Application of Ionic Liquids in Extraction and Separation

室温离子液体是由正负离子组成的室温为液体的熔融盐,因其具有极低的蒸汽压、可设计性和特殊的选择溶解能力等独特的性质,使得其在萃取分离有机物及金属离子、液相微萃取和汽油柴油中脱硫及碱性氮化物等领域都有着广泛应用。综述了离子液体在萃取分离上的应用进展。Ionic substances with melting points close to room temperature were referred as room-temperature ionic liquids,which showed lots of unique properties,e.g.environmentally benign,nonvolatile,designable and were good solvents for a wide range of both organic and inorganic materials.Ionic liquids are widely used in extraction and separation processes,e.g.extraction and separation of metal ions and organic compounds,headspace liquid-phase micro extraction,removal of basic nitrogen compound from distilled diesel and desulphurization of gasoline.In this paper,the recent application of ionic liquids in extraction and separation were reviewed.教育部科学技术研究基金重点资助项目(207108

Autophagy Inhibition by Chloroquine Sensitizes Cervical Cancer SiHa Cells to CPT Treatment

自噬诱导是肿瘤细胞对化疗药物抵抗性的原因之一,该研究探讨溶酶体抑制剂氯喹对喜树碱(camptothecin,CPT)诱导的宫颈癌细胞Si Ha死亡的增敏效果。CPT和/或氯喹处理宫颈癌Si Ha细胞,MTT法检测细胞增殖,DAPI和TUNEL染色观察细胞凋亡,Western blot和免疫荧光检测自噬及凋亡相关蛋白。结果发现,CPT处理后,Si Ha细胞MAP1LC3B荧光点和LC3II(microtubuleassociated protein light chain 3II)蛋白水平增加,p62荧光点和蛋白质水平则减少;而采用氯喹特异抑制自噬后,可明显提高CPT诱导的细胞凋亡、caspase-9的激活和PARP(poly ADP-ribose polymerase)的切割,而全长caspase-2水平显著下降。以上结果提示,氯喹可通过抑制细胞自噬而增强宫颈癌细胞株Si Ha对CPT诱导细胞凋亡的敏感性。The autophagy induction is one of the reasons for the resistance of tumor cells to chemotherapy drugs. In this study, the enhanced sensitivity of cervical cancer Si Ha cells to camptothecin(CPT)-induced cell death by chloroquine(a lysosome inhibitor) was investigated. The cell viability was detected by MTT assay, meanwhile, apoptosis was observed by DAPI and TUNEL, autophagy related proteins and apoptosis proteins were analyzed by immunofluorescence(IF) staining and Western blot in Si Ha cells after CPT treatment alone or combined with chloroquine. The results found that in Si Ha cells with CPT treatment the autophagy related protein LC3 foci and microtubule-associated protein light chain 3II(LC3II) protein level was increased, but p62 foci and protein level was decreased. When autophagy was inhibited by chloroquine, the CPT-induced apoptosis was obviously enhanced, and caspase-9 was activated and PARP was cleaved, but full length caspase-2 was decreased. Taken together, these results indicated that the inhibition of autophagy by chloroquine could sensitize cervical cancer Si Ha cells to CPT inducing cell apoptosis.山西省自然科学基金(批准号:2014021037-9);; 山西医科大学汾阳学院博士启动基金(批准号:1301);山西医科大学汾阳学院科研项目基金(批准号:1422)资助的课题~

福建省实验动物学学科发展研究报告

1实验动物科学概述1.1实验动物科学的概念实验动物科学(lAbOrATOry AnIMAl SCIEnCES)是以实验动物资源研究、质量控制和利用实验动物进行科学实验的一门综合性学科。在《学科分类与代码标准》(gb/T13745-1992)中,与实验动物科学有关的学科分别是“实验动物学“、“医学实验动物学“、“比较病理学“等

庚型肝炎病毒基因在大肠杆菌中表达的初步研究

利用原核表达载体 pRSET或 (和 )pGEX在大肠杆菌内表达了覆盖庚型肝炎病毒 (HGV)C NS3和NS5区的多段基因。CE1、E2、NS3、NS5及NS3 NS5嵌合基因等的 8段基因均有高效表达 ,各重组蛋白产量与菌体总蛋白之比在 10 %～ 35 %之间。对以上重组蛋白进行免疫学筛选 ,证实其中 7个重组蛋白均具免疫学活性 ,在一定程度上确定了重组HGV抗原表位的分布 ,为HGV的血清学和免疫学诊断试剂的研究奠定了坚实基

DDEFL1的结构和功能研究进展

DDEFL1又称为ASAP3(、ACAP4或UPLC1,属于小G蛋白家族中Arfs家族的一种ArfGAP。人的肝和肺是DDEFL1表达水平较高的两个组织[1]。了解DDEFL1的结构及功能,可以为肝癌或其它癌症患者提供一种新的基因治疗方案

巢湖铁线莲(毛茛科)原白中一个排印错误的改正

在规范化整理保存于中国科学院植物研究所国家植物标本馆(PE)的模式标本时,发现巢湖铁线莲(毛茛科)原白中错误地将主模式标本引证为王德群70543,但实际为王德群95043,特予以更正

The Trade Relationships between the Fujian Businessmen and the Nguyen Regime of Nam Ha in Vietnam & the Spread of Fujian's Culture

在1600—1774年南河阮氏政权存续期间,闽商与南河的贸易得到了持续不断的发展。该发展过程大致可分为1600—1639贸易爆发期、1640—1683郑氏垄断贸易期和1684—1774全面发展3个阶段。贸易的发展促进了闽商与南河社会的交流,带动了众多的闽人移居南河。相应地,福建的物质文化、精神文化和制度文化得以在广南传播、发展与融合,为南河经济文化的发展和闽越经济文化交流作出了重要贡献。When the Nguyen Regime of Nam Ha in Vietnam last in 1600-1774,the Fujian Businessmen has been engaged in trade with the Nguyen Regime of Nam Ha for always.The trade relationships can be roughly divided into three stages:the outbreak period in 1600-1639,the Zheng's Monopoly period in 1640-1683 and all-round development period in1684-1774.As a result,this leads to the further social communication and the emigration of Fujian to the Nam Ha with the trade development.Accordingly,the cultural of Fujian,which is material,spiritual and institutional,could be availably broadcasted,promoted and fused together in Guangnan.This has made the important contributions to economic and cultural exchange with Fujian and Vietnam

Agouti and Agouti-related Proteins

Agouti有多方面的意义。首先,它是分属几种不同物种的哺乳动物的俗称。其次,它是一些啮齿类动物的属名。第三,它代表了一种啮齿类动物特有的毛色。第四,它是一个与黑素皮质素受体信号转导途径的调控有关,存在于从人(Homo sapiens)到啮齿类动物,乃至斑马鱼(Danio rerio)的一个特定基因的名称,又是该基因产生的一种特定信号蛋白的名称。Agouti和Agouti相关蛋白是肿瘤、肥胖与糖尿病等人类疾病的研究热点之一。Agouti黄色肥胖小鼠最近成了表观遗传学研究的一个重要模型动物。本文阐述并区分了与agouti有关的一些重要概念与科学术语,并综述了相关领域的研究进展。Agouti and Agouti-related proteins are important signal proteins involved in the regulation of the melanocortin receptor signaling.Aberrant or ectopic expression of these proteins has been shown to cause abnormal coat color pigmentation and development of obesity,diabetes and tumors.A number of mouse models carrying mutations for agouti or agouti-related proteins are being utilized to study the underlying pathogenic mechanisms for a number of important human diseases.This review attempts to summarize a few aspects of important research progress in this area.厦门大学引进人才科研启动费(厦大0000X071E9

Expression and significance of aldose reductase in tissues and its enlightenment for nursing research

醛糖还原酶(AldOSE rEduCTASE,Ar)属还原型辅酶Ⅱ依赖的醛-酮还原酶家族,定位于细胞质,是机体葡萄糖代谢过程中多元醇通路的第一个作用酶和限速酶,在哺乳动物各组织器官中广泛分布,在人类的肾髓质中表达最高。Ar的异常激活和高表达在糖尿病(dIAbETES MEllITuS,dM)及并发症的发生发展中国家973项目;编号:2009CB9416010; 国家自然科学基金项目;编号:30970649、31271239; 福建省科研基金项目;编号:2010L0002; 厦门大学细胞应激生物学国家重点实验室开放课题基金; 细胞生物学与肿瘤细胞工程教育部重点实验室访问学者基金资