Analysis of genetic variability of Castanopsis hystrix by rbcL gene sequences

通过PCR扩增得到10个红锥品系的rbcL基因全序列,均为1.513 kb.核苷酸序列比对结果表明,在256-1 455 bp区间10个红锥品系共有16处碱基发生变异,变异率为1%,其中在306 bp和634 bp处10个红锥品系较同属对照castanopsis lucida均发生相同的变异,可以看作属内进化信息碱基.对表达的氨基酸序列进行比对分析,发现16处碱基变异共引起8处氨基酸序列变异.The rbcL gene sequences of 10 assassin of Castanopsis lucida were analyzed by PCR,and sixteen mutations in 256-1 455 bp were found,the mutation ratio was 1 %.Compared to Castanopsis lucida,between 306 bp and 634 bp,they had two characteristic mutations of their own,so it was different from Castanopsis lucida.Compared to Castanopsis lucida,8 amino acid variations were found,caused by 16 point mutations.广西林业科学研究院国家林业局中南速生材繁育重点实验室开放基金课题;; 兰州理工大学博士基金(SB08200602);; 甘肃省教育厅研究生导师基金( 0703-11)

Analyzing Effects of Different Reed Patches on Contents of N and P in Water Column of the Lake Wuliangsuhai

农田退水和废污水的排入导致乌梁素海湿地环境发生改变,富营养化的程度日益严重.本文研究了乌梁素海不同芦苇斑块对总氮和总磷的吸收特征,发现底泥主要富集磷、湖水氮含量比较高,不同芦苇斑块对N、P的影响程度不同,长斑块中部芦苇吸收N最强,近岸芦苇吸收P最强,而短斑块近湖心部分芦苇吸收N、P最强,主要依赖不同位置芦苇斑块的生长状态