战后台湾民间宗教研究的回顾与前瞻

在民间宗教成为专业性学科话语的半个世纪里,研究范式已是屡经更迭。就台湾民间宗教研究而言,它始终与外部的现实环境相交涉并随之变迁。战后初期,有两个重要的变化值得我们注意:一是自1950年代起,政府权力开始在宗教领域申张;其二,1949年后,被称为“新兴宗教“的大陆民间教派开始传入,形成了台湾民间宗教蓬勃兴盛的局面。1970年代,由冈田谦所提出的“祭祀圈“理论被引入浊水、大溪两大区域的研究中,其后祭祀圈、信仰圈和村庄庙讨论盛极一时。1980年代后,民间教派和鸾堂成为新的议题。台湾的民间宗教及信仰问题长期未受到学界主流的重视,而这一领域的拓展仍有待于新的理论。教育部哲学社会科学重大攻关课题“民间信仰与中国社会研究”(04JZD00030)的支

Universal primer PCR with SSCP and RFLP for identification of fish disease pathogens

采用通用引物PCR(UPPCR)、PCR RFLP、PCR SSCP技术 ,研究快速鉴别鱼病病原菌的分子生物学诊断技术。结果发现 ,采用细菌 16SrRNA基因保守区特异性引物 ,以嗜水气单胞菌、鲁克氏耶尔森菌、鳗弧菌、柱状曲挠杆菌、乙型链球菌、荧光假单胞菌等部分常见鱼病病原菌为对象 ,可以建立一种UPPCR技术。该技术能在保证实验条件不变的基础上 ,检出上述所有细菌 ,并还可检出大肠杆菌和双歧杆菌等非鱼病病原菌。并且认为 ,该法与SSCP配合即采用UPPCR SSCP技术能较好地鉴别被检菌而用于鱼病病原菌的快速诊断。A universal primer PCR(UPPCR), PCR RFLP and PCR SSCP had been screened for a molecular biological method that permitted the rapid identification of fish pathogens among them. The results showed that a universal primer PCR technology was available with specific primers from conserved regions of bacterial 16S ribosomal RNA genes. The bacteria tested included some common causative agents of fish diseases such as Aeromonas hydrophila, Yersinia ruckeri, Vibrio anguillarum, Flexibacter columnaris, beta Streptococcus, Pseudomonas fluorescens. The approach allowed the bacteria above and Escherichia coli and Bifidobacterium catenulatum detectable without any alterations of the experiment conditions. However, this study also found that the identification of species of bacteria tested depended on the combination of UPPCR and SSCP(PPCR SSCP), which was better than that of UPPCR and RFLP(UPPCR RFLP) and made rapid diagnosis of fish disease pathogens possible.国家自然科学基金!资助项目 (39770 5 85 );; IFS基金!(ImmunecomplexesinfreshfishinfectedFlexibactercolumnaris)资助项

Inhibitory effect of anti-human NRP-1 monoclonal antibody on hepatocellular carcinoma cell line HepG2 and its mechanism in vitro

目的研究抗人神经纤毛蛋白-1(Neuropilin1,NRP-1)单克隆抗体对肝癌Hep G2细胞的生长抑制作用及其机制。方法小鼠腹水法制备抗NRP-1单克隆抗体(NRP-1 m Ab)并用r Protein A亲和柱纯化抗体,间接ELISA检测抗体的滴度水平。Western blot检测NRP-1 m Ab对Hep G2细胞的特异性,细胞免疫荧光和流式细胞术检测NRP-1蛋白在肝癌细胞株Hep G2上的表达,MTT法检测NRP-1 m Ab对Hep G2的生长抑制作用,Western blot检测ERK1/2、P-ERK1/2、Akt、P-Akt蛋白的表达水平。结果 SDS-PAGE和间接ELISA检测纯化的NRP-1 m Ab纯度为95%以上,效价为1×10~(-6);Western blot检测结果显示NRP-1 m Ab可与Hep G2细胞膜上的NRP-1蛋白特异性结合。细胞免疫荧光染色结果显示NRP-1定位于Hep G2细胞膜,流式细胞术结果显示NRP-1蛋白在Hep G2细胞上表达水平较高;MTT法检测结果显示NRP-1 m Ab对Hep G2细胞有生长抑制作用。Western blot检测到在不同浓度NRP-1 m Ab作用下,Hep G2细胞裂解液P-ERK1/2、P-Akt蛋白的条带信号逐渐减弱。结论纯化的NRP-1m Ab能抑制Hep G2细胞的生长,其抑制作用是通过EGF和HGF信号通路实现的。The aim of the experimental is to investigate the inhibitory effect of anti-human nerve cilia protein1(Neuropilin-1,NRP-1) monoclonal antibody(NRP-1 mAb) on hepatocellular carcinoma cell line HepG2 and its mechanism in vitro.Anti-human NRP-1 monoclonal antibody(NRP-1 mAb) was prepared from mouse ascites and purified by rProteinA affinity column assay.The titer of antibody was determined using indirect ELISA assay;the characteristic of NRP-1 mAb binding to NRP-1 was determined using Western blotting;the expression of NRP-1protein in hepatocellular carcinoma cell line HepG2 was determined using immunofluorescence assay and flow cytometry assay.Growth inhibition of HepG2 cells treated with different concentrations of NRP-1 mAb was determined using MTT assay,while Western blotting was used to detect the expression levels of ERK1/2,P-ERK1/2,Akt and P-Akt proteins.The results of SDS-PAGE and indirect ELISA showed that the purity of purified NRP-1mAb was more than 95%and the titer was 1×10~(-6).Western blotting analysis suggested that NRP-1 mAb could bind specifically to NRP-1 on HepG2 cell;immunofluorescence staining showed that NRP-1 was located in the membrane of HepG2 cells.Flow cytometry analysis showed that the expression level of NRP-1 on HepG2 cell was relatively high.Western blotting analysis suggested that P-ERK1/2 and P-Akt expression levels were down-regulated after having incubated HepG2 cells with different concentrations of NRP-1 mAb.In conclusion,NRP-1 mAb could inhibit the growth of HepG2 cells(P<0.05),and its inhibitory effect is achieved by reducing the P-ERK1/2 and P-Akt expression.南京军区医学科技创新项目(12MA061,15MS104

抗胸腺细胞血清性肾炎模型大鼠肾小球中C5b-9的沉积及NO、TNFα含量分析

探讨抗胸腺细胞血清性肾炎 (ATSN)大鼠肾小球内C5b -9复合物的沉积与某些炎症介质和细胞因子如 :一氧化氮 (NO)和肿瘤坏死因子α(TNFα)的含量情况。方法 :大鼠一次性静脉注射抗胸腺细胞抗血清(ATS)建立ATSN模型 ,定期对ATSN大鼠肾小球中的补体C5b -9复合物进行免疫组化染色定位、显微图像扫描半定量分析 ;并对有C5b -9包绕的肾小球系膜细胞 (MC)进行计数。测定ATSN大鼠肾中诱生型NO合酶 (iNOS)mRNA的表达、尿液中NO的代谢产物 (NO-2 /NO-3 )及TNFα的排泄量。结果 :ATSN模型大鼠肾小球MC先溶解坏死后继发增生 ,病变早期 (溶解时相 )补体C5b -9复合物主要定位于肾小球系膜区及MC表面 ;随着病程的进展 ,被C5b -9包绕的MC逐渐减少 ,病程初期ATSN大鼠肾小球MC有明显的iNOSmRNA表达 ,尿液中NO-2 /NO-3 和TNFα的排泄量也明显增加。在ATSN病变的增生阶段 (一般 7d后 ) ,上述指标的变化逐渐趋缓。结论 :ATSN模型大鼠肾小球中MC逐渐溶解与补体C5b -9沉积及NO和TNFα的合成与释放有一定关

LARGE-SCALE EXPOSITION PLANNING AND MANAGEMENT OPTIMIZATION BASED ON VISITORS' BEHAVIOR SIMULATION:A CASE STUDY of QINGDAO INTERNATIONAL HORTICULTURAL EXPOSITION 2014

2014年青岛世园会参观人次众多,需要对参观者行为进行模拟分析,以保证活动安全,提升游客体验。研究提出了通过行为模拟支持规划评价、预警及对策的技术框架。利用路径调查与陈述偏好调查获得参观者行为数据并建立模型,进而在多个情景下对个体行游轨迹进行了模拟仿真,分析预测了参观者排队、密度等参数的时空分布情况。在针对不同类型的评价对象提出各自评价指标的基础上,从模拟结果中辨识可能存在的问题,并从规划与管理两方面提出预警及优化对策。研究证明了对大型展会活动开展参观者行为模拟的技术框架的可行性,其结果对本次世园会具有一定的实践指导意义。It is necessary to simulate visitors' behavior in Qingdao International Horticultural Exposition 2014 for security and high-quality visitor experience due to the huge estimated pedestrian volume.This paper proposes a technical framework in purpose of supporting the assessment, early warning as well as countermeasures based on behavior simulation.Visitors' activity data are collected through a route choice survey and the informants' reported preference, based on which the behavior models are estimated.Visitors' trajectories under several scenarios are simulated, together with the spatio-temporal distributions of visitors' density and queuing behaviors.Criteria are proposed to evaluate each simulated object, based on which indexes are set up, used to identify problems that are likely to happen in the real exposition.In the end, the paper proposes early warnings and countermeasures of both planning and administration.The simulation framework is demonstrated to be feasible and valuable for the exposition.国家自然科学基金项目“基于行为分析的商业综合体空间优化方法研究”的部分成

3个地区西施舌的ITS-1基因片段序列分析

首次检测了西施舌的ITS-1基因序列片段.并利用ITS-1序列作为遗传标记分析了来自3个不同地区(江苏、福建长乐、福建深沪湾)的西施舌的遗传变异情况.地理位置较近的福建长乐和福建深沪湾的西施舌遗传距离较小,江苏和福建(长乐漳港和深沪)西施舌的遗传距离较大.基于ITS-1序列分析,作为品质突出的福建长乐西施舌和其他地区的西施舌相比,并未形成一个区别于其他地区西施舌的一个独特的种.还基于ITS-1序列分析了西施舌在蛤蜊科和双壳贝类中的分子系统进化地位

Effect of rich organic matter on Paraprionospio pinnata (Polychaeta:Spionidae) on Futian tidal flat in Shenzhen Bay

根据1999年1月至2002年10月在深圳湾福田潮滩A、D、E三个断面获得的大型底栖动物奇异稚齿虫(Paraprionospiopinnata)和有机质数据,分析了深圳湾福田潮滩奇异稚齿虫对有机质含量的效应特征。结果表明,深圳湾福田潮滩奇异稚齿虫有明显的季节变化,即3、4月密度高,形成全年的高峰期,9、10月密度低,形成全年低谷期。奇异稚齿虫密度随着有机质含量的增加逐渐降低,前者密度和生物量大小是:A>D>E,而有机质大小也是:AD>E.The distribution of organic matter content is also A<D<E.There is negative correlation between density of P.pinnata and organic matter content.The higher organic matter is,the lower density and biomass of P.pinnata are

光动力学方法（PDT）治疗多形性腺瘤恶变一例报告

多形性腺瘤恶变采用PdT治疗国内尚未见报道。我科1988年治愈一例，存活至今已7年未复发。患者张xx，女，30岁，1985年右上唇颊沟长一肿物，在某地区医院手术切除三次复发。1987年7月21日我科收住院。入院检查：全身情况营养较差，消瘦、肝功gPT..

圣·约翰草提取物片治疗抑郁障碍专家共识

结合循证医学证据,本文归纳圣·约翰草提取物片治疗抑郁障碍的专家共识