A Study on POD and EST Isozyme of Different Strains of Fusarium oxysporum from Different Crops

利用聚丙烯酰胺凝胶电泳测定了来自不同寄主枯萎病原菌尖孢镰刀菌过氧化物酶同工酶(POD)和酯酶同工酶(EST).结果表明,过氧化物酶同工酶共有3条主酶带,其迁移率分别为:Rf=0.08,Rf=0.20和Rf=0.4.但不同寄主的尖孢镰刀菌POD同工酶存在着明显的差别,来源于花生的尖孢镰刀菌只有2条POD同工酶酶带,其迁移率为:Rf=0.20和Rf=0.08;来源于甜瓜的尖孢镰刀菌只有1条POD同工酶酶带,其迁移率为:Rf=0.20;来源于不同寄主的镰刀菌的EST同工酶谱之间也存在着较大的差别.来源于黄瓜、甜瓜和西瓜的镰刀菌的EST酶谱较为相似,都具有Rf=0.018,Rf=0.114和Rf=1.000的3条酶带,其它寄主来源的镰刀菌的EST酶谱间互相则存在着很大的差别.但这些菌株基本上都存在着Rf为0.018和1.000的2条酯酶同工酶酶带.The peroxidase (POD) and esterase (EST) isozyme of different Fusarium oxysporum from different crops were analyzed by polyarcylamide gel eletrophoresis.The results showed that there were 3 main POD isozme bands;the relative migration rates were 0.08,0.24,and 0.4.The strain from the earthnut had only 2 POD isozyme bands and their relative migration rates were 0.20 and 0.08.The strain from melon had only 1 POD isozyme bands and its relative migration rate was 0.20.There were many differences found in esterase isozyme of the different strains of F.oxysporum,in whixh the main isozyme bands were similar in cucumber,melon and watermelon and their relative migration rates were 0.018,0.114,1.000,and that of the F.oxysporum from the other crops was much different.But they all had the main EST isozme bands whose relative migration rates were 0.018 and 1.000.国家863计划项目(2002AA244031 2);; 福建省计委项目(闽计农经[2002]48号)资

Effects of Ultrasonic Treatment on Pathogenicity of Ralstonia solanacearum Strain F.1.3-010703-01

用频率为40kHz,功率为250W的超声波处理野生型番茄强致病力青枯雷尔氏菌F.1.3 010703 01,结果表明:在5、10和20min的处理出现3.24%～5.54%的抑制率,而在15和25min的处理出现-1.04%～-2.82%的抑制率;强致病力菌株的弱化指数在0.4461～0.5376之间,强致病力菌株所占的比例在95.83%～96.14%之间;超声波处理不同时间的菌株回接番茄组培苗,回接发病率皆为100%;处理组与对照之间无极显著性差异(P≥0.01).表明处理菌株保持着强致病力菌株的特性.作为一种机械能量形式,超声波对番茄青枯雷尔氏菌F.1.3 010703 01的生长和致病力都没有影响.作为一种物理致弱因子,超声波对青枯雷尔氏菌无致弱作用.Effects of ultrasonic treatment on the virulent strain,Ralstonia solanacearum F.1.3-010703-01 isolated from tomato were conducted.The result showed that number of the bacteria was slightly reduced to 3.24%～5.54% under 5,10 and 20 min treatments,somewhat increased 1.04%～2.82% under 15 and 25 min treatments.After the ultrasonic treatment,the attenuation index and number ratio of the virulent bacteria were 0.4461～0.5376 and 95.83%～96.14% respectively.The moralities for all in the bioassays infecting the tomato tissue culture plantlet with R.solanacearum treated under ultrasonic were 100%.F text illustrated that there was no significant difference between treatments and control.It was concluded that,as a kind of mechanic energy, ultrasonic had no effect on the pathogenicity of R.solanacearum.All results showed that,as a kind of physical factor,ultrasonic had no attenuation ability to the virulent R.solanacearum.国家863计划项目(2002AA244031 2);; 福建省科技厅重大项目(2000Z031);; 福建省计委项目(闽计农经[2002]48号

Study on the Growing Competition Relationship Between Virulent and Avirulent Strains of Ralstonia solanacearum Isolated from Tomato

在单独和混合培养条件下,研究了番茄青枯雷尔氏菌的强致病力(RV)与无致病力菌株(RA)的生长能力的差异.单独培养时,24h前无致病力菌株的菌体生长量超过强致病力菌株,24h后强致病力菌株菌体生长量超过无致病力菌株,12h时无致病力菌株的生长速率是强致病力菌株的5.6倍,60h时无致病力菌株的菌量仅为强致病力菌株的0.37倍.混合培养时,混合比例RV RA1 and reduced when RVRA=1 or <1;the revised increased ration of the virulent in five mixture treatments were -119.57%～-33.57%.All the amounts of the avirulent strain in five mixture treatments increased,the revised increased ration were 32.03%～346.09%.It was concluded that the avirulent had higher growth ability than the virulent in short time under sole culture condition,and could inhibit the growth of the virulent under mixture culture condition,which could be used as an biocontrol agent for the short-term control efficiency of ABPS to bacterial wilt disease of tomato in the field.国家863计划项目(2002AA244031 2);; 福建省科技厅重大项目(2000Z031);; 福建省计委项目(闽计农经[2002]48号)资

Effect of UV-ray on Virulence of Ralstonia solanacearum for Tomato Bacterial Wilt

紫外线的亚致死剂量、致死剂量对青枯雷尔氏菌作用的实验表明:亚致死剂量照射不同时间对青枯雷尔氏菌强致病力菌株有不同程度地促进作用,而无杀灭作用及任何抑制和致弱现象;致死剂量照射对强致病力菌株和无致病力菌株影响不同,结果差异极显著,无致病力菌株对紫外线的耐受力要高于强致病力菌株.致死剂量紫外线对强致病力菌株处理2min,抑制率即达100%,致弱率为0;无致病力菌株照射2min与4min的处理,抑制率分别为70.3%和96.3%,处理8min以上,抑制率达100%.Lethal dose and sublethal dose of UV-ray acted on Ralstonia solanacearum.The results indicated that different rediation time with sublethal dose could accelerate growth of virulent strain to different degrees,but did not kill it or attenuated its virulence.The virulent strain and avirulent strains both radiated for different time respectively showed remarkable differences of tolerance ability by lethal dose and statistical discrepancy.The tolerance ability to UV-ray radiation for the aviruent strain was better than that for the virulent strain.After 2 min to UV-ray,inhibiting rate of the virulent strain was 100% and attenuation rate was 0,while inhibiting rate of the avirulent strain was 70.3%,96.3% and 100% after radiated by UV-ray for 2 min,4 min and 10 min,respectively.国家863计划项目(2002AA244031 2);; 福建省计委项目(闽计农经[2002]48号)资

Effect of Ventilation on Growth of Fusarium oxysporum Schl.f.sp.niveum

采用摇床转速和装液量来控制不同通气量,对西瓜枯萎病尖孢镰刀菌株X.1.7 030527 02进行培养,研究结果表明:当装液量相同时,尖孢镰刀菌孢子生长量随转速的增加而增加,而当转速150r/min时,尖孢镰刀菌孢子生长量随装液量的变化差异不大,当转速90～120r/min时,尖孢镰刀菌孢子生长量随的装液量增加而减少.装液量越大,平均孢子萌发率越高,100mL/250mL装液量的平均孢子萌发率最高,比50mL/250mL和25mL/250mL分别高1.6%和4.05%.摇床转速对孢子萌发率表现在转速低孢子萌发率高的趋势.菌丝体生长量受通气量(装液量和转速)变化影响较小.Culturing experiments of Fusarium oxysporum Schl.f.sp.niveum X.1.7-030527-02 for the ventilation were carried out under the different liquid volumes and rotate speeds.The results showed the amount of spores produced was positively related to the different liquid volumes under the rotate speeds,in which the more spores were reproduced in the triangle bottle contained with 25 mL/250 mL medium than that with 50 mL/250 mL or 100 mL/250 mL,respectively.The geminating rate of a spore was negatively correlated to the ventilation with the liquid volumes and the rotating speed.But the growth of the fungus was not greatly affected by the ventilation.国家863计划项目(2002AA244031 2);; 福建省计委项目(闽计农经[2002]48号)资

Measurement of integrated luminosity of data collected at 3.773 GeV by BESIII from 2021 to 2024

We present a measurement of the integrated luminosity e+e- of collision data collected by the BESIII detector at the BEPCII collider at a center-of-mass energy of Ecm = 3.773 GeV. The integrated luminosities of the datasets taken from December 2021 to June 2022, from November 2022 to June 2023, and from October 2023 to February 2024 were determined to be 4.995±0.019 fb-1, 8.157±0.031 fb-1, and 4.191±0.016 fb-1, respectively, by analyzing large angle Bhabha scattering events. The uncertainties are dominated by systematic effects, and the statistical uncertainties are negligible. Our results provide essential input for future analyses and precision measurements

Determination of the number of ψ(3686) events taken at BESIII

The number of ψ(3686) events collected by the BESIII detector during the 2021 run period is determined to be (2259.3±11.1)×106 by counting inclusive ψ(3686) hadronic events. The uncertainty is systematic and the statistical uncertainty is negligible. Meanwhile, the numbers of ψ(3686) events collected during the 2009 and 2012 run periods are updated to be (107.7±0.6)×106 and (345.4±2.6)×106, respectively. Both numbers are consistent with the previous measurements within one standard deviation. The total number of ψ(3686) events in the three data samples is (2712.4±14.3)×10^