不同波段可见光对雨生红球藻生长与虾青素积累的影响

厦门市科技项目(3052Z20031086); 国家大学生创新性实验计划(2007); 福建省大学生创新性实验计划(2007); 厦门大学大学生创新性实验计划(2007); 新加坡厦门大学校友基金会项目(2007)资

野外地表渗透系数测量装置与方法研究

野外地表的渗透系数是小流域降雨产流和边坡稳定性计算分析必不可少的参数。介绍了新的野外地表渗透系数的测量装置与方法。该装置结合了降雨入渗试验和双环入渗试验装置的优点,实现了斜坡地表的饱和无压入渗测量。试验数据分析方法可以直接获得地表土层的渗透系数。在某山区流域进行了入渗试验并分析获得了土层的渗透系数与吸力

INITIAL STUDIES ON THE EFFECTS OF STRESS CONDITIONS ON ASTAXANTHIN ACCUMULATION OF HAEMATOCOCCUS PLUVIALIS

通讯作者: 蔡明刚, E-mail:[email protected] 作者简介: 黄水英(1981-),女,硕士研究生,目前主要从事微藻天然产物化学的研究.[中文文摘]虾青素(Astaxanthin)是一种近年来进入国际研发领域的红色类胡萝卜素,广泛存在于自然界,尤其是海洋生物体内,其抗氧化能力比其他类胡萝卜素强10倍,比维生素E强550倍,被誉为"超级维生素E"。虾青素的着色能力显著,并具有抗癌症、抗衰老、增强免疫力等一系列重要的生理功能,鉴于此,它被广泛应用于保健品、医药、水产养殖等领域,具有广阔的发展前景。在虾青素的生物来源中,雨生红球藻(Haematococcuspluvialis)中虾青素可占其干重的1%～3%,且体内虾青素及其酯类的结构配比与养殖对象所需一致,因此被公认为自然界中虾青素的最佳来源(蔡明刚等,2003)。[英文文摘]Induction of astaxanthin accumulation in Haematococcus pluvialis by three stress conditions was studied,which including nitrogen deficiencies,salt stress and illumination with different wavelengths.Nitrogen deficiencies(NaNO_3≤0.012 g/L)were not favorable to the growth of the H.pluvialis,but they could promote the astaxanthin accumulation in the planospore and reduce the ratio of aplanospore to no more than 15%.High salinity could induce the production of astaxanthin in H.pluvialis in a short time,with the highest levels of astaxanthin under the NaCI eoneentar tion of 2.5 g/L However,such increases in salinity were accompanied by high rates of cell mortality.The illumination with diferent wavelengths has quite diferent effects on the growth and astaxanthin accumulation of .H pluvialis:red light was favorable to the growth of the alga,while blue light enhaneed the astaxanthin porduetion.Th effets of the illumination with different wavelengths enhaneed in the following odrer:blue light,red light,green light and white light.厦门市重点科技项目(3052Z20031086);新加坡厦门大学校友基金会项目(2007);国家大学生创新性实验计划(2007);厦门大学大学生创新性实验计划(2007)等资

Molecular Cloning and Its Transformation of ACC Synthase NtACS1Gene from Narcissus tazetta var.Yunxiang'

为探究多花水仙ACS基因的序列特征及功能,以云香'水仙盛花期花瓣为试验材料,根据云香'水仙花朵转录组数据信息,通过RT-PCR方法克隆出1个AC; S基因,命名为NtACS1(GenBank; KX082936);NtACS1开放阅读框(ORF)长度为552bp,编码183个氨基酸。编码蛋白质分子量约为20.6KDa,理论等电点为6.3; 0,不稳定系数为65.49,属于不稳定的疏水性蛋白。通过qRT-PCR对云香'水仙不同时期花瓣和副冠中的NtACS1基因进行了表达分析,得到与云; 香'水仙花朵转录组数据中相同的结果:NtACS1基因在云香'水仙花瓣和副冠中的表达都是随着花衰老过程呈现逐渐下降的趋势,且NtACS1基因在花瓣; 和副冠中的表达峰值都在花苞期,表明; NtACS1基因编码的蛋白是在乙烯生物合成途径的系统1发挥催化作用的ACC合成酶。成功构建了NtACS1基因的正义植物表达载体,并通过农杆菌介导; 法获得8株转基因烟草,PCR和RT-PCR检测显示其中有6株为阳性植株,初步证实NtACS1基因已导入烟草基因组中且在烟草中已表达。该研究结果为; 进一步分析NtACS1基因的功能和后续转化水仙延长其花期研究奠定了基础。Aimed to study the characteristics and functions of ACSgene,in the; present study,we cloned a 1-aminocyclopropane-1-carboxylate synthetase; gene named NtACS1(GeneBank KX082936)based on the RNA-Seq database from; the flower of Narcissus tazetta var.Yunxiang'using RT-PCR method.The; length of the open reading frame(ORF)of ACSis 552bp,encoding 183amino; acids coupled with a molecular weight of 20.6kDa and theoretical; isoelectric point of 6.30.qRT-PCR analysis showed that the relative; expression level of NtACS1both in petals and coronas are decreased; gradually along with the aging of flower.Moreover,the expression data of; NtACS1gene were consistent with those obtained by RNA-Seq, implied that; the NtACS1protein as an ACC synthetase might play a role in the; catalytic system 1 of ethylene biosynthesis.Furthermore,sense plant; expression vectors of NtACS1were successfully constructed with; agrobacterium mediated transformation,and 6positive transgenic tobacco; plants were ultimately obtained. Our current study will lay an; experimental foundation for the future application of the genetic; transformation to prolong florescence ofYunxiang'.福建省种业创新与产业化工程项

Isolation and Function Analysis of NtWRKYY1Transcription Factor Gene in Narcissus tazetea var.Yunxiang

为明确WRKY转录因子在云香水仙中的功能,该研究以云香水仙为材料,克隆了WRKY基因,命名为NtWRKYY1(GenBank登录号为KX0564; 95)。序列分析显示,NtWRKYY1基因开放阅读框(ORF)长度为510; bp,编码169个氨基酸。多序列比对和系统进化树分析显示,NtWRKYY1编码蛋白含1个WRKY结构域和C_2H_2锌指结构(Cx_4Cx_(2; 3)HxH),与AtWRKY57聚在一起,属于第Ⅱc类WRKY转录因子。组织表达和时空表达分析显示,NtWRKYY1基因在花中表达量高于根和叶,; 且在花瓣及副冠中的表达量随开花过程(花蕾期、始花期、盛花期、衰败期)呈上升趋势。植物激素和非生物胁迫分析显示,NtWRKYY1基因受脱落酸(AB; A)、高温、干旱和盐诱导,受茉莉酸甲酯(JA)抑制,表明NtWRKYY1基因可能在云香水仙花朵的衰老过程中起正调节作用,同时参与云香水仙ABA、; JA等激素信号转导及高温、干旱、盐碱等非生物胁迫过程的调控。利用In-Fusion克隆技术成功构建过表达载体pMDC140-NtWRKYY1,并; 采用农杆菌介导叶盘法转化烟草。RT-PCR和GUS染色结果显示,目的基因已成功导入烟草基因组中。WRKY transcription factors play an important adjusting role in the; process of plant growth and development,hormone signal transduction and; abiotic stress response.To clarify the gene Narcissus tazetea; var.Yunxiang,we cloned the NtWRKYY1gene(GenBank accession; No.KX056495)based on Yunxiang,analyzed the gene sequence; features,evolutionary relationship and expression characteristics,; constructed the expression vector before transformed into; tobacco.Sequence analysis revealed that the length of NtWRKYY1gene open; reading frame(ORF)is 510bp,encoding apolypeptide of 169amino; acids.Multiple alignments and phylogenetic analysis showed that; NtWRKYY1protein containing one WRKY consecutive structural domain and; C_2H_2type zinc finger(Cx_4Cx_(23)HxH)belong toⅡc sub-group of WRKY; transcription factor together with Arabidopsis AtWRKY57.Tissue-specific; expression and temporal and spatial expression showed that; NtWRKYY1inYunxianghad a much higher expression in flowers than that in; roots and leaves,and a rising express trend in petal and corona of; alabastrum stage,early flowering stage,full bloom stage and faded; stage.NtWRKYY1can be induced by abscisic acid(ABA), high; temperature,drought and saline,and restrained by methyl; jasmonate(JA)through hormones and abiotic stresses analyzing.We can; concluded that NtWRKYY1gene may play a regulating role during the flower; senescence process inYunxiang,involve in the hormone signal transduction; of ABA,JA and the abiotic stress regulation of high temperature,drought; and saline at the same time.In addition,we constructed overexpressing; vector pMDC140-NtWRKYY1using In-Fusion cloning technique,transformed; into tobacco by the method of Agrobacteriumthough leaf disc; transformation.The carrier of PCR and GUS staining results indicated the; resistant plantlets were positive.This study will make a good foundation; for further exploring the function of the WRKY transcription factor; inYunxiang.福建省种业创新与产业化工程项

基于物联网的智慧家庭健康医疗系统

近年来,人们越来越重视自身以及家人的身体健康状况,针对这种现状研发了智慧家庭健康医疗系统。采用血压计、体脂秤和血糖仪等医疗设备定期获取血压、体脂和血糖等生理数据,再通过网关转发给服务器,然后通过大数据处理分析把异常情况反馈给用户,用户可以登录健康云平台实时了解自己的健康状况,及时采取相应的措施。此外,还提出了基于多传感器信息对老人跌倒算法进行判决,实现对老人跌倒的实时报警。大量测试与分析表明,该系统运行稳定可靠,对未来针对智慧医疗方面更多的应用研究具有一定的指导意义

具有循环净化性能的纳米TiO_2/活性炭复合表面的构筑

目的构筑净化性能优异的纳米TiO_2/活性炭复合表面。方法采用溶凝胶模板法构筑球形表面,将乳化法制备的石蜡球和复合活性炭的纳米TiO_2溶胶挤压成形,温和处理后形成规则稳定的球形表面。使用SEM、XRD和FTIR进行表面微观结构、形貌和物相检测,采用X射线断层扫描对球形表面结构进行重构和球面特征分析。分析测定了不同当量直径球形表面的样品对亚甲基蓝的净化效果。结果温和处理工艺维持了原始P25粉末的晶型和晶粒尺寸,溶凝胶模板法可以形成贯通、多面、多孔径的结构和簇状均匀表面。3DCT结果显示,活性炭的复合使样品形成了碳增强的TiO_2球形表面。构筑成功的纳米TiO_2复合活性炭样品,在8个周期的循环吸附-净化中可以完全将亚甲基蓝净化降解。平均当量直径为0.67mm的样品,在9个周期的净化反应中,净化率可以保持100%。结论溶凝胶模板法成功构筑了纳米TiO_2复合活性炭的球形连续表面,球形表面之间大的孔道连接和活性炭在纳米TiO_2颗粒之间的复合,可以提高传质效率,从而提高循环净化性能

Large Culures for Haematococcus pluvialis Outside and Accumulation of Astaxanthin

针对雨生红球藻(Haematococcus pluvialis)大体积室外培养开展实验,对不同品系藻株、不同营养方式、胁迫条件等对雨生红球藻生长及虾青素积累的进行了初步研究.结果表明,HPM品系在室外培养过程中指数生长率为0.22,胁迫后虾青素产率为1.35mg/L,均优于其他两个品系.室外培养中加入醋酸钠的混合培养基可以缩短胁迫时间,并且其虾青素的积累量比光合自养培养下提高了126.3%.盐胁迫比高光胁迫更能有效地积累虾青素,虾青素产率从0.76 mg/L提高到了1.33 mg/L.The green microalga Haematococcus pluvialis was cultured outside in large volume,the effects of different trophic and stress conditions on cell growth and astaxanthin accumulation were studied.The effects of different strains on accumulating astaxanthin was also investigated.The results showed that the growth rate of the strain HPM outside was 0.22,and the astaxanthin production was 1.35 mg/L,which were higher than the other two strains.The stress period was shortened under the mixotrophic system with addition of NaAc,and the astaxanthin content moreased 126.3% than that under the autotrophic system.The stress of high salinity was better to accumulate astaxanthin than high light intensity.Under the stress of high salinity,the astaxanthin production was 1.33 mg/L compared to the data under the stress of high light(0.76 mg/L).厦门市科技项目(3052Z20031086)资

Effect of nitrogen addition on seed germination competition of Phragmites australis and Suaeda salsa under salt stress

为了探讨黄河三角洲盐地碱蓬和芦苇种子萌发阶段在不同盐胁迫条件的竞争关系,以及氮添加对其竞争的影响,本实验采用培养皿纸上发芽床法,分析了不同浓度氮、盐及混合培养等条件下两种种子发芽率、发芽速度的响应。结果表明,盐胁迫对盐地碱蓬和芦苇种子发芽率均有显著性影响,盐浓度低于300 mmol·L^-1时盐地碱蓬发芽率并未有显著降低,而芦苇种子发芽率随着盐浓度增加而呈现显著降低趋势;低盐条件下,氮添加未对两物种发芽率产生显著影响,但高盐胁迫条件下适度的氮添加显著增加了种子发芽率。混合培养显著增加了低盐环境中芦苇的发芽率,当盐胁迫浓度升高时,培养模式对两物种发芽率影响程度降低。对于发芽速度而言,低盐处理下单独培养盐地碱蓬的发芽速度显著高于混合培养,氮添加对盐地碱蓬发芽速度未产生显著影响;而低盐条件(0、100 mmol·L^-1)下芦苇的发芽速度则是在混合培养高于单独培养方式,当盐浓度升高(400 mmol·L^-1)时,混合培养抑制了芦苇的发芽速度,此时,高氮(40 mmol·L^-1)添加处理则能够显著缓解混合培养对芦苇发芽的抑制作用。研究结果可以为黄河三角洲滨海典型物种生态适应机制研究提供一定的理论依据

油页岩热解中间产物的反应特性

对油页岩在不同终温热解所得半焦进行溶剂萃取,得到了热解中间产物(热解沥青)和终产物.采用气相色谱-质谱联用仪分析了热解沥青和页岩油的组成,采用热裂解-气相色谱-质谱联用仪检测了热解沥青的裂解产物,并与热解页岩油进行对比,研究了油页岩热解沥青的反应特性.结果表明,油页岩热解沥青在最快产油阶段生成最多且稳定存在,375℃时收率最大;375~400℃时热解产油速率最大,热解沥青收率下降,表明热解沥青在高温下不稳定,有机质生成中间产物后便转化成终产物.随热解温度升高,热解沥青中轻质组分含量增加.杂原子化合物最先从有机质上断裂进入中间产物,而热解油中烯烃化合物大部分来自有机质直接裂解,来自热解沥青裂解的较少.热解温度升高,热解沥青的裂解产物中烯烃含量增加,且热解沥青中芳香烃的长侧链易通过裂解变短.</p