Detection of isoniazid-resistant mutations in Mycobacterium tuberculosis by fluorescent PCR probes and melt-curve analysis

目的研究荧光PCr熔解曲线法检测结核分枝杆菌耐异烟肼突变方法,评价检测耐多药结核病的能力。方法对结核病人临床分离结核分枝杆菌使用荧光PCr熔解曲线法检测,与金标准传统药物敏感性试验比较,获得该方法的灵敏度、特异度和一致率。结果检测408例结核病人临床分离培养样本,药敏试验检出278例异烟肼敏感标本,130例异烟肼耐药标本;荧光PCr探针熔解曲线法检出异烟肼敏感和耐药标本281例、127例,灵敏度为87.69%,特异性为95.32%,一致率为92.89%。结论荧光PCr探针熔解曲线法检测速度快速、特异性强、灵敏度较高,可用于结核分枝杆菌耐异烟肼突变的快速检测,适于耐多药结核病的快速筛查。OBJECTIVE The study explored the features of fluorescent PCR / melt-curves analysis in the detection of isoniazid-resistant mutations in Mycobacterium tuberculosis, so to evaluate the capability of the method in the identification of multidrug-resistant tuberculosis.METHODS Mycobacterium tuberculosis isolated from tuberculosis patients were subjected to fluorescent PCR / melt-curves analysis and gold standard conventional drug susceptibility test.The results were compared to obtain the sensitivity, specificity, and consistency of the new method.RESULTS Among the 408 culture samples isolated from tuberculosis patients, drug susceptibility test indicated 278 isoniazid-sensitive and 130 isoniazid-resistant specimens, and fluorescent PCR / melt-curves analysis revealed 281 and 127 specimens, respectively.Consequently, sensitivity, specificity,and consistency of the fluorescent PCR / melt-curves analysis were 87.69%, 95.32%, and 96.32%, respectively.CONCLUSION Fluorescent PCR / melt-curves analysis is time-efficient and high in specificity and sensitivity, and may be used in the rapid detection of isoniazid-resistant mutations in Mycobacterium tuberculosis and rapid screening of multidrug-resistant tuberculosis.“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项资助项目(2008ZX10003-004

Cognitive Function and Activity of Daily Living of the Old Elderly of Zhuang Nationality in Bama County

目的:了解广西巴马县壮族长寿老人认知功能及日常生活自理能力. 方法:采用简易智力状态检查(MMSE)、日常生活能力量表(ADL)入户调查. 结果:85～89、90～94、95～104岁组MMSE总分分别为24.39±2.36;23.5±2.7;22.3±3.1,符合认知下降诊断者分别占3%;9.3%和13.7%.长寿老人随增龄主要表现为短时记忆、注意力和计算力下降明显(P＜0.05).ADL方面,各组自理能力功能丧失率分别为6%;12.5%和31%,其中85～89岁与95～104岁组比较差异显著(P＜0.05),而在ADL所有项目中,仅85～89岁与95～104岁组在处理自已钱财功能丧失有显著差异(P＜0.05). 结论:巴马长寿老人总体认知功能、自理能力较其他老年人群保持好;增龄仍然是长寿老人认知及日常活动能力下降的重要因素;长寿老人ADL和MMSE相关明显

分子筛笼内铂羰基簇合物在CO＋NO反应中的催化研究

研究了ＮａＹ分子筛超笼内包容的Ｐｔ羰基簇合物催化剂在ＣＯ＋ＮＯ反应中的催化性能。「Ｐｔ１２（ＣＯ）２４」＾２－／ＮａＹ和「Ｐｔ９（ＣＯ）１３」＾２－／ＮａＹ在此反应中表现出比较高的活性。Ｎ２Ｏ被认为是ＣＯ＋ＮＯ反应的中间产物，同时笼内羰基簇合物还表现了比ＳｉＯ２负载的簇合物好的热稳定性，动力学研究表明，在「Ｐｔ１２（ＣＯ）２４」＾２－／ＮａＹ上，ＮＯ被还原和Ｎ２Ｏ生成的反应级数，相对于ＣＯ分压为

3a分子筛对四氢呋喃水合物生成过程的影响

用显微镜从微观角度研究了0℃以下常压四氢呋喃-水体系中加入3A分子筛粉后THF水合物的生成过程，结果表明3A分子筛的存在能诱发水合物的成核，促进THF水合物的生成，提高THF水合物的生长速率；有3A分子筛存在时，THF水合物晶体的生长速率介于0．01～0．05gm／s之间；相对于无3A分子筛存在的体系，THF水合物晶体的生长速率提高约4nm／s；在相同条件下，THF水合物晶体长成后大晶体还会发生部分变化和重组