Insulin resistance in the retina: possible implications for certain ocular diseases

Insulin resistance (IR) is becoming a worldwide medical and public health challenge as an increasing prevalence of obesity and metabolic disorders. Accumulated evidence has demonstrated a strong relationship between IR and a higher incidence of several dramatically vision-threatening retinal diseases, including diabetic retinopathy, age-related macular degeneration, and glaucoma. In this review, we provide a schematic overview of the associations between IR and certain ocular diseases and further explore the possible mechanisms. Although the exact causes explaining these associations have not been fully elucidated, underlying mechanisms of oxidative stress, chronic low-grade inflammation, endothelial dysfunction and vasoconstriction, and neurodegenerative impairments may be involved. Given that IR is a modifiable risk factor, it may be important to identify patients at a high IR level with prompt treatment, which may decrease the risk of developing certain ocular diseases. Additionally, improving IR through the activation of insulin signaling pathways could become a potential therapeutic target

The vertical influence of temperature and precipitation on snow cover variability in the Central Tianshan Mountains, Northwest China

Seasonal snow cover in mountainous regions will affect local climate and hydrology. In this study, we assessed the role of altitude in determining the relative importance of temperature and precipitation in snow cover variability in the Central Tianshan Mountains. The results show that: (1) in the study area, temperature has a greater influence on snow cover than precipitation during most of the time period studied and in most altitudes. (2) In the high‐elevation area, there is a threshold altitude of 3900±400 m, below which temperature is negatively while precipitation is positively correlated to snow cover, above which the situation is the opposite. Besides, this threshold altitude decreases from snow accumulated period to snow stable period and then increases from snowmelt period to snow‐free period. (3) Below 2000 m, there is another threshold altitude of 1400±100 m during the snow stable period, below (above) which precipitation (temperature) is the main driver of snow cover

Preparation and imaging of intravascular high-frequency transducer

Intravascular ultrasound (IVUS) imaging is by far the most favorable imaging modality for coronary artery evaluation. IVUS transducer design and fabrication, a key technology for intravascular ultrasound imaging, has a significant impact on the performance of the imaging results. Herein, a 35-MHz side-looking IVUS transducer probe was developed. With a small aperture of 0.40 mm × 0.40 mm, the transducer exhibited a very wide -6 dB bandwidth of 85% and a very low insertion loss of -12 dB. Further, the in vitro IVUS imaging of a porcine coronary artery was performed to clearly display the vessel wall structure while the corresponding color-coded graph was constructed successfully to distinguish necrotic core and fibrous plaque via image processing. The results demonstrated that the imaging performance of the optimized design transducer performs favorably

Fluctuating levels of reprogramming factor expression in cultured human undifferentiated keratinocytes

Although human undifferentiated keratinocytes (HUKs) can be reprogrammed to become induced pluripotent stem cells (iPSCs) with high efficiency and rapid kinetics by transducing reprogramming factors (RFs), the endogenous expression of reprogramming factors in cultured HUKs is not clear at different stages. In this study, keratinocytes were isolated from foreskin of adult subject (18 years old) and cultured on collagen type IV-coated culture dishes in a low-calcium, serum-free medium (Epilife, Invitrogen). In order to clarify the expression patterns of RFs and other stem cell markers in cultured human keratinocytes, total RNA was extracted using Trizol reagent, and polymerase chain reaction (PCR) was performed using established GenBank sequences to design primers. The subsequent PCR analysis was carried out by agarose gel electrophoresis. The expression levels of RFs and other stem cell markers in human HUKs clearly fluctuated during culturing, which supports the hypothesis that HUKs might be reprogrammed into a pluripotent state when the maximum levels of RFs expression are maintained by appropriate culture conditions.Keywords: Human undifferentiated keratinocytes, reprogramming factors, expression fluctuationAfrican Journal of Biotechnology Vol. 12(35), pp. 5389-539

Semantic representation for visual reasoning

In the field of visual reasoning, image features are widely used as the input of neural networks to get answers. However, image features are too redundant to learn accurate characterizations for regular networks. While in human reasoning, abstract description is usually constructed to avoid irrelevant details. Inspired by this, a higher-level representation named semantic representation is introduced in this paper to make visual reasoning more efficient. The idea of the Gram matrix used in the neural style transfer research is transferred here to build a relation matrix which enables the related information between objects to be better represented. The model using semantic representation as input outperforms the same model using image features as input which verifies that more accurate results can be obtained through the introduction of high-level semantic representation in the field of visual reasoning