Functional groups of benthic macrofauna in the 14th Yong intertidal zone of Nansha,Guangzhou

大型底栖动物根据食性可分为浮游生物食者(planktophagous,Pl)、植食者(phytophagous,Ph)、肉食者(carnivorous,C)、杂食者(omnivorous,O)和碎屑食者(detritivorous,D)五个功能群。本文根据2007–2008年度和2013–2014年度在茳芏(Cyperus malaccensis)、海桑(Sonneratia caseolaris)2种生境调查获得的各4个季度的大型底栖动物数据,分析了广州南沙十四涌潮间带大型底栖动物功能群的生境差异、季节变化和年际变化。2007–2008年度采集到26种大型底栖动物,低于2013–2014年度的36种。无论是2007–2008年度还是2013–2014年度,茳芏、海桑生境的大型底栖动物均以植食者的栖息密度和生物量最高,碎屑食者的栖息密度和生物量最低。生境比较得出,茳芏生境大型底栖动物浮游生物食者(Pl)的丰富度指数(d)、均匀度指数(J)和多样性指数(H')均高于海桑生境。年度比较得出,在茳芏和海桑生境,2013–2014年度浮游生物食者的丰富度指数、均匀度指数和多样性指数均高于2007–2008年度,这是因为2013–2014年度采集到红树蚬(Gelonia coaxans)和彩虹明樱蛤(Morerlla iridescens)等,而2007–2008年度没有采集到。Benthic macrofauna were classified into 5 functional groups,i.e.planktophagous(Pl),phytophagous(Ph),carnivorous(C),omnivorous(O) and detritivorous(D).The biotope difference,seasonal and annual variations of functional group of benthic macrofauna in the intertidal zone of the 14 th Yong of Nansha,Guangzhou were analyzed in this study according to the four seasonal data of benthic macrofauna collected in two biotopes(Cyperus malaccensis and Sonneratia caseolaris) in 2007–2008 and 2013–2014.A total of 26 species were recorded in 2007–2008,which was lower than 36 species in 2013–2014.Both in 2007–2008 and 2013–2014,phytophagous group showed the highest value while detritivorous group shared the lowest one in density and biomass.Habitat comparison showed that the abundance index(d),evenness index(J) and diversity index(H') of planktophagous group in Cyperus malaccensis habitat were all higher than those in Sonneratia caseolaris habitat.Annual comparison showed that the abundance index,evenness index and diversity index of planktophagous group in 2013–2014 were all higher than those in 2007 –2008 both in Cyperus malaccensis and Sonneratia caseolaris habitats because the planktophagous such as Gelonia coaxans and Morerlla iridescens and so on were collected in 2013–2014 but they were not found in 2007–2008.国家自然科学基金(41176089和41376113

戊型肝炎病毒衣壳蛋白特异性人源单克隆抗体的筛选与鉴定

目的:建立从外周血快速筛选戊型肝炎病毒(Hepatitis E virus,HEV)衣壳蛋白特异性人源抗体的方法,从疫苗免疫者外周血中筛选出相应抗体并进行鉴定。方法:采用分选型流式细胞仪获得外周血中HEV衣壳蛋白特异性的记忆B细胞,通过单细胞RT-PCR的方法获得抗体序列,并进行重组表达,最后对获得的人源单克隆抗体进行初步性质鉴定。结果:成功筛选到识别HEV衣壳蛋白的6株人源单克隆抗体,6株抗体均具有抗原结合活性,4株抗体具有中和活性。结论:成功获得HEV衣壳蛋白特异性人源单克隆抗体序列,并进行真核表达,对抗体的性质进行初步鉴定,为后期研究疫苗免疫的人体内的抗体演化打下基础。国家自然科学基金(No.81571996);;福建省自然科学基金(No.2016J05201)资

漳浦县石寨窑发掘简报

漳浦县石寨窑址是闽南一处专门仿烧龙泉青瓷的窑场,从元代开始,一直延续到明初。其产品种类较少,釉色比较丰富。窑业技术颇具特色,出现了介于分室龙窑和横室阶级窑之间的窑炉结构,对研究闽南地区横室阶级窑的起源与发展具有重要意义

Identifying the Conformational Isomers of Single-Molecule Cyclohexane at Room Temperature

构象异构是化学中的基本问题。然而对于环己烷等柔性分子，由于其在室温下极快的互变异构过程，基于系综的表征方法（如核磁等）只能得到所有构象平均贡献的结果。为了应对这一挑战，化学化工学院洪文晶教授与夏海平教授课题组为在室温条件下对柔性分子构象的定量分析与表征这一挑战，课题组成功实现了在室温条件下对环己烷两种椅式构象的电学表征与比例识别。同时，通过纳米电极间隙对分子的限域作用，发现在宏观尺度下极不稳定的扭船式中间体得以在单分子尺度稳定存在，这为不稳定中间体的研究提供了重要表征方法。 这一研究工作是在化学化工学院洪文晶教授、夏海平教授共同指导下完成的，iChEM直博生唐淳与化工系研究生唐永翔为论文共同第一作者。师佳副教授与刘俊扬副研究员为该工作提供了指导，博士后陈志昕、博士研究生陈李珏以及研究生叶艺玲、严哲玮、张珑漪共同参与了该工作。【Abstract】Isomerism reflects the ubiquitous nature that molecules with the same molecular formula show different structures. The interconversion between conformational isomers of flexible molecules is quite fast owing to the low barriers of around 10 kcal mol−1, leading to average signal contributed by all the possible isomers characterized by ensemble methods. On this account, identifying the conformational isomers of flexible molecules at room temperature has a substantial challenge. Here, we develop a single-molecule approach to identify the conformational isomers of cyclohexane at room temperature through the single-molecule electrical characterization. By noise analysis and feature extraction of the conductance of single-molecule junctions, we quantificationally identified two chair isomers of cyclohexane at room temperature, while such identification is only feasible at low temperatures by ensemble characterization. The strategy to apply the single-molecule approach to identify conformational isomers paves the avenue to investigate the isomerization of flexible molecules beyond the ensemble methods.This work was supported by the National Natural Science Foundation of China (nos, 21722305, 21673195, 21703188, and U1705254), the National Key R&D Program of China (2017YFA0204902), China Postdoctoral Science Foundation (no. 2017M622060), and the Fundamental Research Funds for Xiamen University (20720190002).该工作获得了科技部国家重点研发计划、国家自然科学基金等项目的资助，也得到了固体表面物理化学国家重点实验室、能源材料化学协同创新中心的支持

High efficiency multi-jet electrospraying of nano particles with assisted gas

研究了一种带辅助气体的新型纳米颗粒多射流高效雾化喷射技术。开发了带有辅助气流的多喷嘴静电雾化喷头,引导辅助气体均匀分布于各喷嘴四周形成稳定鞘层气流,从而对带电雾化射流产生拉伸与约束作用,而形成的鞘层气流降低了表面电荷密度,克服了带电喷嘴间的电场干扰,实现了多雾化射流的稳定、持续喷射。研究了静电雾化多射流的喷射、沉积行为,分析了辅助气体供气压强对射流喷射临界启动电压和纳米颗粒均匀性的影响规律。实验结果显示:辅助鞘层气流降低了射流喷射临界启动电压和雾化颗粒直径,提升了雾化纳米颗粒的均匀性。辅助气体供气压强从0kPA增加到50kPA时,雾化多射流喷射临界启动电压从4.9kV降低至2.8kV,纳米颗粒平均直径从845.267nM下降至528.06nM。结果表明:鞘层气流的引入为纳米颗粒的多射流、快速喷射提供了一种有效的技术手段,有助于推动静电雾化技术的应用发展。A novel multi-jet electrospray with assisted gas was explored to eject nano particles in a high efficiency.The electrospray spinneret with multi nozzles was designed to guide the assisted gas to form the uniform sheath gas around each nozzle and to provide the excess stretching and restriction forces on the charged electrospray jets.The obtained sheath gas decreases the surface charge density of charged electrospray jets and the interferences among charged jets and makes stable multi electrospray jets for continuously ejection for a long time.The ejection and deposition behaviors of multi electrospray jets were investigated and the effects of sheath gas on the threshold voltage of multi electrospray jet ejection and the uniformity of nano particles were analyzed.The experiments show that the sheath gas is beneficial to the decrease of threshold voltages of multi electrospray jets and the diameters of electrospray nano particles and to the increase of the uniformity of nano particles.When the supply pressure of assisted gas increases from 0kPa to 50 kPa,the threshold voltage of multielectrospray jet ejection decreases from 4.9kV to 2.8kV and the average diameter of nano particles decreases from 845.267 nm to 528.06 nm.It concludes that introducing the assisted gas into electrospray spinneret provides a good way to rapidly fabricate the nano particles through multi-jet electrospray.This work would promote the application research of electrospray technology.国家自然科学基金资助项目(No.51305373); 福建省科技计划重大项目(No.2012H6022); 福建省自然科学基金资助项目(No.2013J05083

Characteristic Analysis of Benthic Meiofauna Communities in Futian Mangrove Area of the Shenzhen Bay

为揭示深圳湾福田红树林区小型底栖动物群落的时空变化,在深圳湾福田红树林区3个取样站进行了夏、秋、冬3个季节的定量取样,共发现7个小型底栖动物类群; ,分别为自由生活海洋线虫、多毛类、寡毛类、双壳类、腹足类、桡足类和昆虫类,还有少许未定类群归为其他类.分析结果显示自由生活海洋线虫是优势类群,它; 与桡足类的丰度比值高,小型底栖动物的平均丰度为(1 572389) ind/(10; ${\rm{c}}{{\rm{m}}^2}$),平均生物量为(814 236)mug/(10; ${\rm{c}}{{\rm{m}}^2}$).单变量双因素方差分析结果表明:不同取样站和季节的小型底栖动物群落的类群数和丰度无显著差异;小型底; 栖动物群落的生物量、类群多样性指数、均匀度指数和丰富度指数呈显著的季节变化,但取样站之间无显著差异.综上,深圳湾福田红树林区小型底栖动物优势类群; 呈现较高的丰度和较低的类群多样性.To probe the spatial and temporal variations of benthic meiofaunal; communities in Futian mangrove area of the Shenzhen Bay,benthic; meiofauna was investigated in three sampling stations in three; seasons.The results showed that seven meiofaunal groups were found,which; were free-living marine Nematoda,Polychaeta,; Oligochaeta,Bivalvia,Gastropoda,benthic Copepoda and Insecta,as well as; some unidentified groups classified as others. Among them,Nematoda was; the dominant group and the nematode/copepod abundance ratio was high.The; average abundance and biomass of meiofauna were (1 572 389) ind/(10; ${\rm{c}}{{\rm{m}}^2}$) and (814236) mug/(10; ${\rm{c}}{{\rm{m}}^2}$),respectively.Univariate two-way ANOVA results; showed that there was no significant difference in group numbers and; meio-funal abundance among seasons and sampling stations. Besides, the; meiofaunal biomass, group diversity index, evenness index and richness; index were significantly different among seasons,while no significant; difference was shown among sampling stations.These results indicate that; the dominant meiofaunal group has higher density and lower value of; group Shannon-Wiener index in Futian mangrove area of the Shenzhen Bay.国家自然科学基金; 海洋公益性行业科研专