Dual immobilization and magnetic manipulation of magnetic nanoparticles

By suitably bio-functionalizing the surfaces, magnetic nanoparticles are able to bind specific biomolecules, and may serve as vectors for delivering bio-entities to target tissues. In this work, the synthesis of bio-functionalized magnetic nanoparticles with two kinds of bio-probes is developed. Here, the stem cell is selected as a to-be- delivered bio- entity and infarcted myocardium is the target issue. Thus, cluster designation-34 (CD-34) on stem cell and creatine kinase-MB (CK-MB) (or troponin I) on infarcted myocardium are the specific biomolecules to be bound with bio-functionalized magnetic nanoparticles. In addition to demonstrating the co-coating of two kinds of bio-probes on a magnetic nanoparticle, the feasibility of manipulation on bio-functionalized magnetic nanoparticles by external magnetic fields is investigated

Ex Vivo Magnetofection with Magnetic Nanoparticles: A Novel Platform for Nonviral Tissue Engineering

Several methods have been described to introduce DNA expression vectors into mammalian cells both in vitro and in vivo. Each system has benefits and limitations, and to date there is still no ideal method for gene transfer. In this study, we introduced a novel method of gene transfer by using Fe3O4 nanoparticles. The magnetic nanoparticles composed of Fe3O4, and the transfected genes used are Lac Z and enhanced green fluorescence protein gene (EGFG). Four different groups of preparations included in this study were homemade liposome-enveloped EGFP-DNA/Fe3O4, homemade liposome EGFP-DNA gene without magnetic Fe3O4 nanoparticles, lipofectamine 2000-enveloped EGFP-DNA, and EGFP-DNA gene only. Mice osteoblast and He99 lung cancer cell line were used as host cells for gene transfection. The time-dependent EGFP gene expression was monitored and analyzed. The results showed that the diameter of the complex was less than 100 nm. There was no cytotoxicity observed at any of the magnetic Fe3O4 nanoparticle concentrations tested. In the presence of magnetic field, the liposome-enveloped EGFP- DNA/Fe3O4 complex exhibited a much higher efficiency for transfecting EGFP-DNA into osteoblast cells under external magnetic fields. The gene can be transfected into cells with an aid of magnetic vectors and magnetic force. Under a gradient magnetic field, the efficiency of magnetofection is enhanced as compared to that without magnetic field

Dual Immobilization and Magnetic Manipulation of Magnetic Nanoparticles

By suitably bio-functionalizing the surfaces, magnetic nanoparticles are able to bind specific biomolecules, and may serve as vectors for delivering bio-entities to target tissues. In this work, the synthesis of bio-functionalized magnetic nanoparticles with two kinds of bio-probes is developed. Here, the stem cell is selected as a to-be- delivered bio- entity and infarcted myocardium is the target issue. Thus, cluster designation-34 (CD-34) on stem cell and creatine kinase-MB (CK-MB) (or troponin I) on infarcted myocardium are the specific biomolecules to be bound with bio-functionalized magnetic nanoparticles. In addition to demonstrating the co-coating of two kinds of bio-probes on a magnetic nanoparticle, the feasibility of manipulation on bio-functionalized magnetic nanoparticles by external magnetic fields is investigated

Surveillance on Waterfowl Avian Influenza

本計劃依各縣市水禽飼養比率及不同季節採集統計上足量之水禽血清樣品，分別以ELISA、AGP及HI tests測定禽流感抗體抗體並做亞型鑑定，抗體陽性場追蹤採糞材行反轉錄鋂禽流感之病毒檢測及亞型鑑定。Sera samples will be collected seasonally from all over the country. Avian influenza antibody of ELISA, agar gel precipitation (AGP), and hemagglutination inhibition (HI) test. Fecal samples will be collected from the antibody positive flocks, and then subjected to RT-PCR for AIV detection and subserotype determination

水禽及火雞類禽流感監控

Sera samples will be collected seasonally from all over the country. Avian influenza antibody of ELISA, agar gel precipitation (AGP), and hemagglutination inhibition (HI) test. Fecal samples will be collected from the antibody positive flocks, and then subjected to RT-PCR for AIV detection and subserotype determination. Domestic turkeys will detects for serological antiboies to avian influenza virus in Taiwan, sampling 20 from each group or farm to testing with enzyme-linked immunosorbent assay (ELISA). The antibody prevalence will be analyzing as a data for prevention of AI. The turkey farms showed positive reaction after serum detection will be test to viral isolation and sub-serotyping, this farm will going to sera monitoring.對水禽類動物鴨、鵝.雞及火雞共計265場，6000支肛門拭子或血清分別以應用反轉錄聚合