The study of immunoregulatory activities of hepatic stellate cells in murine heptic tumor microenvironment

原发性肝癌（简称肝癌）是人类最常见的恶性肿瘤之一，因其恶性程度高、预后差被称为“癌中之王”。如今，全世界50%以上的新发和死亡肝癌患者发生在我国，每年我国大约有30万人因患肝癌死亡。随着人们对肿瘤微环境研究的深入，认为肿瘤是在肿瘤细胞与其周围所处的微环境共同作用下形成的，不再只局限于恶变的细胞上。肿瘤微环境是肿瘤在发生发展过程中所处的内环境，包括肿瘤细胞本身、基（间）质细胞、微血管及少量的浸润细胞[1]。其中与肿瘤生成与发展的微环境最直接相关的是基质细胞。基质细胞作为肿瘤微环境中的重要组成部分，在肿瘤的发生发展过程中起着重要调节作用。肝星状细胞(hepaticstellatecell，HSCs...Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide. More than 50% new cases emerging across the globe each year was in China. HCC causes 662,000 deaths worldwide per year, about half of them in China. As the role of the tumor environment in cancer has become better understood, researchers generally consider the tumor cells and the surrounding cells—the microenviron...学位：理学博士院系专业：医学院基础医学系_生理学学号：2452009015373

Orphan receptor TR3 attenuates the p300-induced acetylation of retinoid X receptor a

摘要蛋白翻译后的修饰如磷酸化(phosphorylation)、泛素化(ubiquitination)、类泛素化(sumoylation)和乙酰化(acetylation)在各种生命活动过程中起到了重要的作用。近年来，随着非组蛋白乙酰化的发现，乙酰化修饰越来越成为关注的热点。乙酰化修饰可以调节组蛋白和非组蛋白的各种功能，如转录活性，蛋白与蛋白之间的相互作用，以及蛋白的亚细胞分布等。虽然已经发现许多转录因子包括核受体可以被乙酰化，但是视黄素X受体(retinoidXreceptors,RXRs)能否发生乙酰化及乙酰化如何被调控等问题尚不清楚。在本文研究中，我们发现了RXRa的赖氨酸第145位点可...Abstract Acetylation regulates the functions of histone and nonhistone proteins, including transactivation activity, protein interaction, and subcellular localization. Although many transcription factors, including nuclear receptors, have been shown to be modified by acetylation, whether retinoid X receptors (RXRs) are acetylated and how their acetylation is regulated remains unknown. Here, we pro...学位：理学硕士院系专业：生命科学学院生物医学科学系_细胞生物学学号：20042615

手性固定相Chirex3001对安息香和联萘酚及其类似物的分子识别

利用分子力学方法,建立了苯基甘氨酸型手性固定相Chirex3001的简化模型,并探讨了手性固定相Chirex3001与安息香和联萘酚及其类似物的识别机制.模拟结果表明,固定相主体与手性客体分子识别作用的推动力主要来自于它们之间的π-π堆积、氢键和范德华等作用.主体与(S)-构型的客体1～3结合能力强于(R)-构型的客体,而对于客体4～6,则是与(R)-构型的结合强于(S)-构型,这与高效液相色谱拆分实验结果相符.客体1～3对映体在Chirex3001柱上的分离因子分别为1.02,1.04和1.11,(R)-构型先被洗脱;客体4～6对映体的分离因子分别为1.23,1.26和1.09,(S)-构型先被洗脱

结合电子设计竞赛的“电力电子技术”课程教学改革实践

电子设计竞赛是提高学生动手能力、加强创新设计能力、增进团队合作等能力的有效手段,同时也能促进高校相关课程的改革。\"电力电子技术\"课程涉及电子技术、控制技术和电力技术,主要内容包括电力电子器件、整流电路、逆变电路、直流-直流变换、交流-交流变频、PWM控制技术及软开关等,课程实践性和工程性都很强。根据\"电力电子技术\"课程特点及存在的问题,提出相应的课程教学改革思路并加以实践,包括将\"电力电子技术\"课程与电子设计竞赛结合,把电赛题目带进课堂,通过案例式教学讲授知识点,积极动员学生参与电子设计竞赛,积累好的作品反馈于课程教学等。结果表明:结合电子设计竞赛的\"电力电子技术\"课程选课学生数有明显提升,参与电子设计竞赛的学生人数和获奖比例有一定提高

Biological characteristics of mesenchymal stem cells from bone marrow versus adipose tissue in rats

背景:除骨髓外,人们已从胎盘组织、脐带血肌肉组织、脂肪组织中分离到了间充质干细胞。目的:比较大鼠脂肪和骨髓间充质干细胞生物学特性和免疫调节功能的差异。方法:脂肪间充质干细胞和骨髓间充质干细胞分别来自bn大鼠的脂肪和骨髓。体外分离、纯化脂肪和骨髓来源间充质干细胞,进行细胞形态、表面标志、生长动力学、分化潜能鉴定;混合淋巴细胞反应比较两种细胞的免疫调节特性。结果与结论:脂肪间充质干细胞与骨髓间充质干细胞光镜和透射电镜下形态相似,第3代的脂肪间充质干细胞和骨髓间充质干细胞均高表达Cd29,Cd90,低表达Cd34,Cd45,Cd11b;第3,4,5代脂肪间充质干细胞增殖速度明显快于骨髓间充质干细胞;两者都具有低免疫原性,可以抑制异基因抗原引起的T淋巴细胞增殖,且这种抑制作用与细胞数目成正相关,等量脂肪间充质干细胞和骨髓间充质干细胞抑制作用差异无显著性意义。结果证实脂肪间充质干细胞具有和骨髓间充质干细胞同样的低免疫原性和免疫调节功能。BACKGROUND:Except bone marrow,people have collected mesenchymal stem cells (MSCs) from placenta tissue,cord blood muscle tissue and adipose tissue.OBJECTIVE:To compare the difference in biological characteristics and immunoloregulation of rat adipose-derived MSCs (ADSCs) and bone marrow MSCs (BMSCs).METHODS:ADSCs and BMSCs were obtained respectively from adipose and bone marrow of BN rats.ADSCs and BMSCs were isolated and purified in vitro to determine cell morphology,surface marker,growth kinetics and differentiation potential.Immunoloregulation of ADSCs and BMSCs was compared in mixed lymphocyte response RESULTS AND CONCLUSION:Morphology was similar between ADSCs and BMSCs under an optical microscope and transmission electron microscope.ADSCs and BMSCs at passage 3 highly expressed CD29 and CD90,weakly expressed CD34,CD45 and CD11b.The proliferation speed of ADSCs at passages 3,4 and 5 was obviously faster than BMSCs.Both ADSCs and BMSCs possessed low immunogenicity,could suppress allogenic antigen-induced T lymphocyte proliferation.Moreover,this inhibitory effect was positively associated with cell number.There were no significant differences in inhibitory effects of an equal volume of ADSCs and BMSCs.Results verified that ADSCs had the same low immunogenicity and immunoloregulation as BMSCs.卫生部科学研究基金——福建省卫生教育联合攻关计划资助(WKJ2005-2-020);厦门市科技计划社会发展项目(3502Z20064020)资助课题名称:外周血干细胞移植诱导肝移植术后免疫耐受的实验研究---

人脂肪间充质干细胞的分离培养及其鉴定

背景:人脂肪间充质干细胞在不同的条件下被成功诱导分化为成骨细胞、软骨细胞、骨骼肌细胞、肝脏细胞等中胚层细胞,同时也可以向其他胚层分化,证明脂肪间充质干细胞是多分化潜能干细胞。目的:探讨人脂肪间充质干细胞的分离、鉴定方法。方法:整形外科吸脂术获得腹部皮下脂肪,0.075%Ⅰ型胶原酶消化,贴壁培养获得人脂肪间充质干细胞,绘制细胞增殖曲线,进行流式细胞、细胞周期、免疫荧光等检测,并验证其是否具有多功能分化潜能。结果与结论:获得形态较均一的长梭形的人脂肪间充质干细胞,细胞增殖良好,有明显的指数生长期,体外能长期培养存活,并保持不分化状态;流式检测细胞高表达CD105、CD90,低表达CD34、CD45;多数细胞周期检测G0/G1期占80%以上。能向成脂、成骨诱导分化。结果表明,实验分离获得的细胞是具有脂肪间充质干细胞特性的细胞,为深入研究人脂肪间充质干细胞打下基础

TLR4的表达与急性胆道梗阻时内毒素致小鼠肝脏损伤的关系

目的探讨急性胆道梗阻时内毒素损伤小鼠肝脏的机制及其与TLR4表达的关系。方法雄性C57BL/10J(WT)小鼠42只,随机分为生理盐水组(NS组,n=21)、内毒素处理组1(LPS1组,n=21),C57BL/10ScnJ(TLR4-/-)小鼠21只,为内毒素处理组2(LPS2组)。3组均行胆总管结扎术,LPS1、LPS2组小鼠于胆总管内注射LPS(8ng/μL,10ng/g体重),NS组注射同等剂量的生理盐水,术后6、12、24h采集标本,RT-PCR检测肝脏组织TLR4mRNA的表达情况,全自动生化分析仪检测血清ALT、TBIL、DBIL水平,ELISA法检测血清TNF-α、IL-6的水平。病理观察肝脏损伤情况,免疫组织化学染色观察肝脏NF-κB的表达。结果 LPS1组与NS组比较肝脏组织TLR4mRNA在6h时表达已有升高,于24h达高峰,ALT、TBIL各时点均明显升高(P<0.01),TNF-α、IL-6表达亦增高(P<0.01),LPS1病理损伤程度较NS组重,免疫组化显示术后24小时NF-κB在LPS1组可见肝细胞明显的核表达。LPS2组与LPS1组比较各血清学指标均明显下降,病理损伤减轻,24h时肝细胞NF-κB核表达较少。结论在急性胆道梗阻时LPS可以加重肝脏组织损伤和机体炎症反应,可能与TLR4的表达增高及NF-κB的表达有关。阻断LPS-TLR4信号通路可以减轻LPS引起的机体损伤

Molecular recognition of benzoin and bi-beta-naphthol analogues by Chirex 3001-based chiral stationary phase

The simplified model of (R)-phenylglycine based chiral stationary phase (Chirex 3001) was built to investigate the process of chiral recognition of benzoin and bi-beta-naphthol and their analogues by molecular mechanics methods. The results showed that the driving force of chiral recognition came from the pi-pi stacking, hydrogen bond and van der Waals interactions between the host and the guest molecule. The host preferred to bind with S-enantiomers of benzoin and its analogues 1 similar to 3, and preferred to bind with R-enantiomers of bi-beta-naphthol and its analogues 4 similar to 6. This was consistent with the HPLC experimental results. The separation factors were 1.02, 1.04 and 1.11 respectively for compounds 1 similar to 3 when n-hexane/1,2-dichloroethane/ethanol (V : V : V=83.5 : 15 : 1.5) was used as mobile phase, and the R-enantiomers were eluted first. The separation factors were 1.23, 1.26 and 1.11 respectively when n-hexane/chloroform/ethanol (V : V : V=78.5 : 20 : 1.5) was used as mobile phase, and the S-enantiomers were eluted first

Attitude Detection of Mobile Robot Based on Complementary Filter Algorithm

移动机器人的姿态检测能够反映出机体相对于路面的实时姿态,有利于机器人对行进过程中的当前姿态做出相应的控制。针对移动机器人姿态检测的要求,采用MEMS陀螺仪和加速度计对移动机器人在移动过程中相对于路面的姿态倾角进行测量并通过互补滤波算法对其测量的角度进行解算。互补滤波算法将加速度计静态特性和陀螺仪动态特性有效融合到一起,并对互补滤波算法进行了微分处理,提高了系统的动态特性,计算量小,从而实现对机器人行进过程的路面起伏状况进行实时测量