Cloning and expression of egI gene from Penicillium decumbens L-06

【目的】克隆斜卧青霉l-06的内切葡聚糖酶Ⅰ基因(EgI),并实现其在大肠杆菌内的高效表达。【方法】利用rT-PCr技术克隆了斜卧青霉l-06的内切葡聚糖酶Ⅰ基因(EgI),并将EgI基因克隆到原核表达载体中,构建了重组质粒PET32A-EgI。【结果】转化至大肠埃希菌rOSETTA(dE3),经IPTg诱导重组蛋白表达,SdS-PAgE检测结果表明:重组表达产物的相对分子质量约为80 kd,与预期相符。重组表达的菌悬液,经破碎离心,取其上清液,进行纤维素酶活性染色,获得了活性条带。dnS法测得内切酶活力为2.56 Iu/Ml。【结论】构建了斜卧青霉l-06内切葡聚糖酶Ⅰ的原核表达系统。[Objective] The research focus on cloning endoglucanase I(egI) gene from Peni-cillium decumbens L-06 and expressing in Escherichia coli with high efficiency.[Methods] egI gene was cloned from Penicillium decumbens L-06 by RT-PCR method.Recombinant plasmid pET32a-egI was constructed and was transformed into Escherichia coli rosetta(DE3).Recombinant protein with His-tag was expressed in E.coli rosetta(DE3) after induction with IPTG and then was purified with the Ni-NTA affinity chromatography.[Results] As expected,the relative molecular mass was approximately 80kD after analyzed by SDS-PAGE and Western blotting.Hydrolysis activity of recombinant protein was assayed by cellulase activity staining and DNS method(2.56 IU/mL).[Conclusion] The results achieve the purpose as con-structing prokaryotic expression system and expressing egI gene.国家自然科学基金项目(No.41076106);广东省自然科学基金项目(No.S2011030005257);广东省科技计划项目(No.2009B090300346);广东高校科技创新重点项目(No.CXZD1124

血管介入模拟器在临床医学实习生脑血管病教学中的应用 Application of Vascular Interventional Simulator in Clinical Teaching of Cerebrovascular Disease

摘要： 探讨血管介入模拟器在临床医学实习生脑血管病教学中的应用效果。 方法 选取2020年7月—2021年12月在解放军总医院第八医学中心神经内科实习的61名临床医学本科生为研究对象，其中33名试验组学员采用血管介入模拟器+传统教学模式进行培训，28名对照组学员采用传统教学模式进行培训。实习结束时通过出科理论知识、实践操作考核成绩以及问卷调查评价两种脑血管病带教模式的效果。 结果 试验组学员出科时理论知识[（88.61±4.05）分 vs.（83.43±4.21）分，P＜0.001]、实践操作[（89.73±3.32）分 vs.（85.54±3.17）分，P＜0.001]考核成绩均高于对照组。问卷调查结果显示：试验组学员对神经内科产生兴趣的程度、对脑血管病认识的程度、自学能力、文献阅读水平均优于对照组，差异有统计学意义（均P＜0.05）。 结论 血管介入模拟器应用于脑血管病临床实习带教中提高了实习生对神经内科的兴趣，培养了实习生的学习主动性，提高了脑血管病临床实习的教学效果。 Abstract: Objective To explore the efficacy of vascular interventional simulator in the clinical teaching of cerebrovascular disease for interns. Methods This study included 61 clinical interns in neurology from the Eighth Medical Center of Chinese PLA General Hospital between July 2020 and December 2021, selecting 33 interns as study group and 28 as control group. The study group received training with vascular interventional simulator combined with traditional teaching method, and the control group received traditional teaching method. The efficacy of two teaching methods were evaluated by questionnaire survey, theoretical knowledge examination, clinical operation examination. Results The scores of theoretical knowledge [ (88.61±4.05) vs. (83.43±4.21), P<0.001] and clinical operation examination [ (89.73±3.32) vs. (85.54±3.17), P<0.001] in study group were higher than that in control group. The questionnaires showed that the degree of interesting in neurology and understanding of cerebrovascular disease, ability in initiative learning and the level of literatures reading in study group were all better than that in control group, with statistical differences. Conclusions The application of vascular interventional simulator in clinical teaching of cerebrovascular disease for interns can enhance the interns’ interesting in neurology and learning initiative, significantly improve the efficacy of clinical teaching of cerebrovascular disease

OPGW断股状态的光学监测技术研究

针对OPGW（光纤复合架空地线）易发生断股的现象,提出了采用布里渊光时域反射仪测量其内部光纤应变的断股检测方法。实验中将一段OPGW的两端固定在拉力机上并施加固定的拉力值,逐渐增加OPGW中断开的单丝数,记录布里渊光时域反射仪测量的光纤应变值。实验结果表明,该方法可实现对25%以上的断股区域截面积比的监测,为在线监测OPGW断股状态提供了一种新的思路