Effect of Yuhan Nuanwei Cream Acupoints Stiking Therapy on Gastric Mucosa TNF-α,PCNA of Mice with Chronic Atrophic Gastritis

目的:研究御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎(CAG)大鼠胃黏膜肿瘤坏死因子-α(TNF-α)、增殖细胞核抗原(PCNA)表达的影响,探讨御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎癌前病变CAG大鼠胃黏膜损伤修复的作用机制。方法:大鼠随机分为正常组、模型组、御寒暖胃膏贴敷胃经穴组、药物对照组,采用综合干预方法复制慢性萎缩性胃炎癌前病变大鼠模型,肉眼下观察大鼠胃黏膜损伤指数,光镜下观察胃黏膜组织的病理变化,采用酶联免疫分析法测定胃黏膜细胞中TNF-α、PCNA的表达水平。结果:与正常组比较,模型组的大鼠胃黏膜组织病理学检查提示存在腺体的萎缩和一定程度的细胞异型增生,大鼠胃黏膜细胞中TNF-α、PCNA的表达水平升高,差异有统计学意义(P<0.05);与模型组比较,御寒暖胃膏贴敷胃经穴组和药物对照组大鼠胃黏膜组织的病理损伤得到修复,大鼠胃黏膜细胞中TNF-α、PCNA的表达水平降低,差异有统计学意义(P<0.05);结论:御寒暖胃膏贴敷胃经穴可以促进CAG大鼠胃黏膜损伤的修复,该作用可能是通过调节胃黏膜细胞TNF-α、PCNA的表达水平来实验的。Objective:To study the effect of Yuhan Nuanwei cream(YNC)acupoints stiking therapy on gastric mucosa tumor necrosis factor-α(TNF-α) and proliferating cell nuclear antigen(PCNA) expression of mice with chronic atrophic gastritis(CAG),so to discuss the renovation mechanism of YNC on CAG precancerous lesions. Methods:The mice were divided into normal group,model group,YNC group,drug control group. Duplicate mice model with CAG precancerous lesions by the method of comprehensive intervention,observe gastric mucosal lesion index of mice with the naked eyes,observe pathological change of gastric mucosa tissue under light microscope,measure expression level of TNF-α and PCNA in gastric mucosa cells by the method of enzyme-linked immunoassay. Results: Comparing with normal group, mice gastric mucosa histopathological examinations in model group reminded that there exist gland atrophy and some degree of cell atypical hyperplasia,the expression level of TNF-α and PCNA in mice gastric mucosa cell are increased,differences being significant(P<0.05). Comparing with model group,pathological damage of mice gastric mucosa tissue in YNC group and drug control group obtained obvious repair,the expression level of TNF-α and PCNA in mice gastric mucosa cell were dropped, differences being significant(P<0.05).Conclusion:The therapy of YNC acupoints stiking therapy can promote the renovation of CAG mice gastric mucosal lesion. This fuction may be experimented by regulating the expression level of TNF-α and PCNA in mice gastric mucosa cell.深圳市科技研发资金项目(JCYJ20130401105615482

御寒暖胃膏穴位贴敷对胃癌前病变大鼠胃黏膜的影响

目的:研究御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎癌前病变大鼠胃黏膜的影响,探讨御寒暖胃膏贴敷胃经穴对慢性萎缩性胃炎癌前病变大鼠胃黏膜损伤修复的作用机制。方法:大鼠随机分为正常组、模型组、御寒暖胃膏贴敷胃经穴组、御寒暖胃膏贴敷对照点组、药物对照组,采用综合干预方法复制慢性萎缩性胃炎癌前病变大鼠模型,肉眼下观察大鼠胃黏膜损伤指数,光镜下观察胃黏膜组织的病理变化,彩色多普勒观察胃黏膜的血流量。结果:与模型组比较,御寒暖胃膏贴敷胃经穴组和药物对照组大鼠胃黏膜损伤指数值均显著降低(P0.05),胃黏膜组织的病理损伤未得到明显修复,胃黏膜血流量未见显著升高(P>0.05)。结论:御寒暖胃膏贴敷胃经穴可以促进慢性萎缩性胃炎癌前病变大鼠胃黏膜损伤的修复,增加胃黏膜的血流量,并且存在经脉-脏腑特异相关性。深圳市科技研发资金项目(JCYJ20130401105615482

不停跳心内直视手术对心肌细胞凋亡的影响

【目的】评价不停跳体外循环心内直视手术对心肌细胞凋亡的影响和探讨其临床意义。【方法】停跳心内直视手术患者25例（A组）,不停跳心内直视手术患者20例（B组）,取心肌组织作TUNEL细胞凋亡检测,比较分析术前和术后心肌细胞凋亡率并参照围手术期肌钙蛋白T（cTnT）检测。【结果】两组患者均无低心输出量综合征的发生。心肌细胞凋亡指数A组（0.003±0.002）和B组（0.004±0.003）术前无明显差别（P〉0.05）;术后心肌细胞凋亡指数A组（0.012±0.008,P〈0.001）和B组（0.006±0.004,P〈0.001）均高于术前;A、B两组术后心肌细胞凋亡指数差别有明显差别（P〈0.05）。围手术期cTnT水平A组在CPB末和术后2 h明显高于B组（P〈0.05）。【结论】本实验提示不停跳体外循环心内直视手术引起心肌细胞凋亡和心肌损害小于停跳手术

ST段抬高型急性心肌梗死院前溶栓治疗中国专家共识

急性心肌梗死仍然严重威胁我国人民健康,在我国广大城乡地区,形势更为严峻[1,2]。及时救治急性心肌梗死患者,降低死亡率和保护心脏功能刻不容缓。鉴于我国的实际情况,院前溶栓治疗在大城市以外的城乡地区具有重要意义。为此,中国医师协会胸痛专业委员会及中国医学救援协会心血管急救分会专门组织有关专家制订了本共识,旨在帮助院前医疗急救人员对急性心肌梗死患者选择最佳

Academic origin,development and characteristic of Xujiangacupuncture school

追溯旴江针灸流派的起源年代、代表性医家及医学著作,探寻旴江针灸流派形成的学术源流,并对旴江流派的学术特色进行归纳总结,以便更好地继承旴江针灸流派的学术精髓,促进旴江针灸流派的创新和发展。The origin time,representative physicians and medical works of Xujiangacupuncture school were traced,so as to explore the academic origin and development and summarize the academic characteristic of Xujiang acupuncture school,which could make a better inheritance of academic essence and prompt the innovation and development of Xujiangacupuncture school.江西中医药大学校级研究生创新专项资金项目:JZYC12B0

The Influence of Cold-Removing and Stomach-Warming Ointment on Gastric Precancerous Lesions in Rats

目的：观察御寒暖胃膏对胃癌前病变大鼠胃黏膜的影响。方法：将50只SD大鼠随机分为正常组、模型组、御寒暖胃膏贴敷胃经穴组、御寒暖胃膏贴敷对照点组和药物对照组,每组各10只。采用综合干预方法复制慢性萎缩性胃炎癌前病变大鼠模型,正常组喂饲标准饲料,不予任何处理。模型组自第21周开始,每天捆绑1 h,并予胶布贴敷腹部及双下肢,每只每天按0.001 m L·g~（-1）的剂量经口灌服纯净水。御寒暖胃膏贴敷胃经穴组、对照点组自第21周开始,每天将各组大鼠捆缚于鼠板,将膏药涂于所选部位上,每日1 h。每只每天按0.001 m L·g-1的剂量经口灌服纯净水。药物对照组给予维酶素溶液,按21.6 mg·kg~（-1）的药量给药,每日1次,每天捆绑1 h,并予胶布贴敷腹部皮肤及双下肢。肉眼下观察大鼠胃黏膜损伤指数,光镜下观察胃黏膜组织的病理变化,彩色多普勒观察胃黏膜的血流量。结果：与模型组比较,御寒暖胃膏贴敷胃经穴组和药物对照组大鼠胃黏膜损伤指数值均显著降低,差异具有统计学意义（P〈0.005）;胃黏膜组织的病理损伤得到明显的修复、胃黏膜血流量均显著升高,差异具有统计学意义（P〈0.005）。御寒暖胃膏贴敷对照点组大鼠胃黏膜损伤指数值未见明显降低,差异无统计学意义（P〉0.005）;胃黏膜组织的病理损伤未得到明显修复、胃黏膜血流量未见显著升高,差异无统计学意义（P〉0.005）。结论：御寒暖胃膏贴敷胃经穴可以促进慢性萎缩性胃炎癌前病变大鼠胃黏膜损伤的修复,增加胃黏膜的血流量,并且存在经脉—脏腑特异相关性。Objective： To observe the influence of Cold-Removing and Stomach-Warming Ointment on gastric precancerous lesions in rats. Methods： 50 SD rats were randomly divided into the normal group,the model group,Cold-Removing and Stomach-Warming Ointment stomach meridian points group,Cold-Removing and Stomach-Warming Ointment control point group,drug control group,with 10 rats in each group. Gastric precancerous lesions in rats models were copied by using comprehensive intervention method.The ones in the normal group were fed with standard diet without any treatment. Since the twenty-first week,the ones in the model group were tied up for one hour,with belly and lower limbs being applied,and took pure water orally with the amount of 0. 001 m L·g~（-1） each day. Since the twenty-first week,the ones in Cold-Removing and Stomach-Warming Ointment stomach meridian points group,Cold-Removing and Stomach-Warming Ointment control point group were bounded to the rats board and applied ointment on the selected points,one hour each day. Each rat in the two groups took pure water orally with the amount of 0. 001 m L·g~（-1） each day. The ones in drug control group were treated with Dimensional enzyme solution with the amount of 21. 6mg·kg~（-1）each day. They were tied up for one hour each day,their belly and lower limbs were applied ointment with adhesive tape. Then the gastric mucosal injury index was observed by naked eye,and the pathological changes of gastric mucosa were observed under light microscope,and the blood flow of gastric mucosa was observed by color Doppler. Results： Compared with the model group,indexes of gastric mucosal injury in Cold-Removing and Stomach-Warming Ointment stomach meridian points group,and drug control group were significantly reduced,the difference was statistically significant（ P〈0. 005）. Their pathological damage of gastric mucosa was significantly repaired and the gastric mucosal blood flow was significantly increased,and the differences were statistically significant（ P深圳市科技研发资金（编号：JCYJ20130401105615482

Comparison study of infantile tuina between LI Dexiu and LIU Kaiyun

以《李德修小儿推拿秘笈》和刘开运《小儿推拿疗法》为蓝本,研究两者在操作手法、穴位考订以及治疗原则方面的异同点,为李德修与刘开运小儿推拿的更好推广应用提供理论依据。With esotericaof Infantile Tuina by LI Dexiu and Infantile Tuina Therapy of LIU Kaiyun,the differences and similarities of manipulations,acupoints and the principles of treatment were studied so as to provide theoretical evidence to popularize tuinaof LI Dexiu and LIU Kaiyun