3 research outputs found

    BIOACCUMULATION OF CADMIUM ION

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    [中文文摘]研究了表达外源特异性Cd2+转运系统的基因工程菌E.coliTOP10F’对Cd2+的富集行为。重组菌获得了比宿主菌高6倍的Cd2+富集能力,而且富集行为受pH变化的影响也大为降低。Na+、Mg2+以及Cu2+、Hg2+、Pb2+和Ni2+等共存离子的存在都对重组菌的Cd2+富集产生抑制,其中Cu2+的抑制作用最强,Ni2+最弱。EDTA的存在严重影响重组菌对Cd2+的生物富集,但柠檬酸的影响却很小。[英文文摘]Escherichia coli TOP10F’ was genetically engineered to express a Cd2+ transport system for the evaluation of its Cd2+ bioaccumulation from aqueous solution. A six-folds increase of Cd2+ uptake was obtained by the recombinant bacteria compared with the original host strain. Within the test of pH range (4~9), the recombinant cells showed high resistance to pH variation, retaining up to 9mg/g of Cd2+ bioaccumulation. The presence of Na+, Mg2+, Cu2+, Pb2+, Hg2+ and Ni2+ inhibited Cd2+ uptake to different extents, respectively, with the effect of Cu2+ being most significant and Ni2+ being slightest. The existence of 2mmol/L Citric acid didn't influence Cd2+ bioaccumulation remarkably, while 0.1mmol/L EDTA resulted in more than 90% loss of Cd2+ binding capacity, suggesting EDTA could exert a severe adverse effect on Cd2+ uptake by recombinant strain.浙江省自然科学基金(M203130); 2003年教育部优秀青年教师资助计划

    大连极紫外相干光源

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    先进光源的发展在前沿科学研究中发挥的作用越来越重要。近十年来,飞速发展的自由电子激光技术为科学家们提供了探索未知世界、发现新科学规律和实现技术变革的重要工具。建成的大连极紫外(EUV)相干光源的运行波段为50~150nm,单脉冲能量大于100μJ,且可提供10-12 s和10-13 s量级的超快激光脉冲,是我国第一台自由电子激光用户装置,并且是国际上唯一运行在极紫外波段的自由电子激光用户装置,在世界范围内为用户提供具有高峰值亮度和超短脉冲的极紫外激光。大连EUV相干光源是由国家自然科学基金委资助、由中国科学院大连化学物理研究所和上海应用物理研究所共同承担的重大科学仪器研制项目,目标是打造一个以先进极紫外光源为核心、主要用于能源基础科学研究的光子科学平台
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