Preparation, Characterization of Functionalized Polystyrene Microsphere and its Application in Biological Detection

流式微珠分析技术，又称液相芯片技术或悬浮芯片技术，是将溶液中的可溶性待测物质通过生物分子之间的特异性亲和反应结合在类似于细胞大小的经光学编码的微球体上，利用流式细胞仪对同一个微量样本中的多元待测组分同时进行快速定性、定量分析的新一代分子诊断技术平台。核酸杂交，免疫反应，酶学反应等都可以在同一个平台上实现。检测时，富集有待测物质的微球体逐一、顺次、高速地通过流式细胞仪的激光探测区，微球体本身带有的光学编码可用来区分不同的特异性结合，对待测物进行定性分析；微球体上报告分子所携带的荧光可对待测物进行定量分析。羧基功能性聚苯乙烯微球是流式微珠技术的重要载体，微球表面的羧基通过耦联剂活化之后可与蛋白、核...Microsphere-based array, also named as liquid chip or suspension array, is a powerful platform for multiplexed analysis of complex samples. Nucleic acid hybridization, immuno reaction, enzyme reaction can all be carried out on this platform. The suspension array is created by immobilizing capture reagents on the surfaces of encoded microspheres in conjunction with flow cytometric analysis. The arr...学位：理学硕士院系专业：化学化工学院化学系_分析化学学号：1912005130181

Synthesis and Application of Carboxyl Functionalized Polystyrene Microspheres

通讯作者：[email protected][中文文摘]采用分散聚合法合成了粒径可控、单分散的聚苯乙烯微球,考察了单体苯乙烯和引发剂偶氮二异丁腈的浓度以及稳定剂聚乙烯基吡咯烷酮的分子量对微球粒径的影响.采用后修饰法对合成的空白微球进行表面羧基功能性修饰,耦联单克隆抗体的免疫测试结果表明微球表面结合探针分子的能力达到国际同类产品水准.[英文文摘]Microsphere-based assay is a powerful platform to carry out biological detection.The carboxyl functionalized,micro-sized polystyrene microsphere is important carrier in the microsphere-based assay.Mono-dispersed polystyrene particles with the size range of 2.0 μm to 7.0 μm were synthesized by dispersion polymerization.The surface of microspheres was carboxyl functionalized by post-modification.It is demonstrated that the functionalized polystyrene microsphere can be well-coupled with monoclonal antibodies with comparable performance achieved as that of commercial carboxyl polystyrene microspheres (Spherotech Inc.).国家基础科学人才培养基金(J0630429);2007年度教育部新世纪优秀人才支持计划;教育部留学回国人员科研启动基金;2006年度福建省新世纪优秀人才支持计划资

面向智慧知识服务的科技文献大数据体系建设

&nbsp; [目的/意义]探索构建文献情报大数据知识资源体系,支撑面向多领域的智慧知识服务。[方法/过程]基于AI应用需求,借鉴业界经验,梳理现有资源体系的问题,从多层次多维度扩展资源体系;构建可靠数据处理流程和计算平台,支持高效数据采集和处理;研发智能化数据治理工具,实现知识资源的有效治理,确保提供高质量数据资源。[结果/结论]已初步形成覆盖多类型、多学科的科技文献大数据知识资源体系,构建完成高度自动化的数据采集治理流程,实施多重数据质量控制,积累数亿高质量数据,且为多个知识服务提供数据支撑。&nbsp;</p

Recent Advances in Big Defensins of Marine Invertebrates

Big defensins are cysteine-rich cationic antimicrobial peptides(AMPs)formed by serially connecting the N-terminal hydrophobic domain with the C-terminal beta-defensin-like domain.These domains have different antibacterial activities.Moreover,big defensins have conserved salt-stable antimicrobial activity and the N-terminal domain can drive the self-assembly of big defensins into nanonetworks that entrap and kill bacteria.So far,big defensins have been mainly found in the innate immune system of invertebrate animals and amphioxus that is a primitive chordate holding a key phylogenetic position bridging from invertebrates to vertebrates.Moreover,the C-terminal domain of big defensins has similar structure and bioactivity to the beta-defensin of vertebrates,which has aroused interest in investigating the biological evolution and function of defensins and the evolution of the immune system in species related to defensins.Based on the progress that has been made in the identification and characterization of big defensins in recent years,herein we present an integrated review of the molecular structures and evolution of big defensins,together with their antibacterial mechanism and expression regulation as well as their application prospects in the fields of aquaculture and aquatic product safety and in the development of new antimicrobial drugs.This review will provide a reference in the application of big defensins

碳化法纳米SiO_2表面结构研究

采用红外、热重、N2吸附等分析方法,研究了碳化法制备的纳米Si O2表面羟基、孔隙结构和吸油值等随陈化p H值、陈化时间及干燥温度的变化关系。实验结果表明,在不同陈化p H值下Si O2凝聚反应速率不同,陈化p H值由2升高到5,凝聚反应加快,颗粒表面羟基含量由1.54个/nm2减少到1.44个/nm2,平均孔径由11.51 nm增大到23.06 nm,吸油值由1.70 cm3/g升高到1.81 cm3/g;在不同陈化时间下Si O2颗粒间凝聚反应进程不同,陈化时间由10 min延长到90 min,颗粒表面羟基含量由1.48个/nm2减少到1.40个/nm2,平均孔径、吸油值变化不明显;在不同..

碳化法纳米SiO_2表面结构研究

采用红外、热重、N2吸附等分析方法,研究了碳化法制备的纳米Si O2表面羟基、孔隙结构和吸油值等随陈化p H值、陈化时间及干燥温度的变化关系。实验结果表明,在不同陈化p H值下Si O2凝聚反应速率不同,陈化p H值由2升高到5,凝聚反应加快,颗粒表面羟基含量由1.54个/nm2减少到1.44个/nm2,平均孔径由11.51 nm增大到23.06 nm,吸油值由1.70 cm3/g升高到1.81 cm3/g;在不同陈化时间下Si O2颗粒间凝聚反应进程不同,陈化时间由10 min延长到90 min,颗粒表面羟基含量由1.48个/nm2减少到1.40个/nm2,平均孔径、吸油值变化不明显;在不同...</p

碳化法纳米 SiO_2表面结构研究

采用红外、热重、N2吸附等分析方法,研究了碳化法制备的纳米Si O2表面羟基、孔隙结构和吸油值等随陈化p H值、陈化时间及干燥温度的变化关系。实验结果表明,在不同陈化p H值下Si O2凝聚反应速率不同,陈化p H值由2升高到5,凝聚反应加快,颗粒表面羟基含量由1.54个/nm2减少到1.44个/nm2,平均孔径由11.51 nm增大到23.06 nm,吸油值由1.70 cm3/g升高到1.81 cm3/g;在不同陈化时间下Si O2颗粒间凝聚反应进程不同,陈化时间由10 min延长到90 min,颗粒表面羟基含量由1.48个/nm2减少到1.40个/nm2,平均孔径、吸油值变化不明显;在不同..

金属盐催化剂对木粉半焦CO_2气化反应性的影响

在热币分析仪上进行了几种金属盐（K、Na、Ca、Mg、Fe）对杉木粉半焦CO2催化气化反应特性的研究；利用XRD和SEM技术对半焦样品的碳化程度、品相结构及表面形貌进行了表征。研究表明：5种金属盐均提高了半焦样品的反应活性；且反应活性指标显示其催化效果顺序为K〉Na〉Ca〉Fe〉Mg。XRD结果显示：Na和Ca在制焦过程中形成了明显的晶相结构；Mg增强了半焦中碳的有序化程度。SEM结果表明：5种金属盐均在半焦表面形成了“斑点”状的活化中心点且在碱金属半焦的部分表面观测到疏松片状结构