Differential Proteomics Studies on Thermotolerance of Agaricus bisporus

通讯作者: [email protected][中文文摘]利用蛋白质组学和荧光定量PCR方法,研究双孢蘑菇(Agaricus bisporusLange(Imbach))02菌株在常温与高温胁迫下的蛋白质表达差异,发现了6个差异蛋白质点,经NCBI数据库比对分析,表明6个差异蛋白质分别为HSP70家族的HSS1热激蛋白、异柠檬酸裂解酶、细胞色素P450单氧化酶及3个未知蛋白质.这些蛋白质在双孢蘑菇受热胁迫下具有保护自身稳定的功能.[英文文摘]Agaricus bisporus is a kind of cultivated edible mushroom which has high economic value and important ecology significance.Environment temperature is the main restrained factor in its development,and this greatly limits its cultivation season.In this study,2D-eletrophoresis and real-time PCR are employed to discover the proteomic difference in Agaricus bisporus 02 strain after high temperature induction,and 6 differential expressed protein spots were finally obtained.Compared with the NCBI data base,spot 1 is the heat shock protein,spot 2 is isocitrately ase and spot 3 is cytochrome P450 monooxygenase.The other three spots are novel.Presumably, those proteins confer the thermo tolerance on strain 02 under high temperature.国家基础科学人才培养基金(J0630649);国家自然科学基金(30570254)资