椎体融合器内固定治疗腰椎不稳症

目的　探讨后路椎体融合器内固定治疗腰椎不稳症的方法及疗效。方法　采用RF 2椎弓根内固定系统进行复位 ,撑开椎间隙后行椎管扩大减压 ,椎间盘髓核摘除、开孔 ,椎体融合器 (TFC)固定。结果　 6 3例经 5～ 38个月随访 ,症状消失 4 0例 ,明显减轻 17例 ,优良率 90 %。X线片显示椎间融合率 86 %。结论　使用TFC内固定治疗节段性腰椎间失稳症 ,能彻底摘除椎间盘组织 ,增加及维持椎间隙高度 ,增加椎管及椎间孔面积 ;恢复前中柱负重并可达到椎间即刻稳定 ,融合率高 ,且具有缩小手术范围、减少损伤、缩短手术时间和节约治疗费用等优

隐匿性重要动脉损伤36例诊断与治疗

目的　分析自 1995年 1月至 2 0 0 2年 5月收治隐匿性重要动脉损伤 36例的诊治经验。方法　早期诊断血管损伤 ,简捷有效的内固定 ,按损伤情况应用 Fogartyd导管取栓、血管修补、吻合、大隐静脉移植重建及放射介入技术球囊扩张导管阻塞动脉等处理方法。结果　优 :术后肢体血供正常、功能良好者 19例 ,占 5 2 .8%。可 :肢体部分肌肉坏死缺血性痉挛 ,跛行、足下垂 7例 ,小腿及足慢性骨髓炎、窦道 2例 ,占 2 5 .0 %。差 :肌肉坏死功能丧失 4例 ,占 11.1% ;肢体坏死截肢者 3例 ;肌肉坏死继发感染性休克死亡 1例。结论　在处理四肢创伤的同时应高度警惕隐匿性重要动脉损伤。只有早期诊断、及时探查、即刻修复受损血管 ,才能够获得满意的功能 ,恢复血流是非常重要

The Effects of the Combination of Ginsenoside Rg1,Cinnamic Acid and TanshinoneII A on the Proliferation and Genes Expression of MG-63 Cell

研究中药有效成分人参皂甙Rg1、肉桂酸和丹参酮IIA组合对人成骨肉瘤MG-63细胞增殖抑制和相关基因表达影响,探索其对肿瘤细胞的生物学效应。以33ìg/ml人参皂甙Rg1、296.32ìg/ml肉桂酸和0.3ìg/ml丹参酮IIA的组合(简称RCT)处理人成骨肉瘤MG-63细胞,以肿瘤细胞分化诱导物HMBA处理MG-63细胞为平行对照,用流式细胞仪、免疫细胞化学检测及光镜观察系统研究RCT组合对MG-63细胞的作用。生长曲线及细胞周期检测显示RCT组合可显著抑制MG-63细胞的增殖,细胞生长抑制率达72.37%,细胞周期阻滞于G0/G1期;免疫细胞化学检测显示RCT组合处理后MG-63细胞的癌基因c-fos、c-myc表达下调,抑癌基因p27、Rb表达上调。RCT组合对MG-63细胞增殖及相关基因表达的影响与分化诱导物六亚甲基双乙酰胺(HMBA)处理组相似。To explore the anticancer mechanisms and biological effects of the effective component of chinese herbs, the human osteosarcoma MG-63 cells were treated with the combination of 33 ìg/ml ginsenoside Rg1, 296.32 ìg/ml cinnamic acid and 0.3 ìg/ml tanshinoneIIA(RCT) and the MG-63 cells treated with hexamethylene bisacetamide(HMBA) were investigated as positive control of induced differentiation.They were subjected to flow cytometry, immuncocytochemical assay and light microscopy.The analysis of cell growth curve and cell cycle revealed that RCT can markedly inhibit cell proliferation and arrest cells in G0/G1 phase.The inhibitory rate of cell growth was 72.37% after 7 days treatment by RCT.The results of immunocytochemical staining indicated that the expression of oncogenes including c-fos and c-myc were down-regulated, whereas the expression of antitumor genes including p27 and Rb were up-regulated.Similar results were obtained from HMBA treated MG-63 cells.国家自然科学基金(No.30470877,No.20773100);; 福建省自然科学基金(No.2008J0302);; 中国博士后科学基金(No.20070420754)资助~

研發偵測堆肥中靈芝菌的選擇性培養基

The purpose of this study was to develop a selective medium for detecting Ling-Chih, Ganoderma lucidum (Leyss. ex Fr.) Karst. in compost. In 2009, two isolates GL1 and GL3 of G. lucidum acquired from a Chiayi mushroom cultivation field were used to conduct the research. GLS (Ganoderma lucidum selective) medium was developed by amendment of 10% V-8 vegetable juice agar (1 L) with 2 g thiabendazole (40% ai), 200 mg metalaxyl (35% ai), 200 mg streptomycin sulfate, 200 mg chloramphenicol, 1 mg PCNB (75% ai), 1.25 g tannic acid, 10 ml ethanol (95% v/v), and 2 ml lactic acid (85% v/v). The GLS medium rapidly detected G. lucidum in the compost within 2-3 days. Amendment of the medium with tannic acid induced G. lucidum to produce brown halo surrounding the colony as a selective feature. The multiple-pellet soil-sampler method was used to evaluate the sensitivity of GLS medium. The GLS medium was able to detect G. lucidum in the spent sawdust substrate which were 1000-fold diluted in disinfected sawdust substrate. This study concluded that the GLS medium could detect the survival of G. lucidum in the compost, and inhibit the growth of other fungi. Thus, GLS medium was recommended for manufacturers to determine if the compost is a suitable fertilizer.本研究主要目的在於研發靈芝菌的選擇性培養基，以便偵測廢棄的蕈菌生長基質調製成之堆肥或介質中是否仍有靈芝菌的存活。西元2009年，由嘉義取得的靈芝［Ganoderma lucidum （Leyss. ex Fr.）Karst.］太空包基質中分離GL1與GL3兩株靈芝菌菌株，進而評估七種真菌培養基、十種殺菌劑及兩種抗生素等對兩靈芝菌菌絲生長的影響，藉以研發靈芝菌的選擇性培養基。靈芝菌選擇性培養基（GLS）係利用10% V-8培養基作為基礎基質，並添加2 gL^(-1)腐絕（40% ai）、200 mg L^(-1)滅達樂（35% ai）、200 mg L^(-1)鏈黴素、200 mg L^(-1)氯黴素、1 mg L^(-1)五氯硝基苯（75% ai）、1.25 g L^(-1)單寧酸、10 mlL^(-1)酒精（95% v/v）及2 ml L^(-1)乳酸（85% v/v）配製而成。靈芝菌在GLS培養基生長2-3天可看到白色菌落，且於菌落周圍出現褐色反應。利用多重土丸取樣法分析含有不同靈芝接種源濃度之太空包木屑基質，結果顯示由台中及嘉義取得之靈芝太空包基質以無菌之太空包基質稀釋1000倍後，GLS培養基仍可偵測到G. lucidum的存活。本研究證明GLS培養基，除可自廢棄已久的太空包基質成功分離到靈芝菌外，尚可有效抑制其他雜菌的生長

凱利方格法在科學教育研究的應用 Application of Kelly’s Repertory Grid Technique in Research on Science Education

本研究以文獻回顧的方法探索凱利方格法的原理與策略，再反思科學教育研究領域中可應用凱利方格法進行研究的契機與限制。凱利方格法的分析與探索主要包含下列階段：元素與構念的萃取、方格的建構與評比，以及方格的分析與解釋。這些資料分析的過程對教育研究而言具備以下優勢。首先，研究者透過凱利方格法的應用，建立有系統的資料蒐集與彙整架構，發現資料中既存的脈絡。再者，凱利方格法除了能針對單一個案所反映的方格評比進行探索與分析外，也容許跨個案的比較。在科學教育相關研究中，這些優勢適合被應用於探索學生科學學習、教師專業發展，以及評鑑科學課程與教科書等。本研究最後也說明在應用凱利方格法分析時須考量的信度與效度，以及相關的研究限制，以更明確地說明使用凱利方格法分析的策略。 This paper discusses the possible application of the repertory grid technique (RGT) in science education through a literature review. We first describe the common strategies for using RGT in psychology and education research. We then explore the reasons for using RGT and the relationship between the aforementioned strategies and the purposes of studies in the literature. Finally, we explain whether and how using RGT can assist researchers in deeply exploring science education. Three basic strategies for using RGT are summarized according to a literature review: eliciting elements and constructs, constructing the grid, and analyzing the grid. RGT enables researchers to construct and reconstruct the qualitative data on the basis of quantitative analysis, which supports exploring subjects’ cognitive systems and comparing subjects. In science education, studies have applied RGT for various purposes, which we categorized into three types: exploring individual cognitive systems, developing principles for teachers’ professional development, and evaluating the quality of textbooks. The limitations and validations of RGT are discussed in the final section of this paper