Ethical Reflection on Standardized Management of Intestinal Microbiota Transplantation

肠道微生态系统是人体健康所必需的重要且复杂的生物系统。肠道微生态系统的紊乱参与人体多种疾病的发生发展过程。而肠菌移植技术可有效调节肠道菌群，修复失衡的肠道微生态，为当今多种胃肠道及胃肠以外疾病的治疗带来新的突破。然而，目前尚缺乏完整的、系统性的肠菌移植技术管理规范。就肠菌移植技术规范化管理所涉及的内容进行探讨，并从医学伦理学角度思考规范化管理中所涉及的伦理问题，以期推动肠菌移植技术的临床应用。Intestinal microecology is an important and complex biological system necessary for human health. Its disorder is involved in the development of various diseases of human body. The technology of intestinal microbio- ta transplantation can effectively regulate the intestinal flora, repair the imbalance of the intestinal microecology, and bring a new breakthrough for the treatment of many diseases of gastrointestinal tract and outside gastrointestinal tract. However, there is still no systematic and complete management standard for intestinal microbiota transplanta- tion technology. This paper discussed related content involved in standardized management of intestinal microbiota transplantation technology and reflected the ethical problems involved in standardized management from the perspec- tive of medical ethics, in order to promote the clinical application of intestinal microbiota transplantation technology

Traumatic superficial temporal artery aneurysm:analysis of 7 cases

目的探讨外伤性颞浅动脉瘤的诊断和治疗方法。方法回顾性分析7例外伤性颞浅动脉的临床表现、手术方法和治疗结果。所有病例均因外伤后额部或颞部进行性增大肿物就诊,无颞浅动脉瘤破裂出血病例,全部病例在局部麻醉下切除动脉瘤囊肿,其中1例为分叉部梭形动脉瘤。结果病理报告均为假性动脉瘤,动脉外膜存在。无术后头皮缺血、坏死发生。结论根据外伤史、颞浅动脉走行上搏动性肿物的特点,颞浅动脉瘤即可明确诊断。动脉壁中膜弹力层断裂,局部因动脉血流搏动冲击膨出形成可能是颞浅动脉瘤形成的另一个机制。动脉瘤切除术是治疗颞浅动脉瘤的有效方法。Objective To explore the diagnosis and treatment for traumatic superficial temporal artery pseudoaneurysm.Methods Clinical manifestations, operation and prognosis of 7 patients with traumatic superficial temporal artery pseudoaneurysm were analyzed retrospectively.There was the same complaint of progressive swelling mass on the frontal or temporal area post brunt injury, but no complaint of rupture of the aneurysm in all the patients.All the patients underwent resections of the aneurysm capsule under local anesthesia, including one fusiform aneurysm at the bifurcation of the superficial temporal artery in 1 case.Results All the cases were pseudoaneurysm with intact outlayer of the artery.There was no complication of scalp ischemia or necrosis.Conclusion Traumatic superficial temporal artery pseudoaneurysm can be diagnosed according to the history of head injury and progressive pulsatile swelling or mass on the line of the superficial temporal artery.It may be another mechanism of forming pseudoaneurysm that breakdown of the elastic layer in the tunica media of the arterial wall and beating of arterial blood flow arterial lead to lumen enlargement on the local point.Resection of the aneurysm is an effective way of the treatment

魁蚶(Scapharca broughtonii)热休克蛋白90(HSP90)基因的克隆及转录表达分析

热休克蛋白90(HSP90)作为一种研究的常规免疫基因,在许多物种都有过报道。本研究从构建的魁蚶转录组文库中筛选得到的HSP90基因部分序列为基础,通过RACE技术获得其c DNA全长序列(命名为Sb HSP90),以期明确魁蚶HSP90基因的结构特征、组织分布及其对病原菌刺激的免疫变化规律。序列和结构分析表明,该c DNA全长2707bp,编码一个由728个氨基酸组成的多肽,该多肽含有HSP90家族共有的5个签名序列,C端高度保守的MEEVD短肽序列和ATPase结构域;预测蛋白的分子量(Mw)为83.72k Da,理论等电点(p I)为4.85;预测该蛋白无信号肽,具有4个糖基化位点。同源性及系统分析表明,Sb HSP90基因与软体动物的HSP90相似性达到83%以上,其中与长牡蛎(Crassostrea gigas)和海湾扇贝(Argopecten irradians)相似度最高达86%,与甲壳动物HSP90的相似度都在81%左右,与脊椎动物HSP90-α和HSP90-β的同源性都很接近。实时荧光定量PCR(q RT-PCR)结果表明:Sb HSP90 m RNA在魁蚶血细胞、斧足、鳃、外套膜、闭壳肌和肝胰腺和中均有表达,斧足中的表达量相对较高,而在肝胰腺中的表达量则相对较低;注射鳗弧菌后,相对于对照组,Sb HSP90基因在所检测的每个组织中m RNA水平上的表达量都显著上调(P<0.01),而且具有显著的时间依赖性和瞬时表达趋势。国家自然科学基金项目,31602142号;;中国水产科学研究院基本科研业务费项目,2017GH07号;;浙江省重中之重学科开放基金,KF2015007

凡口铅锌矿湿地处理系统的土壤原生动物

20 0 1年 12月 ,在广东省韶关市凡口铅锌矿湿地处理系统中采集土壤样品 ,采用Singh和Stout的培养法对土样中的原生动物进行定性、定量研究。共发现原生动物 4 2种 ,对照区和湿地每克风干土壤中原生动物的平均丰度分别为 2 377和 113,三大类土壤原生动物丰度中鞭毛虫占优势 ,多于纤毛虫和肉足虫。通过原生动物种类、丰度与土壤理化参数的统计分析说明铅锌尾矿对土壤原生动物群落的危害极大。通过土壤原生动物丰度和物种相似性聚类分析结果反映出正在使用的湿地 3土壤受污染严重 ,已停止使用的湿地 1

THE STUDY OF MAIN TECHNICAL PARAMETERS OF ELECTROCHEMICAL REGENERATING ACTIVATED CARBON

对所选定的活性炭 ,吸附苯酚饱和后进行电化学再生。在室温条件下 ,选择 4因素、2水平的正交试验方法进行了相关试验。结果表明 ,再生位置是活性炭再生工艺中最重要的影响因素 ,电解质NaCl浓度是较重要的影响因素 ,再生电流和再生时间对活性炭的电化学再生有一定的影响。Electrochemical regeneration of activated carbon adsorbed with phenol was investigated in different operative conditions.The effects of several operative parameters on electrochemical regeneration efficiency were measured in room temperature by means of orthogonal experimental method.The experimental results show that it is the key factor to put activated carbon on the cathode not the anode,and other main factors of effecting regeneration efficiency are as follows:the regeneration current intensity is 30mA,regeneration time is 5 hours,electrolyte(NaCl)concentration is 2%. [WT5HZ]国家自然科学基金!资助项目 (No.2 96760 3 5

伴有癫癇发作的脑内病灶的手术治疗

Objective To study the surgical outcome of intracerebral lesion with epilepsy. Methods 24 cases were studied. EEG showed that localized epileptiform waves were found in 13 cases, localized slow waves in 5 cases, and no epileptiform wave in 6 cases. All cases were performed ECoG monitoring during the operations. 11 patients whose epileptogenic foci were in the surface of lesions or adjacent to the edge of lesions received clearance of focal lesion plus epileptogenic foci resection. Among 8 patients whose epileptogenic foci were more than 10 mm distance from the edge of lesion, 6 received clearance of focal lesion plus cortical excision whose epileptogenic foci were in the area of nonfunctional cortexes, 2 plus partial cortex thermocoagulation whose epileptogenic foci were in the area of functional cortexes. 2 cases whose epileptogenic foci in the depth of anterior temporal lobe received anterior temporal lobotomy plus clearance of focal lesion. 3 patients with no epileptiform wave received clearance of focal..

利用爆轰产生高温燃气的方法

用高温燃气流不仅可以开展高超飞行器热环境地面模拟试验,和冲压发动机尾喷流特性及其对飞行器后体扰流的相互作用规律试验,而且可以通过产生不同燃气,研究燃气本身的不同对实验结果的影响规律(包括污染气体对发动机推力性能的影响)。目前国内外开展高温燃气流实验,采用的加热方式主要有燃烧加热、电弧加热、电磁加热、蓄能加热和激波加热等。这些加热方式都有各自的优点和局限性,有的加热温度不高、流场不均匀,有的带来很

Quantitative analysis of two new dominant species of macrozoobenthos in Xiamen Harbour,China

根据2004年在厦门海域22个取样站获得的4个季度的大型底栖动物调查数据以及历史资料,发现软体动物双壳纲的光滑河蓝蛤(Potamocorbula laevis)是厦门海域大型底栖动物群落的局部、季节性优势种;环节动物多毛纲的昆士兰稚齿虫(Prionospio queenslandica)是局部优势、周年常见种。春季和夏季,光滑河蓝蛤在鼓浪屿附近海域的密度分别为1098.8个/m2和1720.0个/m2,夏季,在曾厝安附近海域的密度为3773.3个/m2,均占所在海域大型底栖动物密度的50%以上,而冬季和秋季,很少采集到光滑河蓝蛤。夏季和秋季,昆士兰稚齿虫在鼓浪屿附近海域的密度分别为871.3个/m2和232.5个/m2;从季度平均值看,昆士兰稚齿虫密度和生物量夏季最高,秋季次之,春季第三,冬季最低;昆士兰稚齿虫的优势度没有光滑河蓝蛤高,但在整个厦门海域均有一定的密度和生物量。本研究分析了光滑河蓝蛤、昆士兰稚齿虫数量与有机质、硫化物含量、水动力等环境因子之间的关系。According to macrofaunal data got seasonally at 22 sampling stations in Xiamen Harbor in 2004 and history data,we found that Potamocorbula laevis (Bivalvia: Corbulidae) becomes a new local and seasonal dominant species,and Prionospio queenslandica (Polychaeta: Spionidae) becomes a new local dominant species and yearly common species. The density of Potamocorbula laevis was 1 098.8 ind./m2 and 1 720.0 ind./m2 in the sea water around Gulang Island in spring and summer respectively,and was 3 773.3 ind./m2 in the sea water near Zengcuo-an in summer. All the densities above exceed 50% of macrofaunal density. Potamocorbula laevis was few in winter and autumn. The densities of Prionospio queenslandica were 871.3 ind./m2 and 232.5 ind./m2 in the sea water around Gulang Island in summer and autumn respectively. The density of Prionospio queenslandica was the highest in summer,the second in autumn,the third in spring and the lowest in winter. The density of Prionospio queenslandica was not so higher than that of Potamocorbula laevis,but Prionospio queenslandic distributed in whole Xiamen sea water and in all seasons. In this paper,the relationships between the quantities of two species and contents of organic matter,sulfide,and hydrodynamic were analyzed