散发型戊型肝炎与急性乙肝临床特征比较

目的比较散发型急性戊型肝炎与乙肝临床特征。方法分析戊肝和急性乙肝间年龄、性别、肝脏损伤、症状体征以及病程的差异。结果急性肝炎病例中,戊型肝炎最常见(28.0%),急性乙肝次之(9.2%);戊肝平均发病年龄为56.3±13.1岁,急性乙肝为43.0±12.5岁,平均发病年龄戊肝较急性乙肝大(t=4.4723,P<0.0001),均为男性多发;两者常见的临床症状和体征基本相当,但戊肝患者黄疸症状更多见(P<0.05);戊肝病程较急性乙肝长(t=3.7249,P=0.0003);通过年龄性别进行1:1匹配分析,戊肝比急性乙肝对肝脏的损伤程度严重(t=3.5978,P=0.0019)。结论戊肝多见于中老年,急性乙肝多见于中青年,均为男性多于女性。临床特征比较戊肝较急性乙肝更为严重

Determination of Phosphoamino Acids by Fluorescence Quenching

建立了通过磷酰化氨基酸水解产生无机磷酸盐猝灭铽离子-钛铁试剂络合物(Tb3+-TR)荧光探针的间接荧光法测定三种磷酰化氨基酸。在最佳实验条件下,方法测定磷酰化丝氨酸(P-Ser)、磷酰化苏氨酸(P-Thr)和磷酰化酪氨酸(P-Tyr)的线性范围分别为5.0×10-8~5.0×10-7mol/L、5.0×10-8~6.0×10-7mol/L、5.0×10-8~6.0×10-7mol/L;检出限分别为2.06×10-8mol/L、1.13×10-8mol/L和1.74×10-9mol/L。该方法最后用于卵黄高磷蛋白中含磷量的测定,取得定量结果。A sensitive detection method for phosphoamino acids(P-Ser,P-Thr and P-Tyr) by fluorescence quenching has been developed.Under the optimal conditions,the linear ranges are 5.0×10-8 ~5.0×10-7 mol/L for P-Ser,5.0×10-8 ~6.0×10-7 mol/L for P-Thr and 5.0×10-8 ~6.0×10-7 mol/L for P-Tyr,respectively.The detection limits are 2.06×10-8 mol/L,1.13×10-8 mol/L and 1.74×10-9 mol/L,respectively.The method has been applied to the determination of P-Ser in phosvitin.福建省自然科学基金(No.D0010001

Epidemiological characteristics of sporadic hepatitis E in the rural area of Dongtai,Jiangsu Province,China

目的分析2008-2011年江苏省某农村地区戊型病毒性肝炎流行病学特征。方法利用已建立的疑似肝炎症状监测系统主动发现戊型病毒性肝炎患者,并对其流行病学特征进行分析。结果 2008-2011年戊型肝炎所占急性病毒性肝炎比例为27.29%,成为第2个主要病毒性肝炎。戊型肝炎年平均发病率2.2/万,戊肝发病全年散发,冬春季节高发;男性发病率高于女性,发病率随年龄增长逐渐升高,发病平均年龄(55.48±14.32)岁。戊型肝炎病毒株HEV1、4型并存,以HEV4型为主(95.6%)。结论本地戊肝呈散发,中老年高发,男性多于女性,以HEV4型为主。In order to analyze the epidemiologic characteristics of hepatitis E in the rural area of Jiangsu Province during the period of2008-2011,an established monitoring system of suspected hepatitis was used to detect patients of hepatitis E.The study showed that hepatitis E cases accounted for 27.29% of the total acute hepatitis cases during this period,which indicated hepatitis E had become the second major hepatitis in the rural area of Jiangsu Province.The average annual incidence rate of hepatitis E was around 0.22‰.Hepatitis E was sporadic throughout the whole year,with a higher incidence in spring and winter.The morbidity of hepatitis E was higher in men than that in women and was rising with age.The average age of all cases is 55.48±14.32 years.The research also revealed that HEV of genotype 4was the major genotype in the rural area of Jiangsu Province which accounted for 95.6%,while the proportion of genotype 1was 4.4%.In conclusion,hepatitis E in the rural area of Jiangsu Province is sporadic throughout the whole year,and HEV of genotype 4is the major genotype.The incidence of hepatitis E is higher in the middle-aged and old people and it is higher in men than that in women.国家自然科学基金项目(81373061);; 江苏省科技支撑计划(社会发展)项目(BE2013723)资助~