Expression and activity characterization of thrombosis targeting protein hu3D3V_H-tTF to blood vessel of lung cancer

目的制备一种用于肺癌血管靶向栓塞治疗的融合蛋白hu3D3VH-tTF,并鉴定其生物学活性。方法利用重叠PCR技术构建tTF与hu3D3VH的融合基因,克隆至表达载体pET22 b(+),在E.coliBL21(DE3)中表达,镍亲和色谱柱纯化目的蛋白。ELISA检测融合蛋白hu3D3VH组分与肺腺癌细胞A549选择性结合活性,凝血实验和FⅩ活化实验鉴定融合蛋白tTF组分的促凝血活性。结果获得序列正确的hu3D3VH/tTF/pET22 b(+)重组子,融合蛋白在E.coliBL21(DE3)中高效表达。纯化后的融合蛋白与肺腺癌细胞A549具有选择性结合活性,并能活化FⅩ、有效促发血液凝固。结论成功构建hu3D3VH/tTF/pET22 b(+)重组子,hu3D3VH/tTF融合蛋白具有hu3D3VH的选择性结合能力同时具有TF的促凝血活性,为开展选择性肺肿瘤血管血栓性栓塞研究奠定了基础。 【英文摘要】 Purpose To prepare the fusion protein of hu3D3VH-tTF for thrombosis targeting therapy of lung cancer and to analyze its biological activities.Methods Fusion gene hu3D3VH-tTF was constructed by overlap PCR,cloned into expression vector pET22 b(+),and expressed in E.coli BL21(DE3).The fusion protein hu3D3VH-tTF was purified through Nickel-affinity chromatography column.The selective binding activity of the hu3D3VH moiety of fusion protein was analyzed using ELISA and the coagulation activity of the tTF moiety...福建省自然科学基金项目(C0410004);; 厦门大学科技创新项目(XDKJCX20053026

Studies of preparation and properties of RGD-tTF water-based ferrofluids

目的探讨RGD-tTF水基磁流体通过磁场和RGD多肽在体外对内皮细胞双靶向的功能。方法通过化学沉淀法以柠檬酸钠为表面活性剂制备水基磁流体(MnFe2O4),弱酸改性后与重组的RGD-tTF融合蛋白结合,利用H-600透射电镜观测纳米粒径,以SUQID鉴定磁性,用MTT法、因子X活化检测和流式细胞仪检测RGD-tTF磁流体生物活性。结果成功制备出的水基磁流体能在磷酸盐缓冲液中稳定分布且具有生物兼容性,实验表明RGD-tTF与水基磁流体结合后对RGD和tTF生物活性均无显著影响,并证实在磁场的作用下能实现了水基磁流体对RGD-tTF的定位作用。结论一种具有内皮细胞双靶向功能的RGD-tTF水基磁流体已制备成功。 【英文摘要】 Purpose To study the property of RGD-tTF water-based ferrofluids double targeting EC304 cells in vitro by magnet and RGD peptide.Methods Water-based ferrofluids(MnFe2O4) were prepared by chemical precipitation method using citrate as surfactant, dispersed in weak acid to create surface charges,and coated with recombinant RGD-tTF protein.Proteins coated ferrofluids were characterized by H-600 transmission electron microscopy(TEM)and Superconducting Quantum Interference Device(SQUID),and its biological activi...教育部和厦门大学出国留学人员启动基金资

Analysis of Epistatic and QE Interaction Effects of QTL Controlling Heading Date in Rice (Oryza sativa L.)

抽穗期是水稻的重要农艺性状,深入了解其遗传效应对水稻育种实践具有重要现实意义。本研究利用基于明恢86×佳辐占、广陆矮×佳辐占两个重组自交系构建的SSR遗传图谱,应用混合线性模型方法对2003年晚季和2005年早季获得的两季水稻抽穗期数据进行QTL定位,并作加性效应、加性×加性上位互作效应及环境互作效应分析。两个群体共检测到10个控制抽穗期的QTL,分别位于1、2、3、6、7和10号染色体上,仅qHD10(广佳重组自交系中为qHD10-1)在两个群体中同时检测到,另检测到11对具有上位效应的互作位点,其中有5个是加性效应显著的QTL。环境互作检测中,发现明佳重组自交系的qHD10和广佳重组自交系的qHD7与环境存在显著互作,贡献率分别为0.34%和2.32%。本研究表明:两群体的抽穗期性状的遗传受环境因素影响较小,特别是明佳组合,较适合作为分子辅助育种的研究材料。Heading date (HD) is an important agronomic trait in rice, and detailed understanding of HD's genetic effects is significant for rice breeding practice. Quantitative Trait Locus (QTL) controlling HD of rice in different seasons and environments was analyzed using two Recombination Inbred Lines (RIL) respectively derived from crosses of Minghui86 × Jiafuzhan (MJ) and Guangluai × Jiafuzhan (GJ) by mixed linear model approach. 10 QTL with additive effects for HD were mapped in the chromosome 1, 2, 3, 6, 7 and 10 totally, and the qHD10 (qHD10-1 as in GJ-RIL) was mapped in both RIL. 22 QTL with epistatic effects for HD in these two RIL were detected, and 5 of them were QTL with additive effects. QTL× environment interaction was detected on qHD10 in MJ and qHD7 in GJ, each explain the phenotypic variation of HD with 0.34% and 2.32%. This demonstrates that QTL× environment interaction has minor influence to HD in these two RIL, and they are suitable for Molecular marker-assisted breeding, specially to MJ population

Transition Metal Promoted ZSM-5 Catalysts for the Conversion of Dimethyl Sulfide into Methanethiol

负载过渡金属的zSM-5催化剂用于催化甲硫醚(dMS)转化成甲硫醇(MT)的反应.实验结果表明,催化剂的甲硫醚转化率提高和甲硫醇选择性降低的趋势一样,都是以下顺序:CO/zSM-5>MO/zSM-5>nI/zSM-5>W/zSM-5.表征结果表明,由于过渡金属阳离子(W6+、nI2+、CO3+、MO6+)比Al3+活泼,而改性过程中W6+、nI2+、CO3+、MO6+分别代替了部分Al3+,使得改性催化剂对dMS和MT的化学吸附作用更强.过渡金属的引入使得zSM-5总酸度增强,提高了C―S键的裂解能力,从而改进了催化转化dMS的能力.研究结果发现,在转化dMS的过程中,金属活性位和酸性位之间通过强的协同效应起作用.ZSM-5-supported transition metal catalysts were prepared and used to catalyze the conversion of dimethyl sulfide(DMS) into methanethiol(MT).Test results indicated that the activities of the catalysts for the conversion of DMS increased as follows: Co/ZSM-5>Mo/ZSM-5>Ni/ZSM-5>W/ZSM-5.The decrease in MT selectivity followed the same trend.The characterization results showed that transition metal cations(W6 +, Ni2 +, Co3 +, Mo6 +) replaced some Al3 +sites leading to more active in chemiadsorption of DMS and MT since transition metal cations are more active than Al3 +.The incorporation of transition metals into ZSM-5enhances the total acidity of ZSM-5 and increases its capacity to rupture C―S bonds.This subsequently improves its catalytic behavior in the conversion of DMS.We found that the metal active sites and closely situated acidic sites have a strong synergistic effect when converting DMS

具通話功能的RFID Tag應用於照護之家

[[abstract]]跌倒是老年人容易發生的致命傷，因此在人口結構漸趨高齡化的社會，如何提高老年人在跌倒這方面的安全照護與品質是值得關注的議題；本研究針對容易跌倒的老年人，提出以無線射頻辨識系統（RFID）為基礎的語音跌倒通報系統，結合網路無遠弗屆的特性，應用在安養機構等單位上，作全天候不間斷的感測；即是在受照顧者身上配戴RFID Tag，在發生跌倒或緊急意外時，按下Tag上的呼叫鈕，通知並且與遠端的照護人員通話，達到即時通報、即時處理，以降低意外發生時伴隨的風險，也可以減輕照護人員在管理上的困難度，達到安全且可靠的照護體系

Hydrothermal Synthesis of 2D Ordered Macroporous ZnO Films

在由溶胶-凝胶法制备的纳米ZnO薄膜衬底上,以Zn(NO_3)_2·6H_2O和六亚甲基四胺(HMT)等摩尔浓度配制成前驱体溶液,在单层聚苯乙烯(PS)微球模板辅助下,采用水热法制备了具有规则多孔结构的ZnO薄膜.探讨了PS微球作为模板对ZnO纳米棒生长的限制作用以及柠檬酸钠在水热制备方法中对晶体生长的影响.利用扫描电子显微镜(SEM)和X射线衍射(XRD)表征了水热反应后所得二维有序ZnO膜表面形貌和取向性,测量了ZnO薄膜的光致发光(PL)光谱并研究其相应机理

耐磨防护涂层、其制备方法及应用

本发明公开了一种耐磨防护涂层、其制备方法及应用。所述耐磨防护涂层包括：覆盖于基底表面的硬质V过渡层、以及覆盖于所述硬质V过渡层上的VCN涂层。所述基底优选自不锈钢质机械运动基础件。所述制备方法包括：采用电弧离子镀技术，在基底表面沉积硬质V过渡层；采用电弧离子镀技术，在硬质V过渡层上继续沉积VCN涂层。本发明中VCN涂层主要是由VC及VN相组成，表现为一种典型的非晶镶嵌纳米晶结构，在大气和海水环境均表现出较低的摩擦系数及磨损率；硬质V过渡层能够改善不锈钢质机械运动基础件等基底与VCN涂层两者成分迥异材料间的结合性能，同时释放VCN涂层中的部分应力，从而有效的提高了VCN涂层与不锈钢质机械运动基础件的综合性能及使用寿命

可扩展和重构的二维自由空间光学互连系统

一种可扩展和重构的二维自由空间光学互连系统，其特征在于，包括：一阵列光源，其可接收数据输入；一二维光开关阵列；一前光学系统，该前光学系统位于阵列光源与二维光开关阵列之间；一阵列探测器，用于数据输出；一后光学系统，该后光学系统位于二维光开关阵列与阵列探测器之间；上述阵列光源、前光学系统、二维光开关阵列、后光学系统和阵列探测器位于同一光路上

耐高温防护涂层及其制备方法与应用

本发明公开了一种耐高温防护涂层及其制备方法与应用。所述耐高温防护涂层包括TiN过渡层以及形成于TiN过渡层上的AlTiN涂层；并且在所述AlTiN涂层中，0.67<Al原子数量-(Ti原子数量+Al原子数量)<0.9。所述制备方法包括：靶材选用Ti靶，工作气氛为N2，采用电弧离子镀技术在基体表面沉积TiN过渡层；同时以Al靶和Al67Ti33靶为靶材，采用电弧离子镀技术在TiN过渡层上继续沉积AlTiN涂层。本发明中AlTiN涂层铝含量高且Al-Ti比例可调，抗氧化温度可达到900℃，具有优良的耐高温氧化性能及防腐蚀性能，从而有效提高了AlTiN涂层与高速切削刀具及钛合金零部件的综合性能及使用寿命