射STS（1.25~2.50g/M2）或肌内注射灭吐灵（0.4Mg/kg）进行比较，观察两组病人呕吐的程度并进行统计分析。结果表明：STS比灭吐灵更能降低呕吐发生率而不影HCC的化疗效果。值得进一步研究STS辅佐其它化疗药的抗肿瘤作用，以及它在肿瘤化疗中的应用价值。It was reported that sodium thiosulFate (STS) was contributed to antivomiting eFFect in 20 transarterial chemotherapic patients.The antitumor sensitivity of STS (0.05 ) except For CDDP clinically,to comparing the adjuncting eFFects of STS (iv.30min ahead) or metoclopramdum (im.30min ahead) to ADM、MMC and CDDP on HCC(40 cases),the degrees of vomiting in hepatoma patient aFter transcatheter arterial chemoem bolization with ADM、MMC and CDDP were statisticaly analysed.It have been proven that STS was contributed to the low incidence of vomiting and superior to metocloe pramidum,without worsening of the chemotherapry of HCC.It is worth Futher studying adjuvant STS to other antitumor drungs and exploring potential application of chematherapy in cancer

One stage surgical treatment of multiple primary carcinoma of hypopharynx and esophagus

目的探讨下咽、颈胸段食管多原发癌(multiple primary carcinoma,; MPC)在胸腔镜辅助下行全喉、下咽、食管切除并管状胃重建一期手术的应用及疗效。方法胸科组行胸腔镜辅助下分离食管及纵膈淋巴结清扫后开腹行管状胃成形; ,头颈组行颈部淋巴结清扫、全喉下咽切除、咽胃吻合术。术后常规补充放化疗。结果本组全部病例均一期完成手术,肺部感染3例,胸腔积液2例、气管撕裂1例; ;无吻合口瘘及围手术期死亡病例;3年生存率63.6%,5年生存率50.0%。结论下咽癌应常规行胃镜检查以免MPC的漏诊;胸腔镜辅助下全喉、下咽、; 食管切除并管状胃重建术可一期完成以往分次手术难以完成的手术治疗,有效提高下咽颈胸段食管多重癌的治疗效果。OBJECTIVE To discuss the therapeutic effect of one stage surgical; treatment in the multiple primary hypopharyngeal and cervical thoracic; esophageal carcinoma. METHODS The thoracoscopy group: dissecting the; esophagus and mediastinal lymph node assisted with thoracoscope, and; then opened abdominal cavity to make gastric tube. Head and neck group:; doing the cervical lymph node dissection, total laryngectomy, total; hypopharyngectomy and total esophagectomy, and then anastomosis of the; pharynx with gastric tube. All cases were received conventional; radiotherapy and chemotherapy after operation. RESULTS All the cases in; this group were successfully underwent the one stage operation. The; postoperative complications were pulmonary infection in 3 cases, pleural; effusion in 2 cases and tracheal tear in one case. No anastomotic; fistula or postoperative deaths occurred. The 3 and 5 year survival; rates were 63.6% and 50.0% respectively. CONCLUSION It should take; necessary examinations of cervical thoracic esophagus to prevent missing; the multiple primary carcinoma of the hypopharyngeal carcinoma. The; total laryngectomy, total hypopharyngectomy and total esophagectomy, and; anastomosis of the pharynx with gastric tube for multiple primary; hypopharyngeal and cervical thoracic esophageal carcinoma is a feasible; and active treatment method