Rational Design of Visible-light-active Azobenzene-based Photoswitches for Biochemical Applications

分子光开关在信息技术、生物领域以及分子设备中具有非常重要的地位和应用价值。而偶氮苯分子以其特殊的光化学物理性质，例如顺式-反式光致异构化以及双稳态的结构，在分子光开关中得到了广泛的应用。然而，大多数偶氮苯分子光开关需紫外光照方能进行光致异构化反应，但是，紫外光会伤害生物细胞，且在细胞组织中的穿透能力较差。因此，设计可见光控偶氮苯分子光开关显得极其重要。对平面反式偶氮苯分子的理论研究表明，第一激发态n->pi*跃迁的最大吸收波长在可见光区域，但是由于n->pi*跃迁对称禁阻，吸收峰难以检测。Beharry等人在偶氮苯分子的四个邻位引入甲氧基，实现了可见光控制的顺反异构转变。受他们工作的启发，本论...The molecular photoswitches have promising applications in information technology, molecular devices and biomedical systems, and have attracted increasing interest over the past few decades. Azobenzene ranks first among the available photoswtiches for its fascinating characters of structural trans-cis photoisomerization and bistability. However, most azobenzene-based photoswitches require UV light...学位：理学硕士院系专业：化学化工学院_物理化学(含化学物理)学号：2052011115153

Alternative Formation Mechanism of C_(50)Cl_(10) Fullerene Chloride Based on Density Functional Theory Calculations

过去广泛接受#271C50Cl10是由#271C50空笼直接氯化得到.我们通过研究拓扑结构弄清了C50富勒烯之间的相互关系.利用密度泛函理论(dfT)计算从最稳定C50富勒烯#270C50出发,通过氯化和STOnE-WAlES(SW)转变获得#271C50Cl10.结果表明:氯化后最终产物是热力学最有利的,并且在有氯存在下,SW转变的活化能垒会降低.这些结果可以解释目前的相关实验事实,暗示了#270C50空笼先氯化得到不同#270C50氯化物,再进行两次SW旋转的路径,由于活化能垒更低因而是一条更为可行的路线.#271C50Cl10is widely postulated to be a direct chlorination product of cage#271C50.We suggest an alternative formation mechanism of#271C50Cl10, based on the topological relationship of these C50 fullerenes.Density functional theory(DFT) calculations of the proposed cage transformation pathway in the chlorination of C50 were performed.The proposed pathway is stimulated by chlorination-promoted fullerene cage transformation, with a low activation barrier.DFT calculations of the Stone-Wales(SW) transformation pathways revealed that the thermodynamically favored rearrangement of other C50 chlorofullerene into#271C50Cl10requires a lower activation energy than that of the pristine carbon cage.This suggested that it is a more effective pathway of chlorinating C50to#271C50Cl10.国家自然科学基金(21273177); 国家重点基础研究发展规划项目(973)(2011CB808504)资助~

Development and Application of a Novel Neutralization Test for Echovirus 25

目的:建立一种新型的快速、高通量的埃可病毒25型(ECHO25)中和抗体检测方法,并初步评价其在ECHO25中和抗体筛选和血清流行病学调查中的应用价值。方法:应用免疫荧光方法筛选ECHO25高亲和性抗体并将其作为检测单抗,结合酶联免疫斑点检测技术(ELISPOT)建立ECHO25中和抗体检测方法;使用不同效价的血清评价该方法的准确性;采用所建立的中和方法对ECHO25单克隆抗体、临床血清样品进行检测。结果:建立了快速检测ECHO25中和抗体的Nt-ELISPOT方法,以ECHO25单克隆抗体5B9作为检测抗体;相比经典的中和实验方法 Nt-CPE,该方法可显著缩短检测时间(从5~7 d缩短至1 d以内),检测结果具有较好的一致性;采用所建立的Nt-ELISPOT方法首次筛选获得3株对ECHO25具有较好中和能力的单克隆抗体;临床血清样品检测结果显示厦门地区可能存在ECHO25的流行。结论:该方法可以应用于中和抗体筛选和血清学的临床辅助诊断,为ECHO25的防治研究提供支持。Objective: To establish a rapid and high-throughput neutralization test for echovirus 25(ECHO25),and evaluate its application in neutralizing antibody screening and seroepidemiological surveys. Methods: Immuno-fluorescence assay was applied to screen a high affinity antibody, which was used as the detection antibody forECHO25, and a rapid neutralization test was established based on enzyme- linked immunospot assay(Nt-ELISPOT). The accuracy of this method was evaluated by detecting serum samples with different titer. Monoclonalantibodies against ECHO25 and clinical serum samples were detected via the established neutralization test. Results: A rapid method to detect neutralizing antibody against ECHO25 was established and an anti-ECHO25 anti-body, 5B9, was used as the detection antibody. The detection period could be shortened significantly comparedwith the classical neutralization test(Nt- CPE)(from five to seven days to less than one day), and the Nt-ELISPOT had good consistency with the Nt- CPE. Meanwhile, three neutralizing antibodies for ECHO25 werescreened firstly by this method. The detection results of clinical serum samples showed that infection of ECHO25 might be popular in Xiamen. Conclusion: This method can be used in neutralizing antibody screening and seroepi-demiological surveys, and it may provide support for the control of ECHO25.国家自然科学基金(81371817,81401669

Preparation and Application of Soluble Human Squamous Cell Carcinoma Antigen Expressed by Escherichia coli

旨在建立基于大肠杆菌表达系统的高效可溶性表达人鳞状上皮细胞癌抗原(SCCAg)方法,获得具有较好活性的重组SCCAg抗原并应用于建立抗原检测方法; 。基于pGEX-6P-l载体和大肠杆菌E. coli; ER2566菌株开展重组SCCAg抗原可溶性表达纯化方法研究,评价纯化抗原活性,筛选特异性单克隆抗体,初步建立并评价SCCAg抗原检测方法。结果; 显示,pGEX-6P-l载体和E coli; ER2566菌株可用于建立较高效的可溶性表达和纯化SCCAg抗原的方法,获得了具有较高纯度和活性的重组SCCAg抗原,筛选获得特异性单克隆抗体并; 初步建立了 SCCAg管式化学发光检测方法。建立了有效的基于大肠杆菌表达系统的可溶性表达和纯化SCCAg的方法。The aims of this study are to establish a method for efficient soluble; expression of human squamous cell carcinoma antigen(SCCAg ) based on; Escherichia coli expression system and obtain the recombinant SCCAg; antigen in fine activity, then apply it in the detection method; establishment of antigen. The study on the method of soluble expression; and purification of recombinant SCCAg antigen was conducted based on; pGEX-6P-l vector and E. coli ER2566 strain. The activity of the purified; antigen was evaluated by Abbott Kit and the specific monoclonal antibody; was screened by indirect ELISA. It was proved that PGEX-6P-1 vector and; E. coli strain ER2566 could be used to establish efficient soluble; expression and purification method for recombinant SCCAg antigen.; Moreover, the recombinant SCCAg antigen was proved to be in high purity; and activity. Thus,the SCCAg detection method of chemical luminous tube; was established with the specific monoclonal antibodies. In conclusion,; an effective method for the expression and purification of SCCAg, which; is based on the E. coli expression system, is established.国家高技术研究发展计划(863计划

Effects of Salt and Drought on Winter Wheat in Seedling Stage Under Different Nitrogen Rates

为探究不同浓度盐胁迫和水分胁迫及两者互作对小麦幼苗生理特性的影响,于2017年3月至5月布置盆栽试验,分别设置两个NaCl胁迫(S1,NaCl 1.9g/kg;S2,NaCl 2.9g/kg)和两个水分处理水平(W1,78%田间持水量;W2,47%田间持水量),测定了冬小麦幼苗地上部和地下部干物质量、全氮、叶绿素和可溶性糖含量。结果表明:①在本试验盐胁迫范围内,单一盐胁迫下盐分含量的上升会显著抑制小麦的生长,冬小麦各部分干重、全氮、叶绿素含量明显下降,渗透物质可溶性糖含量会上升;②低盐干旱胁迫互作改善冬小麦幼苗生长状况,叶绿素含量、各部分干物质累积、氮积累量以及可溶性糖含量最大,呈现出对盐旱复合胁迫的适应性;③高盐干旱胁迫互作会加剧对小麦幼苗的生长限制。因此,低盐胁迫下对冬小麦进行适度的干旱刺激可以促进小麦幼苗适应复合胁迫,有利于小麦幼苗生长