鉴定AP-4是在非激素依赖型前列腺癌中上调L-plastin表达的转录因子

【目的】鉴定AP-4是在非激素依赖型前列腺癌中调控L-plastin表达的转录因子，进一步阐明非激素依赖型前列腺癌的发病机制。【方法】在鉴定L-plastin启动子近3’端-216到1序列片段存在明显的转录活性的基础上，利用TFSEARCH软件分析该片段的序列，寻找可能调控L-plastin表达的非甾体激素类转录因子，并用凝胶滞后实验和超滞后实验证实结合于该片段的转录因子，利用PCR定点突变法构建删除该转录因子结合位点的重组子，并检测切除该片段序列后荧光素酶活性，研究该转录因子在非激素依赖型前列腺癌中调控L-plastin表达的作用。【结果】TFSEARCH软件分析表明在距转录起始点-199-194bp处含有AP-4转录因子结合位点CAGCTG，凝胶滞后实验和超滞后实验表明AP-4是结合于L-plastin启动子3’端序列CAGCTG的转录因子，删除AP-4结合位点后荧光素酶活性下降。【结论】AP-4是促进L-plastin在非激素依赖型前列腺癌中表达的重要调控因子

Separation and Purification of Naringin by Solid Phase Extraction Using Strong Anion Exchange Cartridge

作者简介：宁方刚(1986 —)，男，硕士研究生，研究方向为天然产物化学。E-mail：[email protected] * 通信作者：许建中(1954 —)，男，高级工程师，本科，研究方向为生物化工。E-mail：[email protected][中文文摘]目的:建立固相萃取分离纯化柚皮苷的方法。方法:以强阴离子交换(strong anion exchange,SAX)填料为吸附材料,利用柚皮苷与填料的功能基团之间的静电作用将柚皮苷吸附在填料上,考察洗脱液离子强度、洗脱液体积,洗脱流速对洗脱效果的影响。结果:选用5mL碳酸氢钠溶液(0.1mol/L,pH9.0)为洗脱液,以0.5mL/min的流速进行洗脱,回收率达到91%以上。结论:本方法简单、高效,选择性好,可为柚皮苷样品的纯化提供理论依据。 [英文文摘]Purpose: A solid-phase extraction procedure for the separation and purification of naringin(NG) was developed using strong anion exchange(SAX) cartridge.Method: Naringin was adsorbed onto the cartridge by electrostatic interaction between naringin and functional groups.Several variables affecting the elution performance of SAX,such as ionic strength of elution,elution volume and elution rate,were studied.Results: The application of 5 mL of sodium bicarbonate solution(0.1 mol/L,pH = 9.0) to elute SAX cartridge at a flow rate of 0.5 mL/min resulted in a recovery rate of 91%.Conclusions: The established method is simple and efficient.福建省科技计划重点项目(2010Y0035

Channel Estimation Based on Wiener Filter for Underwater Acoustic ofDM Communication Systems

为了进一步提高水声OfdM通信系统的性能,本文主要研究了水声OfdM通信系统中基于维纳滤波的信道估计算法.该算法结合了水声信道的特点,采用梳状导频图案,对传统的lS算法进行了改进.在完成计算机仿真的基础上,该算法还在实际的水声信道——厦门大学实验水池和厦门港浅海域进行实验.实验结果表明,该信道估计算法具有较好的精确度和稳定性,能够有效提高水声OfdM通信系统的性能.In order to improve the performance of the underwater acoustic orthogonal frequency division multiplexing(ofDM) communication system,a method of channel estimation for underwater acoustic ofDM communication systems based on wiener filter was presented.Considering the characteristics of the underwater acoustic channels,we use comb-pilots in our systems.The algorithm is an improvement of the traditional LS method.Computer simulation has been implemented and it has been tested in real underwater acoustic channels-the experimental pool in Xiamen University and in shallow water near Xiamen.The experimental result shows that the method presented here can improve the performance of the underwater acoustic ofDM communication system because of its validity and reliability.国家自然科学基金(60572106);厦门大学“985”二期信息创新平台项目资

Sound Pressure Measurement of Underwater Explosion and Its Effect on Marine Organism

简述水下爆破的物理现象．根据水下爆破所产生的声波的特点，介绍一种可行的声压测量方法．同时，通过对爆破点附近的一些养殖海洋生物的检测，讨论水下爆破对海洋生物的影响，为兼顾海洋开发与海洋渔业生产提供参考．Physics process of underwater explosion is introduced. Based on characters of sound wave produced by underwater explosion, a feasible sound pressure measurement method is proposed. In addition, with detection of some reared marine organism near the explosion site, underwater explosion ’s effect on marine organism is analyzed, which provide a reference for balancing the ocean exploitation and marine fishing

Research of Acoustic Wave's Effect and Impact on Marine Organism

本文研究了声波对某些海洋生物的影响与作用。实验表明 ,适当的声波剂量对海洋生物的作用所产生的声学生物效应是积极的 ,可促使生物体向有利的方向产生变异 ,而强度过大的声波对海洋生物有致伤甚至致死的效应In this paper, acoustic wave's effect and impact on marine organism is researched. Experiments show that while ultrasonic biology effect of moderate dose of acoustic wave plays an active role by promote marine organism's favorable variation, overdose acoustic wave can hurt or even kill marine organism.福建省自然科学基金资助项

长江江豚锌、铜、铅、镉和砷的摄入与累积

根据室内饲养的3头长江江豚(Neophocaena phocaenoides asiaeorientalis)食物鲫(Carassius auratus)中锌(Zn)、铜 (Cu)、铅(Pb)、镉(Cd)、砷(As)的浓度值和饲养记录,推算出了饲养条件下江豚这些微量元素每日及每周估计摄入 量的范围。必需元素的需求方面与世界卫生组织/联合国粮农组织(WHO/FAO)提出的人暂定每周耐受摄人量 (PTWI)相类似,可是毒性较强的元素Cd、As、Pb的摄入量大大高于人体的PTWI。对湖北天鹅洲故道收集到的一

东祁连山南坡现代花粉雨与植被

东祁连山南坡位于青藏高原、黄土高原和西北干旱区的过渡地带,植被类型具有多样性、复杂性和过渡性的特点,植被垂直分异十分明显.以该区主要植被类型下的表土花粉百分含量为数据源,采用DCA排序方法定量研究表土花粉的组合特征及其与环境的关系.结果表明:主要的植被类型可以由他们的表土花粉谱区分开来———杨桦林、云杉林、高寒灌丛、高寒草甸.花粉谱的变化也反映了植被随海拔高度和温度的变化.但杨桦花粉在林中的代表性有待进一步研究;氧化作用对花粉的破坏作用最大,花粉浓度随pH值升高呈指数递减,pH值76以上,花粉浓度迅速降低.国家重点基础研究发展规划项目“青藏高原形成演化及其环境、资源效应(G1998040800)”;;国家科技部重大基础研究前期研究专项(2003CCA01800);;国家自然科学(40171095)共同资