Circular RNA CircHIPK3 Promotes NCI-H1299 and NCI-H2170 Cell Proliferation through miR-379 and its Target IGF1

背景与目的已有的研究证明:环状RNA是一类在哺乳动物中普遍存在的具有稳定闭合环状结构的内源性RNA分子。环状RNA; circHIPK3(circular RNA HIPK3, circHIPK3)在肝细胞癌(hepatocellular carcinoma,; HCC)中表达水平较高,促进肝癌细胞生长。但是其在非小细胞肺癌(non-small cell lung cancer,; NSCLC)中的作用及其调控机制尚无文献报道。本研究拟探讨环状RNA; circHIPK3对NSCLC细胞系NCI-H1299和NCI-H2170细胞增殖的影响,并进一步研究其调控的分子机制。方法Real-time; PCR法检测circHIPK3在NSCLC各细胞系中的表达水平。CCK-8实验和克隆形成实验检测过量表达和干扰circHIPK3对细胞增殖的影响; 。双荧光素酶报告基因实验分别检验miR-379与circHIPK3及miR-379与IGF1 mRNA的结合情况。Western; blot和ELISA检测细胞内外的IGF1蛋白表达水平。结果环状RNA; circHIPK3在6株NSCLC细胞株中均有表达,其中H1299表达最低,H2170表达最高,通过转染过表达的circHIPK3可显著促进NC; I-H1299细胞增殖,干扰circHIPK3可显著抑制NCI-H2170细胞增殖。miR-379可与circHIPK3及IGF1; mRNA直接结合。过表达circHIPK3导致IGF1表达量上调,干扰circHIPK3能够下调IGF1表达水平,转入miR-379; mimics恢复了circHIPK3稳转细胞株IGF1表达水平的上调及细胞增殖表型。结论环状RNA; circHIPK3在NSCLC细胞系NCI-H1299及NCI-H2170中可通过miR-379调控IGF1表达促进细胞增殖,环状RNA; circHIPK3可能成为非小细胞肺癌治疗的新靶点。Background and objective It has been proven that the circular RNA,; possessing a stable covalently closed continuous loop, is a type of RNA; molecule which is expressed widespread in mammals. The circular RNA; circHIPK3 is abundantly expressed in hepatocellular carcinoma (HCC) and; promotes tumourgenesis. However, a role for circHIPK3 has not been; systematically examined in non-small cell lung cancer (NSCLC). In this; study, we investigated whether circHIPK3 has an effect on cell; proliferation in the NSCLC cell lines NCI-H1299 and NCI-H2170 and the; underlying molecular mechanisms. Methods The expression of circHIPK3 was; measured by real-time PCR in NSCLC cell lines. Nuclear mass separation; experiment was used to detect the location of circHIPK3 in cells. The; gain and loss function experiments were used to examine the; proliferation of NCI-H1299 and NCI-H2170 cells by Cell Counting Kit-8; (CCK-8) and the colony formation assays. Then, circHIPK3 was cloned into; the downstream of the luciferase reporter gene which activity was; detected to verify whether miR-379 could bind with circHIPK3 or IGF1; mRNA. The protein level of IGF1 was detected by Western blot and ELISA; in circHIPK3 overexpressed/knock-down NCI-H1299 and NCI-H2170 cells.; Results CircHIPK3 was generally expressed in six kinds of NSCLC cells; lines we detected, and the expression level was highest in H2170 and; lowest in H1299. Overexpression of circHIPK3 obviously promoted; NCI-H1299 cell proliferation and knock-down of circHIPK3 inhibited; NCI-H2170 cell proliferation. In the luciferase assay, miR-379 was; observed to sequester circHIPK3 and IGF1 mRNA with potential binding; sites. Furthermore, we found that the overexpression of circHIPK3 could; increase the expression levels of IGF1 and knockdown reduced it.; Moreover, up-regulation of miR-379 rescued the phenotype induced by; overexpression of circHIPK3. Conclusion CircHIPK3 could promote cell; proliferation by a circHIPK3/miR-379 pathway in NCI-H1299 and NCI-H2170; cells and might be a potential tumor biomarker for NSCLC.国家自然科学基金项

Stress Concentration Analysis for Bolt and Nut Joint Using Finite Element Method

ボルト締結体は長い使用歴史を持っているにもかかわらず、振動等の外力によるねじ谷底での応力集中に起因した重大事故が現在でも発生している。すでに応力集中の対策を施したボルト・ナットが幾つか開発され市販されているが、その改善効果に関するメカニズムは未だ不明な点が多い。本研究では、まず有限要素法によってボルト・ナットの締結状態をシミュレーションし、高精度の有限要素解析法を求め、ボルトねじ山谷底の応力集中に影響する因子を明らかにした。それを基に、ボルト・ナット締結体に最適な形状設計を適用することにより、最大20%の応力緩和効果が得られた

Antifreeze activities of fungi and sea ice diatoms from Antarcica

第32回極域生物シンポジウムポスター発

Expression and characterization of recombinant antifreeze proteins from cold-adapted microorganisms

第32回極域生物シンポジウムポスター発

日・中・シンガポールの職業会計人育成制度の比較研究

This paper aims to compare the status of the development of the education program of the accounting profession in Japan, China and Singapore. And this study is to compare the educational program for the qualification of Certified Public Accountants.International Education Standards (IES) has been published by worldwide organization of accounting profession IFAC, and every countries should follow the rule of IES, especially when education programs are set in non-European countries. When the international education standards (IES) published by the International Federation of Accountants (IFAC) is considered to be standard, the current state education programs in three countries are examined.Among three countries, Singapore’s education program contain almost the same content and quality of IES. On the other hand, education programs of Japan and China have differences in the following three points; (1) required skills are not show clearly, (2) required program to participate and (3) the period of work experience is short one year (3 years vs 2 years)

Application of Fuzzy Comprehensive Evaluation to Human Resource Value Accounting Measurement

针对人力资源价值的许多特性是货币无法表现的这一特征，分析了影响人力资源的个人、组织和社会诸方面因素，把模糊综合评价引入到人力资源价值的非货币性方面进行计量、定性研究，建立了评价指标体系．为人力资源的考核提供了依据．On the basis of the many characteristics of human resource value that monetary index couldn't show, the paper analysis the factors of individual. organization and society etc which affect human resource. We introduce fuzzy comprehensive evaluative to the non-monetary aspects of human resource for fuzzy measurement, quantitative research, and set up evaluation index system. It offers the ideas to solve the problems and resource for the evaluation of human resource