不同生物炭材料的制备及其在Li-S电池中的应用（英文）

通过可再生生物质制备的生物炭具有成本低、环保和资源可再生的优势。本研究以分布广泛的稻谷壳、芒草、杉木和柚子皮等生物质为原料,制备了4种不同类型生物炭,然后研究了其作为锂-硫电池硫/碳正极的载体的性能。研究表明由稻谷壳制备的硫/生物炭正极材料表现出最高的比容量和最优的循环稳定性。为了进一步改善其电性能,以SiO溶胶为模板制备了具有高孔隙率的稻谷壳生物炭,其多孔结构可有效抑制多硫化物的溶解。由此得到的硫/生物炭（硫含量为60%（w,质量分数））材料中的硫以无定型态均匀地分散在碳载体中。该材料表现出更优异的电化学性能:在0.2C(1C=1675 m A?g)倍率下,首周放电容量为1534.1 mAh?g,循环100周后仍可保持在783.7 mAh?g;倍率性能测试中,在2.0C倍率下,材料的可逆容量为485.3 mAh?g。supported by the National Natural Science Foundation of China(21373008)；Fundamental Research Funds for the Central Universities,China(20720160124)~~

中医诊治多囊卵巢综合征证型和用药规律文献讨论

目的:探讨多囊卵巢综合征的中医证治规律。方法:检索1999年至2013年中国期刊全文数据库(CnkI)收录的中医治疗多囊卵巢综合征的文献,对所选文献中的证型和用药进行统计归类分析。结果:最终筛选合格文献63篇、证型26个,常见证型有脾肾阳虚、痰瘀互结、肾虚血瘀、肾阴虚、痰湿阻滞、肾虚痰湿共占56.33%。证素有10个,其中虚证占81.88%,主要有阳虚、气虚、阴虚、阴阳两虚和血虚;实证占18.12%,主要有痰湿、痰瘀、气滞、血瘀,其中痰湿占总证候要素的6.97%。用药148味,按功效归为15类,补虚药使用最多占比39.83%,其次是化痰湿药、活血药、理气药,前4类共占比84.19%。结论:本病病机复杂,以肾虚为主,痰湿瘀阻为标,肝病是重要发病环节,故补肾调肝兼祛痰化瘀是治疗大法

学术图书馆学科导航门户资源类型表的设定

在对DC和ROADS以及SOSIG、INFOMINE和CSDL等大型学科导航门户对资源类型设定的调研基础上,提出了区分和设定资源类型的原则,并根据该原则设定了适合于CALIS学科导航门户建设的资源类型表

Effects of transfection with acidic fibroblast growth factor by electroporation on skeletal muscle satellite cells

背景:课题组早期研究表明体外一定剂量酸性成纤维细胞生长因子对骨骼肌卫星细胞增殖有促进作用。目的:进一步验证电穿孔转染酸性成纤维细胞生长因子基因对骨骼肌卫星细胞生长、增殖及分化的影响。方法:原代培养、纯化骨骼肌卫星细胞,将带有酸性成纤维细胞生长因子基因的质粒P SECTAg-gfP-A fgf通过电转染的方法转染大鼠骨骼肌卫星细胞,荧光显微镜观察绿色荧光蛋白的表达情况并计算转染率,以流式细胞仪分析转染后细胞周期,绘制细胞生长曲线,观察转染后肌管形成情况,WESTErn blOTIng检测酸性成纤维细胞生长因子基因的表达。结果与结论:1免疫细胞化学检测:骨骼肌肌动蛋白呈阳性表达。2转染效率:P SECTAg-A fgf质粒电转染12 H后即可看见散在发绿色荧光的卫星细胞,72-96 H达高峰,阳性表达率约90%。3细胞周期检测:电转染后S期所占的百分比明显多于未转染对照组(P<0.05)。4细胞生长曲线检测:电转染细胞接种后第3天进入对数生长期,第5天后开始减少。5分化能力观察:电转染组肌管较未转染对照组明显减少,老化细胞较少。6WESTErn-blOT:酸性成纤维细胞生长因子基因在转染骨骼肌卫星细胞中表达。结果表明,通过电穿孔法可以将酸性成纤维细胞生长因子基因转染进骨骼肌卫星细胞并获得高效持久的表达,并有促进骨骼肌卫星细胞增殖及抑制分化为肌管的作用。BACKGROUND: Previous studies have shown that a certain dose of acidic fibroblast growth factor can promote skeletal muscle satellite cell proliferation in vitro.OBJECTIVE: To investigate the effects of transfection with acidic fibroblast growth factor by electroporation on growth, proliferation and differentiation of skeletal muscle satellite cells.METHODS: Skeletal muscle satellite cells were cultured and purified, and then transfected with plasmid p Sectag-GFP-a FGF by electroporation.The expression of green fluorescent protein was observed under fluorescence microscope, and the transfection efficiency was calculated.After transfection, cell cycle was analyzed by flow cytometry to draw the growth curve of skeletal muscle satellite cells.Western blot assay was employed to measure protein level of acidic fibroblast growth factor.RESULTS AND CONCLUSION:(1) Immunocytochemistry detection: The skeletal muscle satellite cells were positive for a-sarcomeric actin.(2) Transfection efficiency: At 12 hours after transfection with p Sectag-a FGF, several cells showed green fluorescence, and the green fluorescent expression reached the peak at 72-96 hours after transfection with a positive rate of about 90%.(3) Cell cycle: After electrotransfection, the proportion of cells at S phase in the electroporation group was higher than that in the control group(P < 0.05).(4) Cell growth curve: At 3 days after electrotransfection, the cells entered logarithmic growth phase but the proliferation slowed down at 5 days.(5) Differentiation capacity: There were fewer myotubes and aging cells in the electroporation group than the control group.(6) Western blot assay: Acidic fibroblast growth factor protein was highly expressed in the cells transfected with target gene detected by western blot assay.These findings indicate that by using electroporation method, acidic fibroblast growth factor can be transferred into skeletal muscle satellite cells and have a high-efficiency and long-term expression, which can promote the proliferation of skeletal muscle satellite cells and inhibit formation of myotubes

The effect of nurse initiated patient/family education strategy on people with schizophrenia in Beijing

2004-2005 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Abundance and production of bacteria and their correlations with environmental factor

以2014年8月南海北部海水样品为研究对象,利用平板计数法和流式细胞仪计数法对南海北部表层和垂直海域可培养细菌和细菌总数分布状况进行研究,对细菌; 生产力进行测定,并结合环境因子进行相关性分析。结果表明:珠江口到南海北部海域,水平方向可培养细菌总数变化范围是3.70*10~2 ~; 1.42*10~3 CFU/mL,细菌总数变化范围是5.12 * 10~5 ~1.61 * 10~6; cells/mL,细菌生产力的变化范围是0.03 ~0.40 mg/m~3/h;垂直方向上可培养细菌变化范围是1.08 *10~3 ~9.00; *10~3 CFU/mL,细菌生产力变化范围是0.01 ~0.08; mg/m~3/h,其中表层海水中的细菌生产力明显高于底层。与环境因子相关性分析表明,水平方向上,影响南海北部表层海水细菌总数和细菌生产力的主要因; 素是温度、盐度、硝酸盐(NO_3-N)、硅酸盐(SiO_3-Si)、亚硝酸盐(NO_2-N)和磷酸盐(PO_4-P)(P<0.05);垂直方向上; ,影响南海北部可培养细菌总数的主要因素是NO_2-N(P<0.05),影响细菌生产力的主要影响因素是温度和盐度(P; <0.05)。可见,南海北部表层海水中细菌总数高于可培养细菌总数2; ~3个数量级,表明该海域表层海水存在大量不可培养细菌;细菌的生命活动在海水表层相较底层更为活跃。Distribution of abundance and production of bacteria and their; correlations with environmental factor were investigated, using plate; count method and flow cytometry, in the surface and vertical waters of; the northern south China sea in August 2014. The cultivable bacterial; abundance ranged from 3.70 * 10~2 CFU/mL to 1.42 * 10~3 CFU/ mL, the; total bacterial abundance ranged from 5.12 * 10~5 cells/mL to 1.61 *; 10~6 cells/mL and the bacterial productions varied from 0.03 mg/m~3/h to; 0.40 mg/m~3/h in horizontal distribution. In vertical distribution, the; cultivable bacterial abundance ranged from 1.08 * 10~3 CFU/mL to 9.00 *; 10~3 CFU/mL and the bacterial productions varied from 0.01 mg/m~3/h to; 0.08 mg/m~3/h, respectively. The correlation analysis results showed; that the environmental factors affecting the abundance of total bacteria; and bacterial productivity included temperature, salinity,; nitrate(NO_3-N),silicate (SiO_3-Si), nitrite (NO_2-N),and phosphate; (PO_4-P) (P<0.05). NO_2-N was the main influencing factor to cultivable; bacteria abundance (P < 0.05), while bacterial productivity was highly; correlated with temperature and salinity in vertical distribution of; northern south China sea (P < 0.05). The total bacterial abundance was 2; ~ 3 orders of magnitude higher than cultivable bacteria, suggesting that; there were many uncultured bacterium in surface water of northern south; China sea and bacterial activities in the surface maybe more active than; in the bottom.全球变化与海气交互作用; 国家重点研发计划全球变化及应对专项; 海洋公益性行业科研专

Diversity of CO_2 fixation gene in the surface waters of northern South China Sea in the Calvin cycle

为揭示南海北部表层海水中参与卡尔文循环的固碳基因多样性及其与环境因子的关系,本研究以卡尔文循环中的关键酶核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubis CO)的Ⅰ、Ⅱ型基因(cbbL、cbbM)作为分子标记,采用Illumina Miseq高通量测序技术对海水中的固碳基因多样性进行分析,并结合多元统计分析的方法,探讨了固碳基因多样性与环境因子的关系.结果显示,在南海北部表层海水中,含cbbL基因固碳基因主要归属于变形菌门(Proteobacteria)、蓝藻门(Cyanobacteria)和厚壁菌门(Firmicutes),其中,优势亚群分别是γ-变形菌亚门(45.3%)、蓝细菌(30.9%)和β-变形杆菌亚门(23.8%);而含cbbM基因的固碳菌群未检测到;近岸的A9站位与其他站位物种组成有一定的差异,异着色菌属(Allochromatium)、硫杆菌属(Thiobacillus)和硫单胞菌属(Thiohalomonas)为其特有菌属.相关性和冗余分析(RDA)结果显示,含cbbL基因的固碳基因丰度与水温、盐度呈显著负相关(p<0.01),与硝酸盐、亚硝酸盐、硅酸盐呈显著正相关(p<0.01).To elucidate the diversity of CO_2 fixation and its relationship with environmental factors in surface water of northern South China Sea,the coding gene form I( cbbL) and form II( cbbM)( that encodes Ribulose-1,5-biphosphate carboxylase/oxygenase( Rubis CO) were chosen as biomarkers in Calvin-Benson-Bassham cycle( a common carbon fixation pathway for chemolithoautotrophic microorganisms) to analyze the carbon sequestration gene diversity. The Illumina Miseq sequencing method and multivariate statistical analysis were employed. The cbbM gene was not detected in any of samples,while the cbbL gene was found in all samples. The cbbL-containing genetic communities were dominated by Proteobacteria,Cyanobacteria and Firmicutes,in which γ-Proteobacteria( 45. 3%),Cyanobacteria( 30. 9%),β-Proteobacteria( 23. 8%) were predominant subpopulation. There were differences between the nearshore station of A9 with other stations,in which Allochromatiu,Thiobacillus and Thiohalomonas were unique species in A9 station.Relativity and redundancy analysis suggest that water temperature, salinity, nitrate, nitrite, silicate were highly statistically significant factors.Temperature and salinity were positively( p < 0. 01),while the other three factors negatively influenced CO_2 fixation gene of cbbL gene abundance( p <0. 01).全球变化与海气交互作用专项(No.GASI-03-01-02-05);; 海洋公益性行业科研专项(No.201305030,201405007)~

Six low-dimensional silver(I) coordination complexes derived from 2-aminobenzonitrile and carboxylates

通讯作者地址: Huang, RB (通讯作者),Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] new Ag(I) coordination complexes, [Ag(abn)(4-cba)](2) (1), [Ag(abn)(3-cba)](2) (2), [Ag(abn)(fba)](2) (3), [Ag(abn)(3-mba)](2) (4), [Ag(abn)(moba)](2) (5), [Ag(abn)(2)(4-mba)](n) (6), (abn = 2-aminobenzonitrile, 4-cbaH = 4-chlorobenzoic acid, 3-cbaH = 3-chlorobenzoic acid, Hfba = 3-fluorobenzoic acid, 3-mbaH = 3-methylbenzoic acid, Hmoba = 2-methoxybenzoic acid and 4-mbaH = 4-methylbenzoic acid), have been synthesized and characterized by elemental analysis, IR spectra and single crystal X-ray diffraction. Complexes 1-4 are similar discrete binuclear Ag(I) motif containing monodentate mu(1)-N-amino abn and mu(2)-eta(1):eta(1) carboxylates. The complementary N-amino-H center dot center dot center dot N-cyano hydrogen bonds link the binuclear motifs into 1D chain which is further extended into 2D supramolecular sheet by N-amino-H center dot center dot center dot O-carboxyl hydrogen bonds. Notably, the electronic effect of substituents in 1-4 has deeply influence on the Ag center dot center dot center dot Ag interaction. Complex 5 is also a discrete binuclear Ag(I) motif but contains a monodentate mu(1)-N-cyano abn ligand. The 1D chain was observed in complex 6 where two different abn ligands with mu(1)-N-cyano and mu(2)-N-cyano, N-amino coordination modes coexist and 4-mba is a chelating mu(1)-eta(1):eta(1) ligand. The results show that the different structures are predominantly attributed to the various ancillary carboxylate ligands and diverse coordination modes of abn ligand. Moreover, the emissive behaviors of them are discussed. (C) 2012 Elsevier B.V. All rights reserved.National Natural Science Foundation of China 21021061 21071118 MSTC 2007CB81530

正则多部竞赛图中的分量共轭圈

竞赛图的共轭圈问题已经完全解决,而关于多部有向图的共轭圈问题仍然是一个open问题。Yeo于1999年提出正则多部竞赛图包含共轭圈的猜想。论文根据分量共轭圈(componentwisecomplementarycycles)的定义,证明了:如果D是一个正则的n-部竞赛图(n≥4),则D包含一对分量共轭圈C1和C2,除非它同构于T71。这对于解决Yeo的猜想和多部有向图的共轭圈问题有一定的意义