Method of Edge Optimizing Based on Self-organizing Directing

传统的边缘检测方法通常采用预先的平滑滤波操作排除噪声的干扰 ,这使得去除噪声的同时也损失了图像的细节 .因此 ,滤波去噪比例和边缘检测精度一直是一对矛盾 .本文针对这个问题提出了一种基于边缘定向的边缘优化策略 .边缘具有一定的方向是它区别于杂乱无序的噪声的鲜明特征 ,利用这一特征 ,可以不需要平滑滤波操作并能很好地解决以上问题 .文中还根据数字图像的特点提出了自组织定向这一获取图像边缘方向的有效方法 .The method of traditional edge detecting often adopts pre-filtering to eliminate the disturbing of the noise, which makes the details loss while getting rid of the noise. So, the proportion of filtering and the precision of edge detecting are a pair of contradictions all along. This paper presents a strategy of edge optimizing based on edge directing aiming at the question. It's a distinct difference compared with the noise that the edge has some direction, which needn't pre-filtering anymore and can make that problem solved primly. Meanwhile, we present a method of self-organizing directing which can obtain the direction of the edge effectively

金融服务、市场建设与预防腐败的基础理论和关系研究

当前,金融全球化浪潮正席卷世界,由美国引发的金融危机,对我国经济的影响仍在继续蔓延和深化,金融行业的反腐倡廉工作将比以往任何时期更为重要。为此,由上海金融学院和上海市人民政府发展研究中心主办、上海市金融纪律检查工作委员会协办、上海金融学院国际金融研究院和科研处承办的“第六届上海国际金融中心建设论坛“,于2012年3月31日在上海金融学院举行。本次论坛以“金融反腐倡廉“为主题,对金融服务、市场建设与预防腐败的基础理论和相互关系进行了深入研究,剖析了经济转型过程中金融腐败的诱因、机会和产生机理。本刊选登部分内容,以飨读者

论深化公共服务供给侧结构性改革

深化公共服务供给侧结构性改革,提升人民群众的公共服务获得感、公平感和幸福感,是坚持以人民为中心的发展思想的应有之义。从国家、社会和个人层面看,深化公共服务供给侧结构性改革,是推进国家治理体系和治理能力现代化的需要,是打造共建共治共享社会治理格局的需要,也是满足人民对美好生活的向往的需要。当前,公共服务供给侧存在的结构性问题是:公共服务供给不能适应公共服务需求变化造成供给总量不足、分布不均、质量低下,主要表现在供给主体失衡、供需匹配失衡、供给城乡失衡以及供给人群失衡等方面。深化公共服务供给侧结构性改革的本质是权利和利益的再分配,必须理顺政府、市场和社会的关系,精准识别需求匹配供需关系,统筹城乡发展,深化户籍制度改革,实现公共服务供给一体化和均等化。国家社科基金项目“基于国家经济治理政策变迁的供给侧结构性改革研究”(16BJL078)成

Establishment of a nude mouse model of glioma orthotopic xenograft with double-fluorescent labeling

目的建立一种稳定、可实时监测的胶质瘤原位移植瘤裸鼠模型。方法用带有荧光素酶（luciferase-Luc）和绿色荧光蛋白（green fluorescent protein-GFP）基因的慢病毒感染U251神经胶质瘤细胞,流式细胞仪筛选稳定表达GFP-Luc荧光的细胞系,并通过CCK-8实验、细胞周期实验、Transwell肿瘤迁移及侵袭实验等评价荧光细胞的增殖、迁移和侵袭能力是否改变;将细胞接种至裸鼠大脑尾状核,建立胶质瘤原位移植瘤模型,利用小鼠活体成像系统监测脑内肿瘤的生长情况,并通过石蜡切片,HE染色评价细胞在裸鼠脑内的病理特征及成瘤能力。结果成功构建稳定表达GFP荧光和luciferase荧光的U251胶质瘤细胞系及动物模型,慢病毒整合并未改变细胞的增殖、迁移及侵袭能力;模型生长周期适中,成瘤率高,瘤体在颅内生长稳定,HE切片符合人胶质瘤特征。结论双荧光标记的胶质瘤细胞相比于传统细胞更有利于胶质瘤动物模型的实验研究;U251-GFP-Luc胶质瘤细胞裸鼠模型,其肿瘤生长和病理特性与人胶质瘤相似,且可实时观察肿瘤生长,可作为胶质瘤实验研究的理想动物模型。Objective To establish a stable and real-time monitorable nude mouse model of orthotopic glioma xenograft. Methods U251 glioma cell line was infected by a leutiviral vector containing green fluorescent protein （GFP） and luciferase （Luc） gene. Ceils stably expressing fluorescence of GFP and Luc were sorted by flow cytometry. CCK-8 test and Transwell tumor invasion and migration assay were used to compare the biological features between the ceils stably expressing GFP-Luc fluorescence and cells without fluorescence. Then the ceils were implanted intracranially in the right caudate nucleus of athymic BaIb/c nude mice to establish the tumor model. The growth of intracerebral tumor was monitored over time by a bioluminescence imaging （BLI） system. Hematoxylin-eosin （HE） staining was used to evaluatethe histopathological features and tumorigenicity of the transplanted glioma cells in the brain of nude mice. Results U251 glioma cell line with stably expressing GFP-Luc fluorescence and the corresponding orthotopic xenograft model were successfully established. There was no statistically significant difference in the proliferation, invasion and migration abilities between the cells with stably expressing GFP-Luc fluorescence and the control cells. This model showed a high tumor formation rate and stable tumor growth, and takes a moderate time to establish this model. Conclusions Compared with the traditional glioma cells, GFP-Luc-transfected human glioma cells are more feasible for the studies of glioma in vivo. The tumor growth and pathological characteristics in this U251-GFP-Luc glioma model are similar to human glioma, and the growth of this tumor can be real-time monitored. It can be used as an ideal animal model for experimental studies of glioma.国家自然科学基金（编号：30970733）

镓在抗菌方面的研究进展

抗生素发现\"黄金时代\"的结束以及日趋严重的抗生素耐药性问题给我们留下一个戏剧性的、缺乏新型药物来防治耐药病原体感染的难题。这使得从现有非抗生素药物中筛查抗菌活性药物成为一种新尝试。近期研究表明:美国食品药品监督管理局(FDA)批准的、本来用于治疗高血钙的药物硝酸镓具有良好的抗菌活性。不同于其他抗生素,镓的药理性质依靠化学拟态,它可以在目标酶分子中取代铁,从而干扰、扰乱细菌含铁蛋白的功能及细菌代谢,无论是在体外实验还是在体内实验中,镓对多种病原体,包括多重耐药病原体都表现出杀菌活性。因此一些含镓制剂有望被开发成有潜力的非传统抗菌药物,帮助治疗多重耐药细菌感染。国家自然科学基金(No.81673242、No.81473251、No.31741006、No.31370166和No.81301474);;福建省自然科学基金(No.2014J01139和No.2015J01345);;厦门大学校长基金(No.20720160060

基于栖息地风险评价模型的海岸带滩涂湿地风险评价——以闽三角为例

海岸带湿地是生物多样性最丰富、生产力最高、最具价值的生态系统之一。然而近年来,随着城市化和工业化进程的加快,我国海岸带地区的土地利用正发生深刻变化,生态风险凸显。利用2000、2005、2010和2015年的Landsat TM/ETM+和Landsat 8 OLI遥感影像,融合行政区划图与地形图,并引入一个评价多元海洋、海岸带利用对栖息地造成风险的模型,即Integrated Valuation of Ecosystem Services and Trade-offs(In VEST)模型中的栖息地风险评价(Habitat Risk Assessment,HRA),模型评价闽三角海岸带滩涂湿地的生态风险。结果表明:(1)闽三角海岸带滩涂湿地风险以低风险为主;(2)泉州地区的风险面积最大(约4389.91 hm~2),漳州地区最小(约4630.73 hm~2);(3)修正面积影响的情况下,其他建设用地造成的暴露和影响较大,耕地造成的暴露和影响最小。不同区域滩涂湿地风险程度的可视化表达,可以揭示滩涂湿地高风险地区以及高风险的产生原因,便于管理者对滩涂湿地采取精准的保育措施。国家重点研发计划(2016YFC0502900);;福建省科学技术厅民生科技专项(引导性项目)(2017Y6002);;福建省自然科学基金资助项目(2015J01122

遥感-岩石力学(III)——交汇断层粘滑的热红外辐射与声发射规律及其构造地震前兆意义

以构造地震孕震机制之三即交汇断层粘滑发震为模拟对象,利用双轴加载实验系统和红外热像仪对自然界经常出现的断层组合模式——不同交汇角度的组合断层的物理模型进行了室内加载实验,利用红外热像仪和声发射仪分别测定了加载过程中试样表面的红外辐射及断层活动的声发射时间序列。结果表明:(1) 声发射及红外辐射的时间序列特征受加载过程中试样的力学行为所控制,并表现出阶段性变化特点;(2) 声发射与红外辐射特征与断层几何结构有密切关系,当主应力与主断层成45°角时,红外辐射异常区主要沿主断层发育;(3) 垂直交汇的断层与斜交断层的声发射及红外辐射特征有明显差异,交汇角度(1°～90°)越大,交汇点处的红外辐射异常越明显

Wide-Range Tunable Dynamic Property of Carbon Nanotube-Based Fibers

Carbon nanotube (CNT) fiber is formed by assembling millions of individual tubes. The assembly feature provides the fiber with rich interface structures and thus various ways of energy dissipation, as reflected by the non-zero loss tangent (>0.028--0.045) at low vibration frequencies. A fiber containing entangled CNTs possesses higher loss tangents than a fiber spun from aligned CNTs. Liquid densification and polymer infiltration, the two common ways to increase the interfacial friction and thus the fiber's tensile strength and modulus, are found to efficiently reduce the damping coefficient. This is because the sliding tendency between CNT bundles can also be well suppressed by the high packing density and the formation of covalent polymer cross-links within the fiber. The CNT/bismaleimide composite fiber exhibited the smallest loss tangent, nearly as the same as that of carbon fibers. At a higher level of the assembly structure, namely a multi-ply CNT yarn, the inter-fiber friction and sliding tendency obviously influence the yarn's damping performance, and the loss tangent can be tuned within a wide range, as similar to carbon fibers, nylon yarns, or cotton yarns. The wide-range tunable dynamic properties allow new applications ranging from high quality factor materials to dissipative systems

Effects of transfection with acidic fibroblast growth factor by electroporation on skeletal muscle satellite cells

背景:课题组早期研究表明体外一定剂量酸性成纤维细胞生长因子对骨骼肌卫星细胞增殖有促进作用。目的:进一步验证电穿孔转染酸性成纤维细胞生长因子基因对骨骼肌卫星细胞生长、增殖及分化的影响。方法:原代培养、纯化骨骼肌卫星细胞,将带有酸性成纤维细胞生长因子基因的质粒P SECTAg-gfP-A fgf通过电转染的方法转染大鼠骨骼肌卫星细胞,荧光显微镜观察绿色荧光蛋白的表达情况并计算转染率,以流式细胞仪分析转染后细胞周期,绘制细胞生长曲线,观察转染后肌管形成情况,WESTErn blOTIng检测酸性成纤维细胞生长因子基因的表达。结果与结论:1免疫细胞化学检测:骨骼肌肌动蛋白呈阳性表达。2转染效率:P SECTAg-A fgf质粒电转染12 H后即可看见散在发绿色荧光的卫星细胞,72-96 H达高峰,阳性表达率约90%。3细胞周期检测:电转染后S期所占的百分比明显多于未转染对照组(P<0.05)。4细胞生长曲线检测:电转染细胞接种后第3天进入对数生长期,第5天后开始减少。5分化能力观察:电转染组肌管较未转染对照组明显减少,老化细胞较少。6WESTErn-blOT:酸性成纤维细胞生长因子基因在转染骨骼肌卫星细胞中表达。结果表明,通过电穿孔法可以将酸性成纤维细胞生长因子基因转染进骨骼肌卫星细胞并获得高效持久的表达,并有促进骨骼肌卫星细胞增殖及抑制分化为肌管的作用。BACKGROUND: Previous studies have shown that a certain dose of acidic fibroblast growth factor can promote skeletal muscle satellite cell proliferation in vitro.OBJECTIVE: To investigate the effects of transfection with acidic fibroblast growth factor by electroporation on growth, proliferation and differentiation of skeletal muscle satellite cells.METHODS: Skeletal muscle satellite cells were cultured and purified, and then transfected with plasmid p Sectag-GFP-a FGF by electroporation.The expression of green fluorescent protein was observed under fluorescence microscope, and the transfection efficiency was calculated.After transfection, cell cycle was analyzed by flow cytometry to draw the growth curve of skeletal muscle satellite cells.Western blot assay was employed to measure protein level of acidic fibroblast growth factor.RESULTS AND CONCLUSION:(1) Immunocytochemistry detection: The skeletal muscle satellite cells were positive for a-sarcomeric actin.(2) Transfection efficiency: At 12 hours after transfection with p Sectag-a FGF, several cells showed green fluorescence, and the green fluorescent expression reached the peak at 72-96 hours after transfection with a positive rate of about 90%.(3) Cell cycle: After electrotransfection, the proportion of cells at S phase in the electroporation group was higher than that in the control group(P < 0.05).(4) Cell growth curve: At 3 days after electrotransfection, the cells entered logarithmic growth phase but the proliferation slowed down at 5 days.(5) Differentiation capacity: There were fewer myotubes and aging cells in the electroporation group than the control group.(6) Western blot assay: Acidic fibroblast growth factor protein was highly expressed in the cells transfected with target gene detected by western blot assay.These findings indicate that by using electroporation method, acidic fibroblast growth factor can be transferred into skeletal muscle satellite cells and have a high-efficiency and long-term expression, which can promote the proliferation of skeletal muscle satellite cells and inhibit formation of myotubes