The Current Situation and Developmental Strategy of the Marine Technology Experimental Teaching

该文针对海洋技术实验教学现状,提出实验教学改革和实验室建设的几点建议,以适应培养面向21世纪海洋技术合格人才的需要。According to the current situation of the marine technology experimental teaching, some suggestions about the experiment teaching revolution and the laboratory developments were given to meet the exigency of training the qualified technicians in marine technology in the new century

Proteomics analysis of Arabidopsis calcium deficient mutant and wild type plants during osmotic stress

干旱和盐渍是最常见且对植物危害严重的两个非生物胁迫因子，其作用的实质都是引起植物细胞的渗透胁迫，而胞内Ca2+浓度的瞬时升高是植物响应渗透胁迫中检测到的最早事件之一。研究证明，胞内Ca2+浓度的升高引发的钙信号主要通过三条途径（Ca2+/CaM、Ca2+/CDPKs、Ca2+/CBLs）向下游放大、传导，进而在细胞内引起一系列的生化反应以抵抗或适应各种逆境胁迫。但有关植物如何在分子水平感受外界信号，并将这些信号转换为胞内信号的环节上仍知之甚少。 本研究以PeiLab提供的拟南芥突变体cio4（该突变体为渗透胁迫下胞内Ca2+增加缺陷型突变体）与其野生型Col/AQ为研究对象，利用双向电泳技术...Drought and salt are the most common abiotic stressors that often cause great harm to plants by osmotic stress. The instantaneous increasing of concentration of cytosolic Ca2+ is one of the primary events that can be detected in this processses. It have been proved that the Ca2+ signal is amplified and transmitted by three main signal pathways (Ca2+/CaM、Ca2+/CDPKs、Ca2+/CBLs), and induces conseque...学位：理学硕士院系专业：生命科学学院生物学系_发育生物学学号：2172009115203

A Study on Unmarried Cohabitation among College Students

随着大学生婚育权的解禁，大学生非婚同居将成为高校面临的另一个棘手的管理问题。法律在非婚同居问题上的缺位，不仅无法阻止大学生非婚同居的发展，反而影响了大学生非婚同居问题的解决，增加了高校和社会的负担。在新的《普通高等学校学生管理规定》实施近三年后，现实中大学生的婚育状况如何呢？大学生的非婚同居和婚前性行为的情况如何呢？如何进行规制呢？笔者利用自身在高校从事学生工作的优势，通过问卷调查、访谈调查和查找资料等方式，对大学生的非婚同居现象进行了实证调查研究。 本文除了引言和结论外，分为三章： 第一章大学生非婚同居概述。本章介绍了非婚同居和大学生非婚同居的概念的界定和法律制度的历史和现状。 第二章...With the ban on university students’ rights to marry and procreate being lifted, unmarried cohabitation among university students is becoming a thorny issue for university administration. Because of the lack of corresponding laws on the issue of unmarried cohabitation, problems created by unmarried cohabitation can not be solved. Failure to solve these problems in time leads to an increasing burde...学位：法学硕士院系专业：法学院法律系_民商法学(含劳动法学、社会保障法学)学号：X200512001

台湾杂志70年

中国台湾的杂志事业发展了近一个世纪,杂志事业的发展与台湾社会政治、经济、文化教育状况无不相关,从一段杂志史我们可以管窥台湾社会的发展史。台湾的杂志事业发展速度快、类型丰富多样、市场化程度高、国际合作密切,这些成功经验都值得国内业界借鉴学习。本文将台湾杂志事业史分期为报禁解除前的台湾杂志事业、报禁解除后的台湾杂志事业、新世纪的台湾杂志事业来展开论述

Exploration and practice on the teaching of designing experiments

分析了海洋物理学科课程体系的特点,论述开发设计性实验的必要性及可行性,讨论设计性实验的项目选择、教学方法和考核方式等。教学实践表明:设计性实验改变了原有各实验课程教学内容相对独立、缺乏系统的状态,加强了相关学科之间的联系,使学生建立了系统的概念,同时较大地提高了学生的实践能力和创新能力,达到了良好的教学效果。This paper analyzes the characteristics and specialties of the curriculum system setting methods of Marine Physics,argues the necessities and feasibilities of designing experiments exploitation,and also discusses the project selections,teaching methods,and assessment methods of designing experiment exploitation.The paper eventually comes to some conclusions that,designing experiments have changed the situations that previous experimental courses were unsystematic and relatively independent.Moreover,designing experiments strengthen the links between related disciplines,therefore, it let students all have a scientific concept of Marine Physics,and also significantly improve students' practical and innovative abilities.Thus,the teaching of designing experiments is effective with good results.厦门大学“985”专项经费资助项

ZTY-1型海床基水下系统状态监测仪的研制

国家863计划818主题资助项目818 11 26

Determination of amino acids in Alisma orientale by pre-column derivatization of RP-HPLC

目的　分析不同产地泽泻中氨基酸含量及其特征性。方法　采用邻苯二甲醛 (OPA)、氯甲酸芴甲酯(FMOC)联合柱前衍生RP HPLC测定泽泻中的 17种氨基酸。结果　氨基酸质量浓度与峰面积呈良好的线性关系 ,r均在 0 99以上。不同产地泽泻中氨基酸含量有一定差别。结论　该方法适宜泽泻药材中氨基酸含量的测定。Object To determine the contents of amino acids a nd study the characters of Alisma orientale (Sam. Juzep.) from d ifferent habitats. Methods The samples of A. orientale from thr ee different areas were derived with o-phthaldialdehyde (OPA) a nd 9-fluorenylmethyl chloroformate (FMOC) in pre-column, and separated by RP- HPLC. Seventeen kinds of amino aci ds were determined. Results The concentration of amino acids and peak areas achieve d a nice linear relations (r is higher than 0.99). There were differences of the amino acid in A. orientale from diffe rent habitats. Conclusion This method is suitable for the determination of am ino acid in A. orientale.国家自然科学基金 2 0 0 3重点项目 ( 2 0 2 3 5 0 2 0 ) ;; 福建省泽泻GAP研究项目 ( 2 0 0 2Y0 2 4

术前衰弱对胰腺癌患者胰十二指肠切除术后预后的影响

目的探讨术前衰弱指数对胰腺癌患者胰十二指肠切除术后预后的影响。方法对中山大学孙逸仙纪念医院435例行胰十二指肠切除术的胰腺癌患者的临床资料进行回顾性分析，统计患者术前衰弱情况，衰弱评估方法为衰弱（FRAIL）问卷。采用二元Logistics回归分析探讨衰弱的影响因素，采用Cox回归进行生存分析，按照人口学特征进行亚组分析并探讨衰弱对于胰腺癌患者胰十二指肠术后预后的影响。结果本研究共纳入患者435例，119例发生衰弱（占27.4%），316例无衰弱（占72.7%）。两组患者在年龄、BMI、ASA评分、术后输注红细胞、术后腹腔脓肿、CA199 、CA125、AFP、白细胞、中性粒细胞、HDL和疼痛程度等指标有显著性差异（P＜0.05）。高龄、ASA评分3级是衰弱的危险因素，HDL是衰弱的保护因素。非衰弱组的生存时间优于衰弱组。HDL是影响预后的独立保护因素，LDL是独立的危险因素。结论本研究表明术前衰弱状态是胰腺癌患者行胰十二指肠切除术后预后不良的重要预测因素。对于胰腺癌患者，术前应进行衰弱评估，并采取相应措施以改善患者的营养和代谢状况，以期提高术后生存率和生活质量

Determination of Glycyrrhizin and Liquiritin in Licorice(Glycyrrhiza) by HPLC Method

目的测定甘草中甘草酸和甘草苷的含量。方法采用反相高效液相色谱法,DENALIC18色谱柱(VYDAC238DE5415,120A,5μm,4.6 mm×150 mm),梯度洗脱,流动相A∶H2O,流动相B∶1.5%HAc-M eOH,流速1.0 m.lm in-1,检测波长在32.2 m in从330 nm换为252 nm,从35 m in换为330 nm,柱温40℃。结果甘草苷的浓度在10~50μg.m l-1之间与峰面积线性关系良好,平均回收率为99.97%,方法精密度RSD=0.12%(n=5),甘草酸的浓度在50~90μg.m-l1之间与峰面积线性关系良好,平均回收率为100.25%,方法精密度RSD=0.13%(n=5)。结论该方法可用于甘草中甘草酸和甘草苷的同时含量测定。ObjectiveTo determine the content of glycyrrhizin and liquiritin in licorice(Glycyrrhiza).MethodsRP-HPLC method was estabilished.The analytical column was DENALI C18(VYDAC 238DE5415,120A,5μm,4.6 mm×150 mm),and gradient elution with A:H_2O,B:1.5% HAc-MeOH as mobile phase.The flow-rate was 1.0 ml·min~(-1).The detection wavelength was from 330nm to 252 nm at the 32.2 min,and then returned to 252 nm at the 35 min.The column temperature was 40℃.ResultsThe content of liquiritin was good linear related to its peak area in range of 10～50 μg·ml~(-1),and the average recovery of the method was 99.97%,the RSD of precision test was 0.12%(n=5).The content of glycyrrhizin was good linear related to its peak area in range of 50～90 μg·ml~(-1),and the average recovery of the method was 100.25%,the RSD of precision test was(0.13)%(n=5).ConclusionThe method can be used for determing the content of glycyrrhizin and liquiritin in licorice at the same time.国家自然科学基金2003重点项目(No.20235020

Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2

大多数真核生物的体细胞是二倍体，即仅含有两组染色体，分别遗传自父本和母本。而一些特定组织如心脏、肝脏等就含有多倍体细胞，特别是肝脏组织含有较高比例的四、八倍体等多倍体细胞。肝脏是人体的重要解毒器官，同时酒精、肝炎病毒等毒性物质或毒性代谢物容易诱发肝细胞的基因突变，多倍体被认为有利于提供代偿性的正常基因来维持肝脏稳态。然而肝脏受损后，多倍体细胞将会受胁迫进行增殖，再生修复受损的肝组织。因此研究机体调控多倍体细胞产生及多倍体细胞进行细胞分裂的调控机理对于理解肝癌的发病机理和肝癌的治疗至关重要。Hippo信号通路在调节组织成体干细胞的分化和增殖，调控器官再生与尺寸大小中具有重要作用。深入研究发现, Hippo信号通路下游效应分子YAP通过AKT-SKP2信号促进二倍体细胞向多倍体转化及多倍体细胞的生长增殖。本项研究阐明了Hippo缺失及YAP激活促进多倍体细胞产生及增殖作为肝癌发生发展中的一个重要机制，为肝癌诊疗提供了新的策略。 周大旺，博士，厦门大学生命科学学院教授、副院长、国家杰出青年基金获得者。【Abstract】Polyploidy can lead to aneuploidy and tumorigenesis. Here, we report that the Hippo pathway effector Yap promotes the diploid-polyploid conversion and polyploid cell growth through the Akt-Skp2 axis. Yap strongly induces the acetyltransferase p300-mediated acetylation of the E3 ligase Skp2 via Akt signaling. Acetylated Skp2 is exclusively localized to the cytosol, which causes hyper-accumulation of the cyclin-dependent kinase inhibitor p27, leading to mitotic arrest and subsequently cell polyploidy. In addition, the pro-apoptotic factors FoxO1/3 are overly degraded by acetylated Skp2, resulting in polyploid cell division, genomic instability, and oncogenesis. importantly, the depletion or inactivation of Akt or Skp2 abrogated Hippo signal deficiency-induced liver tumorigenesis, indicating their epistatic interaction. Thus, we conclude that Hippo-Yap signaling suppresses cell polyploidy and oncogenesis through Skp2.该研究工作获得了国家自然科学基金委、国家重点基础研究发展计划(973)项目、青年千人计划和中央高校基本科研基金的资助。 The Yap (S127A) transgenic mice were kindly provided by Dr. Fernando Camargo from Harvard Medical School, Boston, MA. D.Z. and L.C. were supported by the National Natural Science Foundation of China (31625010,U1505224, and J1310027 to D.Z.; 81422018, U1405225, and 81372617 to L.C.; 81472229 to L.H.), the National Basic Research Program (973) of China (2015CB910502 to L.C.), the Fundamental Research Funds for the Central Universities of China-Xiamen University (20720140551 to L.C. and 2013121034 and 20720140537 to D.Z.)