Analysis of partial sequences of Hepatitis E virus isolated from human and swine in a certain area of Jiangsu province

目的:调查了解江苏省某农村地区人源与猪源戊型肝炎病毒(HEV)的相关性。方法:应用逆转录-巢式聚合酶链法(rT-PCr)对同一地区内一般人群中HEV IgM阳性者、急性戊型肝炎患者血标本和生猪胆囊标本进行HEV rnA检测,并对HEV rnA阳性标本进行克隆测序和序列分析。结果:一般人群中检出的HEV基因1、4型比例相近,临床散发性戊肝病例绝大部分为HEV-4型病毒感染所致(95.24%);生猪胆汁标本PCr阳性率为12.11%,猪源HEV基因分型均为HEV-4型;分离于人和生猪的HEV-4型病毒高度同源,同源性81%~97%。结论:该地区人群HEV基因分型以HEV-4型为优势毒株;猪源HEV均为基因4型;人源和猪源的HEV-4型病毒高度同源,猪是当地HEV主要宿主动物之一。Objective:To explore the correlation between swine and human hepatitis E virus(HEV) in Jiangsu province.Methods:Specimens collected from HEV-IgM positive people of the general population,patients with acute hepatitis E and cholecyst from swine in the same area,were detected for HEV RNA by nested reverse transcription polymerase chain reaction(RT-PCR).HEV-positive samples were further analyzed by direct nucleotide sequencing.Results:Among the HEV IgM carriers,8 positive cases were identified,and the subtype of HEV were type 1 and type 4.The majority cases of clinical sporadic HEV were type 4(95.24%).The HEV-positive rate of swine bile samples was 12.11%,and the virus genotype was type 4.The type 4 HEV of human and swine showed a high degree of homology(81%～97%).Conclusion:Both HEV-1 and HEV-4 were found in human in this area with the dominant type of HEV-4.All the swine HEV-positive samples showed HEV-4 genotype.The HEV-4 of human and swine in the Dongtai area showed a high degree of homology.These findings suggest that swine may be one of the main hosts of HEV in this region

Analysis on the epidemiological characteristics of the sporadic cases with hepatitis E infection in the rural area in Jiangsu province

目的了解江苏省农村散发性戊型肝炎的流行病学特征。方法通过建立覆盖市、镇、村三级医疗卫生服务机构的疑似肝炎主动监测网络,系统全面地监测戊型肝炎病例的发病情况。结果主动监测网络的敏感性明显高于网络报告系统,能更加准确、全面地掌握戊肝的发病规律。结果显示戊型肝炎病例占疑似急性肝炎病例的26.7%,男性戊肝发病率高于女性(P<0.01);发病随着年龄增长而上升,多见35岁以上人群;全年均有发病,冬春季节较高;戊型肝炎病毒株HEV1、4型并存,但以HEV4型为主(92.5%)。结论疑似肝炎主动监测系统数据显示目前戊肝发病率有被低估的风险。To understand the epidemiological characteristics of the sporadic cases with hepatitis E infection in the rural area of Jiangsu province,the prevalence of hepatitis E infection was surveyed systemically and completely through the initiatively monitoring system for the suspected cases of hepatitis E covering the city,town and village levels of medical and health service organizations.It was found that the initiatively monitoring system for suspected hepatitis E cases reflected the incidence of hepatitis E more accurately and comprehensively and was proved to be more sensitive than the network reporting system.The cases with hepatitis E accounted to 26.7% of the suspected cases with acute hepatitis and they were more frequent in male than in female.The incidence of hepatitis E increased with advance of age and most of cases occurred with age over 35.Estimated incidence was stable on the whole year,but rose up in winter and spring.The HEV type 1 and type 4 co-existed,but the HEV type 4 was proved to be the main type(92.5%).From the above observations,it seems that the initiatively monitoring system for the suspected cases of hepatitis E may be under-estimated for the risk of the hepatitis E infection in rural area of Jiangsu province.国家高技术研究发展计划(863计划)(2006AA02A209)资

散发型戊型肝炎与急性乙肝临床特征比较

目的比较散发型急性戊型肝炎与乙肝临床特征。方法分析戊肝和急性乙肝间年龄、性别、肝脏损伤、症状体征以及病程的差异。结果急性肝炎病例中,戊型肝炎最常见(28.0%),急性乙肝次之(9.2%);戊肝平均发病年龄为56.3±13.1岁,急性乙肝为43.0±12.5岁,平均发病年龄戊肝较急性乙肝大(t=4.4723,P<0.0001),均为男性多发;两者常见的临床症状和体征基本相当,但戊肝患者黄疸症状更多见(P<0.05);戊肝病程较急性乙肝长(t=3.7249,P=0.0003);通过年龄性别进行1:1匹配分析,戊肝比急性乙肝对肝脏的损伤程度严重(t=3.5978,P=0.0019)。结论戊肝多见于中老年,急性乙肝多见于中青年,均为男性多于女性。临床特征比较戊肝较急性乙肝更为严重

KH型喷嘴的研制及技术发展

应用国外液滴雾化研究成果,结合流体力学基本原理,分析了KH喷嘴的雾化机理,给出了KH喷嘴的物理模型、理论计算和实验结果,确定了喷嘴结构型式和尺寸。证实了喷嘴的压力降是控制雾化粒度的关键。分析了油品、蒸汽量、喷嘴结构、操作参数等对雾化粒度的影响。提出喷嘴质量的评定标准。总结了使用KH喷嘴厂家的蒸汽量、干气、液体收率以及能耗情况

Disease burden of hepatitis E in rural areas in east China

目的分析戊型肝炎的疾病负担,为卫生决策提供科学依据。方法采用问卷调查方式,对江苏省东台市农村人群戊型肝炎主动监测网络收集的病例进行调查,获得戊型肝炎的疾病负担情况。结果戊型肝炎的总经济负担为2 910 362.60元,人均经济负担为11 641.45元,是东台市农村居民人均纯收入的1.26倍;失能调整生命年为28.94人年,患者的负担强度为115.78人年/千人。结论戊型肝炎的疾病负担较重,应加大防制力度。In order to analyze the disease burden of hepatitis E and provide a scientific basis for health decision-making,the cases collected by the active hepatitis E monitoring network on rural population in Dongtai of Jiangsu Province were investigated by questionnaires to access the disease burden of hepatitis E cases.It was found that the total economic burden of hepatitis E was 2 910 362.60 yuan and per capita was 11 641.45 yuan,which was 1.26 times the per capita net income of rural residents in Dongtai;the burden of hepatitis E was 28.94 disability adjusted life years(DALYs) and the cases burden of strength was 115.78 DALYs/ thousand.From the above observations,it seems that the burden of hepatitis E was heavy,thus the prevention efforts should be increased.国家高技术研究发展计划(863计划)(2006AA02A209)资