Investigation on The Phosphorylation of Peptides and The Interactions between Peptides and Proteins/Deoxyribonucleic Acids

磷酸化修饰是一种重要且常见的蛋白质翻译后修饰方式，蛋白质的可逆磷酸化与去磷酸化过程是真核细胞生命活动中最普遍的调控手段，广泛参与到细胞周期、分化和发育、代谢和神经活动、肌肉收缩和转录调节等生命过程中。 研究蛋白质、多肽的磷酸化方法和磷酸化识别对了解生命过程具有重要科学意义。经典的磷酸化多肽的合成方法，均需要对多肽中的氨基或羧基进行保护。因此，本文的主要工作在于寻找一种水相中非保护的多肽磷酸化方法，为磷酸化多肽的相关研究提供有效的、丰富的样品来源；并且利用电喷雾质谱技术研究磷酸化多肽与蛋白质及活性多肽与核酸的相互作用规律，试图探寻一种通过多肽与生物分子的相互作用，来筛选并利用多级质谱技术同步鉴...Protein phosphorylation is one of the most common and important post translational modifications in cells, and the reversible phosphorylation of proteins regulates nearly every aspect of cell life, such as cell cycle, differentiation and development, metabolism, nerve activity, muscle constriction and transcriptional regulation. Knowing the methods of phosphorylation and its identification is of ...学位：理学硕士院系专业：化学化工学院化学系_化学生物学学号：2052009115133

Electrochemical and In situ FTIR Studies of Maleic Acid Reduction in BF_4

作者自行合成了离子液体[BMIM]BF4,用循环伏安法(CV)、计时电量法(CA)和电化学原位红外反射光谱(in situFTIR),从分子水平考察了离子液体中马来酸在玻碳(GC)电极上的电化学还原过程。结果表明,[BMIM]BF4中马来酸在GC电极上的还原为不可逆过程,测得扩散系数D=9.62×10-8cm2/s;in situFTIRS研究发现,马来酸在离子液体[BMIM]BF4和水溶液中的电还原生成丁二酸的机理不同。在[BMIM]BF4中马来酸还原发生在其中的一个羧基上,即马来酸首先获得一个电子生成阴离子自由基,随后可能获得一个电子生成二价阴离子,或者获得一个电子并在2个H+的作用下生成醛类物质和水。The ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborates(BF4) was synthesized and used as both solvent and electrolyte.Electrochemical reduction of maleic acid in the ionic liquid was studied by cyclic voltammetry(CV),chronoamperometry(CA) and in situ FTIR spectroscopy.The results demonstrated that the reduction of maleic acid on GC electrode is an irreversible process controlled by diffusion.The diffusion coefficient D of maleic acid is determined to be 9.62×10-8 cm2/s.in situ FTIR results illustrated that the reduction of maleic acid in ionic liquids occurs on the carboxyl.The first step is to geaerte radical anions,which are reduced then to aldehyde species and water with two hydrogen ions.国家自然科学基金(批准号:20673091,20833005)~

载银介孔SBA-15吸附剂的制备及其对脂肪酸甲酯的吸附研究

以介孔分子筛SBA-15作为载体,在3-氨基丙基三乙氧基硅烷(APTS)对SBA-15表面修饰的基础上负载银离子制备Ag~+-APTS/SBA-15吸附剂,采用氮气吸附-脱附、X射线衍射(XRD)、傅里叶变换红外光谱(FT-IR)、透射电镜(TEM)、扫描电镜-能谱(SEM-EDS)对吸附剂进行表征,并将吸附剂应用于混合脂肪酸甲酯的分离以考察其吸附性能。氮气吸附-脱附、XRD和TEM分析结果可以看出,制备的吸附剂具有规则有序孔道结构;FT-IR数据显示,介孔SBA-15表面被APTS成功修饰;SEM-EDS结果表明,银离子成功负载到载体SBA-15上;对混合脂肪酸甲酯吸附研究表明,该吸附剂对不饱和脂肪酸甲酯(UFAMEs)吸附效果较好,且随着银离子负载量的增加以及UFAMEs双键数的增多,吸附效果增强;当银离子负载量为25%时,吸附剂对亚麻酸甲酯吸附率高达53.47%。国家重点研发计划项目(2016YFD0600802);;\n厦门大学闽江学者特聘教授科研启动项目(无编号);;\n云南省重大科技专项计划(2018ZG003

Study on removal boron from solargrade silicon with hydrometallurgy

湿法提纯作为冶金法制备太阳能级硅的前处理工序,可以去除大部分金属和硼杂质。研究了以氢氟酸-硫酸混合酸为浸出剂,有机溶剂甲醇作为后处理剂,去除硅粉中硼杂质的方法。采用电感耦合等离子体发射光谱仪(ICP)等对产品进行表征。酸浸过程优化工艺条件:硫酸质量分数为55%,氢氟酸质量分数为7%,酸浸温度为70℃、酸浸时间为4 H、液固质量比为8∶1。酸浸后可使硅粉中的硼杂质质量分数由6.893x10-6降至3.867x10-6,去除率为41.9%。在酸浸基础上采用有机溶剂甲醇作为后处理剂,杂质硼质量分数降至3.84x10-6,去除率为44.29%。从硼酸浸后形成的产物入手探索提高硼去除率的方法,实验验证了该方法的可行性,为研究湿法冶金预处理太阳能级硅提供了新的参考。As a pretreatment unit for preparing solargrade silicon(SG-Si) by metallurgic method,wet purification could remove most metallic impurities and nonmetallic impurities,such as boron.Experiment researched a new method to remove boron from SG-Si with mixed hydrofluoric acid-sulfuric acid as leaching agent and with organic solvent methanol as posttreatment agent.Samples were characterized by ICP and other analysis methods.When SG-Si powder had been leached at optimized conditions as follows:mass fraction of sulfuric acid was 55%,mass fraction of hydrofluoric acid was 7%,reaction temperature was 70 ℃,reaction time was 4 h,and liquid-solid mass ratio 8∶1,it was found that mass fraction of impurity boron in SG-Si was reduced to 3.867×10-6 from 6.893×10-6 and the removal rate was 41.9% after acid leaching;on the basis of the former procedure,mass fraction of impurity boron was reduced to 3.84×10-6 and the removal rate was 44.29% when treated with organic solvent.Experiment proved the feasibility of the method and provided a new reference for researching on the pretreatment of SG-Si by hydrometallurgy

丝组二肽——现代蛋白酶分子进化过程中的原始雏形

系统阐述了我们实验室近十五年来对丝组二肽的生物活性的研究.丝组二肽是目前报道的具有多种切割活性的最小活性肽,它不仅能够切割DNA,而且可以切割蛋白质及羧酸酯.丝组二肽是迷你的磷酸酯酶和蛋白水解酶,是现代蛋白酶分子进化过程中的原始雏形

催化裂解法制备碳纳米管中试反应器优化研究

对催化裂解法(CVD)间歇制备多壁碳纳米管(CNTs)的中试流化床反应器的结构进行优化研究,以期简化结构、改变出料方式、实现半连续化操作。实验结果表明:床层内置加热器开启,CNTs产量下降15%,床层阻力增加67%;预热层高度为零,反应4h,产率最高为9.2g/g;催化剂量为100g,最佳匣钵高度是35cm;将间歇操作调整为半连续操作,生产周期将从24.0h降为8.5h

苯胺嘧啶衍生物X-9通过下调整合素β1表达抑制肝细胞癌迁移侵袭

整合素在许多肿瘤细胞中高表达，并且参与肿瘤细胞的侵袭转移。在肝细胞癌中，整合素β1被报导高表达，并促进肿瘤细胞的侵袭。目前，对于整合素的表达调控癌细胞机制以及干预其表达进而抑制肿瘤细胞转移的研究较少。本研究探讨利用小分子化合物抑制整合素表达来抑制肿瘤细胞迁移和侵袭的可能。首先，对临床肝癌细胞患者癌组织和癌旁组织中的整合素β1的表达进行检测，发现其在癌组织中的表达显著高于癌旁组织（P<0.05）。对TCGA肿瘤数据库的生物信息学分析结果同样显示，整合素β1的高表达与肝癌的分期（P=0.019）和预后（P=0.013）相关。通过筛选发现，苯胺嘧啶衍生物X-9可以抑制肝癌细胞中整合素β1的mRNA和蛋白质的表达（P<0.01）。细胞划痕愈合实验和细胞穿孔结果显示，苯胺嘧啶衍生物X-9能够抑制肝癌细胞的迁移和侵袭（P<0.01）。进一步的研究证实，在肝癌细胞中外源表达整合素β1可以逆转X-9对肝癌细胞迁移和侵袭的抑制；而在敲除整合素β1的细胞中，X-9对细胞的迁移和侵袭的抑制被消除。因此，鉴定出苯胺嘧啶衍生物X-9可以通过下调整合素β1表达，进而抑制肝癌细胞的迁移和侵袭。福建省自然科学基金（No.2016J05087,No.2017J01201）;;福建省卫计委医疗创新项目（No.2015-CXB-15

Molecular phylogenetic analysis of floating Sargassum horneriassociated with green tides in coastal area of Qingdao

2013年6月份,我国黄海连续第7年暴发绿潮灾害,同时出现了漂浮褐藻规模聚集现象,进一步增大了山东半岛绿潮防控工作的难度。为了对漂浮褐藻进行分子鉴定,采用PCR扩增和序列测定的方法,获得了漂浮褐藻的5.8S r DNA-ITS序列。通过与已经报道的其他马尾藻属5.8S r DNA-ITS序列比对及系统发育分析,对绿潮暴发期间山东青岛近海出现的漂浮褐藻样品进行了分子鉴定。结果表明:该漂浮褐藻的5.8S r DNA-ITS基因序列全长1366 bp,其中ITS1序列长度为647 bp,5.8S r DNA序列长度为155 bp,ITS2序列长度为717 bp。序列比对分析显示,2013年绿潮暴发期..

Electrochemical and in situ FTIRS of maleic acid reduction in[BMIM]BF_4 on Pt electrode

采用循环伏安法(CV)、计时电量法(CA)和电化学原位红外反射光谱(in situ FTIRS),从分子水平上研究了离子液体[BMIM]BF_4中马来酸在Pt电极上的电化学还原过程。结果表明,[BMIM]BF_4中马来酸在Pt电极上的还原是受扩散控制的准可逆过程,测得扩散系数为1.0×10~(-7)cm~2·s~(-1);in situ FTIRS研究发现, [BMIM]BF_4中马来酸在Pt电极上的还原发生在其中的一个羧基位上,即马来酸首先获得一个电子生成阴离子自由基,随后再获得一个电子生成二价阴离子。Electrochemical reduction of maleic acid in the ionic liquid was studied by cyclic voltammetry (CV),chronoamperometry(CA)and in situ FTIR spectroscopy.The results demonstrated that the reduction of maleic acid on Pt electrode is a quasireversible process controlled by diffusion.The diffusion coefficient of maleic acid is determined to be 1.0×10~(-7)cm~2·s~(-1).In situ FTIRS results illustrated that the reduction of maleic acid in ionic liquids occurs on the carboxyl.The first step is to generate radical anions, which are reduced to aldehyde species.国家自然科学基金项目(20833005,20873113)。~

Electrochemical Preparation of Fe Core /Pt Shell Nanoparticle Modified Glassy Carbon Electrode and Their Electrocatalytic Activities for Nitrite Reduction

通讯联系人: 陈声培，男，教授; E-mail: shpchen@ xmu． edu． cn; 研究方向:电化学催化、有机电合成、谱学电化学、表面电化学等[中文文摘]通过循环伏安法(CV)在玻碳(GC)电极表面电沉积出分布较为均匀的纳米Fe粒子，制得纳米Fe粒 子修饰的GC(纳米Fe /GC)电极，再经“电荷置换”制得具有Fe核Pt壳结构的纳米粒子修饰的(纳米PtFe / GC) 电极。SEM 结果显示，纳米Fe/GC和纳米PtFe/GC 表面粒子的形貌均呈立方体形，分布较为均匀，粒径在 60 nm 左右。纳米PtFe /GC 电极对亚硝酸盐的还原具有很高的电催化活性。3 种电极的电催化活性顺序依次 为: 纳米Fe /GC ＜ 纳米Pt /GC ＜ 纳米PtFe /GC。相对于纳米Pt /GC 电极，纳米PtFe /GC 电极的起始还原电位 (Ei)正移了0.14V，还原峰电流(ip)增大了3倍。 [英文文摘]Iron nanoparticles were uniformly deposited on glassy carbon electrode by electrochemical cyclic voltammetry，and Fe core/Pt shell nanoparticles ( nano-PtFe) were further obtained through “galvanic replacement”．SEM observation illustrated that cuboid nanoparticles with average sizes，ca． 60 nm，dispersing uniformly on GC electrode surfaces． The electrocatalytical activities for nitrite reduction by nano-Fe /GC， nano-Pt /GC and nano-PtFe /GC electrodes were studied by cyclic voltammetry( CV)． The catalytic activity is in the order of: nano-Fe /GC ＜ nano-Pt /GC ＜ nano-PtFe /GC． The onset potential( Ei ) of nitrite reduction on nano-PtFe /GC electrode is positively shifted 0. 14 V，and the steady reduction current density(ip) is about 3 times of that from nano-Pt /GC electrode．国家自然科学基金(20833005，20873113)、浙江省重中之重学科开放基金(20090503)资助项