Differential Host Gene Expression during the Infection with Three Alveolar Echinococcus from Northeast Inner Mongolia, China

唐崇惕等对我国内蒙古东北部呼伦贝尔草原泡状棘球蚴的病原情况进行调查时，在沙狐（Vulpescorsac）体内发现多房棘球绦虫的成虫，在布氏田鼠（Microtusbrandti）体内发现了泡状棘球蚴。随后的研究发现，流行于此地区的泡状棘球蚴病原应包含独立的三种，分别为：欧洲的多房棘球绦虫（Echinococcusmultilocularis），西伯利亚棘球绦虫（Echinococcussibiricensis）以及苏俄棘球绦虫（Echinococcusrussicensis）。 本论文主要对这三种泡状棘球绦虫引起的宿主体内基因差异表达变化进行研究，包括以下三个部分： 1.三种泡状棘球绦虫棘球...At the end of last centry, Tang Chongti et al found there were three types of alveolar echinococcus in the Northeast Inner Mongolia, China. Subsequent studies indicated that they should be regarded as independent species: Echinococcus multilocularis, Echinococcus sibiricensis and Echinococcus russicensis. In this dissertattion, the differential host gene expression during the infection with thre...学位：理学博士院系专业：生命科学学院生物学系_动物学学号：2012005140311

An Experimental Study on the Anticoagulant Effect of the Extract from Tick on Rabbits and Human Plasma

通过体外试验,观察牛蜱粗提物对家兔及人血浆的抗凝血效果.通过测定与比较加入牛蜱粗提物与未加牛蜱粗提物的兔血浆和人血浆的血浆复钙时间(RT)、凝血酶原时间(PT)、白陶土部分凝血酶激活时间(KPTT),发现牛蜱粗提物能使兔血浆及人血浆RT、PT、KPTT均显著延长,且其作用随着牛蜱粗提物用量的增大而增强.实验结果显示牛蜱粗提物具有明显的抗凝作用.Ticks are common ectoparasites of human and domestic animal and they are regarded as important vectors of zoonose disease due to their ability of transmitting a lot of pathogens to animal and people.As ticks obtain their nutrition from host by sucking their blood,their saliva must contain components to prevent the coagulation of the host blood. To investigate the anticoagulant effect of the extract from the tick on rabbits and human plasma,recalcification time(RT),prothrombin time(PT)and activated kaolin partial thromboplastin time(KPTT) were detected in vitro following the tick extract being added into the plasma of rabbits and human.Compare to the control group in the experiments,the results of RT,PT and KPTT of the test group which the tick extract was added in were shown prolonged significantly.And the anticoagulant effects were dosage dependent.The data showed that the tick extract has prominent anticoagulant effects on rabbits and human plasma.福建省青年科技人才创新项目(2002J051)资

Morphological and structural characterization of haemocytes of Oncomelania hupensis (Gastropoda: Pomatiopsidae)

Natural Science Foundation of the People's Republic of China [31270938, 81000740]; Chinese Scholarship Council (CSC) of the People's Republic of ChinaOncomelania hupensis, an intermediate host of Schistosoma japonicum, remains an important public health problem. Haemocytes play an important role in an innate immunity defense system of O. hupensis and require more research. The present study provides information concerning the morphological and functional basis of defense cells of O. hupensis observed through optical microscopy and electron microscopy [scanning electron microscopy (SEM) and transmission electron microscopy (TEM)]. In this study, two types of haemocyte cell categories, type I (macrophage-like) and type II (lymphocyte-like), were distinguished according to shape, size, surface structure, internal structure, functions, structure of cytoplasm and the processes of spike-like filopodia. The results showed that type II cells generally were smaller than type I cells. In addition, only type I cells had the ability to adhere to sheep red blood cells (SRBC) after 60 minutes of incubation. The adherence to SRBC of type I cells represents adhesion activity of these cells to foreign particles or parasites. These results warrant further investigation to better understand the function of O. hupensis haemocytes. The present study confirms several previous findings concerning haemocytes of O. hupensis and opens the door for further research into the function and structure of O. hupensis haemocytes

FTIR study of the oxygen reduction reactions on Sm0.5Sr0.5CoO3

Conference Name:Asia-Pacific Conference on Surface Science and Engineering. Conference Address: Hong Kong, PEOPLES R CHINA. Time:DEC 19-21, 2006.The oxygen reduction on solid oxide fuel cell cathode electrode, Sm0.5Sr0.5CoO3, was studied by using emission Fourier transform infrared spectroscopy (FTIR). Superoxide (O-2) species was detected with appearance of an IR peak at wavenumber about 1124 cm(-1). Moreover, we interpreted for the first time that there would be a close relationship between the baseline shift of IR spectra and the oxygen vacancy concentration (OVC) of the samples. The downward baseline shift of IR spectra is due to the decrease of OVC in the bulk, while, the increase of the OVC in the bulk would cause the upward baseline shift

典型农牧区现代农业发展思路研究——以内蒙古包头市土默特右旗为例

中国农业正处于由传统农业向现代农业转变的关键时期。发展现代农业,是解决"三农"问题的根本出路。农牧交错区是环境变化敏感、生态环境脆弱的区域,也是国家农业发展和治理的重点区域。内蒙古自治区土默特右旗(简称土右旗)是典型的农牧地区。通过对土右旗基础现状的分析,总结现代农业发展的优势、劣势、机遇、挑战,研究现代农业发展的核心战略问题,提出土右旗现代农业发展思路,进而归纳出农牧区现代农业发展的整体思路,为中国农牧地区的可持续发展提供理论借鉴

一氧化氮在植物发育及植物–微生物互作中的作用机制研究进展

一氧化氮(NO)作为高活性信号分子,是调控植物生长发育的关键因子。NO可提高植物对非生物胁迫及生物胁迫的抗性,增强植物的免疫能力。最新的研究表明,NO在植物根系与微生物的互作过程中发挥着重要作用,NO能够促进植物根系与根瘤菌及丛枝菌根真菌形成共生体,从而提高植物对土壤氮磷养分的获取。NO作为信号物质调控植物对生物胁迫和非生物胁迫抗性的主要机制有:1) NO与活性氧系统互作,调节活性氧的水平,缓解氧化应激反应对植物的伤害;2) NO通过蛋白质的翻译后修饰,对植物免疫及抗逆过程进行调节;3) NO与多种植物激素互作,参与激素对植物生长发育的调节过程。而且NO可促进共生体的形成及发育相关基因表达,抑制免疫基因表达,通过NO与植物球蛋白(phytoglobin)的循环维持共生体的氧化还原水平及能量状态,从而促进植物–微生物共生关系。以往关于NO的研究主要集中在前3个方面,有关NO在植物–微生物互作中的作用机制的研究较少,NO参与植物–微生物互作机制的研究亟待加强。揭示NO增强植物抗逆性及其调节根系发育的机制,深入探究NO调控植物–微生物互作的机理,对于提高集约化作物生产体系中养分利用效率和作物生产力具有重要的理论与实践意义

The effect of tributyltin chloride on Caenorhabditis elegans germline is mediated by a conserved DNA damage checkpoint pathway

Fundamental Research Funds for the Central Universities of People's Republic of China [2010121091]; Programme of Introducing Talents of Discipline to Universities [B06016]; National Natural Science Foundation of China [81000740, 81071384, 81171596]; NIH National Center for Research Resource, USATributyltin (TBT), one of the environmental pollutants, has been shown to impact the reproduction of animals. However, due to the lack of appropriate animal model, analysis of the affected molecular pathways in germ cells is lagging and has been particularly challenging. In the present study, we investigated the effects of tributyltin chloride (TBTCL) on the nematode Caenorhabditis elegans germline. We show that exposure of C. elegans to TBTCL causes significantly elevated level of sterility and embryonic lethality. TBTCL exposure results in an increased number of meiotic DNA double-strand breaks in germ cells, subsequently leading to activated DNA damage checkpoint. Exposing C. elegans to TBTCL causes dose-and time-dependent germline apoptosis. This apoptotic response was blocked in loss-of-function mutants of hus-1 (op241), mrt-2 (e2663) and p53/cep-1 (gk138), indicating that checkpoints and p53 are essential for mediating TBTCL-induced germ cell apoptosis. Moreover, TBTCL exposure can inhibit germ cell proliferation, which is also mediated by the conserved checkpoint pathway. We thereby propose that TBT exhibits its effects on the germline by inducing DNA damage and impaired maintenance of genomic integrity. Index Descriptors and Abbreviations: TBTCL, tributyltin chloride; C. elegans, Caenorhabditis elegans; NGM, nematode growth medium; DMSO, dimethyl sulfoxide; DAPI, 4', 6-diamidino-2-phenylindole; DSBs, DNA double-strand breaks. (C) 2014 The Authors. Published by Elsevier Ireland Ltd. All rights reserved

多基因风险评分的构建及其在卒中领域的应用 Construction and Application of Polygenic Risk Score in Stroke

卒中是一种由遗传和环境因素共同作用引起的复杂疾病，人类遗传学的发展为卒中的个体化预防和治疗提供了新的机会。多基因风险评分是根据个体的基因型和相关的全基因组关联研究数据，对一种性状或疾病的遗传倾向进行估计，是遗传学研究中全基因组关联研究的补充方法。多基因风险评分可以对患者进行风险分层以进行个性化管理，从而有助于卒中等复杂疾病的精准预测和防控。基于此，本篇综述阐述了多基因风险评分的构建及其在卒中研究中的应用情况，以期为国内相关研究提供思路。 Abstract: Stroke is a complex disease caused by genetic and environmental factors. The development of human genetics had provided new opportunities for individualized prevention and treatment of stroke. Polygenic risk score (PRS) is an estimate of an individual's genetic liability to a trait or disease, calculated according to their genotype profile and relevant genome-wide association study (GWAS) data. PRS is a complementary method to GWAS in genetic research. PRS can stratify the risk of patients for personalized management, thus contributing to accurate prediction, prevention, and control of complex diseases such as stroke. Based on this, this review describes the development of PRS and its application in stroke research, aiming to provide insights for relevant domestic studies

Echinococcus multilocularis: Proteomic analysis of the protoscoleces by two-dimensional electrophoresis and mass spectrometry

Echinococcus multilocularis is an important parasite that causes human alveolar echinococcosis. Identification and characterization of the proteins encoded by E. multilocularis metacestode might help to understand the complexity of the parasites and their interactions with the host, and to identify new candidates for immunodiagnosis and vaccine development. Here we present a proteomic analysis of E. multilocularis protoscolex (PSC) proteins. The proteins were resolved by 2-DE (pH range 3.5-10), followed by MALDI-TOF MS analysis. Fourteen known Echinococcus proteins were identified, including cytoskeletal proteins, heat shock proteins, metabolic enzymes, 14-3-3 protein, antigen P-29 and calreticulin. To construct a systematic reference map of the immunogenic proteins from E. multilocularis PSC, immunoblot analysis of PSC 2-DE maps was performed. Over 50 proteins spots were detected on immunoblots as antigens and 15 of them were defined. The results showed that cytoskeletal proteins and heat shock proteins were immunodominant antigens in alveolar echinococcosis. (C) 2009 Elsevier Inc. All rights reserved.Natural Science Fund of China [30471514]; Program for New Century Excellent Talents in Fujian Province University ; Project of Innovation Foundation of Xiamen Universit

通辽市森林、草原、沙地、湿地碳汇产业规划研究

全球和区域碳汇是全球变化研究和生态学研究的核心内容之一。为应对气候变化,世界各国各组织都在积极行动。本研究深入剖析了通辽市的植物资源、宜林区域及发展碳汇工程的基础条件,提出碳汇产业的发展思路及原则,确定碳汇产业的建设目标、碳汇工程的技术路线及技术指标,最后对发展的综合效益进行评价,制定产业发展的保障措施,进而形成完整的碳汇产业发展规划思路,为生态脆弱的欠发达民族地区的碳汇产业发展提供借鉴