Immunohistochemical Study on 5-Hydroxytryptamine Immunoreactive Cells in the Alimentary Tract of Bufo melanostictus and Rana nigromaculata

对黑眶蟾蜍(Bufomelanostictus)和黑斑蛙(Rananigromaculata)消化道5-羟色胺免疫活性细胞的形态、分布进行了免疫组织化学定位。5-羟色胺免疫活性细胞在黑眶蟾蜍和黑斑蛙消化道各段中均有分布,分布密度均呈升-降-升-降的波浪式分布特点,二者在幽门部和回肠都有个分布的高峰值。黑眶蟾蜍回肠最高,空肠、幽门部次之,十二指肠、直肠最低;黑斑蛙幽门部最高,回肠、空肠次之,食道、贲门部、直肠最低。5-羟色胺免疫活性细胞位于胃的胃腺上皮、食道及肠的粘膜上皮,有圆形、椭圆形、梭形、楔形等,有的有胞突。文中讨论了5-羟色胺免疫活性细胞分布型的原因及形态与功能的关系。Using immunohistochemical technique, 5-Hydroxytryptamine immunoreactive (5-HT-IR) cells were observed in the alimentary tract of the toad Bufo melanostictus and the frog Rana nigromaculata . 5-HT-IR cells were distributed throughout the alimentary tracts of both species and located in the gastric glands of the stomach regions and in the intestinal or esophageal epithelium with variable frequencies. The density of the cell in B. melanostictus is the highest in the ileum, moderate in the jejunum and stomachus pyloricus, and the lowest in the duodenum and rectum. In R. nigromaculata the density of 5-HT-IR cells is the highest in the stomachus pyloricus, moderate in the ileum and jejunum, and the lowest in the esophagus, stomachus cardiacus and rectum. 5-HT-IR cells were spherical, elliptoid, coniform or spindle-shaped with long cytoplasmic process reaching to the lumen. The distribution mode of 5-HT-IR cells and the relationship between morphology and function are also discussed.福建省自然科学基金(No.D0320001

Fingerprint analysis on methyl fatty acid and its applications in microbial study

脂肪酸甲酯谱图分析方法(Fatty Acid Methyl Ester,FAME)是鉴于脂肪酸可作为生物标记物而发展起来的分析技术。本文介绍了FAME谱图分析方法及其在微生物学领域的应用,包括在微生物检测、鉴定和微生物多样性研究中的应用。Fatty acid is an important biomarker.Its application in biology research has become increasingly popular.The method of FAME fingerprint analysis is based on fatty acid's utilization as a biomarker.This paper describes the FAME fingerprint analysis and its applications for microbial studies,including identification of unknown microbes and assessment of microbial diversity.国家863计划项目(2006AA10A211);; 福建省发展和改革委员会重点项目[闽农产(2006)10号];; 福建省青年科技人才创新项目(2003.J046

3D-printed integrative probeheads for magnetic resonance

射频探头前端作为核磁共振设备的核心部件之一，极大程度的决定着系统实验性能的优劣。探头前端通常由射频线圈、射频电路及样品检测管道等部分组成。现有的射频线圈制作技术主要是通过手工或机械手段按照所需的线圈形状进行绕制。但是，当线圈结构较为复杂、不规则，或体积尺寸较小时，常规绕制方法便难以满足结构设计和制造的精度需求，因此造成线圈性能的劣化，增大检测区域的射频场不均匀性，对核磁共振检测产生负面影响。本研究中，利用3D打印熔融沉积制造或光敏树脂选择性固化技术精确加工出一体化磁共振探头前端，使用常温液态金属填充线圈模型管路形成射频线圈，搭建出稳定的一体化磁共振射频探头。利用高精度3D打印和液态金属灌注技术制备出包含有射频线圈和定制化样品管道结构在内的一体化磁共振射频探头前端，克服了传统磁共振三维微型线圈成型困难、与样品腔匹配程度差等问题，提高了探头的信噪比，为定制化的磁共振检测提供了新思路。 该工作由厦门大学电子科学与技术学院陈忠教授、游学秋副研究员和孙惠军高级工程师共同指导完成，博士研究生谢君尧为论文第一作者。厦门大学电子科学与技术学院黄玉清高级工程师、王忻昌副教授、倪祖荣助理教授、硕士研究生张德超，化学化工学院杨朝勇教授、博士研究生李星锐，萨本栋微米纳米科学技术研究院陈宏教授为合作作者。【Abstract】Magnetic resonance (MR) technology has been widely employed in scientific research, clinical diagnosis and geological survey. However, the fabrication of MR radio frequency probeheads still face difficulties in integration, customization and miniaturization. Here, we utilized 3D printing and liquid metal filling techniques to fabricate integrative radio frequency probeheads for MR experiments. The 3D-printed probehead with micrometer precision generally consists of liquid metal coils, customized sample chambers and radio frequency circuit interfaces. We screened different 3D printing materials and optimized the liquid metals by incorporating metal microparticles. The 3D-printed probeheads are capable of performing both routine and nonconventional MR experiments, including in situ electrochemical analysis, in situ reaction monitoring with continues-flow paramagnetic particles and ions separation, and small-sample MR imaging. Due to the flexibility and accuracy of 3D printing techniques, we can accurately obtain complicated coil geometries at the micrometer scale, shortening the fabrication timescale and extending the application scenarios.The work is supported by the National Natural Science Foundation of China (Grants U1632274, 11761141010, U1805261, 11475142, 22073078, and 61801411), and China Postdoctoral Science Foundation (2017M622075).研究工作得到国家自然科学基金、中国博士后科学基金等项目支持

Review of morphological and molecular identification of Pseudo-nitzschia, a bloom forming diatom

国家重点基础研究发展规划项目(2001CB409701);; 国家自然科学基金(3027118);; 中国科学院海洋研究所海洋生态与环境科学重点实验室开放基金课题

Catering Culture and Ethnic Boundary——Conversation about Catering Anthropology

以饮食与文化、食物与边界等方面,探讨了人类、饮食、文化的关联性,分析了饮食文化的层次性、多样性和丰富性。以饮食与文化、食物与边界等方面,探讨了人类、饮食、文化的关联性,分析了饮食文化的层次性、多样性和丰富性。Proceeding from the relationship between catering and culture, food and boundary, this conversation not Only covers the relevance among human beings, food and culture, the diversity and richness of Catering culture

饮食文化与族群边界——关于饮食人类学的对话

以饮食与文化、食物与边界等方面,探讨了人类、饮食、文化的关联性,分析了饮食文化的层次性、多样性和丰富性。以饮食与文化、食物与边界等方面,探讨了人类、饮食、文化的关联性,分析了饮食文化的层次性、多样性和丰富性