核电站主蒸汽系统冗余压力传感器异常检测

为保障核电站运行安全,电站设置冗余传感器对重要系统的关键参数进行测量,传感器的健康状况将直接影响测量结果.在被测冗余传感器个数较少,为3个左右时,目前核电站常用的传感器校准方法很难区分故障传感器.将独立成分分析和序贯概率比检验(independent component analysis-sequential probability ratio test,ICA-SPRT)相结合的方法用于冗余传感器故障检测,使用核电站主蒸汽系统冗余压力传感器数据进行验证.与简单平均法的结果进行对比可知,该方法在冗余度不高的情况下明显优于简单平均法,能及时准确地检测到传感器信号的漂移

东洞庭湖鱼类稳定同位素的季节变化

水位波动是影响湖泊、河流等水体生态系统功能的主要因子,对湖泊沿岸带及水体与陆源间的物质和能量交换起着重要作用[1],因而也是湖泊生态恢复和生态管理中备受关注的重要问题之一。水位的变化可引起水体的理化性质及生活其中的生物种群结构发生变化,从而导致食物网结构在高水位和低水位时期表现不同的差异。洞庭湖(N 28°44′—29°35′,E 111°53′—113°05′)属于典型的季节性吞吐型湖泊,水位季节变化和年际变化显著,丰、

东洞庭湖鱼类稳定同位素的季节变化

水位波动是影响湖泊、河流等水体生态系统功能的主要因子,对湖泊沿岸带及水体与陆源间的物质和能量交换起着重要作用,因而也是湖泊生态恢复和生态管理中备受关注的重要问题之一。水位的变化可引起水体的理化性质及生活其中的生物种群结构发生变化,从而导致食物网结构在高水位和低水位时期表现不同的差异。洞庭湖（N 28°44′—29°35′,E 111°53′—113°05′）属于典型的季节性吞吐型湖泊,水位季节变化和年际变化显著,丰

东洞庭湖鱼类稳定同位素的季节变化

水位波动是影响湖泊、河流等水体生态系统功能的主要因子,对湖泊沿岸带及水体与陆源间的物质和能量交换起着重要作用,因而也是湖泊生态恢复和生态管理中备受关注的重要问题之一。水位的变化可引起水体的理化性质及生活其中的生物种群结构发生变化,从而导致食物网结构在高水位和低水位时期表现不同的差异。洞庭湖（N 28°44′—29°35′,E 111°53′—113°05′）属于典型的季节性吞吐型湖泊,水位季节变化和年际变化显著,丰

Studies on trophic niches of Macrobrachium nipponensis and Exopalaemon modestus

This study was undertaken to assess the trophic niches and niche widths between two freshwater shrimps, Macrobrachium nipponensis and Exopalaemon modestus, from the Yangtze River-connected lakes-Dongting lake and Poyang lake-using stable carbon and nitrogen isotope analysis. The overall aim was to provide the information valuable for evaluation of trophic relationships and for protection of natural resources. Results showed significant differences in δ 13 C values between M. nipponensis and E. modestus, but no difference in δ 15 N values between two species. Meanwhile, differences in isotopic variations of shrimps were found between lakes which had differences in nutrient level and in hydrology. The range of δ 13 C signatures in M. nipponensis was greater than those of E. modestus and mainly distributed from -25.0‰ to -23.0‰, indicating that M. nipponensis fed on more food sources but preferred a certain one. There was a large overlap of trophic niches between the two species. This result indicated that food consumed were nearly the same for both species, implying similar trophic positions and strong inter-composition. In the Poyang Lake, M. nipponensis had a trophic niche and niche width larger than E. modestus. However, there were opposite observations in the Dongting Lake due to variations of sampling habitats and the degree of human disturbances

露地越冬葡萄枝条水分含量与抗旱寒性关系研究

在露地越冬的条件下,以抗寒性较强的几个种间杂交葡萄品种枝条为试材,通过研究不同时期枝条总含水量、自由水、束缚水和枝条萌芽率的变化,分析葡萄枝条水分含量与萌芽率的关系,确定品种抗旱寒性能,并根据水分与萌芽率的隶属分析确定水分含量临界值。结果表明:葡萄枝条抗旱寒性与水分含量各指标均具有相关性,其中与枝条总含水量、束缚水含量显著正相关,与失水量显著负相关,相关系数均达0.896以上。露地越冬期间,枝条总含水量及束缚水含量越高,失水量越小,葡萄失水死亡的几率就越小,枝条萌芽率越高,抗旱寒性越好。‘北红’‘新北醇’‘北玫’‘北玺’‘北馨’等5个品种在宁夏产区2018年冬季至2019年春季的气候条件下可以露地安全越冬,具有较强的抗旱寒性能,而‘威代尔’在露地不能安全越冬,应采取防护措施。研究还发现,冬季枝条含水量和抗旱寒性能还与树龄有关

河流型硅藻水华研究进展

通过对硅藻水华发生机制相关文献的研究分析,对硅藻水华发生机制做了一般性探讨,并重点关注了河流型水华的发生机制的独特点。河流水华种类主要为中心硅藻纲,静水生态系统水华种类除中心硅藻纲还包括部分羽纹硅藻纲种类。河流水华硅藻种类对营养盐浓度、温度和光照等环境因子具有一定的适应范围。与静水生态系统相比,河流硅藻水华受气象和水文等物理指标的影响更为明显。河流硅藻水华中常具有时滞现象,其中由气象和水文因素引起的时滞现象最为明显。目前河流硅藻水华的防治仍然以上游水库下泄稀释为主,但是这种方法会造成一定的水资源浪费。河流水华硅藻的生理属性、硅藻水华对生态系统的影响、节水与抑制水华的统筹以及其他控制水华的方法都是未来河流硅藻水华研究的重点

葡萄多种群种间杂种后代抗病性特征研究

本研究以含山葡萄血统的欧山杂种与含有河岸葡萄、美洲葡萄、欧亚种葡萄血统的杂种后代进行杂交获得的多种群杂交群体后代16个典型株系为材料,以‘北玫’‘北红’‘威代尔’‘贵人香’为对照;通过田间自然鉴定法和室内接种鉴定法,鉴定了露地越冬葡萄多种群种间杂种后代对霜霉病和白粉病的抗性。结果表明:杂种优系表现出明显的抗病优势。田间调查发现,有15个优系对霜霉病表现高抗和抗性,12个优系对白粉病表现免疫和高抗。在室内条件下接种霜霉病后,有11个优系为高抗或抗性级别,明显优于对照品种。因此认为,利用含美洲血统的葡萄基因可选育抗病性能强的新优品种,本试验中的优系‘5-1-247’和‘5-1-444’表现抗病性极好,明显优于其他优系

ECHS1 Involved in Antagonising Apoptosis in HepG2 Cells via the Mitochondrial Pathway

目的:观察烯脂酰辅酶A水合酶短链1(EnOyl-COA HydrATASE SHOrT CHAIn 1,ECHS1)对HEPg2细胞凋亡的影响。方法:构建ECHS1基因的干扰质粒,转染HEPg2细胞后通过嘌呤霉素筛选稳定干扰ECHS1的细胞株(HEPg2-SIECHS1),利用WESTErn blOT验证其干扰效率;利用流式细胞仪及TunEl方法检测ECHS1干扰后HEPg2细胞凋亡情况,再采用WESTErn blOT检测凋亡相关蛋白表达水平。结果:成功构建了ECHS1的干扰质粒;WESTErn blOT验证了ECHS1干扰后HEPg2细胞株中ECHS1表达低于空白对照组(未转染HEPg2细胞)及阴性对照组(空载体Pu6转染HEPg2细胞)(P<0.05);流式细胞仪术显示HEPg2-SIECHS1组细胞株凋亡率(14.98±1.07)%,阴性对照组(10.55±0.19)%,两者差异有统计学意义(P<0.05);TunEl实验显示HEPg2-SIECHS1组细胞株凋亡率(6.13±0.12)%,阴性对照组(2.89±0.21)%,两者差异有统计学意义(P<0.05);与阴性对照组比较,ECHS1干扰后P53、促凋亡蛋白bAX及bId均表达增加。结论:ECHS1通过线粒体途径拮抗HEPg2细胞凋亡。Objective: To investigate the impact of enoyl-CoA hydratase short chain 1(ECHS1) on apoptosis of HepG2 cells.Methods: The ECHS1 interference plasmids were constructed and transfected into HepG2 cells.The stable interference cell lines were screened via puromycin.Interference efficacy was detected by Western blot.Flow cytometry and TUNEL assays studied the apoptosis of HepG2 cells.Apoptosis-related proteins were detected by Western blot.Results: The HepG2 cells with stable ECHS1 gene interference were successfully established.The expression level of ECHS1 protein in HepG2 cells after transfection with ECHS1 siRNA was significantly lower than that in the blank control cells(HepG2 cells without transfection) and the negative control cells(HepG2 cells transfected with pU6 vector)(P< 0.05).Apoptosis ratio of the ECHS1 siRNA group was significant higher than that of negative control group by both Flow cytometry and TUNEL assays(P<0.05).Expressions of p53,Bax and Bid in ECHS1 siRNA group were higher than those in negative control group.Conclusion: ECHS1 may antagonise the apoptosis of HepG2 cells via the mitochondrial pathway.南京军区医学科技创新课题(10MA070); 厦门市科技计划医疗卫生创新项目(3502Z20124029)资助项

Spatial Distribution Characteristics of Metals in New-born Coastal Wetlands in the Yellow River Delta

采用样带布点法,研究了黄河三角洲新生滨海湿地从黄河岸边至盐滩不同植物带上12种金属元素（Na、Mg、Ca、Al、Cr、Pb、Sr、Co、Ga、V、Ni和Ti）空间分布特征。结果表明,由黄河岸边至盐滩方向,除Ti以外,土壤中其他11种金属元素含量均呈逐渐升高的趋势;0～30cm表层土壤中各金属元素含量均高于内陆沼泽;将土壤背景值作为标准,黄河三角洲土壤重金属Cr、Pb和Ni处于轻微污染状态;各金属元素在剖面上的变化差异较大,总体上Na和Sr含量随着剖面深度的增加而逐渐升高,Ca和Mg则表现出相反的规律,Pb、Co、Ga、V、Ni和Ti在剖面上出现了近S形的变化规律。相关分析表明,研究区域土壤中总有机碳含量、总氮含量、硝态氮含量与Ca、Mg、Al、V、Co、Ga、Sr、Cr、Ni、Pb含量都呈极显著正相关,说明黄河三角洲新生湿地土壤总有机碳含量、总氮含量和硝态氮含量是影响土壤吸附金属元素的主导因素