The Maritime Trade of Zhangzhou in Global Context from the 15th to Early 17th Centuries

本文论述了15-17世纪初资本主义兴起扩张、西方殖民者东来之时漳州私人海外贸易的兴起发展以及与西方沟通交往的过程，力图把漳州这一时期的海外贸易置于全球视野中，来探寻其作用与影响 第一章绪论，阐述了选题缘由、思路；本文的特点与贡献等。 第二章回顾并评价了国内外研究现状，并就此提出了自己的看法。 第三章分为两节，分别论述了15-17世纪初国内外的形势背景与世界的变化发展。 第四章共分三节。第一节介绍了漳州的地理位置与漳州人的性格特长，是为漳州海外贸易发展的前提之一。第二节全面叙述了15-17世纪初漳州私人海外贸易的兴起和发展，时逢海道大通、西方殖民者东来，所以这一时期漳州的海外贸易构成了世...This article outlines the emergence of individual overseas trade of Zhangzhou when the western colonist came eastward under the capitalism expansion in 15~17 century. It seeks to inquire the influence of Zhangzhou overseas trade in the global context. The introduction in the first chapter illustrates the purpose, outline, characteristics and contribution of the theme of this paper. The second ch...学位：历史学硕士院系专业：人文学院历史学系_世界史学号：X20040200

The Molecular Mechanism of the Regulation of AMPK Phosphorylation and Activity by Hypo2

腺苷酸活化蛋白激酶(AMP－activatedproteinkinase，AMPK)是细胞重要的“能量感受器”，在调节代谢和能量平衡等方面具有重要的作用。近年来研究显示，AMPK不仅在调节糖脂代谢、血压及免疫功能等方面扮演重要角色，同时还参与了多种代谢性疾病的发生和发展，因此AMPK相关作用机制的研究可能为肥胖、糖尿病（DM）、代谢综合征（MS）和肿瘤的治疗提供新的途径。我们实验室检测到了一个崭新的与AMPK相互作用的蛋白命名为Hypo2，它们的结合随着葡萄糖饥饿时间的延长而增强。我们发现随着Hypo2的表达量上升AMPKα1的第172位苏氨酸的磷酸化水平也随之上升，从而上调了AMPK的活性。...As a metabolic sensor, the serine/threonine protein kinase AMP -activated protein kinase(AMPK) promotes the adaptation of cells to signals arising from nutrients, hormones, and growth factors. It regulates metabolism and energy homeostasis. Recent studies show that AMPK is not only involved in the regulation of glucose and lipid metabolism , blood pressure and immunity, but also in the development...学位：理学硕士院系专业：生命科学学院生物学系_生物化学与分子生物学学号：2172009115207

A Study on Determinants of Accounting Transparency:Evidence from China's Listed Companies

如何提高上市公司会计透明度，多年来一直是理论界和实务界着力探讨的问题。笔者认为，解决该问题的前提是要理清影响会计透明度的多方面因素。本文结合中国制度背景对上市公司会计透明度的影响因素展开研究。在对比和借鉴相关研究的基础上，本文确定以会计盈余相关质量特征来构建会计透明度指数，并使该指数及其子指数便于从整体、短期和长期趋势等角度来衡量上市公司会计透明度；基于信息观、不完全契约理论和投资者保护观等理论视角，剖析影响会计透明度的深层次原因，并结合现有研究和中国制度背景，将影响会计透明度的因素划分为内部和外部因素；应用中国上市公司数据，分别检验了制度环境、外部治理机制、董事会有效性和股权结构特征对会计透...Improving accounting transparency of listed companies has been discussed over decades both by academia and practitioners. Sorting out the determinants of accounting transparency will be helpful to solve the problem. The author studies the determinants of accounting transparency on the background of China's institution in this paper. Firstly the author constructs three accounting transparency indic...学位：管理学博士院系专业：管理学院会计系_会计学学号：B20041102

我国会计师事务所的竞争弱势及应对措施

本文就我国注册会计师行业的竞争弱势进行剖析,并有针对性地提出应对措施,以期对提升我国注册会计师行业的竞争力有所裨益

醇溶型无机锌车间底漆的电化学行为研究

使用电化学方法对2种不同的醇溶型无机锌车间底漆进行了研究,结合浸泡以及盐雾的实验结果,发现2种车间底漆中锌含量对阴极保护时效有影响,但不是决定车间底漆防锈能力的唯一因素,锌含量与防锈颜料对于车间底漆的防锈性能有协同效果。其中,锌粉在早期提供阴极保护的作用,而防锈颜料在后期对基底提供防锈保护。对于锌粉含量相对较高的车间底漆,锌粉并不是从一开始就全部参加阴极保护作用,而是一部分具有激活潜力的锌粉在另一部分锌粉率先反应之后才由\"未激活状态\"转变为\"激活状态\"进而提供阴极保护的作用

Preliminary Study on Regulation of XIOsPR10 Gene Expression in Rice

为探索水稻Pr10蛋白在水稻抗细菌性条斑病中的作用,构建了XIOSPr10基因的植物过量表达载体P1301-XIOSPr10及rnAI表达载体PdS1301-XIOSPr10,通过农杆菌介导分别转化水稻愈伤组织,获得了相应的再生植株。经guS检测和PCr分析,证实XIOSPr10基因以及rnAI片段分别整合到水稻再生植株基因组中;半定量rT-PCr分析显示,过量表达植株中XIOSPr10基因的表达量高于对照,而rnAI转基因植株中XIO-SPr10基因的表达被抑制。In order to study the function of rice XIOsPR10 gene which is related to the plant disease resistant reaction,we constructed plant over expression and RNAi expression vector and integrated into the genome of rice via Agrobacterium tumefaciens EHA105,respectively.The transgenic cultivars were identified by PCR and GUS gene expression detection.The analysis result showed that the transcription level of XIOsPR10 of over-expression transgenic rice was higher than that of control.In construct,XIOsPR10 gene expression was blocked in RNAi transgenic rice.科技部863专题(2007AA10Z132);国家重大科技专项(2008ZX08001-001;2009ZX08009-045B);教育部重点项目(01102

Expression of pin1 gene in lung cancer and its significance

背景与目的:Pin是人类高度保守的特异性磷酸化肽基脯氨酰顺及异构酶,它作用于脯氨酸所形成的肽键,并且仅使磷酸化pSer/Thr-Pro发生异构化,这一磷酸化后调控机制能诱导磷酸蛋白的构像变化,使其发挥功能。近来的研究显示这种新的调控机制在许多生理过程中起重要的调节作用,一旦失调便导致一系列人类疾病。如癌症患者体内Pin1表达异常增高,并调控多种癌基因的信号通路。本研究探讨pin1基因在非小细胞肺癌(NSCLC)中的表达及其意义。方法:利用免疫组织化学方法、定量PCR方法分别检测肺癌组织和正常肺组织中Pin1基因在翻译水平和mRNA水平上的表达差异。结果:在蛋白质水平上,Pin1的表达在正常/癌两种不同的肺组织中,表达量差异有显著性;而在mRNA水平上,Pin1的表达量在正常/癌两种不同的肺组织中,差异无显著性。结论:Pin1在NSCLC组织中的表达,可能是在翻译水平受到了调控;我们的研究进一步证实:Pin1在NSCLC组织中存在蛋白水平的过量表达,这对利用Pin1作为NSCLC组织检测标志物提供了理论依据。Background and purpose:Pin1 is a highly conservative enzyme that isomerizes only the phosphorylated Ser/Thr-Pro bonds in certain proteins, thereby inducing conformational changes. Recent results indicate that such conformational changes following phosphorylation are a novel signaling mechanism pivotal in regulating many cellular functions. Overexpression of Pin1 is prevalently found in human cancers.We studied expression of pin1 in non-small cell lung cancer and its implication in clinical. Methods:We detected the differential expression level of Pin1 protein in lung cancer specimen and normal lung tissues by immunohistochemical staining. Real-time quantitative PCR was also applied to detect the mRNA differential expression level in lung cancer tissues and normal lung tissues. Results:Pin1 protein is overexpressed in lung cancer at the protein level. On the other hand, the expression level of Pin1 mRNA in lung cancer tissue has no significant change compared with normal tissue. Conclusion:It demonstrated that the Pin1 expression in lung cancer might be regulated by translation mechanism.Our data implicate that Pin1 may serve as a valuable molecular marker for human cancer

酪氨酸激酶抑制药GW2974诱导乳腺癌细胞BT474耐药的代谢机制研究

目的通过比较酪氨酸激酶抑制药GW2974给药后母本细胞株BT474与耐药株r BT474之间代谢相关因子mRNA表达的差异,探讨r BT474可能的耐药机制。方法收集对数生长期r BT474和BT474细胞,接种于96孔板,加入含有GW2974的培养基,分别于孵育0,12,24和48 h后,通过噻唑蓝法检测BT474与r BT474细胞的增殖情况。用逆转录实时定量-聚合酶链反应检测12种相关代谢因子葡萄糖转运体4(GLUT4)、2,6-二磷酸果糖激酶(PFK-2)、丙酮酸激酶2(PKM2)、乳酸脱氢酶(LDHA)、丙酮酸羧化酶(PC)、6-磷酸果糖激酶(G-6-PD)、脂肪酸合成酶(FASN)、脂酰肉碱转移酶1 (CPT1A)、葡萄糖调节蛋白75(GRP75)、电压依赖性阴离子通道蛋白1 (VDAC1)、葡萄糖调节蛋白78(GRP78)和钙网蛋白(CALR)的mRNA在BT474细胞和r BT474细胞中给药前后的表达水平。结果给予GW2974后,母本细胞BT474中糖脂代谢相关因子GLUT4、PFK-2、PKM2、LDHA、PC、G-6-PD、FASN和CPT1A的mRNA表达水平均明显下调,线粒体应激因子GRP75、VDAC1与内质网应激因子CALR表达水平均明显下调,内质网应激因子GRP78表达明显上调。而在耐药株r BT474中给药后这12种代谢相关因子的mRNA表达水平均明显上调。结论耐药株r BT474可能通过调控糖脂代谢以及细胞应激等过程使细胞耐受性增强。福建省自然科学基金面上项目(2012J01417

对比增强3D STIR SPACE序列对子宫内膜异位症患者骶丛神经异常的显示

目的探讨增强 3D STIR SPACE序列对盆腔子宫内膜异位症患者骶丛神经异常的显示价值，并与增强3D VIBE序列进行对比。方法病例组包括30名子宫内膜异位症患者，对照组包括20名健康女性，均接受盆腔MRI检查，包括对比增强3D STIR SPACE序列和增强3D VIBE序列。评估骶丛神经显示的图像质量以及子宫内膜异位症患者中的骶丛神经侵犯。结果对照组和病例组中，3D STIR SPACE序列和3D VIBE序列均能很好地显示骶丛神经纤维（P<0.001）。阅片者应用3D-STIR-SPACE序列对病变的诊断信心分数一致性较强（Kappa=0.684，P<0.001），应用3D-VIBE序列的诊断信心分数一致性一般（Kappa=0.130，P=0.386）。结论对比增强T2-SPACE序列和3D VIBE序列能够清晰显示骶丛神经，前者对盆腔子宫内膜异位症患者骶丛神经异常的显示更有优势

Study on the Relationship between Structure of Supramolecular Ion Material and Performance of Humidity Sensing

相对湿度是许多领域的关键参数，环境湿度与人们的生活密切相关，因此对湿度进行测量和控制是各个领域中值得关注的问题之一. 在前期的研究中，作者制备了一种新型的超分子离子材料（SIM），它是由基于咪唑的双阳离子（如1,10-双（3-甲基咪唑-1-基）癸烷，C10(mim)2）和电活性二阴离子（如2,2\'-连氮基-双（3-乙基苯并噻唑啉-6-磺酸），ABTS）组成的，发现其对湿度具有敏感且快速的响应. 在此基础上，本文制备了6种不同碳链（C4，C6，C8，C10，C12，C14）的咪唑基化合物，发现其中3种（C10，C12，C14）可与ABTS形成水稳定的SIM. 循环伏安法、计时电流法以及石英晶体微量天平表征了这些超分子离子材料的湿度传感性能，发现基于C12的SIM具有最佳的湿度传感性能. 同时，SEM结果显示随着碳链的增加，离子材料的厚度变薄并且形态变得不规则. 因此，作者认为疏水作用和材料比表面积均会影响湿度传感的灵敏度. 本研究为发展新的湿度响应的离子传感材料奠定了基础.Humidity measurement and control is one of the most notable issues in various areas, such as climate, industry, agriculture, electronics, especially human comfort and health. In our previous study, we have found that a new kind of supramolecular ionic material (SIM), consisting of an imidazolium-based dication (e.g., 1,10-bis(3-methylimidazolium-1-yl) decane, C10(mim)2) and electroactive dianionic (e.g., 2,2\'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), ABTS), shows ultrasensitive and ultrafast response towards humidity sensing. Herein we prepared six kinds of imidazolium-based dications with different carbon chain lengths (i.e., C4, C6, C8, C10, C12, C14), and found that three of them (i.e., C10, C12, C14) could form water-stable SIM with ABTS. Cyclic voltammetry, chronoamperometry and quartz crystal microbalance were used to characterize the humidity sensing performance of these supramolecular ionic materials. The results show that the C12-based SIM has the best humidity sensing performance compared with the other two kinds of SIM. Moreover, SEM images show that the thickness of the ionic material became thinner with the increase of the carbon chain length. We, thus, conclude that both the hydrophobic interaction and the specific surface area of SIMs would contribute to the performance of humidity response. This paper would lay the foundation for the development of new ionic compound for humidity sensing.This research was supported by the National Natural Science Foundation of China (No. 21475138).作者联系地址：1. 湘潭大学化学学院，环境友好化学与应用教育部重点实验室，湖南 湘潭 411105；2. 北京分子科学国家实验室，中国科学院化学研究所活体分析化学重点实验室，北京 100190Author's Address: 1. Key Laboratory of Environmental Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China; 2. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, the Chinese Academy of Sciences, Beijing 100190, China通讯作者E-mail：[email protected]