Isotope Tracers for Marine Processes in the North Pacific and the TongAn Bay

通过对北太平洋与同安湾表层水镭同位素（226Ra、228Ra、224Ra）含量与分布特征的研究，将其具体应用于揭示北太平洋亚热带环流区生态系对全球变化的响应以及同安湾海底地下水输入的影响。此外，借助北太平洋沉积物岩心放射性核素的分布，计算岩心的沉积速率及生物扰动速率。研究获得如下主要结果： 北太平洋表层水226Ra比活度、228Ra比活度和228Ra/226Ra)A.R随经度的变化均不大，且数值明显比河口港湾与边缘海的相应值来得低，呈现开阔大洋水的典型特征。结合文献报道的研究海域226Ra、SiO32-含量的历史数据，证实北太平洋亚热带环流区表层水226Ra比活度在过去40年中呈现降低趋势，...In this study, radium isotopes (226Ra, 228Ra and 224Ra) in the surface water of the North Pacific and the TongAn Bay were measured and used as tracers for the ecosystem shift of the North Pacific Subtropical Gyre in response to global change, and the impact of submarine groundwater discharge (SDG) in TongAn Bay. In addition, the sedimentary rate and bioturbation rate at one sediment core from the ...学位：理学硕士院系专业：海洋与环境学院海洋学系_海洋化学学号：20032701

海洋胶体中的氮、磷和铁对微藻生长的效应

利用错流超滤技术提取海洋胶体,研究了无氮、无磷和无铁的营养介质中海洋胶体对海水小球藻、亚心形扁藻和球等鞭金藻生长的影响。结果表明,在胶体有机碳(COC)浓度为0～238.4μmol/L的范围内,3种微藻在以胶体为N源的介质中的平均相对增长率Y分别为20.7％～44.0％,80.1％～110.1％和68.6％～106.2％;在以胶体为P源的介质中的Y值分别为19.3％～43.2％,78.3％～156.7％和77.9％～115.3％。3种微藻的Y值随COC浓度增大呈下降趋势。3种微藻在以胶体为Fe源的介质中的Y值分别为12.7％～35.3％,171.7％～266.3％和400.5％～498.8％,3种微藻的Y值随COC浓度增大呈线性上升。这意味着海洋胶体中存在微藻生长必需的N,P和Fe营养,对微藻的生长有显著的刺激作用。胶体中的Fe营养对球等鞭金藻和亚心形扁藻的效应比N和P营养的约大6～7倍,而胶体中的N和P营养对小球藻的促进作用则约比Fe的大3倍。国家教育部高等学校骨干教师计划基

魁蚶(Scapharca broughtonii)热休克蛋白90(HSP90)基因的克隆及转录表达分析

热休克蛋白90(HSP90)作为一种研究的常规免疫基因,在许多物种都有过报道。本研究从构建的魁蚶转录组文库中筛选得到的HSP90基因部分序列为基础,通过RACE技术获得其c DNA全长序列(命名为Sb HSP90),以期明确魁蚶HSP90基因的结构特征、组织分布及其对病原菌刺激的免疫变化规律。序列和结构分析表明,该c DNA全长2707bp,编码一个由728个氨基酸组成的多肽,该多肽含有HSP90家族共有的5个签名序列,C端高度保守的MEEVD短肽序列和ATPase结构域;预测蛋白的分子量(Mw)为83.72k Da,理论等电点(p I)为4.85;预测该蛋白无信号肽,具有4个糖基化位点。同源性及系统分析表明,Sb HSP90基因与软体动物的HSP90相似性达到83%以上,其中与长牡蛎(Crassostrea gigas)和海湾扇贝(Argopecten irradians)相似度最高达86%,与甲壳动物HSP90的相似度都在81%左右,与脊椎动物HSP90-α和HSP90-β的同源性都很接近。实时荧光定量PCR(q RT-PCR)结果表明:Sb HSP90 m RNA在魁蚶血细胞、斧足、鳃、外套膜、闭壳肌和肝胰腺和中均有表达,斧足中的表达量相对较高,而在肝胰腺中的表达量则相对较低;注射鳗弧菌后,相对于对照组,Sb HSP90基因在所检测的每个组织中m RNA水平上的表达量都显著上调(P<0.01),而且具有显著的时间依赖性和瞬时表达趋势。国家自然科学基金项目,31602142号;;中国水产科学研究院基本科研业务费项目,2017GH07号;;浙江省重中之重学科开放基金,KF2015007

近40年北太平洋亚热带环流区生态系变化的~(226)Ra证据

利用1999年7～10月和2003年10～12月实测的北太平洋亚热带环流区表层水~(226)Ra含量,结合文献所报道的历史数据,发现自20世纪60年代至今,北太平洋亚热带环流区表层水体~(226)Ra含量呈下降趋势,反映出全球变化导致该海域生态系结构的变化.全球增暖一方面导致了该海域水体层化作用的加强,进而导致上层水体Ra补充量的减少;另一方面,全球增暖导致海域生物生产力的升高,进而导致Ra迁出量的增加.~(226)Ra含量的上述变化与文献报道的该海域叶绿素a、硅酸盐、磷酸盐含量与初级生产力的历史变化趋势相吻合,确证北太平洋亚热带环流区在全球变化背景下正发生着可观测的生态系统变化.在上层海洋层化作用加强的情况下,为维持海域升高的生物生产力,其“新”氮可能的来源是海洋的固氮作用

伴有癫癇发作的脑内病灶的手术治疗

Objective To study the surgical outcome of intracerebral lesion with epilepsy. Methods 24 cases were studied. EEG showed that localized epileptiform waves were found in 13 cases, localized slow waves in 5 cases, and no epileptiform wave in 6 cases. All cases were performed ECoG monitoring during the operations. 11 patients whose epileptogenic foci were in the surface of lesions or adjacent to the edge of lesions received clearance of focal lesion plus epileptogenic foci resection. Among 8 patients whose epileptogenic foci were more than 10 mm distance from the edge of lesion, 6 received clearance of focal lesion plus cortical excision whose epileptogenic foci were in the area of nonfunctional cortexes, 2 plus partial cortex thermocoagulation whose epileptogenic foci were in the area of functional cortexes. 2 cases whose epileptogenic foci in the depth of anterior temporal lobe received anterior temporal lobotomy plus clearance of focal lesion. 3 patients with no epileptiform wave received clearance of focal..

Water masses in the Bering Strait revealed by radium isotopes

对白令海峡64.3°n纬向断面镭同位素的研究表明,水体中226rA比活度2、28rA比活度和228rA/226rA)A.r.存在明显的纬向变化,反映出太平洋与北冰洋水体交换的多种路径。根据温度、盐度和镭同位素的水平与垂直分布,太平洋水进入北冰洋的路径可能主要有3支,分别为白令海峡西侧的阿拉德水、白令海峡东侧的阿拉斯加沿岸水和中部的白令海陆架水。2003年7月和9月对该断面西侧站位进行的复测显示,水体中的226rA比活度2、28rA比活度和228rA/226rA)A.r.发生了明显变化,9月份测值均高于7月份测值,说明白令海峡西部水体在9月份可能受到西伯利亚沿岸流南向混合的影响。Radium isotopes in the water column along 64.3°N in the Bering Strait were determined to reveal the northward flow of the Pacific water.The specific activities of 226Ra and 228Ra,and the 228Ra/226Ra)A.R.show a longitudinal variation in the Bering Strait,depicting the pathways of Pacific inflow.Based on the distributions of temperature,salinity and radium isotopes in the Bering Strait,three water masses were identified: the Anadyr Water in the western channel,the Alaskan Coastal Water in the eastern channel,and the Bering Shelf Water in the central strait.A subseasonal variation of radium isotopes in the Bering Strait was observed.The specific activities of 226Ra and 228Ra,and 228Ra/226Ra)A.R.in September are higher than those observed in July,indicating water in the western Bering Strait is influenced by the southward flow of the Siberia Coastal Current in September.国际极地年中国行动计划项目;中国第3次北极科学考察资助项目;福建省自然科学基金杰出青年基金项目(2009J06026

THE EFFECT OF NATURAL COLLOIDS ON THE GROWTH OF ALGAE

利用错流超滤技术提取天然胶体,研究了胶体的有机碳浓度、粒径和来源对海水小球藻(Chlorellaspp.)、亚心形扁藻(Platymonassubcordiformis)和球等鞭金藻(Isochrysisgalbana)生长的影响。结果表明,当粒径为10kDa—0.2μm、胶体有机碳浓度为4.8—238.4μmol/L时,胶体使小球藻、扁藻和金藻的生长率分别提高了4.0%—7.0%、19.1%—28.9%和2.9%—8.7%。海水小球藻生长率的相对增长百分数(Y)值与胶体有机碳浓度(CCOC)呈线性正相关;亚心形扁藻和球等鞭金藻的Y与CCOC呈多项式相关;胶体对扁藻生长的刺激作用大于球等鞭金藻和海水小球藻。粒径为10kDa—0.2μm的胶体对3种藻的促进作用大于1—10kDa的胶体。河口、河流和生源胶体使小球藻的生长率提高1.5%—14.1%,不同来源的3种胶体对海水小球藻的促进作用依序是河流胶体>生源胶体>河口胶体。以上结果表明,天然胶体含有微藻生长所必需的营养元素,对微藻的生长有显著的刺激作用。Natural colloids are originated from multiple sources from river runoffs, sediment re-suspension, and marine biological processes. Colloids are contained in organic matters in different sizes and concentrations, which would impact phytoplankton growth in different manners. To study the mechanism that would help mariculture and marine pharmaceutical application, we conducted this research on the effect in terms of colloidal organic carbon(COC)concentration on the phytoplankton growth. Water samples were collected from waters near Xiamen(Amoy)of Fujian representing natural river, estuary, and sea water masses, from which natural colloids were abstracted with cross-flow ultrafiltration technique. Phytoplankton samples were algae including Chlorella spp., Platymonas subcordiformis and [WTBX]Isochrysis galbana. Biogenic colloid was isolated from the cultured solution of Chlorella spp.With the cross-flow ultrafiltration technique, the water samples were passed through a 0.2μm pore-size cartridge filter to remove suspended particulate matter, and then ultrafiltrate using a cross-flow ultrafiltration cartridge with a 10kDa or 1kDa hollow-fiber membrane. At the end of ultrafiltration, the colloids in retentate were in sizes of 10kDa? C0.2μm or 1—10kDa.The results showed that the growth rates([WTBX]k)of three algae increased respectively 4.0%—7.0%, 19.1%—28.9% and 2.9%—8.7% at COC concentrations of 4.8—238.4μmol/L and colloidal sizes in 10kDa? C0.2μm. The percentage of relative increase of growth rate([WTBX]Y)for Chlorella spp. was directly related to COC concentration, and the relations between [WTBX]Y and COC concentration for [WTBX]P.subcordiformis and [WTBX]I. galbana were polynomial. The stimulation of marine colloids on [WTBX]P.subcordiformis is greater than [WTBX]I. galbana and the [WTBX]Chlorella spp. Results also showed that the growth rates([WTBX]k)of three algae in the colloids in sizes of 1—10kDa increased respectively by 11.6%, 25.4% and 3.2%, and in the colloids sized in 10kDa? C0.2μm, by 22.5%, 29.5% and 4.5%. The values of [WTBX]k for the three algae in the presence of colloids in sizes of 10kDa? C0.2μm were 1.9, 1.2 and 1.4 times greater than that in sizes of 1—10kDa. This means that bioactivity of high molecular weight marine colloids are higher than those in low molecular weight. The adding of estuarine, riverine and biogenic colloids caused 1.5%, 14.1% and 9.7% enhancement in growth rate of [WTBX]Chlorella spp. respectively. The maximum growth stimulations of Chlorella spp. by the three colloids was riverine colloids, biogenic colloids and estuarine colloids in descending order. It is clear that natural colloids contain nutrients required in growth of phytoplankton, and they can markedly boost the algae growth and be uptaken by algae.国家教育部高等学校骨干教师计划基金资助项目,2005;; 国家自然科学基金重大研究计划重点项目,90411016

型钢混凝土柱粘结滑移性能及ANSYS数值模拟方法研究

进行了20个型钢混凝土构件的推出试验、20根型钢混凝土偏心受压柱试验,以及23根型钢高强混凝土柱在反复荷载作用下的试验。通过在型钢与混凝土之间埋设的电子滑移传感器和电阻应变片,得到了粘结应力和内部滑移沿型钢埋置长度的分布规律;考虑了混凝土强度、型钢的保护层厚度和埋置长度,以及横向配箍率等诸因素,建立了型钢混凝土粘结强度的计算公式;分析了构件长细比、荷载偏心距对粘结强度和滑移应变的影响,提出了偏心受压柱粘结滑移本构关系的一般表达式;研究了型钢高强混凝土柱在动力作用下粘结滑移的分布规律和粘结退化机理,提出了反复加载条件下的粘结滑移本构模型。结合目前广泛应用的大型商业软件ANSYS程序,对型钢混凝土构件的非线性有限元分析方法进行了研究,全面介绍了数值计算中所涉及到的材料模型定义、几何模型建立、单元划分、约束条件处理等技术问题,并提出了采用非线性弹簧单元（Combination39单元）对型钢混凝土粘结滑移进行模拟的实用方法

Radium-226 as a tracer for the source and movement of water masses in the Prydz Bay of Antarctica

中国第22次南极科学考察航次(2005年12月至2006年1月)期间,利用Mn-纤维富集大体积表层海水中的rA同位素,并通过222rn直接射气法测量226rA的比活度,结果表明,普里兹湾表层水的226rA比活度变化为0.92--2.09 bQ/M3,平均值为1.61 bQ/M3,在深海区域表现出226rA含量高的特征。从空间分布看,表层水226rA比活度呈现出由湾内向湾外增加的反常态势,反映出不同rA含量水团的混合影响2。26rA比活度与盐度的关系证实研究海域表层水的rA含量主要受三种端元水体的混合所控制:其一为具有镭含量高、盐度高特征的南极夏季表层水;其二为低镭、低盐特征的冰融水;其三为高盐、中等镭含量的普里兹湾中深层水,该水体的226rA纯粹由海底沉积物间隙水向上扩散所维持。结合S-226rA示踪体系及上述三端元混合模型,计算出各组成水体的比例并描绘出它们的空间分布。南极夏季表层水的份额由湾外向湾内逐渐降低,并且在68°E断面向南影响范围较大;冰融水的比例由湾内向湾外降低,并且在湾内东部的高温水体中具有最大贡献;普里兹湾中深层水中受沉积物镭来源影响比较显著的区域出现在湾内西北部,其影响向湾外逐渐降低。冰融水与普里兹湾中深层水份额分别于普里兹湾湾顶东、西部出现高值的分布特征证实埃默里冰架前沿海流东进、西出的运移规律。Surface waters were collected from the Prydz Bay of Antarctica for measurements of 226Ra activities during the 22nd China's antarctic research expedition(from December 2005 to January 2006).226Ra in large volume waters was extracted by Mn-fibers and measured by a 222Rn emanation method.Results show that 226Ra activity in the Prydz Bay ranges from 0.92 to 2.09 Bq/m3 with an average of 1.61 Bq/m3.High contents of 226Ra were observed at deep open-ocean stations,which induced an offshore increase of 226Ra activity.This unique distribution pattern in the Prydz Bay was ascribed to the influence of water masses with different 226Ra signals.Based on the relationship between 226Ra activity and salinity,three water masses were identified:(1) the antarctic summer surface water(AASSW) with a characteristics of high salinity and high content of 226Ra;(2) the ice-melted water(IMW) with a characteristics of low salinity and low content of 226Ra;and(3) the Prydz Bay deep water(PDW) with a characteristics of high salinity and intermediate content of 226Ra.226Ra in the Prydz Bay deep water was assumed to be maintained only by the supply from the seabed sediments.The fractions of the above three water masses were estimated by mass balance calculation of salinity and 226Ra activity.Results show that the AASSW fractions decrease from the deep open ocean to inshore,while the IMW and PDW fractions increase.The maximum fractions of the IMW and the PDW occur in the eastern and the western regions along the front of the Amery ice shelf,respectively,testifying the major flow into the shelf occurs at the eastern end and exits from the west.国际极地年中国行动计划项目;国际海底区域研究开发“十一五”项目(DYXM-115-02-4-06);福建省自然科学基金杰出青年基金项目(2009J06026