变性剂对重组牛肠激酶活性的影响

肠激酶(EK,EC 3.4.21.9)是一种在基因工程产品中广泛应用的工具酶.以小分子荧光物质Gly-Asp-Asp-Asp-Asp-Lys-β-萘胺为底物,采用荧光跟踪法研究了变性剂盐酸胍、硫脲、尿素对重组牛肠激酶(rEK)活力的影响及抑制动力学.结果表明:盐酸胍、硫脲、尿素均对该酶的活性有较强的抑制作用,它们对该酶的半抑制浓度(IC50)分别为0.025,0.080和0.750 mol/L.研究的3种变性剂对该酶的抑制均显示可逆抑制机理;抑制类型也均为竞争性类型,测定它们对酶的抑制常数KI分别为0.015,0.060和0.553 mol/L

Series of Ag(I) Coordination Complexes Derived from Aminopyrimidyl Ligands and Dicarboxylates: Syntheses, Crystal Structures, and Properties

通讯作者地址: Huang, RB (通讯作者), Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surface, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surface, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] series of new metal organic frameworks, namely, [Ag(mapym)(suc)(0.5)center dot 0.5H(2)O](n) (1), [Ag-2(mapym)2(glu)center dot 1.5H(2)O] (2), [Ag-2(mapym)(2)(ipa)center dot 2H(2)O](n) (3), [Ag-2(mapym)(2)(tpa)(H2O)](n) (4), [Ag(dmapym)(2)(ox)(0.5)center dot H2O] (5), and [Ag(dmapym)(bbdc)(0.5)center dot 0.5H(2)O](n) (6) (mapym = 2-amino-4-methylpyrimidine, dmapym = 2-amino-4,6-dimethylpyrimidine, H(2)suc = succinic acid, H(2)glu = glutaric acid, H(2)ipa = isophthalic acid, H(2)tpa = terephthalic acid, H(2)ox = oxalic acid, H(2)bbdc = 4,4-bibenzenedicarboxylic acid), have been synthesized and structurally characterized. Complexes 1 and 2 exhibit two-dimensional (2D) interpenetrated and double-sheet structures, respectively. Complex 3 is a three-dimensional (3D) framework constructed through unusual 2D -> 3D parallel interpenetration of a corrugated 2D 4(4)-sql net. Complex 5 is a 0-dimensional (0D) H-shaped molecule, and the self-complementary N-H center dot center dot center dot N hydrogen bonds incorporating R-4(4)(14) hydrogen bond motifs extend these molecules into a 3D supramolecular framework. Complexes 4 and 6 are also 2D sheets constructed with a similar fashion to that of 1 and 2 but with aryl-dicarboxylate as bridge. The results show that the coordination geometry of the Ag(I) ions and the configuration and coordination mode of dicarboxylates as well as the competition between steric and electronic effects of methyl groups on aminopyrimidyl ligands play crucial roles in determining the structure of the complexes. Additionally, results about IR spectra, thermogravimetric curves, and photoluminescence spectra were addressed.National Natural Science Foundation of China 20721001 MSTC 2007CB81530

Chemical constituents of Vitis thunbergii var. taiwaniana

目的研究中国台湾传统中药材小叶山葡萄VITIS THunbErgII VAr.TAIWAnIAnA的化学成分。方法利用大孔树脂、SEPHAdEXlH-20、OdS及正相硅胶柱等色谱手段进行分离,通过多种波谱学数据分析进行单体化合物的结构鉴定。结果从小叶山葡萄60%乙醇提取物中分离得到12个化合物,分别鉴定为白藜芦醇(1)、TrAnS-ε-VInIfErIn(2)、(7r,8r)-THrEO-4,7,9,9′-TETrAHydrOXy-3,3′-dIMETHOXy-8-O-4′-nEOlIgnAn 7-O-β-d-gluCOPyrAnOSIdE(3)、(7S,8r)-urOlIgnOSIdE(4)、SCHIzAndrISIdE(5)、VITISInA(6)、VITISIn b(7)、dAVIdIOl A(8)、3,5,4′-TrIHydrOXySTIlbEnE 4′-O-β-d-gluCOPyrAnOSIdE(9)、蛇葡萄素C(10)、(7r,8S)-dIHydrOdEHydrOdICOnIfEryl AlCOHOl 9-O-β-d-gluCOPyrAnOSIdE(11)、表儿茶素(12)。结论化合物3~5为首次从葡萄属中分离,化合物8、9、11、12为首次从小叶山葡萄中分离。Objective To study the chemical constituents in Vitis thunbergii var.taiwaniana specially grown in Taiwan,China.Methods The compounds were isolated by repeated HP20 macroporous adsorption resin column combined with Sephadex LH-20,ODS,and silica gel column chromatography.Their structures were identified on the basis of extensive spectroscopic data analysis and by comparison of their spectral data reported.Results Twelve compounds were identified as resveratrol(1),trans-ε-viniferin(2),(7R,8R)-threo-4,7,9,9′-tetrahydroxy-3,3′-dimethoxy-8-O-4′-neolignan 7-O-β-D-glucopyranoside(3),(7S,8R)-urolignoside(4),schizandriside(5),vitisin A(6),vitisin B(7),davidiol A(8),3,5,4′-trihydroxystilbene 4′-O-β-D-glucopyranoside(9),ampelopsin C(10),(7R,8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside(11),and epicatechin(12).Conclusion Compounds 3—5 are separated from the plants of Vitis L.for the first time,and compounds 8,9,11,and 12 are separated from V.thunbergii var.taiwaniana for the first time.厦门市重大科技计划项目(3502Z20100006

Synthesis, crystal structure and optical properties of [Ag(UO2)(3)(OAc)(9)][Zn(H2O)(4)(CH3CH2OH)(2)]: A novel compound containing closed-shell 3d(10), 4d(10) and 5d(10) metal ions

[Ag(UO2)(3) (OAc)(9)][Zn(H2O)(4)(CH3CH2OH)(2)] (1, OAc = CH3-COO-) crystallized from an ethanol solution and its structure was determined by IR spectroscopy, elemental analysis, H-1 NMR, C-13 NMR and X-ray crystallography; it is composed of [Zn(H2O)(4)(CH3CH2OH)(2)](2+) cations and [Ag(UO2)(3-)(OAc)(9)](2-) anions in which triuranyl [(UO2)(OAc)(3)](3) clusters are linked by the Ag ion

One-step construction of ZnS/C and CdS/C one-dimensional core-shell nanostructures

ZnS/C and CdS/C coaxial core-shell nanowires, which consist of single-crystal semiconductor nanowires encapsulated by carbon nanotubes ( CNTs), have been synthesized by simple one-step thermal processes. These coaxial core-shell nanowires have uniform outer diameters ( 80-180 nm for ZnS/C, 60-130 nm for CdS/C), and uniform shell thicknesses ( ca. tens of nanometers) along their entire lengths up to tens of microns. The growth of the core-shell nanowires follows a novel metal reaction and self-catalysis vapor-liquid-solid ( VLS) mechanism. The simple method could, in principle, be extended to synthesize other metal sulfide single-crystal nanowire-filled carbon nanotubes, which can find potential applications in various fields of nanotechnology

4H-Cyclo­penta­[def]phenanthren-4-one

In the title compound, C15H8O, the asymmetric unit contains four independent mol­ecules and crystallizes with aromatic π–π stacking inter­actions[centroid–centroid distances = 3.5326 (18) Å]

Synthesis and Structure of O-Ethyl S-Hydrogen Isoquinolin-1-ylcarbonothioimidate

O-Ethyl S-hydrogen isoquinolin-1-ylcarbonothioimidate was synthesized and its structure was characterized by IR, MS, H-1 NMR, elemental analysis and single-crystal X-ray diffraction. The crystal belongs to monoclinic, space group P2(1)/c with a = 10.9471(19), b = 9.3968(17), c = 10.6125(19) angstrom, beta = 100.761(3)degrees, V = 1072.5(3) angstrom(3), Z = 4, D-c = 1.439 g/cm(3), F(000) = 488 and P = 0.279 mm(-1). A total of 7763 reflections were collected, of which 2090 were unique. The structure was refined to the final R = 0.0322 and wR = 0.0882 for 2016 observed reflections with I > 2 sigma(I). The crystal structure shows that there are intramolecular N-(HS)-S-... hydrogen bonds and pi-pi stacking interactions between adjacent molecules

Low dimensional metal-organic frameworks (MOFs) constructed from simple aminopyrimidyl derivatives: From oligomer to single neutral zigzag chain and doubly ionic chains

通讯作者地址: Huang, RB (通讯作者), Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 2. Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R ChinaThree low dimensional silver(1)complexes of the formula [Ag(L-1)][Ag(L-1)(CF3SO3)(2)](1), [Ag-2(L-2)(3)(CF3SO3)(2)] (2), and [Ag(L-2)(CF3CO2)](n) (3), where L-1 = 2-amino-4-methoxy-6-methylpyrimidine and L-2 = 2-amino-4,6-dimethoxypyrimidine, have been synthesized and structurally characterized by single-crystal X-ray diffraction. Complex 1 is a new rarely reported Ag-1 one dimensional (1D)coordination polymer, which consists of independently cat ionic and anionic doubly chains The hydrogen bonds and Ag 0 weak interactions between chains extend 1 into two dimensional (2D) interlayer networks Complex 2 is a simple oligomer and two neighbored oligomers interact to produce a supramolecular dimer through hydrogen bonds, weak Ag N-(amino) interactions and anion-pi interactions Complex 3 displays 1D neutral zigzag chain which is structurally very similar to the 1D cationic one in 1, if neglecting counter anions in the former The adjacent chains in 3 are further interlinked to generate 1D ladder structure via Ag 0 weak interactions and N-H O intermolecular hydrogen bonds. The results reveal that the nature of the Counter anions and organic ligands all has great impact on the structure of the complexes The luminescence properties of the synthesized silver complexes were also investigated in the solid stale at room temperature.National Natural Science Foundation of China 20721001 MSTC 20070815301 Chinese Government Scholarshi

A novel one-dimensional mixed ligands silver(I) coordination polymer containing two different chains

通讯作者地址: Sun, D (通讯作者),Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Shandong, Peoples R China 地址: 1. Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Shandong, Peoples R China 2. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Fujian, Peoples R China 电子邮件地址: [email protected]; [email protected] mixed-ligand coordination polymer (CP) of the formula {[Ag-3(bmimb)(2) (ndca)(1.5)]center dot H2O}(n) (1) (where bmimb = 1,4-bis(2-methylimidazol-1-ylmethyl)benzene and H(2)ndca = naphthalene-1,4-dicarboxylic acid) was synthesized by reaction of Ag2O and bmimb ligand with H(2)ndca under the ammoniacal condition. It has been structurally characterized by element analysis, IR and X-ray single-crystal diffraction. The bmimb acts as bidentate ligand, and the ndca ligand adopts two different coordination modes in 1, which are mu(4)-eta(1):eta(1):eta(1):eta(1) and mu(2)-eta(1):eta(0):eta(1):eta(0), respectively. Coordination of Ag(I) ions with bmimb and ndca ligands produces two different 1D chains, which is attributed to the different coordination modes of the ndca ligand, indicating the ndca ligand plays an important role in the formation of the diverse 1D chains. The complex 1 exhibits photoluminescent emission in the solid state at room temperature. (C) 2012 Elsevier B.V. All rights reserved.National Natural Science Foundation of China 21021061 21071118 MSTC 2007CB815301 Independent Innovation Foundation of Shandong University 2011GN030 Special Fund for Postdoctoral Innovation Program of Shandong Province 20110100

Synthesis, X-ray structures, and photoluminescence of heterometal trinuclear Hg(II)-Pt(I) and tetranuclear Hg(II)-Pd(I) complexes

通讯作者地址: Huang, RB (通讯作者), Xiamen Univ, Dept Chem, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Dept Chem, Xiamen 361005, Peoples R China 2. Xiamen Univ, State Key Lab Phys Chem Solid Surface, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] heterometallic trinuclear complex with monovalent platinum consisting of a [Pt2Hg] cluster bridged by two mu(3)-Cl atoms and another tetranuclear complex with monovalent palladium owning the structural core of open cubane-like [Hg2Pd2Cl2I2] framework were synthesized by self-assembly reactions. (C) 2008 Elsevier B.V. All rights reserved.National Natural Science Foundation of China 20571062 20721001 National Basic Research Program of China 2007CB81530