Resistance Assessment for Oxathiapiprolin in Phytophthora capsici and the Detection of a Point Mutation (G769W) in PcORP1 that Confers Resistance

The potential for oxathiapiprolin resistance in Phytophthora capsici was evaluated. The baseline sensitivities of 175 isolates to oxathiapiprolin were initially determinated and found to conform to a unimodal curve with a mean EC50 value of 5.61×10-4 μg/ml. Twelve stable oxathiapiprolin-resistant mutants were generated by fungicide adaption in two sensitive isolates, LP3 and HNJZ10. The fitness of the LP3-mutants was found to be similar to or better than that of the parental isolate LP3, while the HNJZ10-mutants were found to have lost the capacity to produce zoospores. Taken together these results suggest that the risk of P. capsici developing resistance to oxathiapiprolin is moderate. Comparison of the PcORP1 genes in the LP3-mutants and wild-type parental isolate, which encode the target protein of oxathiapiprolin, revealed that a heterozygous mutation caused the amino acid substitution G769W. Transformation and expression of the mutated PcORP1-769W allele in the sensitive wild-type isolate BYA5 confirmed that the mutation in PcORP1 was responsible for the observed oxathiapiprolin resistance. Finally diagnostic tests including As-PCR and CAPs were developed to detect the oxathiapiprolin resistance resulting from the G769W point mutation in field populations of P. capsici

Transport and Mobility Needs for an Ageing Society from a Policy Perspective : Review and Implications

Funding: This research received no external funding.Peer reviewedPublisher PD

Exploring Differences in Commuting Behaviour among Various Income Groups during Polycentric Urban Development in China : New Evidence and Its Implications

Peer reviewedPublisher PD

Computer Simulation of Bioprocess

Bioprocess optimization is important in order to make the bioproduction process more efficient and economic. The conventional optimization methods are costly and less efficient. On the other hand, modeling and computer simulation can reveal the mechanisms behind the phenomenon to some extent, to assist the deep analysis and efficient optimization of bioprocesses. In this chapter, modeling and computer simulation of microbial growth and metabolism kinetics, bioreactor dynamics, bioreactor feedback control will be made to show the application methods and the usefulness of modeling and computer simulation methods in optimization of the bioprocess technology

Subway systems and tourism : An overview and implications

Peer reviewe

Fate of warm Pacific water in the Arctic Basin

Author Posting. © American Geophysical Union, 2021. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geophysical Research Letters 48(20), (2021): e2021GL094693, https://doi.org/10.1029/2021GL094693.Pacific Summer Water (PSW) plays a critical role in the ecosystem of the western Arctic Ocean, impacting sea-ice melt and providing freshwater to the basin. Most of the water exits the Chukchi Sea shelf through Barrow Canyon, but the manner in which this occurs and the ultimate fate of the water remain uncertain. Using an extensive collection of historical hydrographic and velocity data, we demonstrate how the PSW outflow depends on different wind conditions, dictating whether the warm water progresses eastward or westward away from the canyon. The current carrying the water westward along the continental slope splits into different branches, influenced by the strength and extent of the Beaufort Gyre, while the eastward penetration of PSW along the shelfbreak is limited. Our results provide the first broad-scale view of how PSW is transferred from the shelf to the basin, highlighting the role of winds, boundary currents, and eddy exchange.Funding for the project was provided by National Science Foundation grant OPP-1733564 and National Oceanic and Atmospheric Administration grant NA14OAR4320158 (P. Lin, R. S. Pickart, J. Li), and Trond Mohn Foundation Grant BFS2016REK01 (K. Vage).2022-04-0