Inductive effect of multiple cytokines on bone regeneration

临床上对创伤、感染和肿瘤切除后所造成大范围骨缺损的修复至今未得到有效的解决。在移植物材料中的生物活性物质(主要为细胞因子)能对其周围组织及长入移植物中的纤维组织发生诱导作用,使其向骨组织方向生长,从而促进骨缺损区的修复。国内外研究报道多种生物活性因子具有诱导成骨细胞的生长,抑制破骨细胞之间、破骨细胞与基质之间的黏附,具有诱导骨再生的能力。文章总结近年来细胞因子对诱导骨再生修复的影响及其进展,为具有骨诱导作用的细胞因子进行骨再生修复研究奠定基础。The reparation of extensive bone defects caused by the excision of trauma, infection and tumor on clinic has not been resolved availably.The biological active substances (mainly cytokines) in the graft material had inductive effect on the surrounding tissue and ingrowing fibrous tissue, which could direct the growth to the bone tissue, so as to promote the restoration of bone defects.Studies reported that a wide range of biologically active factor has the capacity of inducing regeneration of the bone by inducing the osteoblasts growth, and inhibiting the adhesion between osteoclasts, or between osreoclast and matrix.This paper summarized the effect of multiple cytokines on bone regeneration, which can provide a basis for the research of Inductive effect of multiple cytokines on bone regeneration

Experimental study of using GSPS for in situ osteogenesis induction on rabbit skull

目的:研究明胶-硅氧烷多孔支架材料混合多种材料用以原位诱导兔颅骨再生的效果。方法:采用可降解、具有双孔径的明胶-硅氧烷多孔支架材料,并复合bMP2和兔骨髓间充质干细胞,在去骨瓣减压手术时将材料置于骨窗上,同时采用空白对照来探讨兔颅骨原位诱导再生。用高速螺旋CT扫描颅骨并三维重建来测量颅骨缺损的愈合情况,并作出比较。结果:支架材料+bMP组动物的颅骨修复率与支架材料+红骨髓组动物的修复率相当(P=0.43);支架材料+红骨髓组动物的颅骨修复率高于单纯支架材料组动物的修复率(P=0.01);单纯支架材料组动物的颅骨修复率与空白对照组动物的修复率无明显差别(P=0.18)。结论:明胶-硅氧烷-CA(nO3)2多孔复合支架材料分别与bMP以及红骨髓混合使用能够明显提高颅骨缺损的骨愈合速度以及修复率;而单纯使用支架材料并不能促进实验动物的颅骨修复。Objective:To investigate the effect of using GSPS for in situ osteogenesis induction on rabbit skull.Methods:The biodegradable,with double-aperture gelatin -siloxane porous scaffold materials was mixed with BMP2 or rabbit bone marrow mesenchymal stem cells to fix the skull bone deficit after craniectomy decompression.A blank control was set to compare with the effect of in situ osteogenesis of rabbit skull.High-speed spiral CT scanning and three-dimensional reconstruction of the skull was performed to inspect the healing progress of skull defects.Results:The GSPS + BMP group had the equal bone healing rate as the GSPS+ bone marrow group (P=0.43); and the GSPS + bone marrow group had the higher bone healing rate than the GSPS group (P =0.01).There were no significant difference on bone healing rate between GSPS group and blank control group (P=0.18).Conclusion:Gelatin-siloxane-Ca (NO3) 2 porous scaffolds could be used with the BMP,as well as red bone marrow to improve the bone healing of skull bone defect while no significant effect could be observed using GSPS solely

Traumatic superficial temporal artery aneurysm:analysis of 7 cases

目的探讨外伤性颞浅动脉瘤的诊断和治疗方法。方法回顾性分析7例外伤性颞浅动脉的临床表现、手术方法和治疗结果。所有病例均因外伤后额部或颞部进行性增大肿物就诊,无颞浅动脉瘤破裂出血病例,全部病例在局部麻醉下切除动脉瘤囊肿,其中1例为分叉部梭形动脉瘤。结果病理报告均为假性动脉瘤,动脉外膜存在。无术后头皮缺血、坏死发生。结论根据外伤史、颞浅动脉走行上搏动性肿物的特点,颞浅动脉瘤即可明确诊断。动脉壁中膜弹力层断裂,局部因动脉血流搏动冲击膨出形成可能是颞浅动脉瘤形成的另一个机制。动脉瘤切除术是治疗颞浅动脉瘤的有效方法。Objective To explore the diagnosis and treatment for traumatic superficial temporal artery pseudoaneurysm.Methods Clinical manifestations, operation and prognosis of 7 patients with traumatic superficial temporal artery pseudoaneurysm were analyzed retrospectively.There was the same complaint of progressive swelling mass on the frontal or temporal area post brunt injury, but no complaint of rupture of the aneurysm in all the patients.All the patients underwent resections of the aneurysm capsule under local anesthesia, including one fusiform aneurysm at the bifurcation of the superficial temporal artery in 1 case.Results All the cases were pseudoaneurysm with intact outlayer of the artery.There was no complication of scalp ischemia or necrosis.Conclusion Traumatic superficial temporal artery pseudoaneurysm can be diagnosed according to the history of head injury and progressive pulsatile swelling or mass on the line of the superficial temporal artery.It may be another mechanism of forming pseudoaneurysm that breakdown of the elastic layer in the tunica media of the arterial wall and beating of arterial blood flow arterial lead to lumen enlargement on the local point.Resection of the aneurysm is an effective way of the treatment

伴有癫癇发作的脑内病灶的手术治疗

Objective To study the surgical outcome of intracerebral lesion with epilepsy. Methods 24 cases were studied. EEG showed that localized epileptiform waves were found in 13 cases, localized slow waves in 5 cases, and no epileptiform wave in 6 cases. All cases were performed ECoG monitoring during the operations. 11 patients whose epileptogenic foci were in the surface of lesions or adjacent to the edge of lesions received clearance of focal lesion plus epileptogenic foci resection. Among 8 patients whose epileptogenic foci were more than 10 mm distance from the edge of lesion, 6 received clearance of focal lesion plus cortical excision whose epileptogenic foci were in the area of nonfunctional cortexes, 2 plus partial cortex thermocoagulation whose epileptogenic foci were in the area of functional cortexes. 2 cases whose epileptogenic foci in the depth of anterior temporal lobe received anterior temporal lobotomy plus clearance of focal lesion. 3 patients with no epileptiform wave received clearance of focal..

Biological N_2 fixation rates in the East China Sea in spring 2009

2009年4~5月间,应用15n2示踪法实测了东海25个站位的生物固氮速率,结合温度、盐度等要素的分布,分析生物固氮速率的空间变化及其影响因素.结果表明,春季东海的生物固氮速率介于102~16 349 nMOl/(M3·d)之间,平均为2 441 nMOl/(M3·d).生物固氮速率的空间变化呈现径流影响区最高,黑潮影响区与陆架其他区域相近的特点,径流影响区的平均生物固氮速率约为黑潮影响区和陆架其他区域的2倍,这可能与固氮生物种类的空间变化有关.春季东海的积分固氮速率平均为129μMOl/(M2·d),结合此前获得的夏、秋、冬季的积分固氮速率,估算出东海生物固氮速率的年均值为50μMOl/(M2·d),因而东海的年固氮通量估计为1.4x1010MOl/A,约占全球海洋年固氮通量的0.15%.对东海进行的氮源汇分析表明,生物固氮作用、长江径流输入和大气沉降输入等氮输入通量分别为1.4x1010、7.9x1010和3.9x1010MOl/A,反硝化作用和沉积物颗粒氮埋藏的迁出通量分别为9.5x1010和3.7x1010MOl/A,东海氮的源汇通量均为13.2x1010MOl/A,表明东海氮的收支基本处于平衡状态.Biological N2fixation rates at 26 stations in the East China Sea were measured with15N2tracer assay during April and May 2009.Combined with temperature and salinity,the distribution of N2fixation rates and their influencing factors were discussed.Our results showed that the N2fixation rates ranged from 102 to 16 349 nmol/(m3·d) with an average of 2 441 nmol/(m3·d).The highest rates were observed in regions influenced by the river plume,which was about two times what observed in regions influenced by the Kuroshio current and the shelf waters,probably reflecting the spatial variation of the nitrogen fixers.The integrated N2fixation rate in the East China Sea in spring was129 μmol/(m2·d) in average,and the annual N2fixation rate was estimated as 50 μmol/(m2·d) compared with previous data in summer,autumn and winter.This meant that the new nitrogen flux supply by N2fixation to the East China Sea was 1.4×1010mol/a,contributing to about 0.15% of the N2fixation flux in global oceans.The nitrogen budget in the East China Sea was evaluated in views of sources and sinks.The fluxes of nitrogen supply to the East China Sea via the nitrogen fixation,the river runoff and the atmospheric deposition were estimated as 1.4×1010,7.9 ×1010and 3.9×1010mol/a,respectively.The removal fluxes of nitrogen via denitrification and sedimentary burial were 9.5×1010and 3.7×1010mol/a,respectively.Both the nitrogen flux of the sources and the sinks were 13.2×1010mol/a,indicating a balance of nitrogen budget in the East China Sea.国家自然科学基金杰出青年基金资助项目(41125020); 国家海洋局大洋专项资助项目(DY125-13-E-01