中国河南两株庚型肝炎病毒(HGV)NS5区部分cDNA的克隆与序列分析

通过逆转录－聚合酶链反应从我国河南省２例重叠感染ＨＣＶ或ＨＢＶ／ＨＤＶ的献血员中，分离到ＨＧＶＮＳ５区的部分ｃＤＮＡ，对其进行序列分析比较，结果表明：河南株ＨＧＶＮＳ５区核苷酸与两株中国ＨＧＶ株的同源性（＞９１．９％）高于国外代表株（８８．５％－９０．６％），但由核苷酸推导的氨基酸的同源性都无明显的地区性区别。ＨＧＶＮＳ５区氨基酸序列较保守（同源性＞９１％），缺乏明显高变区。中国４株ＨＧＶ在７３８４位发生了由Ｃ→Ｔ的变异，从而导致一个共同的保守位点的产生（由Ｆ→Ａ）。重叠感染其他肝炎病毒时，河南株ＨＧＶＮＳ５基因结构并无特异性改

Identification of hepatitis C or/and G virus RNA in one tube by reverse transcription nested polymerase chain reaction

目的庚型肝炎病毒（HgV）与丙型肝炎病毒（HCV）同属黄病毒科，且传播途径相似，重叠感染率高，本研究旨在建立一种同时检测HgV与HCV感染的方法。方法根据HCV与HgV的基因序列分别选取5’-uTr（HCV）与nS3（HgV）的两套引物，在同一管内进行逆转录-巢式聚合酶链反应，并初步应用于153例标本。结果该方法能同时检出HgV与HCV感染，扩增片段大小与设计相符。结论该方法简便特异，适用于临床检测Hepatitis C and G viruses belong to the flaviviridae family.They have similar modes of transmission.The dual infection of HGV and HCV were reported.A simultaneous detection for HCV or/and HGV was established by reverse transcription nested polymerase chain reaction(RT-nested PCR).The primers were derived from the 5'non-coding region of HCV and NS3 region of HGV ,and the length of the PCR product was between 170bp and 300bp.RT-nested PCR can be finished in one test tube and HGV and /or HCV can be detected simultaneously in one RT-nest PCR.153 samples were detected by his method.Our results showed that the established assay is useful for screening and identification of HCV or/and HGV RNA.This method is simple and specific practicable.国家九五课