Quantitative analysis of β1,6GlcNAc-branched N-glycans on β4 integrin in cutaneous squamous cell carcinoma

α6β4 integrin plays pivotal roles in cancer progression in several types of cancers. Our previous study using N-glycan-manipulated cell lines demonstrated that defects in N-glycans or decreased β1,6GlcNAc-branched N-glycans on β4 integrin suppress β4 integrin-mediated cancer cell adhesion, migration, invasion, and tumorigenesis. Furthermore, immunohistochemical analysis has shown that colocalization of β1,6GlcNAc-branched N-glycans with β4 integrin was observed in cutaneous squamous cell carcinoma (SCC) tissue. However, until now there has been no direct evidence that β1,6GlcNAc-branched N-glycans are upregulated on β4 integrin in cutaneous SCC. In the present study, we performed an ELISA analysis of β1,6GlcNAc-branched N-glycans on β4 integrins as well as β4 integrins in cell lysates from human normal skin and cutaneous SCC tissues. The SCC samples showed a 4.9- to 7.4-fold increase in the ratio of β1,6GlcNAc-branched N-glycans to β4 integrin compared with normal skin samples. These findings suggest that the addition of β1,6GlcNAc-branched N-glycans onto β4 integrin was markedly elevated in cutaneous SCC tissue compared to normal skin tissue. The value of β1,6GlcNAc-branched N-glycans on β4 integrin may be useful as a diagnostic marker associated with cutaneous SCC tumor progression

D3h -Symmetric Porphyrin-Based Rigid Macrocyclic Ligands for Multicofacial Multinuclear Complexes in a One-Nanometer-Sized Cavity.

The one-step synthesis of D3h -symmetric cyclic porphyrin trimers 1 composed of three 2,2\u27-[4,4\u27-bis(methoxycarbonyl)]bipyridyl moieties and three porphyrinatozinc moieties was achieved from a nickel-mediated reductive coupling of meso-5,15-bis(6-chloro-4-methoxycarbonylpyrid-2-yl)porphyrinatozinc. Although cyclic trimers 1 were obtained as a mixture that included other cyclic and acyclic porphyrin oligomers, an extremely specific separation was observed only for cyclic trimers 1 when using columns of silica gel modified with pyrenylethyl, cyanopropyl, and other groups. Structural analysis of cyclic trimers 1 was carried out by means of NMR spectroscopy and X-ray crystallography. Treatment of an η(3) -allylpalladium complex with a cyclic trimer gave a tris(palladium) complex containing three η(3) -allylpalladium groups inside the space, which indicated that the bipyridyl moieties inside the ring could work as bidentate metalloligands.The one-step synthesis of D3h -symmetric cyclic porphyrin trimers 1 composed of three 2,2\u27-[4,4\u27-bis(methoxycarbonyl)]bipyridyl moieties and three porphyrinatozinc moieties was achieved from a nickel-mediated reductive coupling of meso-5,15-bis(6-chloro-4-methoxycarbonylpyrid-2-yl)porphyrinatozinc. Although cyclic trimers 1 were obtained as a mixture that included other cyclic and acyclic porphyrin oligomers, an extremely specific separation was observed only for cyclic trimers 1 when using columns of silica gel modified with pyrenylethyl, cyanopropyl, and other groups. Structural analysis of cyclic trimers 1 was carried out by means of NMR spectroscopy and X-ray crystallography. Treatment of an η(3) -allylpalladium complex with a cyclic trimer gave a tris(palladium) complex containing three η(3) -allylpalladium groups inside the space, which indicated that the bipyridyl moieties inside the ring could work as bidentate metalloligands

退院支援に係る診療報酬の確実な取得に向けた取り組み

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新颖的二氧化钛-石墨烯纳米复合材料及其制法和应用

本发明涉及用于制备石墨烯的方法、利用芘、芘衍生物、萘、萘衍生物中的一种或二种以上作为改性剂来进行石墨烯表面改性的方法，以及利用经表面改性后的石墨烯来与含钛化合物反应并制得TiO2-石墨烯纳米复合材料的方法及其产物。本发明用于制备石墨烯的方法包含(a1)在惰性溶剂中用还原剂对分散的氧化石墨进行还原，从而形成含石墨烯的反应混合物，其中所述还原剂包括硼氢化钠、维生素C或其组合；和(a2)从所述反应混合物中分离出所述的石墨烯。本发明制得的石墨烯为絮凝状并且TiO2-石墨烯纳米复合材料具有高效光催化性能

可自支撑超薄透明导电碳纳米管薄膜及其制备方法与应用

本发明公开了一种可自支撑超薄透明导电碳纳米管薄膜及其制备方法与应用。本发明通过将碳纳米管分散液转移至选定溶液表面，再移除该选定溶液，获得碳纳米管薄膜；以及，将所述碳纳米管薄膜转移至油墨界面上进行自组装而形成碳纳米管杂化薄膜。本发明的可自支撑超薄透明导电碳纳米管薄膜综合性能良好，例如其厚度约20nm～100nm，表面粗糙度小于1.5nm，透光度大于86％，表面电阻小于1500Ω-m2，同时其制备工艺流程简单、操作方便、对环境因素要求低，且不会对周围环境不造成不利影响，能够满足工业化大面积、大批量生产的需求

一种图案化3D石墨烯导电薄膜及其绿色制备方法和应用

本发明涉及一种图案化3D石墨烯导电薄膜及其绿色制备方法和应用。所述方法包括步骤：1)提供图案化印章、氧化石墨烯分散液和基底；2)将所述氧化石墨烯分散液涂覆在所述图案化印章上，得到涂覆氧化石墨烯薄膜的图案化印章；3)所述涂覆氧化石墨烯薄膜的图案化印章翻转在基底上，在图案化印章上表面进行滚压处理得到氧化石墨烯薄膜；4)还原所述氧化石墨烯薄膜，得到图案化3D石墨烯导电薄膜。所述制法具有操作简单、成膜时间短、成本低廉、环境友好、利于实现大规模生产的特点。所述图案化3D石墨烯导电薄膜结构稳定、柔性强度高、导电率高且图案清晰度高