“1+1+1”培养模式在高职护生教育实践中的质性研究

目的深入了解高职院校护理实习生接受高职校院合作"1+1+1"培养模式的内心真实体验,分析该模式在人才培养方面的成效。方法通过面对面、深度的半结构式访谈来收集8名护生对该培养模式的切身感受,采用Colaizzi现象学分析法进行资料整理分析。结果提炼出3个主题:(1)该模式促进了其理论知识与临床实践的有机结合;(2)该模式增强了其临床适应能力、人际沟通交流能力和专业认同感;(3)该模式尚存在课程设置欠佳、学习负荷偏重和课余生活单调等不足。结论校院合作"1+1+1"培养模式相比传统模式有明显的优势。但仍需不断优化课程结构、丰富第二课堂活动及减轻学生心理负荷等提升教学成效

Analysis of Mutations of Exon 3 on PS-1 Gene in Alzheimer's Disease

目的探讨阿尔茨海默病(AD)早老素-1(PS-1)基因第3外显子的突变特点。方法采用聚合酶链反应及基因测序技术,对12例血管性痴呆(VD)患者、13例散发性阿尔茨海默病(SAD)患者的PS-1基因第3外显子片段进行扩增与序列分析。结果VD患者PS-1基因第3外显子未发现突变,SAD患者中有1例PS-1基因第3外显子扩增片段发现1处缺失突变。结论在SAD中存在PS-1基因第3外显子突变。Objective To detect the mutations of presenilin-1(PS-1) gene of thirteen Chinese patients with sporadic Alzheimer's disease(SAD).Methods Genomic DNA were isolated from the peripheral blood of the patients with SAD or VD by an initial diagnosis.The exon3 of their PS-1 gene was analysed by using polymerase chain reaction(PCR) and sequencing technique.Results One absence mutation was found on exon3 of PS-1 in one patient,and in which one base(C) was absent.Conclusion It suggests that mutations in exon3 of PS-1 gene exist in patients with SAD.福建省自然科学基金资助项目(C0210008

Spectroscopic Studies on Biosorption of Ag~+

The biosorptive interaction of Ag++ with resting cell of %Lactobacillus sp.% strain A09 has been further studied on a molecular level by means of XPS, EDX, UV-Vis and FTIR techniques. The X-ray photoelectron spectroscopy(XPS) shows that the reductive ratio of the Ag++ to Ag+0 by the A09 biomass reaches to about 54^5% for 3 d. The contain of amino acid residues in dry powder of the biomass such as cysteine, methionine, arginine and lysine, being capable of reducing the Ag++ to Ag+0, are very small in quantity both cysteine and methionine are far less than 0^18%, and both arginine and lysine far less than 0^336%, %via% the analysis with quantitative energy-dispersive X-ray(EDX). The amount of the reducing sugars in the biomass is far larger than 2^71% analyzed by ultraviolet-visible spectrophotometry(UV-Vis). The chemical functional group on cell wass of the biomass such as the carboxylate anion of amino-acid residues seems to be the site for the Ag++ binding and the free aldehyde group of the hemiacetalic hydroxyl from reducing sugars, %i.e.% the hydrolysates of the polysaccharides from the cell wass, plays a protagonist in serving as the electron donor for reducing the Ag++ to Ag+0, characterized by fourier transform infrared(FTIR) spectrophotometry.国家自然科学基金(批准号:29876026)资助

Spectroscopic studies on iosorption of Ag+

The biosorptive interaction of Ag+ with resting cell of Lactobacillus sp. strain A09 has been further studied on a molecular level by means of XPS, EDX, UV-Vis and FTIR techniques. The X-ray photoelectron spectroscopy (XPS) shows that the reductive ratio of the Ag+ to Ag-0 by the A09 biomass reaches to about 54.5% for 3 d. The contain of amino acid residues in dry powder of the biomass such as cysteine, methionine, arginine and lysine, being capable of reducing the Ag+ to Ag-0 are very small in quantity both cysteine and methionine are far less than 0.18%, and both arginine and lysine far less than 0.336%,via the analysis with quantitative energy-dispersive X-ray (EDX). The amount of the reducing sugars in the biomass is far larger than 2.71% analyzed by ultraviolet-visible spectrophotometry (UV-Vis). The chemical functional group on cell wass of the biomass such as the carboxylate anion of amino-acid residues seems to be the site for the Ag+ binding and the free aldehyde group of the hemiacetalic hydroxyl from reducing sugars, i.e. the hydrolysates of the polysaccharides from the cell wass, plays a protagonist in serving as the electron donor for reducing the Ag+ to Ag-0, characterized by fourier transform infrared (FTIR) spectrophotometry