Autophagy Inhibition by Chloroquine Sensitizes Cervical Cancer SiHa Cells to CPT Treatment

自噬诱导是肿瘤细胞对化疗药物抵抗性的原因之一,该研究探讨溶酶体抑制剂氯喹对喜树碱(camptothecin,CPT)诱导的宫颈癌细胞Si Ha死亡的增敏效果。CPT和/或氯喹处理宫颈癌Si Ha细胞,MTT法检测细胞增殖,DAPI和TUNEL染色观察细胞凋亡,Western blot和免疫荧光检测自噬及凋亡相关蛋白。结果发现,CPT处理后,Si Ha细胞MAP1LC3B荧光点和LC3II(microtubuleassociated protein light chain 3II)蛋白水平增加,p62荧光点和蛋白质水平则减少;而采用氯喹特异抑制自噬后,可明显提高CPT诱导的细胞凋亡、caspase-9的激活和PARP(poly ADP-ribose polymerase)的切割,而全长caspase-2水平显著下降。以上结果提示,氯喹可通过抑制细胞自噬而增强宫颈癌细胞株Si Ha对CPT诱导细胞凋亡的敏感性。The autophagy induction is one of the reasons for the resistance of tumor cells to chemotherapy drugs. In this study, the enhanced sensitivity of cervical cancer Si Ha cells to camptothecin(CPT)-induced cell death by chloroquine(a lysosome inhibitor) was investigated. The cell viability was detected by MTT assay, meanwhile, apoptosis was observed by DAPI and TUNEL, autophagy related proteins and apoptosis proteins were analyzed by immunofluorescence(IF) staining and Western blot in Si Ha cells after CPT treatment alone or combined with chloroquine. The results found that in Si Ha cells with CPT treatment the autophagy related protein LC3 foci and microtubule-associated protein light chain 3II(LC3II) protein level was increased, but p62 foci and protein level was decreased. When autophagy was inhibited by chloroquine, the CPT-induced apoptosis was obviously enhanced, and caspase-9 was activated and PARP was cleaved, but full length caspase-2 was decreased. Taken together, these results indicated that the inhibition of autophagy by chloroquine could sensitize cervical cancer Si Ha cells to CPT inducing cell apoptosis.山西省自然科学基金(批准号:2014021037-9);; 山西医科大学汾阳学院博士启动基金(批准号:1301);山西医科大学汾阳学院科研项目基金(批准号:1422)资助的课题~

基于内毒素血症探讨皂术茵陈方对非酒精性脂肪性肝炎大鼠的影响

目的 基于内毒素血症探讨皂术茵陈方防治非酒精性脂肪性肝炎(NASH)大鼠的影响及其作用机制。方法 采用随机数字表法将40只大鼠分为5组,即正常组、模型组、皂术茵陈方组、盐酸吡格列酮组及培菲康组,每组8只。除正常组外,其余32只大鼠采用高脂饮食16周建立大鼠NASH模型,在造模第9周开始,皂术茵陈方组给予皂术茵陈方水提液60 mg/(kg·d)灌胃,盐酸吡格列酮组给予盐酸吡格列酮10 mg/(kg·d)灌胃,培菲康组给予培菲康210 mg/(kg·d)灌胃,正常组及模型组均给予双蒸水10 mL/(kg·d)灌胃,共治疗8周。第16周末经腹主动脉取血,生化法检测肝组织甘油三酯(TG)水平,苏木精—伊红(HE)染色观察肝组织病理学变化。终点显色法检测血浆内毒素(LPS)含量。ELISA法检测肝组织肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6等炎症因子的表达水平。结果 与正常组比较,模型组大鼠肝组织显示出典型的NASH组织学特征,出现重度脂肪变性,不同程度的炎细胞浸润和坏死灶。大鼠肝湿重、肝指数、肝脏TG含量、血浆LPS水平、肝组织TNF-α、IL-1β、IL-6等炎症因..

CCCCC pentadentate chelates with planar Möbius aromaticity and unique properties

本课题充分发挥了厦门大学多学科协同研究优势，通讯作者为夏海平教授（合成、表征）、刘刚教授（生物医学应用）和吕鑫教授（理论计算）。合成实验和结构表征由朱从青（第一作者，目前在麻省理工学院、2005年诺贝尔化学奖得主Richard Schrock教授课题组从事博士后研究）完成；生物医学应用由杨彩霞（共同第一作者）、林凎、杨宇惠、王晓勇合作完成；理论计算由朱军、王永恒、朱从青完成。美国NIH的陈小元教授参与了生物医学应用的讨论。该研究工作得到国家自然科学基金委、科技部项目的支持。The coordinating atoms in polydentate chelates are primarily heteroatoms. We present the first examples of pentadentate chelates with all binding atoms of the chelating agent being carbon atoms, denoted as CCCCC chelates. Having up to five metal-carbon bonds in the equatorial plane has not been previously observed in transition metal chemistry. Density functional theory calculations showed that the planar metallacycle has extended Craig-Möbius aromaticity arising from 12-center–12-electron dπ-pπ π-conjugation. These planar chelates have broad absorption in the ultraviolet-visible–near-infrared region and, thus, notable photothermal performance upon irradiation by an 808-nm laser, indicating that these chelates have potential applications in photothermal therapy. The combination of facile synthesis, high stability, and broad absorption of these complexes could make the polydentate carbon chain a novel building block in coordination chemistry.the National Basic Research Program of China (nos. 2012CB821600 and 2014CB744503) , the National Science Foundation of China (nos. 21332002, 81422023, 51273165, 21490573, and 21573179)

改良分子信标-双重实时荧光PCR快速检测SARS病毒

目的建立改良分子信标-双重实时荧光PCR检测SARS病毒的方法,用于SARS的早期诊断和动物溯源。方法利用改良分子信标技术、装甲RNA和双片段双色荧光技术,根据GenBank公布的SARS病毒聚合酶基因1b的阅读开放框架结构的保守序列,自行设计一对引物和探针,以部分临床标本的酶联吸附实验结果和传统细胞培养方法作为对照,建立分子信标检测SARS病毒的方法。对368份临床标本(咽漱液、血液、粪便、尿液)、52份细胞培养液和50份动物标本进行荧光PCR扩增。结果分子信标检测SARS病毒的方法灵敏度为10～100个拷贝ml,与流感病毒等呼吸道病毒无交叉反应。分子信标检测368份临床标本,20份阳性。其中确诊病例阳性率为21.27%(1047),确诊病例的咽漱液阳性率为43.48%,还分别从粪便和血清中检测到SARS病毒。52份细胞培养液,29份阳性,阳性率为55.77%。50份动物标本,23份阳性,阳性率为46%。结论改良分子信标-双重实时荧光PCR检测SARS病毒方法灵敏度高、特异性强,可用于SARS的临床早期诊断和动物溯源

Freestanding palladium nanosheets with plasmonic and catalytic properties

通讯作者地址: Huang, XQ (通讯作者), Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 3. Xiamen Univ, Dept Phys, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] metal films can exhibit quantum size and surface effects that give rise to unique physical and chemical properties(1-7). Metal films containing just a few layers of atoms can be fabricated on substrates using deposition techniques(7), but the production of freestanding ultrathin structures remains a significant challenge. Here we report the facile synthesis of freestanding hexagonal palladium nanosheets that are less than 10 atomic layers thick, using carbon monoxide as a surface confining agent. The as-prepared nanosheets are blue in colour and exhibit a well-defined but tunable surface plasmon resonance peak in the near-infrared region. The combination of photothermal stability and biocompatibility makes palladium nanosheets promising candidates for photothermal therapy. The nanosheets also exhibit electrocatalytic activity for the oxidation of formic acid that is 2.5 times greater than that of commercial palladium black catalyst.NSF of China 20925103 20871100 20721001 20703032 MOST of China 2009CB930703 2011CB932403 Fok Ying Tung Education Foundation 121011 NSF of Fujian 2009J06005 Key Scientific Project of Fujian Province 2009HZ0002-

Research on the Particular VP for the English Generation of Chinese-English Translation System

主要讨论汉英两种语言特殊动词短语表示方式的不同，包括动词短语的拆分、变形和辅助成分的确定。在分析模块给出的中间语言信息不够详尽的情况下，需要生成模块针对性地作进一步处理，才能得到规范准确的译文。生成模块中一个详尽的词典非常重要，提出的生成策略就是在一个完备的词典的基础上通过规则来实现的。This article mostly discusses the difference of expression of special VP between Chinese and English, including the split, distortion and assistant ingredient's confirming of VP. When the informations of interlingua given by the analysis model is not elaborate, we have to deal with the generation model centralizly and ulteriorly to get the normative and correct translation. A elaborate lexicon is very important in the generation model. The generation tactic given by this article is realized by rules on the foundation of a elaborate lexicon.国家“863”资助项目!(863-306-ZT03-06-1

Research on Interrogative Information in Interrogative Sentence for Chinese-English Translation

汉语的疑问句的提问信息分布于句中，不像英语那样是置于句首的，因此汉语疑问句的关键提问信息(即疑问点)的分析就成为我们需研究的主要问题。首先总结了汉语疑问句疑问结构的类型，然后提出了采用上下文无关的合一规则作为分析手段的总体思路，并针对疑问结构的各类型给出相应的分析规则及算法。实验证明本文提出的分析策略具有较好的处理效果。The interrogative information is hidden in Chinese interrogative sentences, but in English it is placed at the head of the sentence. So the parsing for the key interrogative information, which we call interrogative point, becomes our research focus. In this pager, we summary the category of the structure of interrogative point in Chinese interrogative sentences. Then we propose to take content free unification rules as our parse method, and give the according rules and algorithms for the different interrogative point category. The parse method is proved to have better performance in the experiments.国家“863”计划资助项目!(863-306-ZT03-06-1

Example-based Chinese Syntactic Structure Disambiguation

本文论述了一种基于实例的汉语句法结构分析的消歧方法。本文首先提出了这种方法的总体思路 ,并对其语义知识资源—《知网》作了简要的介绍。然后详细地描述了基于实例的排歧法的主要算法。最后给出的算法实验结果例子证明 ,这种方法是对汉语的结构分析排歧是有效的。This paper presents a description of the method of example based Chinese syntactic structure disambiguation method.First we put forward the general idea about this method and give a brief introduce to its semantic knowledge resource the Hownet Dictionary.Then the main algorithm for the method of example based disambiguation is proposed with detail.The experiment result given in the end proves our method to be effective.国家 8 6 3课题!(86 3- 30 6 -ZT0 3- 0 6 - 1

The pressure probe technique: principles and application in research of plant water relations

现代压力探针主要分为高等植物细胞压力探针、根压力探针、木质部压力探针和取样压力探针4个变种,并形成了相关的理论体系,广泛应用于植物生理学及其相关领域的研究中。现代压力探针技术不仅可用于高等植物细胞和组织水平水分关系、水和溶质的相互作用关系的研究,还可进行根木质部负压的测定及高等植物活体单细胞内含物的取样研究等。未来更可借助取样压力探针与微量分析技术的结合,将高等植物单细胞内的水分关系和代谢过程定量联系起来;或可试图拓宽木质部压力探针测定的压力范围,进一步研究和验证内聚力-张力学说的理论和机制