Study on the analgesic effect and mechanism of intrathecal administration sinomenine on neuropathic pain rats

目的：本实验采用大鼠部分脊神经结扎神经病理性疼痛模型，通过鞘内置管中枢给药的方法，观察青藤碱对神经病理性疼痛大鼠热刺激、机械刺激等疼痛行为学变化，探讨和比较青藤碱的镇痛作用。并且通过分子生物学等方法，研究青藤碱镇痛作用的相关机制。 方法：通过大鼠颈部枕骨大孔进行鞘内置管，将一根长约8cm的PE-10导管置于大鼠腰骶膨大处。置管后大鼠适应5天后进行部分脊神经结扎手术。术后观察大鼠一般情况和体重变化。术后第7d单次鞘内注射不同浓度青藤碱，观察大鼠6h内急性疼痛行为学的变化，术后第8d至15d，连续鞘内鞘内注射不同浓度青藤碱，观察大鼠的慢性疼痛行为学变化，术后第15d给药后处死大鼠，取脊髓背角，运...Objective：On this study, we use the spinal nerve ligation model to simulate the neuropathic pain to observe the change of mechanical withdraw threshold and thermal withdraw legacy and compare the analgesic effect by intrathecal administration of sinomenine. We also explore the analgesic mechanism of sinomenine by molecular biochemical methods. Methods：A 8cm PE-10 catheter was placed in the lumbos...学位：医学硕士院系专业：医学院_药理学学号：2452014115359

カルニチン補充と栄養指導を行った自家中毒の男児例

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肝の嚢胞性腫瘍の一切除例

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