建立了通过磷酰化氨基酸水解产生无机磷酸盐猝灭铽离子-钛铁试剂络合物(Tb3+-TR)荧光探针的间接荧光法测定三种磷酰化氨基酸。在最佳实验条件下,方法测定磷酰化丝氨酸(P-Ser)、磷酰化苏氨酸(P-Thr)和磷酰化酪氨酸(P-Tyr)的线性范围分别为5.0×10-8~5.0×10-7mol/L、5.0×10-8~6.0×10-7mol/L、5.0×10-8~6.0×10-7mol/L;检出限分别为2.06×10-8mol/L、1.13×10-8mol/L和1.74×10-9mol/L。该方法最后用于卵黄高磷蛋白中含磷量的测定,取得定量结果。A sensitive detection method for phosphoamino acids(P-Ser,P-Thr and P-Tyr) by fluorescence quenching has been developed.Under the optimal conditions,the linear ranges are 5.0×10-8 ~5.0×10-7 mol/L for P-Ser,5.0×10-8 ~6.0×10-7 mol/L for P-Thr and 5.0×10-8 ~6.0×10-7 mol/L for P-Tyr,respectively.The detection limits are 2.06×10-8 mol/L,1.13×10-8 mol/L and 1.74×10-9 mol/L,respectively.The method has been applied to the determination of P-Ser in phosvitin.福建省自然科学基金(No.D0010001
