Construction of expression vector of HCV NS4B and its effect on cell apoptosis

目的 构建丙型肝炎病毒（HCV）2a型非结构蛋白4B（NS4B）的真核表达载体,并观察其对骨肉瘤细胞U2OS凋亡的影响。方法 以质粒PJFH1为模板,通过PCR反应扩增4目的基因片段,采用同源重组法与PCMV-tag2b载体相连,转化大肠杆菌感受态DH5α,筛选正确的克隆。以脂质体为介导转染U2OS细胞,通过Western blot检测NS4B蛋白的表达,免疫荧光检测细胞的凋亡。结果 构建pCMV-tag2b-NS4B重组质粒,经测序其与NCBI公布的序列完全一致,并可在U2OS细胞中表达,DAPI检测显示细胞凋亡率为（17.25±2.95）%,对照组凋亡率为（6.53±2.36）%。结论 成功构建NS4B的真核表达载体,并可在U2OS细胞中表达,诱导细胞凋亡。山西省自然科学基金（No：2014021037-9）; 山西省高等学校大学生创新创业训练项目（No：2014052）; 山西医科大学汾阳学院科技发展基金（No：2016B02

Identifying the Conformational Isomers of Single-Molecule Cyclohexane at Room Temperature

构象异构是化学中的基本问题。然而对于环己烷等柔性分子，由于其在室温下极快的互变异构过程，基于系综的表征方法（如核磁等）只能得到所有构象平均贡献的结果。为了应对这一挑战，化学化工学院洪文晶教授与夏海平教授课题组为在室温条件下对柔性分子构象的定量分析与表征这一挑战，课题组成功实现了在室温条件下对环己烷两种椅式构象的电学表征与比例识别。同时，通过纳米电极间隙对分子的限域作用，发现在宏观尺度下极不稳定的扭船式中间体得以在单分子尺度稳定存在，这为不稳定中间体的研究提供了重要表征方法。 这一研究工作是在化学化工学院洪文晶教授、夏海平教授共同指导下完成的，iChEM直博生唐淳与化工系研究生唐永翔为论文共同第一作者。师佳副教授与刘俊扬副研究员为该工作提供了指导，博士后陈志昕、博士研究生陈李珏以及研究生叶艺玲、严哲玮、张珑漪共同参与了该工作。【Abstract】Isomerism reflects the ubiquitous nature that molecules with the same molecular formula show different structures. The interconversion between conformational isomers of flexible molecules is quite fast owing to the low barriers of around 10 kcal mol−1, leading to average signal contributed by all the possible isomers characterized by ensemble methods. On this account, identifying the conformational isomers of flexible molecules at room temperature has a substantial challenge. Here, we develop a single-molecule approach to identify the conformational isomers of cyclohexane at room temperature through the single-molecule electrical characterization. By noise analysis and feature extraction of the conductance of single-molecule junctions, we quantificationally identified two chair isomers of cyclohexane at room temperature, while such identification is only feasible at low temperatures by ensemble characterization. The strategy to apply the single-molecule approach to identify conformational isomers paves the avenue to investigate the isomerization of flexible molecules beyond the ensemble methods.This work was supported by the National Natural Science Foundation of China (nos, 21722305, 21673195, 21703188, and U1705254), the National Key R&D Program of China (2017YFA0204902), China Postdoctoral Science Foundation (no. 2017M622060), and the Fundamental Research Funds for Xiamen University (20720190002).该工作获得了科技部国家重点研发计划、国家自然科学基金等项目的资助，也得到了固体表面物理化学国家重点实验室、能源材料化学协同创新中心的支持

Constraint of a ruthenium-carbon triple bond to a five-membered ring

含过渡金属碳三键(M≡C)的金属卡拜化合物是许多有机反应的催化剂或关键中间体。对其合成及性质的研究是金属有机化学的热点之一。由于卡拜碳的sp杂化方式，大部分金属卡拜化合物均为链状结构（卡拜碳键角理想值为180 °），环内金属卡拜化合物因存在很大的环张力而难于合成。夏海平教授课题组发展了由链状多炔（称之为碳龙）构筑碳龙配合物的高效方法（Nature Communications, 2017, 8, 1912），实现了锇杂戊搭炔/烯及其衍生物的一锅法合成。现在，他们把该方法进一步拓展到了第二过渡系金属钌，通过碳龙与市售的RuCl2(PPh3)3反应一锅合成了钌杂戊搭炔I。本研究是碳龙化学的进一步延伸和发展，首次把碳龙化学的金属中心由锇拓展到了其它金属，展示了碳龙化学广阔的发展空间。该研究工作在夏海平教授指导下完成，能源材料化学协同创新中心（iChEM）博士后卓庆德和张弘副教授为共同第一作者。iChEM博士后周小茜、博士生陈志昕、林剑锋、卓凯玥、硕士康慧君、林鑫磊参与了部分实验工作。博士生华煜晖负责理论计算。【Abstract】The incorporation of a metal-carbon triple bond into a ring system is challenging because of the linear nature of triple bonds. To date, the synthesis of these complexes has been limited to those containing third-row transition metal centers, namely, osmium and rhenium. We report the synthesis and full characterization of the first cyclic metal carbyne complex with a second-row transition metal center, ruthenapentalyne. It shows a bond angle of 130.2(3)° around the sp-hybridized carbyne carbon, which represents the recorded smallest angle of second-row transition metal carbyne complexes, as it deviates nearly 50° from the original angle (180°). Density functional theory calculations suggest that the inherent aromatic nature of these metallacycles with bent Ru≡C–C moieties enhances their stability. Reactivity studies showed striking observations, such as ambiphilic reactivity, a metal-carbon triple bond shift, and a [2 + 2] cycloaddition reaction with alkyne and cascade cyclization reactions with ambident nucleophiles.This research was supported by the National Key R&D Program of China (2017YFA0204902) and the National Natural Science Foundation of China (nos. 21490573,21332002, and 21561162001). 研究工作得到国家自然科学基金项目（21490573、21332002 和21561162001）和国家重点研发计划（2017YFA0204902）的资助

Successive modification of polydentate complexes gives access to planar carbon-and nitrogen-based ligands

以碳和氮为键合原子的多齿螯合物是配合物家族中非常重要的一类。具有更高齿数的平面构型NC螯合物实例相对较少，代表性的例子为四齿金属碳卟啉类化合物，这类螯合物以其独特的结构和丰富的物理化学性质引起广泛关注。然而平面五齿、六齿的NC螯合物由于几何构型“拥挤”导致合成难度高，该工作从含三元环内金属卡宾结构的CCCC型碳龙配合物出发，利用经典有机反应（炔烃对金属卡宾的插入反应），成功地实现了CCCCN/NCCCN型平面五齿螯合物的合成。这一研究为高配位型螯合物的合成提供了新思路并为平面五齿螯合物家族添加新成员。特别是，这些高配位型螯合物在可见光和近红外区域均有较好的吸收，表现出良好的光声成像、光热转换及声动力学性能。 该研究工作在张弘教授指导下完成，第一作者为iChEM博士后周小茜。该工作充分体现了多学科协同研究优势：相关化合物的合成、表征及理论计算工作由周小茜博士完成；声动力学性能研究由厦门大学公共卫生学院庞鑫博士及刘刚教授完成；光声成像研究由厦门大学公共卫生学院聂立铭教授完成。iChEM fellow卓庆德博士、博士生卓凯玥、陈志昕参与了部分实验工作。夏海平教授、香港科技大学林振阳教授和南京大学朱从青教授对研究工作给予了大力支持。【Abstract】Polydentate complexes containing combinations of nitrogen and carbon (N and C) ligating atoms are among the most fundamental and ubiquitous molecules in coordination chemistry, yet the formation of such complexes with planar high-coordinate N/C sites remains challenging. Herein, we demonstrate an efficient route to access related complexes with tetradentate CCCN and pentadentate CCCCN and NCCCN cores by successive modification of the coordinating atoms in complexes with a CCCC core. Combined experimental and computational studies reveal that the rich reactivity of metal-carbon bonds and the inherent aromaticity of the metallacyclic skeletons play key roles in these transformations. This strategy addresses the paucity of synthetic approaches to mixed N/C planar pentadentate chelating species and provides valuable insights into the synthesis of carbon-based high-coordinate complexes. Furthermore, the resulting complexes are the examples of organometallic species with combined photoacoustic, photothermal, and sonodynamic properties, which makes them promising for application in related areas.This research was supported by the National Natural Science Foundation of China (Nos. 21572185, 21561162001, and 81571744), the Research Grants Council of Hong Kong (N_HKUST603/15), the Excellent Youth Foundation of Fujian Scientific Committee (2018J06024), and the Fundamental Research Funds for the Central Universities (20720170065).该工作得到国家自然科学基金委、香港研究资助局、福建省自然科学基金、厦门大学校长基金的资助

Assessment of Genetic Diversity of Garlic(Allium sativum L.) by the Technique of Molecular Marker

本研究利用RAPD和ISSR两种分子标记技术,对中国10个不同地区的大蒜品种进行了种质资源遗传多样性研究.从30个RAPD引物当中,筛选出11个具有多态性的引物,共扩增出多态性带224条,多态性条带比率为41.18%,从12个ISSR引物中筛选出5个具多态性的ISSR引物,共扩增出多态性带121条,多态性条带比率为50.21%.对RAPD和ISSR两种标记分别运用Nei指数法,计算出10个大蒜品种的平均遗传距离为0.28和0.32.根据这两种标记的结果,采用UPGMA进行聚类分析,得到与生物学分类地位基本一致的结果.结果表明,在实验稳定性上,ISSR优于RAPD,且ISSR比RAPD能检测到更多的遗传变异,RAPD和ISSR两种标记可用于大蒜种质遗传多样性的研究. 【英文摘要】 Random amplified polymorphic DNA(RAPD) and inter-simple sequence repeat (ISSR) methods were used to detect genetic variation of 10 varieties of garlic (Allium sativum L.)from different regions and provinces of China.11 RAPD primers generated 224 polymorphic bands.Percentage of polymorphic bands (PPB) of RAPD was 41.18%.5 ISSR primers generated 121 polymorphic bands.PPB of ISSR was 50.21%.The result of UPGMA cluster analysis based on two molecular markers data was similar to the result based on the biologic ...教育部科技重点项目(104105);; 福建省青年科技项目(2001J033)资

颞下颌关节盘不可复性前移位关节内压测量与分析

【目的】了解关节盘不可复性前移位患者关节上腔中压力变化的规律, 初步探讨其对颞下颌关节紊乱病的分类 的指导意义。【方法】收集颞下颌关节盘不可复性前移位患者16 例22 侧, 用关节内压测量仪通过穿刺针进入关节上腔, 分别 测量患者关节张闭口位的关节上腔内压, 并记录波形和平均值, 测压后进行颞下颌关节镜手术。【结果】关节盘不可复性前移 位关节上腔内压可分3 种类型。高压型:压力普遍升高且波动幅度增大, 开口位平均压力为(-948 ±2 382)Pa , 闭口位压力 为(1 286 ±937)Pa ;反压型:压力变化规律反常, 开口位(997 ±1 132)Pa , 闭口位(-521 ±833)Pa ;低压型:关节上腔异常负 压, 开口位(-15 261 ±12 211)Pa , 闭口位(-8 163 ±3 203)Pa 。【结论】本实验测量了不可复性盘前移位关节开闭口位腔内 压, 并根据关节内压变化规律将关节盘不可复性盘前移位初步分为3 种类型, 结合关节镜所见从病理学角度提出了各种类型 的成因

EFFECT OF ZINC,SELENIUM AND MANGANESE ON THE GROWTH OF A MARINE MICROALGA NANNOCHLOROPSIS OCULATA(EUSTIGMATOPHYCEAE

正> The effect of trace metals of Zinc (Zn), Selenium (Se) and Manganese (Mn) on thegrowth of a marine microalga was determined by culturing Nannochloropsis oculata in f/2 medium enrichedwith different concentrations of the metal compounds. The results showed that concentration and type ofthe metal compound affected the growth of Nannochloropsis. ZnCl_2 and Na_2SeO_3, promoted the growth ofNannochloropsis at relatively low concentrations of 15. 3 μmol/L and 57. 8 μmol/L respectively, but inhib-it its growth... 【英文摘要】 The effect of trace metals of Zinc (Zn), Selenium (Se) and Manganese (Mn) on the growth of a marine microalga was determined by culturing Nannochloropsis oculata in f/2 medium enriched with different concentrations of the metal compounds. The results showed that concentration and type of the metal compound affected the growth of Nannochloropsis . ZnCl2 and Na, SeO3 promoted the growth of Nannochloropsis at relatively low concentrations of 15 .3 μmol/L and 57 .8 μmol/L respectively, but inhibit its growth at...This work was supported by Key Science Project of Fujian Province and by the Ministry of Education Foundation for University Key Teacher

互惠共生微生物多样性研究概况

所谓互惠共生微生物(mutualistic symbiotic microbes,MSM)是指能定殖其他生物构建互惠共生体系的微生物,主要包括互惠共生细菌、互惠共生放线菌和互惠共生真菌等。MSM种类繁多、分布广泛、物种多样性丰富,涉及原核生物界和真菌界等。MSM定殖人体、动物、植物、藻类或其他真菌,可构建各自相应的互惠共生体系,进而形成范围更加巨大的共生网络,发挥不可替代的生理生态功能。本文在介绍MSM概念的基础上,重点总结了MSM多样性研究进展,指出了目前研究中尚存在的问题,探讨了今后应该开展的工作,MSM多样性研究成果可望为研发MSM应用技术提供依据和材料

Research advances in mutualistic symbiotic microbes diversities

The so-called mutualistic symbiotic microbes (MSM) can colonize other organisms to build a mutual symbiotic system. MSM mainly include mutualistic symbiotic bacteria, mutualistic symbiotic actinomycetes and mutualistic symbiotic fungi. MSM are diverse, with a wide distribution and rich in species diversity, involving those in procaryotae and kingdom fungi. MSM colonize human body, animals, plants, algae, or other fungi, and can build their respective reciprocal symbiotic systems, and then form a wider symbiotic network to play irreplaceable physiological and ecological functions. On the basis of introducing the concept of MSM, this paper summarizes the progress of MSM diversity research, problems existing in the current research, and the work in the future. The results of MSM diversity research are expected to provide basis for the development of MSM application

青少年体重控制行为量表的编制

目的编制一个适于测量我国青少年体重控制行为的量表。方法采用自编《青少年体重控制行为量表》对兰州市1 020例高中学生进行调查,年龄为（15.4&plusmn;0.6）岁。使用体像忧虑问卷、进食障碍症状自评量表、罗森博格自尊量表和抑郁焦虑压力量表作为校标,运用Mann-WhitneyU检验和Spearman相关分析对数据进行分析。结果健康体重控制行为分量表的内部一致性系数为0.77,一个月之后的重测信度为0.62。不健康体重控制行为分量表内部一致性系数为0.67,隔一个月之后的重测信度为0.56。不健康体重控制行为与体像忧虑（rs=0.26,P&lt;0.01）、进食障碍症状（rs=0.40,P&lt;0.01）、自尊（rs=-0.09,P&lt;0.01）及负性情绪（rs=0.20,P&lt;0.01）等心理指标有着密切的关系。结论青少年体重控制行为量表具有较好的信效度,能够用于测查青少年的体重控制行为。&nbsp;</p