正交试验优选当归水提取工艺条件的研究

当归为多年生伞形科草本植物,主产于甘肃、云南、四川等地,根部入药,功能主治补血活血,调经止痛,润肠通便,其主要有效成分是阿魏酸。阿魏酸的化学名称为4-羟基-3-甲氧基肉桂酸,有顺式和反式两种,顺式为黄色油状物。反式为白色至微黄色结晶物,一般系指反式,分子量194.19,熔点174℃,微溶于冷水,可溶于热水。阿魏酸具有很强的抗氧化活性和抗菌活性,除了医药,在食品、化妆品、液晶、饲料添加剂等行业都有很广泛的用途

Isolation of Ferulic Acid from the Extractive Solution of Chinese Angelica(Danggui) Using Membrane Technology

研究了运用膜分离法从当归水浸取液中分离、纯化有效成分阿魏酸并进行浓缩的新工艺.首先,研究超滤法的可操作性.其次,对比研究超滤法和传统的醇沉法纯化当归水浸取液的处理效果,定量检测阿魏酸的回收率和杂质去除率.最后,为进一步提高阿魏酸浓度,分别选用纳滤膜和反渗透膜进行浓缩,并考察了操作压力和pH值对截留率的影响.实验结果表明:经过高速离心预处理后,超滤当归水提液的渗透通量变化不大,可用于当归水提液的纯化操作;超滤法的回收率和杂质去除率均高于醇沉法,说明新的超滤膜分离法在当归纯化效果方面优于醇沉法;纳滤膜对阿魏酸几乎没有截留效果,反渗透适用于浓缩阿魏酸,提高pH值可以提高截留率,本实验条件下pH=9~10,压力2.5 MPa时反渗透获得最高的截留率.A new process membrane to separate,purify and concentrate ferulic acid from the extractive solution of Chinese Angelica(Danggui),which is just the effective component of this traditional Chinese herb was reported in this paper.At first,the permeate flux experiment gave a quite good performance and indicated the feasibility of separation and recovery of ferulic acid from the pretreated solution by using ultrafiltration(UF).And then,UF and traditional alcohol precipitation method were adopted to purify ferulic acid respectively.Both the recovery ratio of ferulic acid and the removal ratio of impurity verified that UF technology was superior to the alcohol precipitation method.Moreover,in order to condense solution for industrial application of ferulic acid,nanofiltration(NF) and reverseosmosise(RO) were tested with consideration of the influence of operation pressure and pH value to the interception ratio of ferulic acid respectively.Trials showed that RO,but not NF had a good seizing effect on the ferulic acid with a practicable flux,and that the RO interception ratio increased with rising pH value in feed,meanwhile varied in a non-linear proportional relationship to the operation pressure.In our research,the optimal RO operation condition was:p=2.5 MPa,pH=9~10.To sum up,this new membrane technics involving UF and RO could be successfully developed to obtain ferulic acid from the water extraction of Chinese Angelica(Danggui) and avail mass production